BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0006.p (211 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydgI [C] COG0778 Nitroreductase 338 5e-93 lin0151 [C] COG0778 Nitroreductase 264 8e-71 BH3859 [C] COG0778 Nitroreductase 242 4e-64 L102062_2 [C] COG0778 Nitroreductase 228 6e-60 BS_yodC [C] COG0778 Nitroreductase 164 1e-40 >BS_ydgI [C] COG0778 Nitroreductase Length = 346 Score = 338 bits (866), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 8/207 (3%), Positives = 29/207 (14%) Query: 5 QTNDFTSIITGRRSIRKYDPSVKISKAEMTEILTEATLAPSSVNMQPWRFLVIESAEGKA 64 S + + + + +A + + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 65 KLAPLAKFNQQQVETSAAVIAVFVDMNNFDYAEEIYGTAVERGLMPAEVKERQLAALGAH 124 L N S + + ++ + + + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 125 FANLPAAVNRETIMIDGGLASMQLMLAARAHGYDTNPIGGFEKDQIAEVFGMDKERYIPV 184 L + A + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 185 MLISIGKAAEAGYASVRLPIDTIAQWK 211 + A + + + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYS 209 >lin0151 [C] COG0778 Nitroreductase Length = 346 Score = 264 bits (674), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 12/208 (5%), Positives = 36/208 (17%) Query: 3 TTQTNDFTSIITGRRSIRKYDPSVKISKAEMTEILTEATLAPSSVNMQPWRFLVIESAEG 62 +S+ + + S+A+ L A ++ + V E Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 63 KAKLAPLAKFNQQQVETSAAVIAVFVDMNNFDYAEEIYGTAVERGLMPAEVKERQLAALG 122 + + + +I ++ Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 123 AHFANLPAAVNRETIMIDGGLASMQLMLAARAHGYDTNPIGGFEKDQIAEVFGMDKERYI 182 V+ + + + + + Q A Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 183 PVMLISIGKAAEAGYASVRLPIDTIAQW 210 + + R+ Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYS 209 >BH3859 [C] COG0778 Nitroreductase Length = 346 Score = 242 bits (617), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 12/207 (5%), Positives = 41/207 (19%) Query: 5 QTNDFTSIITGRRSIRKYDPSVKISKAEMTEILTEATLAPSSVNMQPWRFLVIESAEGKA 64 + + T + + A A + + +G Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 65 KLAPLAKFNQQQVETSAAVIAVFVDMNNFDYAEEIYGTAVERGLMPAEVKERQLAALGAH 124 + + + + + + A++R + + + + Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 125 FANLPAAVNRETIMIDGGLASMQLMLAARAHGYDTNPIGGFEKDQIAEVFGMDKERYIPV 184 + G L A DT + + Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 185 MLISIGKAAEAGYASVRLPIDTIAQWK 211 ++ + A + + + + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLN 212 >L102062_2 [C] COG0778 Nitroreductase Length = 346 Score = 228 bits (580), Expect = 6e-60, Method: Compositional matrix adjust. Identities = 10/196 (5%), Positives = 36/196 (18%) Query: 16 RRSIRKYDPSVKISKAEMTEILTEATLAPSSVNMQPWRFLVIESAEGKAKLAPLAKFNQQ 75 + R S+++S + + + + + Q + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 76 QVETSAAVIAVFVDMNNFDYAEEIYGTAVERGLMPAEVKERQLAALGAHFANLPAAVNRE 135 + + ++ + I + A + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 136 TIMIDGGLASMQLMLAARAHGYDTNPIGGFEKDQIAEVFGMDKERYIPVMLISIGKAAEA 195 L + ++ + +D Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 196 GYASVRLPIDTIAQWK 211 A + +A Sbjct: 181 ETAFGVMRYLHLADKH 196 >BS_yodC [C] COG0778 Nitroreductase Length = 346 Score = 164 bits (415), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 10/199 (5%), Positives = 33/199 (16%), Gaps = 4/199 (2%) Query: 12 IITGRRSIRKYDPSVKISKAEMTEILTEATLAPSSVNMQPWRFLVIESAEGKAKLAPLAK 71 + ++ + + + + + + L Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT- 65 Query: 72 FNQQQVETSAAVIAVFVDMNNFDYAEEIYGTAVERGLMPAEVKERQLAALGAHFANLPAA 131 + + + + R + K + A + Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 132 VNRETIMIDGGLASMQLMLAARAHGYDTNPIGGFEKDQIAEVFGMDKERYIPVMLISIGK 191 + + D+ P A I + +S Sbjct: 126 --LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQR-AGKIRYIGVSNET 182 Query: 192 AAEAGYASVRLPIDTIAQW 210 A + + Sbjct: 183 AFGVMRYLHLADKHDLPRI 201 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.131 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,984,556 Number of extensions: 522120 Number of successful extensions: 11423 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 11408 Number of HSP's successfully gapped: 45 Length of query: 211 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 109 Effective length of database: 26,614,363 Effective search space: 2900965567 Effective search space used: 2900965567 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)