BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0011.p (159 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1448 [J] COG0480 Translation elongation factors (GTPases) 121 3e-28 CAC0854 [J] COG0480 Translation elongation factors (GTPases) 112 2e-25 TM1651 [J] COG0480 Translation elongation factors (GTPases) 88 6e-18 all4338 [J] COG0480 Translation elongation factors (GTPases) 86 3e-17 RSc3022 [J] COG0480 Translation elongation factors (GTPases) 84 8e-17 >CAC1448 [J] COG0480 Translation elongation factors (GTPases) Length = 652 Score = 121 bits (304), Expect = 3e-28, Method: Composition-based stats. Identities = 59/157 (37%), Positives = 91/157 (57%) Query: 2 QGLYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVV 61 QGL+GWEVTD KVTLT G+ + P DF TPM M+ L R T LLEP+ + + + Sbjct: 495 QGLFGWEVTDAKVTLTCGEFFSPASTPSDFRNVTPMVFMEALYRAQTVLLEPLYEFELRI 554 Query: 62 PEENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSF 121 P++ + + DL MR F+ P++ G+ I G +P+ S +Y + ++SYTKG+ FT+ Sbjct: 555 PQDVLSKAIWDLETMRAIFDSPIVIGDEFSIRGLIPVENSKEYKMKIASYTKGKGMFTTK 614 Query: 122 FAGYEECPPDVRAERTRRGVNPLDQARYILSVRKALQ 158 F GY+E R + +PL++ Y+L A++ Sbjct: 615 FYGYKEVSAKFAKARPKTTYDPLNKKEYLLYKLNAIR 651 >CAC0854 [J] COG0480 Translation elongation factors (GTPases) Length = 644 Score = 112 bits (281), Expect = 2e-25, Method: Composition-based stats. Identities = 57/125 (45%), Positives = 75/125 (60%) Query: 4 LYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVVPE 63 L G VTD+KV L +G+HHV HT DF AT A+ GL LLEP +I V Sbjct: 471 LTGSVVTDIKVVLIDGRHHVKHTSGGDFREATLRALRQGLESTENVLLEPFYSFKIEVNS 530 Query: 64 ENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSFFA 123 ++ GRVM D+ +M G F PP ++ + +IEGR P+ +DYP SLSS+TKGR + F Sbjct: 531 DSMGRVMADINKMSGEFNPPYIRENKCVIEGRGPVVEFMDYPASLSSFTKGRGRISLNFD 590 Query: 124 GYEEC 128 GY+ C Sbjct: 591 GYDVC 595 >TM1651 [J] COG0480 Translation elongation factors (GTPases) Length = 683 Score = 87.8 bits (216), Expect = 6e-18, Method: Composition-based stats. Identities = 53/143 (37%), Positives = 77/143 (53%), Gaps = 10/143 (6%) Query: 4 LYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVVPE 63 L G+ VTD++V L +G +H + + F +A A G+ +LEPI++V + VPE Sbjct: 537 LAGYPVTDVRVILFDGSYHEVDSSDISFQIAAIQAFKKGMEAAKPVILEPIMEVEVFVPE 596 Query: 64 ENGGRVMNDLVQMRGT---FEPPVLQGERMI-IEGRLPLATSLDYPVSLSSYTKGRSTFT 119 EN G VM ++ RG EP G+ M+ ++ +PLA LD+ LSS T GR FT Sbjct: 597 ENAGDVMGEISSRRGRPLGMEP---SGKGMVKVKAEVPLAEMLDFSSKLSSITSGRGYFT 653 Query: 120 SFFAGYEECPPDVRA---ERTRR 139 F YE PP+++ E RR Sbjct: 654 MRFQRYEIVPPNIQEKIIEERRR 676 >all4338 [J] COG0480 Translation elongation factors (GTPases) Length = 692 Score = 85.5 bits (210), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 44/129 (34%), Positives = 69/129 (53%) Query: 4 LYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVVPE 63 L G+ + D+K TL G +H + + F +A MA+ + + + LLEP+++V + VPE Sbjct: 552 LAGYPLIDVKATLVHGSYHDVDSSEMAFKIAGSMALKEAVLKASPVLLEPMMKVEVEVPE 611 Query: 64 ENGGRVMNDLVQMRGTFEPPVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSFFA 123 + G V+ DL+ RG E + + ++PLAT Y + S T+GR FT F+ Sbjct: 612 DYIGNVIGDLISRRGQIESQSTEQGLAKVASKVPLATMFGYATDIRSKTQGRGIFTMEFS 671 Query: 124 GYEECPPDV 132 YEE P V Sbjct: 672 HYEEVPRSV 680 >RSc3022 [J] COG0480 Translation elongation factors (GTPases) Length = 703 Score = 84.0 bits (206), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 48/130 (36%), Positives = 70/130 (53%), Gaps = 1/130 (0%) Query: 4 LYGWEVTDLKVTLTEGQHHVWHTHPLDFAVATPMAIMDGLNRVGTKLLEPILQVRIVVPE 63 L G+ V D+KVTLT G +H ++ F +A MA D + + LLEP++ V + PE Sbjct: 563 LAGFPVVDVKVTLTFGSYHDVDSNENAFRMAGSMAFKDAMRKASPVLLEPMMAVEVETPE 622 Query: 64 ENGGRVMNDLVQMRGTFEP-PVLQGERMIIEGRLPLATSLDYPVSLSSYTKGRSTFTSFF 122 + G VM DL RG + + G II+ +PL+ Y SL S T+GR+T+T F Sbjct: 623 DYTGTVMGDLSSRRGIVQGMDDMVGGGKIIKAEVPLSEMFGYSTSLRSATQGRATYTMEF 682 Query: 123 AGYEECPPDV 132 Y E P ++ Sbjct: 683 KHYSEAPKNI 692 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,751,725 Number of extensions: 441093 Number of successful extensions: 2110 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 2100 Number of HSP's successfully gapped: 36 Length of query: 159 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 61 Effective length of database: 27,075,699 Effective search space: 1651617639 Effective search space used: 1651617639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 111 (47.4 bits)