BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0010.p (466 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1448 [J] COG0480 Translation elongation factors (GTPases) 330 3e-90 FN1556 [J] COG0480 Translation elongation factors (GTPases) 247 3e-65 TM1503 [J] COG0480 Translation elongation factors (GTPases) 246 7e-65 CAC0854 [J] COG0480 Translation elongation factors (GTPases) 244 1e-64 all4338 [J] COG0480 Translation elongation factors (GTPases) 244 2e-64 >CAC1448 [J] COG0480 Translation elongation factors (GTPases) Length = 652 Score = 330 bits (846), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 172/443 (38%), Positives = 269/443 (60%), Gaps = 19/443 (4%) Query: 10 INVGIFAHVDAGKTTTTEHILYESGRIKAVDSVDSGTALTDSMEVERQRGISVRAALASF 69 IN+GI AHVDAGKTT TE++LY SG IK+V SVDSG TDSME+ER+RGI+++++ SF Sbjct: 5 INIGIVAHVDAGKTTITENLLYYSGAIKSVGSVDSGNTQTDSMELERKRGITIKSSTISF 64 Query: 70 EWRGVQVNLVDTPGHVDFLSEVERSLRVMDCAVLILSAVEGVQAQSEMIWNALRKLGIPT 129 W V++N++DTPGHVDF+SEVERSL V+D A++++S VEG+Q+Q+ +++N L++L IPT Sbjct: 65 NWNDVKINIIDTPGHVDFISEVERSLSVLDGAIIVISGVEGIQSQTRILFNTLKELNIPT 124 Query: 130 LVFLNKMDRTGADPEAVLVQARTYLSGDILPVQQA--LGQEQEY----AGAVDLWAEAAD 183 ++F+NK+DR GAD V + + +S ++ +Q+A +G + Y + + +A D Sbjct: 125 IIFVNKLDRIGADFNKVFEEIKKNISNRVVRLQEAYDMGSKDVYIRNLSDICPINNDAVD 184 Query: 184 PXXXXXXXXXXXXXXXXXXXTYMSDTPLDLTAWKEQLKAGAAAGRWFPLVYGVAAKGLGI 243 Y+S + +E+L A G +P+ G AA GLGI Sbjct: 185 ---------VISDLDEVFLERYISGVEPNKEEVQEKLSLYAREGSLYPVFCGAAAIGLGI 235 Query: 244 TQLLDAMTDYFPRAGGNPQLPVSGIVYSIQRDKTMGRMAYVRLYQGMVRNRDTVMNYTQD 303 LLD + Y P AG + +SG+V+ I+R T + YVRL+ G V RD + +++ Sbjct: 236 GDLLDGICSYLPFAGDECEGNLSGVVFKIERTSTNEKKVYVRLFGGRVSVRDRIQVPSKE 295 Query: 304 VQGKVTQIRKVEGGRTEDVGTLEAGDIAVVYGLSGVKIGDVLGVPDAVPQEAKLAVPLLT 363 + KV +I +E GR + ++EAGDI ++YGL+ ++GDV+GV + + +A P L Sbjct: 296 IVEKVKKINSLENGRIIETQSIEAGDIGILYGLTDFQVGDVIGVSNNKIKNISIAKPTLK 355 Query: 364 VRVHWDAAVDEHKVIGAFQELADEDPLLDTQWLQDERELHIKVMGPIQLEILDSVLESRY 423 + + ++ A LA+EDPLL+ + + E+++ + G +Q+EIL S+L+ Y Sbjct: 356 TTISAIDKEENQELFKALTLLAEEDPLLELEMNDIDEEIYVNLFGEVQMEILSSILDGLY 415 Query: 424 GLKVTFGQPSVIYRETXXXKPRG 446 G+KV F IY+E KP+G Sbjct: 416 GIKVEFSNIQTIYKE----KPKG 434 >FN1556 [J] COG0480 Translation elongation factors (GTPases) Length = 693 Score = 247 bits (631), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 154/476 (32%), Positives = 238/476 (50%), Gaps = 47/476 (9%) Query: 11 NVGIFAHVDAGKTTTTEHILYESGRIKAVDSVDSGTALTDSMEVERQRGISVRAALASFE 70 NVGI AH+DAGKTTTTE IL+ +G + + V G A D ME E++RGI++ +A + Sbjct: 12 NVGIMAHIDAGKTTTTERILFYTGVERKLKEVHEGQATMDWMEQEQERGITITSAATTCF 71 Query: 71 WRGVQVNLVDTPGHVDFLSEVERSLRVMDCAVLILSAVEGVQAQSEMIWNALRKLGIPTL 130 W+G ++N++DTPGHVDF EVERSLRV+D AV + SAV+GVQ QSE +W K +P L Sbjct: 72 WKGHRINIIDTPGHVDFTVEVERSLRVLDGAVAVFSAVDGVQPQSETVWRQADKYKVPRL 131 Query: 131 VFLNKMDRTGADPEAVLVQARTYLSGDILPVQQALGQEQEYAGAVDL-------WAEAAD 183 F NKMDR GA+ + + + L + +P+Q +G E ++ G VDL W +D Sbjct: 132 AFFNKMDRIGANFDMCVSDIKEKLGSNPVPIQIPIGAEDQFEGVVDLIEMKEIVWPVDSD 191 Query: 184 PXXXXXXXXXXXXXXXXXXXT--YMSDTPLDL------------TAWKEQ----LKAGAA 225 T YM ++ ++ KE+ L+ Sbjct: 192 QGQHFDVKDIRAELKEKAEETRQYMLESIVETDDALMEKFFGGEEITKEEIIKGLRKATI 251 Query: 226 AGRWFPLVYGVAAKGLGITQLLDAMTDYFP----------RAGGNPQL----------PV 265 P+V G A K GI LLDA+ +Y P R NP + P Sbjct: 252 DNTIVPVVCGTAFKNKGIQALLDAIVNYMPAPTDVAMVEGRDPKNPDVLIDREMSDDAPF 311 Query: 266 SGIVYSIQRDKTMGRMAYVRLYQGMVRNRDTVMNYTQDVQGKVTQIRKVEGGRTEDVGTL 325 + + + + D +GR+ + R+Y G V TV+N T+ + ++ +I ++ E++ + Sbjct: 312 AALAFKVMTDPFVGRLTFFRVYSGFVEKGATVLNSTKGKKERMGRILQMHANNREEIEHV 371 Query: 326 EAGDIAVVYGLSGVKIGDVLGVPDA--VPQEAKLAVPLLTVRVHWDAAVDEHKVIGAFQE 383 GDIA GL GD L +A V ++ + P+++V V D+ K+ A + Sbjct: 372 YCGDIAAAVGLKDTATGDTLCAENAPIVLEQMEFPEPVISVAVEPKTKNDQEKMGIALSK 431 Query: 384 LADEDPLLDTQWLQDERELHIKVMGPIQLEILDSVLESRYGLKVTFGQPSVIYRET 439 LA+EDP + ++ + I MG + LEI+ ++ + ++ G+P V YRET Sbjct: 432 LAEEDPTFKVRTDEETGQTIISGMGELHLEIIVDRMKREFKVESNVGKPQVAYRET 487 >TM1503 [J] COG0480 Translation elongation factors (GTPases) Length = 695 Score = 246 bits (627), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 148/473 (31%), Positives = 231/473 (48%), Gaps = 44/473 (9%) Query: 11 NVGIFAHVDAGKTTTTEHILYESGRIKAVDSVDSGTALTDSMEVERQRGISVRAALASFE 70 N+GI AH+DAGKTTTTE ILY +GR + VD G TD M E++RGI++++A + Sbjct: 16 NIGIMAHIDAGKTTTTERILYYTGRKHFIGDVDEGNTTTDWMPQEKERGITIQSAATTCF 75 Query: 71 WRGVQVNLVDTPGHVDFLSEVERSLRVMDCAVLILSAVEGVQAQSEMIWNALRKLGIPTL 130 W+G ++N++DTPGHVDF +EVER+LRV+D A+ + A GV+ QSE +W K +P + Sbjct: 76 WKGYRINIIDTPGHVDFTAEVERALRVLDGAIAVFDATAGVEPQSETVWRQADKYNVPRI 135 Query: 131 VFLNKMDRTGADPEAVLVQARTYLSGDILPVQQALGQEQEYAGAVDL------------- 177 F+NKMD+ GAD + T L + +PVQ +G E+++ G +DL Sbjct: 136 AFMNKMDKVGADFYMAVETLVTKLRANPIPVQMPIGSEKDFQGVIDLIKMKAIYWVSEDG 195 Query: 178 --WAEAADPXXXXXXXXXXXXXXXXXXX--------TYMSDTPLDLTAWKEQLKAGAAAG 227 + E P Y+ + K L+ Sbjct: 196 SVYEERDIPEELREEAEMRREEMLEKIAELDEEILEKYLEGEEISEEEIKRVLRKATIEN 255 Query: 228 RWFPLVYGVAAKGLGITQLLDAMTDYFPRAGGNPQL-------------------PVSGI 268 + P++ G A GI LLDA+ DY P P + P + + Sbjct: 256 KAVPVLCGAAKANKGIQPLLDAVIDYLPSPLDLPPVKGWRVSDGEVVYRKPDENEPFTAL 315 Query: 269 VYSIQRDKTMGRMAYVRLYQGMVRNRDTVMNYTQDVQGKVTQIRKVEGGRTEDVGTLEAG 328 V+ +Q D +G++ Y R+Y G + V N T+ + ++++I + + E+V + G Sbjct: 316 VFKVQVDPYIGKLVYFRVYSGRLEKGSYVYNSTKGQRERISRIVFMHADKREEVDYVRPG 375 Query: 329 DIAVVYGLSGVKIGDVLG--VPDAVPQEAKLAVPLLTVRVHWDAAVDEHKVIGAFQELAD 386 DIA GL + GD L + ++ P++++ V DE K++ A L++ Sbjct: 376 DIAAGVGLKVSQTGDTLCDEKEPIILEKIDFPEPVISLAVEPVTKADEEKLVKALLALSE 435 Query: 387 EDPLLDTQWLQDERELHIKVMGPIQLEILDSVLESRYGLKVTFGQPSVIYRET 439 EDP L + ++ E I MG + LEI+ L+ +G+ V GQP V YRET Sbjct: 436 EDPTLQVRVDKETGETIISGMGELHLEIVVDRLKREFGVNVRVGQPQVAYRET 488 >CAC0854 [J] COG0480 Translation elongation factors (GTPases) Length = 644 Score = 244 bits (624), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 143/436 (32%), Positives = 225/436 (51%), Gaps = 28/436 (6%) Query: 12 VGIFAHVDAGKTTTTEHILYESGRIKAVDSVDSGTALTDSMEVERQRGISVRAALASFEW 71 +G+ AHVDAGKTT E ILY + I+ VD + D+ VE++RGI+V + A FE+ Sbjct: 5 IGLLAHVDAGKTTLAEQILYHTNSIRKRGRVDHKDSFLDNSLVEKERGITVFSEQAIFEF 64 Query: 72 RGVQVNLVDTPGHVDFLSEVERSLRVMDCAVLILSAVEGVQAQSEMIWNALRKLGIPTLV 131 +G LVDTPGH+DF E+ER++ +MD AVLI+S V+GVQ+Q+E IW LRK +PT+ Sbjct: 65 KGSTYFLVDTPGHIDFSPEMERAIEIMDYAVLIISGVDGVQSQTENIWRLLRKYNVPTIF 124 Query: 132 FLNKMDRTGADPEAVLVQARTYLSGDILPVQQALGQEQEYAGAVDLWAEAADPXXXXXXX 191 F+NKMDR A E V+ + L+ + ++ G E ++ AE D Sbjct: 125 FINKMDRLNASKEKVIKEIVENLTSKVFLIE---GNEMN-ENTIEFIAENNDD------- 173 Query: 192 XXXXXXXXXXXXTYMSDTPLDLTAWKEQLKAGAAAGRWFPLVYGVAAKGLGITQLLDAMT 251 Y+S + + W +K FP G A + +GI L M Sbjct: 174 ---------LCEIYLS-SGYEENLWFNSMKEMIKKNIIFPCFTGSALEDIGIEYFLQKM- 222 Query: 252 DYFPRAGGNPQLPVSGIVYSIQRDKTMGRMAYVRLYQGMVRNRDTVMNYTQDVQGKVTQI 311 + N G+VY I+ D ++ Y++ +G V+ +D V ++ K+ I Sbjct: 223 NLITYTEYNETEEFLGLVYKIRYDDNKNKIVYIKALKGSVKVKDEVR--VENSSEKIEDI 280 Query: 312 RKVEGGRTEDVGTLEAGDIAVVYGLSGVKIGDVLG-VPDAVPQEAKLAVPLLTVRVHWDA 370 R G + + AG++ V G+ V+ GD +G D + +P L +V +D Sbjct: 281 RFYNGNKYKTEHKAAAGELFAVVGIKNVRSGDFVGSFSDKIKYN---MIPTLKSKVVFDE 337 Query: 371 AVDEHKVIGAFQELADEDPLLDTQWLQDERELHIKVMGPIQLEILDSVLESRYGLKVTFG 430 ++ ++V+ F+ + +E+P L+ W + R L I VMG I+LEIL ++++ R+ L+V FG Sbjct: 338 GINANQVLEYFKIIEEEEPSLNILWNEKLRSLEIHVMGKIELEILKAIMKDRFNLEVDFG 397 Query: 431 QPSVIYRETXXXKPRG 446 V+Y+ET K G Sbjct: 398 PCEVLYKETIAKKTNG 413 >all4338 [J] COG0480 Translation elongation factors (GTPases) Length = 692 Score = 244 bits (624), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 153/484 (31%), Positives = 236/484 (48%), Gaps = 47/484 (9%) Query: 1 MVQQQGMERI-NVGIFAHVDAGKTTTTEHILYESGRIKAVDSVDSGTALTDSMEVERQRG 59 M + +E++ N+GI AH+DAGKTTTTE IL+ SG I + V GTA+TD M+ ER+RG Sbjct: 1 MARINPLEKVRNIGIAAHIDAGKTTTTERILFYSGIIHKIGEVHEGTAVTDWMDQERERG 60 Query: 60 ISVRAALASFEWRGVQVNLVDTPGHVDFLSEVERSLRVMDCAVLILSAVEGVQAQSEMIW 119 I++ AA S W+ Q+N++DTPGHVDF EVERS+RV+D + + +V GVQ QSE +W Sbjct: 61 ITITAAAISTSWKDYQINIIDTPGHVDFTIEVERSMRVLDGVIAVFCSVGGVQPQSETVW 120 Query: 120 NALRKLGIPTLVFLNKMDRTGADPEAVLVQARTYLSGDILPVQQALGQEQEYAGAVDLWA 179 + +P + F+NKMDRTGA+ V Q R L + + +Q +G E ++ G VDL Sbjct: 121 RQADRYKVPRIAFINKMDRTGANFYRVHEQMRDRLRANAIAIQLPIGSENDFKGIVDLVR 180 Query: 180 EAAD-----------------------PXXXXXXXXXXXXXXXXXXXTYMSDTPLDLTAW 216 + A Y PL Sbjct: 181 KRAYMYNNDQGTDIEETDIPADLQDQVEEYYTKLVEAVAETDDDLMSKYFDGEPLTEEEI 240 Query: 217 KEQLKAGAAAGRWFPLVYGVAAKGLGITQLLDAMTDYFPRAGGNPQL------------- 263 + L+ G AG P++ G A K G+ +LDA+ DY P P + Sbjct: 241 RSALRKGTIAGTIVPVLCGSAFKNKGVQLMLDAVVDYLPAPTEVPPIQGTLPNGDAIERR 300 Query: 264 -----PVSGIVYSIQRDKTMGRMAYVRLYQGMVRNRDTVMNYTQDVQGKVTQIRKVEGGR 318 P++ + + I D GR+ +VR+Y G+++ V+N T++ + +++++ ++ Sbjct: 301 ADDNEPLAALAFKIMADP-YGRLTFVRVYSGVLKKGSYVLNATKNKKERISRLVLMKADD 359 Query: 319 TEDVGTLEAGDIAVVYGLSGVKIGDVL---GVPDAVPQEAKLAVPLLTVRVHWDAAVDEH 375 +DV L AGD+ GL GD + G P + + + P+++V V D Sbjct: 360 RQDVEELRAGDLGAALGLKDTLTGDTITDEGAP-VILESLFIPEPVISVAVEPKTKNDMD 418 Query: 376 KVIGAFQELADEDPLLDTQWLQDERELHIKVMGPIQLEILDSVLESRYGLKVTFGQPSVI 435 K+ A Q L++EDP + + I MG + LEIL + + ++ G P V Sbjct: 419 KLSKALQSLSEEDPTFRVNVDPETNQTVIAGMGELHLEILVDRMLREFKVEANVGAPQVA 478 Query: 436 YRET 439 YRET Sbjct: 479 YRET 482 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,003,430 Number of extensions: 1348610 Number of successful extensions: 12388 Number of sequences better than 1.0e-05: 114 Number of HSP's gapped: 12222 Number of HSP's successfully gapped: 149 Length of query: 466 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 356 Effective length of database: 25,691,691 Effective search space: 9146241996 Effective search space used: 9146241996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)