BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0024.p (702 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ykcB [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and ... 258 3e-68 BS_yycA [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and ... 246 7e-65 CAC0193 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and ... 187 5e-47 CAC2251 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and ... 186 1e-46 >BS_ykcB [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family Length = 716 Score = 258 bits (658), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 138/237 (58%), Positives = 162/237 (68%) Query: 29 WNDQYANTYYTTAVGSMLQSFHNFFFASLDSAGSVTVDKPPVTFWIQTLSALIFGLHGWS 88 W D N YYT AV SM+QSFHNFF+AS D+AG +TVDKPPVT+ IQT+SALIFG+HGWS Sbjct: 28 WKDDTVNPYYTAAVTSMMQSFHNFFYASFDAAGFITVDKPPVTYQIQTISALIFGMHGWS 87 Query: 89 VILPQVIGGMGSVLLVYLLVKPTFGXXXXXXXXXXXXTTPVAGAVSRTNNIDAMLVFTLL 148 VILPQ + G+GSVLL+YLL+KPTFG TP+A AV+RTNN+DA+LVF LL Sbjct: 88 VILPQALAGVGSVLLMYLLIKPTFGKTAARIASFVMACTPIAVAVARTNNVDALLVFFLL 147 Query: 149 LAAWFLFKGTKRNTTGSLLAAFALIGVGFNEKMLQAYMVLPXXXXXXXXXXKVNWKKKTG 208 LA W LFK ++ LLAAF ++GVGFN KMLQAYM+LP KKK Sbjct: 148 LATWLLFKAIRKGKLIWLLAAFFVVGVGFNTKMLQAYMILPAFLLFYLIAANATIKKKIV 207 Query: 209 XXXXXXXXXXXXXXXWAVIVDSIPASKRPYMGSSGTNSVLNLAFGYNGVARLTGDRS 265 W +IVD+IPASKRPY+GSS TNSVL LAFGYNG+ RLTG S Sbjct: 208 SLVSALAVLAAVSLSWPLIVDNIPASKRPYIGSSQTNSVLELAFGYNGIQRLTGQNS 264 Score = 239 bits (609), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 126/285 (44%), Positives = 166/285 (58%), Gaps = 8/285 (2%) Query: 394 PLRLFQSALSGQASWLLPFLLFGCIG--IFASLRRRNFTQEHKEAIFWLAWLVPVMGFFS 451 PLRLFQ LS Q SWLLPF +FG G I + RR + E KE +FW+AWLVP+ GFFS Sbjct: 365 PLRLFQQELSDQISWLLPFAIFGIAGLLIAGARERRRLSVEQKETVFWVAWLVPIAGFFS 424 Query: 452 IAGFFHQYYLIMMAPPIAALAGSGWLRLWTYYKERAVWLSWLLPVATLATAVFQWYIIHP 511 +A FFH YYLIM+AP A L G+GW+ L Y+ + W +WLLP A +AT F+ +I+ Sbjct: 425 VAEFFHHYYLIMLAPRFAPLVGAGWVALVHLYRNQTGWKAWLLPGAIIATTGFELFILRN 484 Query: 512 YDSTVGSGWSIGVLAGGIAVTVLLLALRIFKGQRQRFVHASAXXXXXXXXXXXXYWALTP 571 Y+ +G GWSIGV G+ + +AL +FK +++ F + + YWA TP Sbjct: 485 YNDQIGVGWSIGV---GVIGVLSAIALLLFKQRQKPFSYYVSLAALLALLVMPMYWASTP 541 Query: 572 ITYGSNSMTPAAGPDSRSXXXXXXXXXXXXXXXVNEKLLAYLKEHNTGEKYLFAAMDYGT 631 + YG NS P GP S VNEKL+ YL+E+N+G +YLFA D T Sbjct: 542 LLYGGNSSLPETGPQLAS---MSGKGMGMSDATVNEKLIKYLEENNSGAEYLFATTDSNT 598 Query: 632 AGPYIIDNNEPVVILNGFNNSDVPYTTETLKALVASGKVKYFLVT 676 A PYII + V+ + G++ SD T K LV GKVKYFL + Sbjct: 599 AAPYIIKTKKAVMAIGGYSGSDPAITLTQFKKLVKEGKVKYFLAS 643 >BS_yycA [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family Length = 685 Score = 246 bits (629), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 129/237 (54%), Positives = 152/237 (64%) Query: 29 WNDQYANTYYTTAVGSMLQSFHNFFFASLDSAGSVTVDKPPVTFWIQTLSALIFGLHGWS 88 W D AN YY AV SM QSFHNFF+AS D +G VTVDKPPV WIQT+ ALIFG+H WS Sbjct: 26 WQDDTANQYYLAAVKSMTQSFHNFFYASFDPSGFVTVDKPPVVLWIQTIFALIFGVHTWS 85 Query: 89 VILPQVIGGMGSVLLVYLLVKPTFGXXXXXXXXXXXXTTPVAGAVSRTNNIDAMLVFTLL 148 VI+PQ + G+GSV L+Y +VKPTFG TP+A AVSRTNNID+MLVFTLL Sbjct: 86 VIIPQALAGVGSVFLLYRMVKPTFGVGAARIAALVMALTPIAVAVSRTNNIDSMLVFTLL 145 Query: 149 LAAWFLFKGTKRNTTGSLLAAFALIGVGFNEKMLQAYMVLPXXXXXXXXXXKVNWKKKTG 208 L + L + K+ LL AFALIG+ FN KM+QA+MVLP +V+ KKK G Sbjct: 146 LGSTCLLRAVKQGKLVWLLTAFALIGLAFNMKMMQAFMVLPAFVLFYLIASRVSLKKKIG 205 Query: 209 XXXXXXXXXXXXXXXWAVIVDSIPASKRPYMGSSGTNSVLNLAFGYNGVARLTGDRS 265 WA+ VDS +S RPY+GSS TNSVL LAFGYNG RL G + Sbjct: 206 SLLLSLVLLTGLSLSWAIAVDSASSSSRPYVGSSQTNSVLELAFGYNGTERLFGQTT 262 Score = 234 bits (597), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 135/285 (47%), Positives = 168/285 (58%), Gaps = 10/285 (3%) Query: 394 PLRLFQSALSGQASWLLPFLLFGCIGIFAS--LRRRNFTQEHKEAIFWLAWLVPVMGFFS 451 PLRLFQSALSGQ SW+LPF L G +G S RR + KE +FW AWLVPV GFFS Sbjct: 355 PLRLFQSALSGQISWMLPFSLIGLLGAIISWYRDRRGHAAKMKETLFWAAWLVPVAGFFS 414 Query: 452 IAGFFHQYYLIMMAPPIAALAGSGWLRLWTYYKERAVWLSWLLPVATLATAVFQWYIIHP 511 IAGFFHQYYLIM+APPIAAL+G GW + YK W S+LLP A L TAVFQ YI+ Sbjct: 415 IAGFFHQYYLIMLAPPIAALSGIGWYTMHRLYKNNKDWSSYLLPAAVLITAVFQVYILSA 474 Query: 512 YDSTVGSGWSIGVLAGGIAVTVLLLALRIFKGQRQRFVHASAXXXXXXXXXXXXYWALTP 571 Y S +GS W + G+ +T+ LL L+ + F YW+ TP Sbjct: 475 YTSQIGSVWMYVLGLLGLGITLALLMLK----RSHPFSKLLTIISLCVLLLTPVYWSATP 530 Query: 572 ITYGSNSMTPAAGPDSRSXXXXXXXXXXXXXXXVNEKLLAYLKEHNTGEKYLFAAMDYGT 631 + YG NS+ P +GP + V+ LL+YL++HNTGE+YLFA + T Sbjct: 531 LLYGGNSVLPESGPQLKG----SANGGNMFSSEVDSGLLSYLRKHNTGEEYLFATLTPVT 586 Query: 632 AGPYIIDNNEPVVILNGFNNSDVPYTTETLKALVASGKVKYFLVT 676 A PYII NE V+ + GFN +D T LK L+ GKVKYFL++ Sbjct: 587 AAPYIIYENESVMAMGGFNGTDPILTVSELKKLIKEGKVKYFLLS 631 >CAC0193 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family Length = 667 Score = 187 bits (475), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 101/230 (43%), Positives = 136/230 (59%) Query: 31 DQYANTYYTTAVGSMLQSFHNFFFASLDSAGSVTVDKPPVTFWIQTLSALIFGLHGWSVI 90 + YANT+Y V SM+ +F NFFFAS D +G VT+DKPPV FWIQT+ A +FG GWS+I Sbjct: 32 EGYANTFYAAGVKSMMMNFKNFFFASYDPSGFVTIDKPPVGFWIQTIFAKVFGFKGWSII 91 Query: 91 LPQVIGGMGSVLLVYLLVKPTFGXXXXXXXXXXXXTTPVAGAVSRTNNIDAMLVFTLLLA 150 LPQ I G+ SVL++Y LVK +FG TP+ A SR N ID +LV +L+L+ Sbjct: 92 LPQAIAGVLSVLIIYYLVKRSFGKPAGLIAALCLAITPIFVAASRNNTIDNLLVLSLVLS 151 Query: 151 AWFLFKGTKRNTTGSLLAAFALIGVGFNEKMLQAYMVLPXXXXXXXXXXKVNWKKKTGXX 210 AW L + LL + A++G+GFN KM++AYMV P + +KK+ Sbjct: 152 AWALSIAAENGKLKYLLISLAVVGIGFNIKMVEAYMVAPAIYITYLLSSTLPFKKRIKHL 211 Query: 211 XXXXXXXXXXXXXWAVIVDSIPASKRPYMGSSGTNSVLNLAFGYNGVARL 260 WAV+ D +PA RPY+GSS N+VL L G+NG+ R+ Sbjct: 212 ALGTIILLLVSLSWAVVTDFVPAKNRPYIGSSNNNTVLQLIIGHNGLQRI 261 Score = 102 bits (254), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 90/300 (30%), Positives = 147/300 (49%), Gaps = 30/300 (10%) Query: 395 LRLFQ-SALSGQASWLLPFLLFGCIGIFASLRRR---NFTQEHKEAI-FWLAWLVPVMGF 449 L+LF + +S Q SWLLPF L G + AS++ F + K A+ FW WL+P + Sbjct: 309 LKLFSGNNMSDQISWLLPFALIGFV--IASVKENFKFPFNNKRKLALLFWFTWLLPEFIY 366 Query: 450 FSIA-GFFHQYYLIMMAPPIAALAGSGWLRLWTYYKERAVWLSWLLPVATLATAVFQWYI 508 FS + H YYL MAP IAAL G G + + +K+ W W+LPVA + A+ + I Sbjct: 367 FSFSRNVTHTYYLTTMAPSIAALTGIGLVTMLKLFKDLPRW-KWILPVAFIVNALTEIII 425 Query: 509 I-HPYDSTVG-SGWSIGVLAGGIAVTVLLLALRIFKGQRQRFVHASAXXXXXXXXXXX-X 565 + + ++S+ G + +L GI V+L+ + I +G+ ++ + Sbjct: 426 LGYKFNSSTGYKIVILMILLLGILPMVILVLIMISQGKNNMILNRNLIYTGFAGLLIAPV 485 Query: 566 YWALTPITYGSNSMTPAAG-----PDSRSXXXXXXXXXXXXXXXVNEKLLAYLKEHNTGE 620 W+ TPI + N +P+AG +SR+ +L+ +L + E Sbjct: 486 IWSATPIFHKMNGSSPSAGLELFNSNSRNYANISSANI--------SRLINFLNTNRKNE 537 Query: 621 KYLF---AAMDYGTAGPYIIDNNEPVVILNGFNNSDVPYTTETLKALVASGKVKYFLVTT 677 KYL +A YG+ I+ +P++ L GF+ SD T + K LV +G ++Y ++TT Sbjct: 538 KYLVEVPSATIYGS--DLILKTGKPILTLGGFSGSDPILTLKKFKQLVKNGSLRYAMITT 595 >CAC2251 [M] COG1807 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family Length = 723 Score = 186 bits (472), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 99/231 (42%), Positives = 130/231 (56%) Query: 31 DQYANTYYTTAVGSMLQSFHNFFFASLDSAGSVTVDKPPVTFWIQTLSALIFGLHGWSVI 90 + Y N YY +AV SM S NFFF S D AG VT+DKPPV FW+Q +SA IFG G S++ Sbjct: 31 EGYGNAYYASAVKSMTMSLKNFFFVSFDPAGFVTIDKPPVGFWLQAISAKIFGFSGVSIL 90 Query: 91 LPQVIGGMGSVLLVYLLVKPTFGXXXXXXXXXXXXTTPVAGAVSRTNNIDAMLVFTLLLA 150 LP G+ SV+L+Y +VK FG TP+ AVSR N ID +L+ LLLA Sbjct: 91 LPSAAAGVISVILIYKIVKRQFGSISALLSALFLSITPIFVAVSRNNTIDNILIMFLLLA 150 Query: 151 AWFLFKGTKRNTTGSLLAAFALIGVGFNEKMLQAYMVLPXXXXXXXXXXKVNWKKKTGXX 210 W L ++ L+ + AL+GVGFN KM++AY+V+P V+ KK+ Sbjct: 151 LWALTIAAEKGKIKYLIISLALVGVGFNVKMVEAYLVIPAIYITYLLTTAVSIKKRLIHL 210 Query: 211 XXXXXXXXXXXXXWAVIVDSIPASKRPYMGSSGTNSVLNLAFGYNGVARLT 261 WA IVD +PAS RPY+ SS N+V+ L G+NG RL+ Sbjct: 211 VCGTLVLVVVSLSWAFIVDLVPASSRPYVDSSTNNTVMELILGHNGTERLS 261 Score = 94.4 bits (233), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 83/296 (28%), Positives = 129/296 (43%), Gaps = 20/296 (6%) Query: 396 RLF-QSALSGQASWLLPFLLFGCIG-IFASLRRRNFTQEHKEAI-FWLAWLVPVMGFFSI 452 RLF + L+ Q W +P +FG I + R + + K+AI W W +P +FS Sbjct: 353 RLFLGNTLADQVVWFIPLAIFGFIASVLKEKLRLSLDNKKKQAIVMWFMWFLPEFIYFSF 412 Query: 453 -AGFFHQYYLIMMAPPIAALAGSGWLRLWTYYKERAVWLSWLLPVATLATAVFQWYIIHP 511 G +H YYL M APP AALAG G LW YKE + ++LLP A L + Q ++ Sbjct: 413 NTGTWHPYYLTMFAPPTAALAGIGITSLWEIYKEGG-FKAYLLPAALLISGASQIIMLSY 471 Query: 512 YDSTVGSGWSIGVLAGGIAVTVL-LLALRIFK------GQRQRFVHASAXXXXXXXXXXX 564 + +++ + +L I + +L +AL IF G + + Sbjct: 472 FMTSLDIAKVLMILV--IILNILPAVALVIFNITGKTIGDLKNIKRNAVSLALVGIIITP 529 Query: 565 XYWALTPITYGSNSMTPAAG-----PDSRSXXXXXXXXXXXXXXXVNEKLLAYLKEHN-T 618 + + N+ P AG +S+S N KL+ +LKE+ T Sbjct: 530 LVGSGAVLINKVNNSMPVAGLELLSSNSKSKIGGGMGVMPDKENSSNLKLINFLKENKTT 589 Query: 619 GEKYLFAAMDYGTAGPYIIDNNEPVVILNGFNNSDVPYTTETLKALVASGKVKYFL 674 +KYL + +A I+ E V+ L GF SD T K +V G+++Y + Sbjct: 590 SQKYLLVVENSNSASEIIVSTGESVMSLGGFLGSDKILTLAQFKGMVKKGEIRYVM 645 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.137 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,713,779 Number of extensions: 1307706 Number of successful extensions: 6839 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 6824 Number of HSP's successfully gapped: 8 Length of query: 702 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 589 Effective length of database: 25,345,689 Effective search space: 14928610821 Effective search space used: 14928610821 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 119 (50.4 bits)