BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0005.p (388 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM2959 [G] COG1929 Glycerate kinase 343 2e-94 ZyhaD [G] COG1929 Glycerate kinase 340 2e-93 ECs4002 [G] COG1929 Glycerate kinase 338 7e-93 yhaD [G] COG1929 Glycerate kinase 338 1e-92 ZyhaD [G] COG1929 Glycerate kinase 338 1e-92 >STM2959 [G] COG1929 Glycerate kinase Length = 380 Score = 343 bits (881), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 181/372 (48%), Positives = 239/372 (64%) Query: 14 FVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHY 73 V+APDS+KES++A EV A+E+G R+++P A Y+ +P+ADGGEGTV+++V+A+ G I + Sbjct: 3 IVIAPDSYKESLSALEVATAIEQGFREIWPDADYLKLPLADGGEGTVEAMVEATAGRIVH 62 Query: 74 KEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECL 133 EV GPLG +V A YG+ G ++A IEMA+ASG+ VP R+PL TT++GTGELIR L Sbjct: 63 VEVTGPLGHRVNAFYGLSGDARSAFIEMAAASGLEQVPPAQRDPLKTTSWGTGELIRHAL 122 Query: 134 DXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDER 193 D SATNDGGAGM +ALGAR D G ++ +GG L+ LASIDIS LD+R Sbjct: 123 DAGVEHIIIGIGGSATNDGGAGMVQALGARLRDAQGNDIAQGGIGLETLASIDISGLDKR 182 Query: 194 LQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVR 253 L + VACDVTNPL G++GAS VFGPQKGATPEM+++LD L YA ++ + LH DV Sbjct: 183 LSACHIEVACDVTNPLTGKEGASAVFGPQKGATPEMIERLDTALTRYAHLIARDLHVDVL 242 Query: 254 DLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFG 313 DL F A L++GIEIV + L+ LA AD+V TGEG ID QT G Sbjct: 243 DLAGGGAAGGMGAALYAFCGAQLRRGIEIVTDALHLEACLADADLVITGEGRIDSQTIHG 302 Query: 314 KTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNV 373 K P GVA AK + VI +AG + + ++E G A+F ++ LE L +NV Sbjct: 303 KVPIGVANIAKRYNKPVIGIAGSLTADVSVVHEHGLDAVFSVIYTICTLEDALKNASENV 362 Query: 374 ERTCENIARILK 385 T N+A LK Sbjct: 363 RMTARNVAATLK 374 >ZyhaD [G] COG1929 Glycerate kinase Length = 301 Score = 340 bits (872), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 0/386 (0%), Positives = 0/386 (0%), Gaps = 5/386 (1%) Query: 1 MVWGEDMKGKEKTFVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTV 60 M + +A + + + + + A + Sbjct: 1 MSIDKSYC-----GFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGA 55 Query: 61 QSLVDASGGEIHYKEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLIT 120 + +H +E A +G + + Sbjct: 56 YQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREG 115 Query: 121 TTYGTGELIRECLDXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLD 180 + + A+ + L+ + Sbjct: 116 KAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPE 175 Query: 181 RLASIDISALDERLQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHY 240 + +R Q + + Sbjct: 176 ATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLI 235 Query: 241 AAVVKQQLHKDVRDLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVF 300 + Q + + + +E+ ++ A Sbjct: 236 LVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSL 295 Query: 301 TGEGGIDFQTKFGKTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGAS 360 Sbjct: 296 355 Query: 361 GLEKLLAEGPQNVERTCENIARILKL 386 Sbjct: 356 381 >ECs4002 [G] COG1929 Glycerate kinase Length = 408 Score = 338 bits (868), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 176/373 (47%), Positives = 239/373 (64%) Query: 14 FVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHY 73 V+APDS+KES++A EV A+EKG R+++P A Y+ VP+ADGGEGTV++++ A+ G + Sbjct: 30 IVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGSERH 89 Query: 74 KEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECL 133 V GPLG+KV A +GI G G+TA IEMA+ASG+ LVP E R+PL+TT+ GTGELI + L Sbjct: 90 AWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQAL 149 Query: 134 DXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDER 193 + SATNDGGAGM +ALGA+ D +G E+ GGGSL+ L IDIS LD R Sbjct: 150 ESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNEIGFGGGSLNTLNDIDISGLDPR 209 Query: 194 LQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVR 253 L+ + VACDVTNPL G+ GASR+FGPQKGA+ M+ +LD NL+HYA V+K+ LH DV+ Sbjct: 210 LKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVDVK 269 Query: 254 DLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFG 313 D+P F A L+ GIEIV L+E + +V TGEG ID Q+ G Sbjct: 270 DVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHG 329 Query: 314 KTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNV 373 K P GVA AK + VI +AG + + + +++ G A+F ++ L++ N+ Sbjct: 330 KVPIGVANVAKKYHKPVIGIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNI 389 Query: 374 ERTCENIARILKL 386 R NIA L + Sbjct: 390 CRASRNIAATLAI 402 >yhaD [G] COG1929 Glycerate kinase Length = 408 Score = 338 bits (867), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 176/373 (47%), Positives = 239/373 (64%) Query: 14 FVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHY 73 V+APDS+KES++A EV A+EKG R+++P A Y+ VP+ADGGEGTV++++ A+ G + Sbjct: 30 IVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADGGEGTVEAMIAATQGAERH 89 Query: 74 KEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECL 133 V GPLG+KV A +GI G G+TA IEMA+ASG+ LVP E R+PL+TT+ GTGELI + L Sbjct: 90 AWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAEKRDPLVTTSRGTGELILQAL 149 Query: 134 DXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDER 193 + SATNDGGAGM +ALGA+ D +G E+ GGGSL+ L IDIS LD R Sbjct: 150 ESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNEIGFGGGSLNTLNDIDISGLDPR 209 Query: 194 LQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVR 253 L+ + VACDVTNPL G+ GASR+FGPQKGA+ M+ +LD NL+HYA V+K+ LH DV+ Sbjct: 210 LKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAMIVELDNNLSHYAEVIKKALHVDVK 269 Query: 254 DLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFG 313 D+P F A L+ GIEIV L+E + +V TGEG ID Q+ G Sbjct: 270 DVPGAGAAGGMGAALMAFLGAELKSGIEIVTTALNLEEHIHDCTLVITGEGRIDSQSIHG 329 Query: 314 KTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNV 373 K P GVA AK + VI +AG + + + +++ G A+F ++ L++ N+ Sbjct: 330 KVPIGVANVAKKYHKPVIGIAGSLTDDVGVVHQHGIDAVFSVLTSIGTLDEAFRGAYDNI 389 Query: 374 ERTCENIARILKL 386 R NIA L + Sbjct: 390 CRASRNIAATLAI 402 Score = 338 bits (867), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 0/373 (0%), Positives = 0/373 (0%) Query: 14 FVLAPDSFKESMTAKEVCIAMEKGLRKVYPAAHYIHVPMADGGEGTVQSLVDASGGEIHY 73 + +++ T + + + + M + AS Sbjct: 30 GQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDV 89 Query: 74 KEVNGPLGQKVTAKYGILGGGQTAAIEMASASGIHLVPKETRNPLITTTYGTGELIRECL 133 + V + + + + ++ V + L Sbjct: 90 ELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFL 149 Query: 134 DXXXXXXXXXXXXSATNDGGAGMAEALGARFLDESGAELPRGGGSLDRLASIDISALDER 193 D F ++ + + + + + + L Sbjct: 150 DIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAE 209 Query: 194 LQHVQMIVACDVTNPLCGEQGASRVFGPQKGATPEMVQKLDANLAHYAAVVKQQLHKDVR 253 L + + + G + + ++ +MV + ++ +++ Sbjct: 210 LPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMF 269 Query: 254 DLPXXXXXXXXXXXXXIFTQAVLQKGIEIVIEYTGLKEKLAGADVVFTGEGGIDFQTKFG 313 Sbjct: 270 329 Query: 314 KTPYGVARAAKAGGQKVIAVAGYIGEGIDALYEEGFTAIFGIVPGASGLEKLLAEGPQNV 373 Sbjct: 330 389 Query: 374 ERTCENIARILKL 386 Sbjct: 390 402 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,143,832 Number of extensions: 1188094 Number of successful extensions: 2796 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 2776 Number of HSP's successfully gapped: 16 Length of query: 388 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 280 Effective length of database: 25,922,359 Effective search space: 7258260520 Effective search space used: 7258260520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 117 (49.7 bits)