BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0005.p (209 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1828 [R] COG4122 Predicted O-methyltransferase 159 4e-39 mlr0279 [R] COG4122 Predicted O-methyltransferase 145 5e-35 Rv0187 [R] COG4122 Predicted O-methyltransferase 130 2e-30 MT0197 [R] COG4122 Predicted O-methyltransferase 130 2e-30 PA1200 [R] COG4122 Predicted O-methyltransferase 129 4e-30 >BH1828 [R] COG4122 Predicted O-methyltransferase Length = 223 Score = 159 bits (401), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 79/209 (37%), Positives = 127/209 (60%), Gaps = 6/209 (2%) Query: 7 YSEQLYTEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSG 66 +SE+L+ D ++ V +A + + +P + +AP G+ L +L ILEIG LGGYS Sbjct: 14 FSEKLHAFDPVMDWVLEANEKAELPPIDVAPNQGKFLYLLAKLKGVKTILEIGTLGGYSS 73 Query: 67 ICLCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEG-RT 125 I + R L GRL +LE ++A +A ++++AG ++ +GPAL++L L+ +G + Sbjct: 74 IWMARALPDDGRLVTLEFSEKHAAIAQSNMKKAGVDGKIDVIIGPALETLPTLKDKGFSS 133 Query: 126 FDFFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEM 185 FD FIDADK N P+YLE+A+KL+ PG +I GDN+ G LN E P+++ +R+F + Sbjct: 134 FDMIFIDADKPNNPHYLEWALKLSTPGTLIVGDNVVRNGNVLNKESADPSIKGIRQFIHL 193 Query: 186 IANDERLTSTLLPA-----YDGLALAVVK 209 ++ + R+ ST + YDG + VVK Sbjct: 194 LSEEPRIESTAIQTVGIKGYDGFVVGVVK 222 >mlr0279 [R] COG4122 Predicted O-methyltransferase Length = 301 Score = 145 bits (365), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 19/207 (9%), Positives = 42/207 (20%), Gaps = 6/207 (2%) Query: 9 EQLYTEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSGIC 68 + + LL K S + + + A ++ + G Sbjct: 15 GRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAI 74 Query: 69 LCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEGR-TFD 127 A + R + + + + D Sbjct: 75 NRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKAD 134 Query: 128 FFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEMIA 187 + N+L+ A G + +R A + P R +A Sbjct: 135 LLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMA 194 Query: 188 NDERLTSTLL-----PAYDGLALAVVK 209 ++ + Y Sbjct: 195 SEIIREKLMRFLGAELPYSVTVEIERF 221 >Rv0187 [R] COG4122 Predicted O-methyltransferase Length = 220 Score = 130 bits (326), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 75/184 (40%), Positives = 111/184 (60%), Gaps = 8/184 (4%) Query: 31 PEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSGICLCRGLSKAGRLTSLELKAEYAE 90 P ++++ G+ L +L A ++ R+LEIG LGG+S I L RG GR+ +LE + ++AE Sbjct: 40 PRIAVSAQQGKFLCLLAGAIQARRVLEIGTLGGFSTIWLARGAGPQGRVVTLEYQPKHAE 99 Query: 91 LAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEGRTFDFFFIDADKENYPNYLEYAIKLAA 150 +A +L+ AG + VE +GPALD+L L G FD FIDADKEN Y+++AI+LA Sbjct: 100 VARVNLQRAGVADRVEVVVGPALDTLPTL--AGGPFDLVFIDADKENNVAYIQWAIRLAR 157 Query: 151 PGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEMIANDERLTSTLL-----PAYDGLAL 205 GAVI DN+ +RG + AE + A RR +M+ L +T + +DG AL Sbjct: 158 RGAVIVVDNV-IRGGGILAESDDADAVAARRTLQMMGEHPGLDATAIQTVGRKGWDGFAL 216 Query: 206 AVVK 209 A+V+ Sbjct: 217 ALVR 220 >MT0197 [R] COG4122 Predicted O-methyltransferase Length = 218 Score = 130 bits (326), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 75/184 (40%), Positives = 111/184 (60%), Gaps = 8/184 (4%) Query: 31 PEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSGICLCRGLSKAGRLTSLELKAEYAE 90 P ++++ G+ L +L A ++ R+LEIG LGG+S I L RG GR+ +LE + ++AE Sbjct: 38 PRIAVSAQQGKFLCLLAGAIQARRVLEIGTLGGFSTIWLARGAGPQGRVVTLEYQPKHAE 97 Query: 91 LAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEGRTFDFFFIDADKENYPNYLEYAIKLAA 150 +A +L+ AG + VE +GPALD+L L G FD FIDADKEN Y+++AI+LA Sbjct: 98 VARVNLQRAGVADRVEVVVGPALDTLPTL--AGGPFDLVFIDADKENNVAYIQWAIRLAR 155 Query: 151 PGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEMIANDERLTSTLL-----PAYDGLAL 205 GAVI DN+ +RG + AE + A RR +M+ L +T + +DG AL Sbjct: 156 RGAVIVVDNV-IRGGGILAESDDADAVAARRTLQMMGEHPGLDATAIQTVGRKGWDGFAL 214 Query: 206 AVVK 209 A+V+ Sbjct: 215 ALVR 218 >PA1200 [R] COG4122 Predicted O-methyltransferase Length = 220 Score = 129 bits (323), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 70/195 (35%), Positives = 106/195 (54%), Gaps = 2/195 (1%) Query: 14 EDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSGICLCRGL 73 E L+ ++++ IAP G+ + +L+ + R LE+G GYS +C+ L Sbjct: 23 ETPLMTRLREETAKLPNARWQIAPEQGQFIALLLTLIGARRALEVGTFTGYSALCMATAL 82 Query: 74 SKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEGRT--FDFFFI 131 + GRLT +L +Y A R+ EA E +E +LGPAL++L+ L EG FD FI Sbjct: 83 GEQGRLTCCDLPGDYHLTARRYWREAAVEERIELRLGPALETLRALLDEGLAGQFDLAFI 142 Query: 132 DADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEMIANDER 191 DADK NYP YLE A+ L G ++ DN+ GR L A+ + +++ N + ND R Sbjct: 143 DADKANYPEYLEAALALVRQGGLVLFDNVLWSGRVLEAQPKSADTRGIQQLNLALKNDAR 202 Query: 192 LTSTLLPAYDGLALA 206 + +LLP DGL++ Sbjct: 203 VDYSLLPIGDGLSVC 217 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,272,031 Number of extensions: 631929 Number of successful extensions: 2550 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 2543 Number of HSP's successfully gapped: 10 Length of query: 209 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 107 Effective length of database: 26,614,363 Effective search space: 2847736841 Effective search space used: 2847736841 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 113 (48.1 bits)