BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0004.p (412 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yceE [T] COG2310 Uncharacterized proteins involved in stress ... 241 2e-63 L129379 [GEPR] COG0477 Permeases of the major facilitator superf... 234 2e-61 STM0382 [GEPR] COG0477 Permeases of the major facilitator superf... 224 2e-58 SP0972 [GEPR] COG0477 Permeases of the major facilitator superfa... 200 4e-51 SPy0501 [GEPR] COG0477 Permeases of the major facilitator superf... 185 1e-46 >BS_yceE [T] COG2310 Uncharacterized proteins involved in stress response, homologs of TerZ and putative cAMP-binding protein CABP1 Length = 408 Score = 241 bits (614), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 136/356 (38%), Positives = 211/356 (59%), Gaps = 4/356 (1%) Query: 13 TWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQG-DAIGLWAGLIFAANFMTSFL 71 WK NLIV W G FL A +++ PFL LY+ + LGV G A+ +W+G++F+ F+ S + Sbjct: 11 NWKRNLIVAWLGCFLTGAAFSLVMPFLPLYV-EQLGVTGHSALNMWSGIVFSITFLFSAI 69 Query: 72 FQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASIA 131 P WG LAD+ GRK+MLLRS+ GM +V+VLMG AQ WQ G + GF P + A Sbjct: 70 ASPFWGGLADRKGRKLMLLRSALGMGIVMVLMGLAQNIWQFLILRALLGLLGGFVPNANA 129 Query: 132 LVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXXX 191 L++ P+ + G+A+G + +G V+G +LGP+ GG LAD G RP+F+I Sbjct: 130 LIATQVPRNKSGWALGTLSTGGVSGALLGPMAGGLLADSYGLRPVFFITASVLILCFFVT 189 Query: 192 XXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYVE 251 ++EKF ++ + + E L + +LF T ++Q A + +L LYV Sbjct: 190 LFCIREKFQPVSKKEM--LHMREVVTSLKNPKLVLSLFVTTLIIQVATGSIAPILTLYVR 247 Query: 252 KLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQAF 311 +L G+ ++AF +G++++V GV+ +L++P LGKL D++G +IL ALI + L +IP ++ Sbjct: 248 ELAGNVSNVAFISGMIASVPGVAALLSAPRLGKLGDRIGPEKILITALIFSVLLLIPMSY 307 Query: 312 VTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 V + QL I+RFL+G G LLP+V L+ + + R F +N S +GN+ G Sbjct: 308 VQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSNQIAGRIFSYNQSFRDIGNVTG 363 Score = 53.5 bits (127), Expect = 6e-07, Method: Compositional matrix adjust. Identities = 42/160 (26%), Positives = 72/160 (45%), Gaps = 2/160 (1%) Query: 21 LWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLFQPLWGKLA 80 L+ ++ I P LTLY+ ++L + +G+I + + + L P GKL Sbjct: 224 LFVTTLIIQVATGSIAPILTLYV-RELAGNVSNVAFISGMIASVPGVAALLSAPRLGKLG 282 Query: 81 DKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISG-FNPASIALVSGNTPK 139 D+ G + +L+ + L+++ M + QTP Q G G PA L+ N+ Sbjct: 283 DRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQTLLVYNSSN 342 Query: 140 ARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYI 179 G QS G + GPL+G A++ G+R +F + Sbjct: 343 QIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLV 382 >L129379 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 398 Score = 234 bits (597), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 132/360 (36%), Positives = 202/360 (56%), Gaps = 10/360 (2%) Query: 12 ETWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFL 71 E+W+ NLIVLWFG F+ G ++I PF++LY++ + +W+GL+F++ F+ + Sbjct: 3 ESWQRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVLAI 62 Query: 72 FQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASIA 131 PLWG+LAD+ GRK+MLLR+S GMA+ I LM F WQ G SGF S+A Sbjct: 63 VSPLWGRLADQKGRKLMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNSMA 122 Query: 132 LVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXXX 191 L++ + PK + G + L+ +GSVAGT++GP+IGG L + GYR +F + G Sbjct: 123 LMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVNITGYRRVFSVTGIIMFLVFFLA 182 Query: 192 XXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPA----LFGVTFLLQFAMIAPMTLLP 247 VKE F E + +K++ +V PA +F T + Q + +L Sbjct: 183 LFFVKEIFKPVEKKN------MLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLS 236 Query: 248 LYVEKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFII 307 LYV++L +I F AG+V+A G+ ++A+P LG+L DKVG +IL + L + + Sbjct: 237 LYVQELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFS 296 Query: 308 PQAFVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 A T+VW LI++R L+GV +LPSV A++ +P R F +N S ++G G Sbjct: 297 ITAMTTNVWFLIVMRLLVGVSDAAILPSVQAILAKESPQAITGRIFSYNQSAQSIGAFAG 356 Score = 54.3 bits (129), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 42/158 (26%), Positives = 73/158 (46%), Gaps = 2/158 (1%) Query: 21 LWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLFQPLWGKLA 80 ++F + I P L+LY+ + + QG+ I AG++ AA + + + P G+L Sbjct: 217 MFFATLITQMTNQSINPVLSLYVQELMHGQGN-ITFMAGIVAAAPGIVTLVAAPFLGRLG 275 Query: 81 DKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNG-TISGFNPASIALVSGNTPK 139 DK G++ +L F +V + W G + + P+ A+++ +P+ Sbjct: 276 DKVGQRKILGFGLFFSLIVFSITAMTTNVWFLIVMRLLVGVSDAAILPSVQAILAKESPQ 335 Query: 140 ARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIF 177 A G QS G GPL+G A+A +I YR +F Sbjct: 336 AITGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVF 373 >STM0382 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 403 Score = 224 bits (571), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 132/357 (36%), Positives = 209/357 (58%), Gaps = 3/357 (0%) Query: 12 ETWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQG-DAIGLWAGLIFAANFMTSF 70 E+WKVNLI +WFG F ++ I PFL LY+++ LGV +A+ +W+GL F+ F+ S Sbjct: 2 ESWKVNLISVWFGCFFTGLAISQILPFLPLYISQ-LGVSSHEALSMWSGLTFSITFLISA 60 Query: 71 LFQPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASI 130 + P+WG LAD+ GRK+MLLR+S GMA+ I+L FA WQ G SG+ P ++ Sbjct: 61 IVSPMWGSLADRKGRKLMLLRASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAM 120 Query: 131 ALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXX 190 ALV+ P+ R G+A+ + + ++G I GPL+GG +AD IG R +F I Sbjct: 121 ALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADHIGLRAVFLITAMLLVVSFLV 180 Query: 191 XXXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYV 250 +KE R + ++S F L + +LF T ++Q + +L L++ Sbjct: 181 TLFLIKEGV-RPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFI 239 Query: 251 EKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQA 310 + + + +IAF +G++++V G+S ++++P LGKL D++G RIL LI A L + Sbjct: 240 KSMMPDSNNIAFLSGLIASVPGISALISAPRLGKLGDRIGTERILMATLICAVLLFFAMS 299 Query: 311 FVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 +VT+ +QL ++RFL+G G +LP+V L+ Y+ D R FG+N S + LGN++G Sbjct: 300 WVTTPFQLGLLRFLLGFADGAMLPAVQTLLVKYSSDQITGRIFGYNQSFMYLGNVVG 356 >SP0972 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 399 Score = 200 bits (508), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 128/357 (35%), Positives = 198/357 (55%), Gaps = 8/357 (2%) Query: 13 TWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLF 72 WK NL + WFG FL A ++++ PF+ +++ ++LGV + +AGL + + +++ LF Sbjct: 5 NWKDNLRIAWFGNFLTGASISLVVPFMPIFV-ENLGVGSQQVAFYAGLAISVSAISAALF 63 Query: 73 QPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTP--WQXXXXXXXNGTISGFNPASI 130 P+WG LADKYGRK M++R+ G+A+ I + G A P + NG +GF P + Sbjct: 64 SPIWGILADKYGRKPMMIRA--GLAMTITMGGLAFVPNIYWLIFLRLLNGVFAGFVPNAT 121 Query: 131 ALVSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXX 190 AL++ PK + G A+G + +G VAGT+ GP IGG +A+ G R +F +VG Sbjct: 122 ALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAELFGIRTVFLLVGSFLFLAAIL 181 Query: 191 XXXXVKEKFDRAEAAQVPQISVLEGFKELAKVPQLPALFGVTFLLQFAMIAPMTLLPLYV 250 +KE F A+ I E F + L LF +F++QF+ + +L LYV Sbjct: 182 TICFIKEDFQ--PVAKEKAIPTKELFTSVKYPYLLLNLFLTSFVIQFSAQSIGPILALYV 239 Query: 251 EKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQA 310 L G ++ F +G++ + G S+M+++ V+GKL DKVG HR+L A + + + A Sbjct: 240 RDL-GQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDKVGNHRLLVVAQFYSVIIYLLCA 298 Query: 311 FVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 +S QL + RFL G+ G L+P VNAL+ TP SR F FN LG ++G Sbjct: 299 NASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPKAGISRVFAFNQVFFYLGGVVG 355 Score = 56.2 bits (134), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 49/162 (30%), Positives = 78/162 (48%), Gaps = 3/162 (1%) Query: 18 LIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLFQPLWG 77 L+ L+ F++ I P L LY+ +DLG Q + + +GLI ++ +S + + G Sbjct: 214 LLNLFLTSFVIQFSAQSIGPILALYV-RDLG-QTENLLFVSGLIVSSMGFSSMMSAGVMG 271 Query: 78 KLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISG-FNPASIALVSGN 136 KL DK G +L+ + F ++ +L A +P Q G +G P AL+S Sbjct: 272 KLGDKVGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKM 331 Query: 137 TPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFY 178 TPKA + Q G ++GP+ G A+A GY +FY Sbjct: 332 TPKAGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFY 373 >SPy0501 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 397 Score = 185 bits (470), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 118/357 (33%), Positives = 190/357 (53%), Gaps = 8/357 (2%) Query: 13 TWKVNLIVLWFGQFLVNAGMTMITPFLTLYLAKDLGVQGDAIGLWAGLIFAANFMTSFLF 72 W+ NL V W G F A +++ PF+ LY+ ++LG + + +AGL A + S LF Sbjct: 2 NWRQNLKVAWLGNFFTGASFSLVMPFMALYV-ENLGTPTELVEYYAGLAVAVTALASALF 60 Query: 73 QPLWGKLADKYGRKIMLLRSSFGMALVIVLMGFAQTPWQXXXXXXXNGTISGFNPASIAL 132 P+WGKLAD+YGRK M+LR+SF M + + + G +G+ P + AL Sbjct: 61 APVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLILRLLTGVSAGYVPNATAL 120 Query: 133 VSGNTPKARMGFAMGLMQSGSVAGTILGPLIGGALADWIGYRPIFYIVGXXXXXXXXXXX 192 ++ PK G+A+G + +G AG ++GPL+GG LA+ +G R +F +VG Sbjct: 121 IASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLGIRQVFLLVGVILFLCSLMTA 180 Query: 193 XXVKEKFDRAEAAQ-VPQISVLEGFKELAKVPQLP-ALFGVTFLLQFAMIAPMTLLPLYV 250 VKE+F + +P +L+ K PQ+ LF + ++Q + + +L LY+ Sbjct: 181 VYVKEEFKPVRRFEMIPTKVILKQVKS----PQIMLGLFVTSMIIQISAQSVAPILSLYI 236 Query: 251 EKLHGSAVDIAFWAGVVSAVTGVSNMLASPVLGKLSDKVGAHRILTYALIGAALFIIPQA 310 L G ++ F +G+V + G S++ +S LGKL D+ G HR+L AL + + A Sbjct: 237 RHL-GQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGNHRLLLAALCYSFIMYFSSA 295 Query: 311 FVTSVWQLIIVRFLMGVFMGGLLPSVNALIRSYTPDGKESRAFGFNSSMLALGNMLG 367 + +QL ++RF G +G L+PS+N+L+ TP SR F +N LG ++G Sbjct: 296 LAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGISRVFAYNQMFSNLGQVIG 352 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,535,498 Number of extensions: 1039855 Number of successful extensions: 3874 Number of sequences better than 1.0e-05: 64 Number of HSP's gapped: 3842 Number of HSP's successfully gapped: 76 Length of query: 412 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 303 Effective length of database: 25,807,025 Effective search space: 7819528575 Effective search space used: 7819528575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)