BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0021.p (98 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yczJ [R] COG2329 Uncharacterized enzyme involved in biosynthe... 127 5e-30 DR1636 [R] COG2329 Uncharacterized enzyme involved in biosynthes... 120 7e-28 AGpA263 [R] COG2329 Uncharacterized enzyme involved in biosynthe... 67 9e-12 >BS_yczJ [R] COG2329 Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides Length = 95 Score = 127 bits (318), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 55/95 (57%), Positives = 73/95 (76%) Query: 1 MILEAAMLQIRPGLTSQFEQSFKAASVLISSIEGYLGHELQHCLEDDHKYLLLVKWRNLE 60 M+ EAAML I+ GL +FE +F+ A+ +IS ++GY+ H L C+E+ HKYLLLV+W LE Sbjct: 1 MVREAAMLHIKEGLEQEFEDAFRQAAPIISGMKGYITHSLSKCMEETHKYLLLVEWETLE 60 Query: 61 DHTIGFRESSQYQEWKAMLHHYYSPFPVVEHFTRI 95 DHT GFR SS+YQEWKA+LH +Y+PFP VEHF + Sbjct: 61 DHTEGFRGSSEYQEWKALLHRFYTPFPTVEHFQDV 95 >DR1636 [R] COG2329 Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides Length = 211 Score = 120 bits (300), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 3/96 (3%), Positives = 21/96 (21%) Query: 2 ILEAAMLQIRPGLTSQFEQSFKAASVLISSIEGYLGHELQHCLEDDHKYLLLVKWRNLED 61 + ++ ++ + + + + + + + +K L + Sbjct: 24 LNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYN 83 Query: 62 HTIGFRESSQYQEWKAMLHHYYSPFPVVEHFTRINL 97 + + +E + Sbjct: 84 SKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGR 119 >AGpA263 [R] COG2329 Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides Length = 444 Score = 66.6 bits (161), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 4/96 (4%), Positives = 11/96 (11%) Query: 1 MILEAAMLQIRPGLTSQFEQSFKAASVLISSIEGYLGHELQHCLEDDHKYLLLVKWRNLE 60 M + A + S + S Sbjct: 1 MKIAALRPEQPTPSGVDHRISIFEGDLGALSRSSGHAGLSVSNELFKWAGRSPSTLAWDF 60 Query: 61 DHTIGFRESSQYQEWKAMLHHYYSPFPVVEHFTRIN 96 + + + + Sbjct: 61 TALALGVVGADRLVNRLQTSADGWTREIDLTVAVSD 96 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 6,785,261 Number of extensions: 202587 Number of successful extensions: 340 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 340 Number of HSP's successfully gapped: 3 Length of query: 98 Length of database: 38,378,431 Length adjustment: 67 Effective length of query: 31 Effective length of database: 30,651,053 Effective search space: 950182643 Effective search space used: 950182643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 109 (46.6 bits)