BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0238.0004.p (608 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2123 [G] COG0383 Alpha-mannosidase 325 1e-88 BH0791 [G] COG0383 Alpha-mannosidase 322 1e-87 TM1851 [G] COG0383 Alpha-mannosidase 241 3e-63 BH0789 [G] COG0383 Alpha-mannosidase 68 3e-11 >lin2123 [G] COG0383 Alpha-mannosidase Length = 1032 Score = 325 bits (834), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 207/583 (35%), Positives = 304/583 (52%), Gaps = 64/583 (10%) Query: 32 WRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLA----GSSIAPELQRSIAGQ 87 W G+LY E HRGTYT+ K+ NRK E+ R +E LA G++ E + Sbjct: 508 WNGELYFEYHRGTYTSQAFTKKMNRKIELGLRNSEWLSVLANLKEGTTYPTE-------K 560 Query: 88 LHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGE 147 L W+++L NQFHDIIPGS+I E Y+ + EY E + + LE LT Q A E Sbjct: 561 LANIWQILLRNQFHDIIPGSSIKEVYDDALIEYTEALTENNTIISKQLEKLTQQ---ASE 617 Query: 148 GIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXXXXX 207 I L+NS W R P ++++ AD A + Sbjct: 618 KI--TLWNSSTWNR----------------------PRYIEIKADNPASNLVFYDQKN-- 651 Query: 208 XXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELNDSW 267 +R Q I ++P G ++ +++ +T S ++ Sbjct: 652 ---------KRLPAQKLANGAWLIQHHNLPALGATSISVKETTSELTKSPFTYKD--KQL 700 Query: 268 ETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYEAQP 327 ETP+Y++ +NE G+ TS+FDK R+V+ R N F F D+P DAWDID Y+ + Sbjct: 701 ETPYYQITWNENGQFTSIFDKKNNRQVLAENARGNVFQLFEDKPMWHDAWDIDLFYQEKQ 760 Query: 328 AGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAHKLL 387 L V+++G ++ ++ +S+ITQ + Y I F+T VDW + ++LL Sbjct: 761 KEITTLDSFEVINNGPLELTIKQVLHSEKSQITQWIHFYHESPEIRFETEVDWRDRNQLL 820 Query: 388 KVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLLNDC 447 KV FPV V A +A+Y+I FG ++RPTH NTSW+ A++E GH++ D+SE YGVSLLND Sbjct: 821 KVAFPVSVHASEASYDIQFGNVKRPTHWNTSWDYARFETVGHQWTDLSEKNYGVSLLNDS 880 Query: 448 KYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELNHEV 507 KYGYDI+ STIRL+LL+A +PD AD G H FTY+L+PH GD+ T++ A ELN+ Sbjct: 881 KYGYDIKDSTIRLTLLKAAGYPDPEADLGLHQFTYSLFPHQGDFLDGKTVQTAWELNN-- 938 Query: 508 PAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETVKLAWN 567 P ++G+ S + +V++D+VK AE+G G ++RL+E +G V L + Sbjct: 939 PIFTSNGEI-----EPFSLFQVTNPYVMIDSVKQAENGNGLIIRLHEFAGTHGEVTLTSD 993 Query: 568 QPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKT--IYIR 608 + I N LE E E + PFEIKT IY++ Sbjct: 994 YAITSITECNLLE-ENETTLETAT---FAITPFEIKTYRIYLK 1032 >BH0791 [G] COG0383 Alpha-mannosidase Length = 1039 Score = 322 bits (826), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 204/586 (34%), Positives = 296/586 (50%), Gaps = 72/586 (12%) Query: 32 WRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSIAGQ-LHK 90 W G+LYLE HRGTYT+ + K+ NR+ E YR+AE+ LA ++ A + + G L K Sbjct: 510 WDGELYLEYHRGTYTSQAKMKKKNRQLEFSYRDAEI---LAVTASAEKGWDAYPGSSLEK 566 Query: 91 GWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGEGIP 150 GWKL+LLNQFHDIIPGS+I E YE + K+Y E +++Q + + + Sbjct: 567 GWKLLLLNQFHDIIPGSSIKEVYEDAAKDYTEA-----AAIQKQVVTTQEKRMVNDKREK 621 Query: 151 YVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXXXXXXXX 210 + F+ ++ D + V +P+G D+ G L Sbjct: 622 WTFFHP--YQYDTPILVTIPKGTDERFVDQTGHGL------------------------- 654 Query: 211 XXXXSIERATEQATGQVTAYIHVPSIPGFGYKTV-----WLRQVDEAVTGSMPERRELND 265 +++Q T + SIP F Y+T+ +V + ERR Sbjct: 655 --------SSQQTANNETLVLLEKSIP-FAYQTISRATMVAEEVQMLSQVNFEERR---- 701 Query: 266 SWETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYEA 325 WETP Y+L +N+ G+ ++DK RE + G N F F D+P DAWDID Y+ Sbjct: 702 -WETPWYQLEWNDEGQFIRMYDKLGKREWLAQGKLGNVFKRFEDKPLAHDAWDIDLFYQE 760 Query: 326 QPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAHK 385 + + + ++ +G ++ + FRWK I Q + Y H RIDF T VDW + Sbjct: 761 KSSTVSSFQGCELIENGPLRTTVLFRWKEDGVVIKQAIRCYRHTARIDFATEVDWQARQQ 820 Query: 386 LLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLLN 445 LLKV FPVD+ ++A Y+I FG L+RPTH NTSW+ A++E GH++A ++E YG+SLLN Sbjct: 821 LLKVEFPVDIRTNEAIYDIQFGNLKRPTHWNTSWDMARFETVGHKWAAIAEADYGISLLN 880 Query: 446 DCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELNH 505 D KYGY I+ + + LSLL+ +PD AD G H FTYALYP G R+A L Sbjct: 881 DSKYGYSIKDNVLSLSLLKGAIYPDPEADIGNHQFTYALYPFQGPAFKRDVEREATYLQS 940 Query: 506 EVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETVKLA 565 + A+ + ++P V++D +K +EDG ++RL+E G R TVKL Sbjct: 941 AIIAVPGE----AEMPFDQPLFTVSSDQVIVDAIKKSEDGTSVIIRLHEMEGARATVKLD 996 Query: 566 WNQPFSEIYISNALEAE------LEPVIHHEGEFELNFAPFEIKTI 605 + N +E+E PV EL F P+EIKTI Sbjct: 997 CRYEAKQWREVNLIESEALSEWSTRPV-------ELTFTPYEIKTI 1035 >TM1851 [G] COG0383 Alpha-mannosidase Length = 1010 Score = 241 bits (614), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 187/585 (31%), Positives = 287/585 (49%), Gaps = 83/585 (14%) Query: 28 QLPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSIAGQ 87 +LP W G+LYLELHRGTYT+ R K+ +++AE AEL I+ + + + Sbjct: 502 ELPVWDGELYLELHRGTYTSQFRTKKLHKEAEDSLYLAEL--------ISAFTDKDFSDE 553 Query: 88 LHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGE 147 + + WK++L N+FHDI+PGS+I E YE +EKE R V + + L+ L+++ Sbjct: 554 IDELWKILLRNEFHDILPGSSIKEVYEDTEKELRHVIEKSKDIVIESLKVLSSENKDV-- 611 Query: 148 GIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXXXXX 207 + N+ + + + L+ DLA Sbjct: 612 ---LTILNASSFPKKCLFF------------------LNEDLAISFEG------------ 638 Query: 208 XXXXXXXSIERATEQAT--GQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELND 265 E +Q T G+ +I IP F + +R+ T S E RE N Sbjct: 639 ---------EALLKQKTHDGRYVYFID-REIPPFTKVELKVRKATSEETPS--ELRETN- 685 Query: 266 SWETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYEA 325 E R+ N+ G I ++DK R V + G + H + P WD WDI E Sbjct: 686 IMENEFLRVHVNDDGTI-QIYDKELDRYVFEEKGNILKLH--KNIPAYWDNWDIAENVEK 742 Query: 326 QP-AGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAH 384 A+ +EK + SG V++V+R + S ITQ ILY RR+D +T VDW+ Sbjct: 743 TGYTLRAKNIEK--IESGPVREVIRVEHESEGSRITQHYILYRKSRRLDIETKVDWHTRR 800 Query: 385 KLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLL 444 LL+ FP V++ KA ++I G +ERPTHRNTS+EQA++EV HR+ D+S+ +GVS+L Sbjct: 801 ALLRAYFPTTVLSRKARFDISGGFIERPTHRNTSFEQARFEVPFHRWMDLSQTDFGVSIL 860 Query: 445 NDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELN 504 ND KYG + + LSL++A +PD + D+G H FTY++Y H GD +LR + + Sbjct: 861 NDGKYGGSVHQGIMALSLIKAGIFPDFLCDEGEHTFTYSVYVHPGD-----SLRDVVKES 915 Query: 505 HEVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSG--GRETV 562 ++ + LPS + Q+ L +++ +D VLRL E G G+ ++ Sbjct: 916 EDLNRSFIVHRGVLNLPS--PLLEISPQNFRLTSLRRVKD--KIVLRLVEIFGTSGKLSI 971 Query: 563 KLAWNQPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKTIYI 607 KL W+ EIY +N LE + + V F + + PF+I T + Sbjct: 972 KLPWH---GEIYQTNVLEEKKQKVT-----FPVVYHPFKIYTFVV 1008 >BH0789 [G] COG0383 Alpha-mannosidase Length = 777 Score = 68.2 bits (165), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 58/252 (23%), Positives = 109/252 (43%), Gaps = 12/252 (4%) Query: 362 DLILYAHDRRIDFKTHVDWNEAHKLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQ 421 +L L + + ++D ++ FPV+ + + +ERP + W+ Sbjct: 527 ELTLEQGAKGLKVNVNIDNTAKDHRMRALFPVERARGNHYADSIYEIVERPNTPDPKWQN 586 Query: 422 AQYEVCGHRFADVSEHGYGVSLLNDCKYGYDIQGSTIRLSLLRAP----KW----PDKVA 473 ++ R + YG+++ + Y+I +I ++LLR+ W + Sbjct: 587 PAFDHHMQRLVSLDNGEYGLTIATKGLHEYEIVSDSIAVTLLRSVGELGDWGLFETPEAQ 646 Query: 474 DQGAHDFTYALYPHTGDWRSAHTLRKAAELNHEVPAIAADGKTTGQLPSTGSFIRFDGQH 533 G ++ + L PH GD SA+ A + E P + ++ G LP + ++ G+ Sbjct: 647 CFGQNEAQFVLLPHKGDVLSANVYVAAYDDPVE-PTVIQTEQSMGPLPHATNLFQWSGEG 705 Query: 534 VVLDTVKPAEDGRGSVLRLYESSGGRETVKLAWNQPFSEIYISNALEAELEPVIHHEGEF 593 +VL KP DGRG +LR + E + + + F +IY S LE ++E + + Sbjct: 706 LVLTACKPTMDGRGMILRWFNPKREGEAL-IVQSTHFLQIYRSTILEEQIEKLGTENVQI 764 Query: 594 ELNFAPFEIKTI 605 E+ P EI T+ Sbjct: 765 EVR--PQEIVTL 774 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 48,776,480 Number of extensions: 2115096 Number of successful extensions: 4273 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 4263 Number of HSP's successfully gapped: 7 Length of query: 608 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 496 Effective length of database: 25,461,023 Effective search space: 12628667408 Effective search space used: 12628667408 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 119 (50.4 bits)