BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0012.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yvgN [R] COG0656 Aldo/keto reductases, related to diketogulon... 341 9e-94 BH3849 [R] COG0656 Aldo/keto reductases, related to diketogulona... 324 1e-88 lin0819 [R] COG0656 Aldo/keto reductases, related to diketogulon... 316 3e-86 BH2158 [R] COG0656 Aldo/keto reductases, related to diketogulona... 313 2e-85 BS_ytbE [R] COG0656 Aldo/keto reductases, related to diketogulon... 302 3e-82 >BS_yvgN [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 341 bits (874), Expect = 9e-94, Method: Compositional matrix adjust. Identities = 12/272 (4%), Positives = 45/272 (16%), Gaps = 1/272 (0%) Query: 4 IADVTILNNGIAMPRFGLGTYKAE-GEEVARAVATALELGYRSIDTAAVYGNEEEVGRSI 62 + L + + A+ + + + Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 63 AASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKATWR 122 + V ++ + + + + Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 123 ALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCASRQI 182 L+ R + + + ++ + + R + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 183 QIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKENSE 242 + + L + +V L Q V + + Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGK 244 Query: 243 IFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 + A + + + Sbjct: 245 YLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA 276 >BH3849 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 324 bits (830), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 14/275 (5%), Positives = 47/275 (17%), Gaps = 1/275 (0%) Query: 1 MKHIADVTILNNGIAMPRFGLGTYKA-EGEEVARAVATALELGYRSIDTAAVYGNEEEVG 59 H + L + E + A+ + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 60 RSIAASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKA 119 + + + V ++ + + + + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 120 TWRALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCAS 179 L+ R + + + ++ + + R + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 180 RQIQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKE 239 + + L + +V L Q V + Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 240 NSEIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 + + A + + + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA 276 >lin0819 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 316 bits (809), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 15/273 (5%), Positives = 47/273 (17%), Gaps = 1/273 (0%) Query: 3 HIADVTILNNGIAMPRFGLGTYKAE-GEEVARAVATALELGYRSIDTAAVYGNEEEVGRS 61 + + ++ G T+ + E A A ++ A + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 62 IAASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKATW 121 + + + + K + D + Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 122 RALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCASRQ 181 + + + F L ++ S + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 182 IQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKENS 241 L D + I + + + + + Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 242 EIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 + + + R + + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV 274 >BH2158 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 313 bits (801), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 13/268 (4%), Positives = 47/268 (17%), Gaps = 1/268 (0%) Query: 8 TILNNGIAMPRFGLGTYK-AEGEEVARAVATALELGYRSIDTAAVYGNEEEVGRSIAASG 66 + L + +E + A+ + + + + Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 67 VPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKYKATWRALER 126 V ++ + + + + L+ Sbjct: 69 VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL 128 Query: 127 LYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYCASRQIQIEA 186 R + + + ++ + + R + + Sbjct: 129 YQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMR 188 Query: 187 WAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRIKENSEIFDF 246 + L + +V L Q V + + + Sbjct: 189 YLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNG 248 Query: 247 ELSAEELSAISGLDAGERIGSDPDKLLF 274 A + + + Sbjct: 249 AKPAGARNTLFSRFTRYSGEQTQKAVAA 276 >BS_ytbE [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 302 bits (774), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 10/277 (3%), Positives = 44/277 (15%), Gaps = 5/277 (1%) Query: 3 HIADVTILNNGIAMPRFGLGTYKAE-GEEVARAVATALELGYRSIDTAAVYGNEEEVGRS 61 H + L + + A+ + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 62 I----AASGVPRDSLFVTTKVWNSDQGYDETLRAFETSSKKLGLDVIDLYLIHWPGMDKY 117 + + + + + + G + L I L + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 118 KATWRALERLYAEGRVRAIGVSNFQIHHLEELRKDSETVPAVNQVELHPRFIQKELHDYC 177 + + ++ Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 178 ASRQIQIEAWAPLMKGRLQDNELLHGIAGKHGKTVSQVILRWGLQNGIVIIPKSVTASRI 237 + R+ + + + + + + ++ + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 238 KENSEIFDFELSAEELSAISGLDAGERIGSDPDKLLF 274 K + S + + + + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDI 280 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,386,380 Number of extensions: 841419 Number of successful extensions: 15935 Number of sequences better than 1.0e-05: 159 Number of HSP's gapped: 15690 Number of HSP's successfully gapped: 160 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)