BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0003.p (259 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ykrU [R] COG0388 Predicted amidohydrolase 308 7e-84 lin0310 [R] COG0388 Predicted amidohydrolase 286 3e-77 BH1047 [R] COG0388 Predicted amidohydrolase 214 1e-55 PA3797 [R] COG0388 Predicted amidohydrolase 151 8e-37 AF0115 [R] COG0388 Predicted amidohydrolase 145 6e-35 >BS_ykrU [R] COG0388 Predicted amidohydrolase Length = 259 Score = 308 bits (788), Expect = 7e-84, Method: Compositional matrix adjust. Identities = 143/256 (55%), Positives = 177/256 (69%), Gaps = 1/256 (0%) Query: 3 ISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEGNA 62 IS LQ DI++G P N D ++LPELWTTGYDL L E+AD +G + Sbjct: 5 ISCLQFDISYGKPSENIKKAEFFIEKESKHA-DVLVLPELWTTGYDLANLDELADEDGRS 63 Query: 63 TKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEHLY 122 ++ + A++ G+HIVAGSVA R +S + N+MYI + G++ EY K HLF+LMDEHLY Sbjct: 64 AQSWLKKTAKKHGVHIVAGSVAVRKNSDVYNTMYIADKEGQIIKEYRKAHLFQLMDEHLY 123 Query: 123 LQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRALL 182 L G+ G F LDG +GLICYDIRFPEW+R HT GA +LFISAEWPLPRL HW++LL Sbjct: 124 LSAGSEDGYFELDGVKSSGLICYDIRFPEWIRKHTTKGANVLFISAEWPLPRLDHWKSLL 183 Query: 183 ISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKVRE 242 I+RAIENQC+V ACN G +P N FAGHS+IIDPWG V+ E E I+R EIDLQ+ E Sbjct: 184 IARAIENQCFVAACNCTGSNPDNEFAGHSLIIDPWGRVLAEGGREEGIVRAEIDLQESAE 243 Query: 243 VRAQIPVFSDRRPELY 258 VR IPVF D R +LY Sbjct: 244 VRESIPVFDDIRKDLY 259 >lin0310 [R] COG0388 Predicted amidohydrolase Length = 259 Score = 286 bits (731), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 131/257 (50%), Positives = 167/257 (64%) Query: 2 KISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEGN 61 K++L Q D+ F P NY G D +LPE+W TGY L LA +AD G Sbjct: 3 KLALCQTDVVFKYPDANYARIEKAIVEAAENGADIAVLPEMWNTGYALNELAGVADLNGE 62 Query: 62 ATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEHL 121 TK ++GL+ + I I+ GSV+ S +N+MY F + G L Y K+HLF+LM+EHL Sbjct: 63 RTKEFLAGLSEKHQIAIIGGSVSISEGSKFSNTMYAFDKYGGLLSSYKKVHLFQLMNEHL 122 Query: 122 YLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRAL 181 YL+PG K LF LDG SCAG ICYDIRFPEW+R HT+ G+E++F+SA+WP R+ W L Sbjct: 123 YLEPGNDKNLFRLDGISCAGFICYDIRFPEWIRKHTSEGSEVIFVSAQWPAERIKQWEQL 182 Query: 182 LISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEASGGEEILRGEIDLQKVR 241 LI+RAIENQ +V+A NR G+DP+N F GHS++IDP G VI E + EIDL V Sbjct: 183 LIARAIENQAFVVAVNRVGDDPNNHFNGHSLVIDPLGNVIVHGGEEEGNIYAEIDLNLVA 242 Query: 242 EVRAQIPVFSDRRPELY 258 E R IPVF+DRRPELY Sbjct: 243 ETRGIIPVFTDRRPELY 259 >BH1047 [R] COG0388 Predicted amidohydrolase Length = 271 Score = 214 bits (544), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 111/263 (42%), Positives = 154/263 (58%), Gaps = 4/263 (1%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGT--DCVILPELWTTGYDLTRLAEIADP 58 MK++L Q+DI G+P+ N Q D ++LPE+WTT Y L +L +A+ Sbjct: 1 MKVALYQMDILPGDPRGNERKVKEWIEDVMQQEDVPDLLVLPEMWTTAYTLDQLEHLAEG 60 Query: 59 EGNATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMD 118 E T+ + LARE ++IVAGS+A + + N +F R G +Y K+HL ++ Sbjct: 61 EERYTELFLKELAREHNVNIVAGSIAKKEKGKLYNRALVFDRRGHTVYQYDKIHLVPMLS 120 Query: 119 EHLYLQPG-AAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAH 177 E YL G AA +F L+G +ICYD+RFPE +R+ GAEI+FI AEWP R H Sbjct: 121 EPDYLTGGDAAASVFELEGTKMGLVICYDLRFPELMRSLALEGAEIVFIVAEWPEARAVH 180 Query: 178 WRALLISRAIENQCYVIACNRAGEDPSNTFAGHSMIIDPWGEVICEAS-GGEEILRGEID 236 W L +RAIENQ YVI+CNR G TFAG SM+IDPWG+V+ + S E+ L +++ Sbjct: 181 WEVLQRARAIENQSYVISCNRVGAYAGVTFAGRSMVIDPWGDVLIQGSRDQEQTLTTKLN 240 Query: 237 LQKVREVRAQIPVFSDRRPELYQ 259 KV EVR +P+F R P Y+ Sbjct: 241 PDKVSEVREAVPIFQSRVPTKYK 263 >PA3797 [R] COG0388 Predicted amidohydrolase Length = 264 Score = 151 bits (382), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 89/253 (35%), Positives = 132/253 (52%), Gaps = 7/253 (2%) Query: 1 MKISLLQLDIAFGNPQVNYXXXXXXXXXXXXQGTDCVILPELWTTGYDLTRLAEIADPEG 60 + I+L+Q + + + Q N G D V+LPE++TTG+ + AE A+PE Sbjct: 10 LDIALVQSSLVWHDAQAN--REHFAALLESAAGADLVVLPEMFTTGFSMAS-AEQAEPEL 66 Query: 61 NATKALMSGLARELGIHIVAGSVASRNDSGINNSMYIFSRTGELAGEYSKLHLFRLMDEH 120 T A + AR LG +V GS+ + G + + +++R Y K HLFR+ EH Sbjct: 67 GPTHAWLLEQARRLGA-VVTGSLIVQLADGSHRNRLLWARPDGEMLHYDKRHLFRMAGEH 125 Query: 121 LYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVRAHTASGAEILFISAEWPLPRLAHWRA 180 + PG + LF L G LICYD+RFP W R ++L +A WP PR HW Sbjct: 126 EHYSPGERQELFELKGWRVRPLICYDLRFPVWSR--DPHDTDLLLYTANWPAPRRQHWNR 183 Query: 181 LLISRAIENQCYVIACNRAGEDPSN-TFAGHSMIIDPWGEVICEASGGEEILRGEIDLQK 239 LL +RAIEN CYV+A NR GED + +AG S ++D G+ + A+ + + R +D Sbjct: 184 LLPARAIENLCYVVAVNRIGEDGNALRYAGDSQVLDFQGDSLFNAADADGVFRVRLDAAA 243 Query: 240 VREVRAQIPVFSD 252 + R + P + D Sbjct: 244 LAAYRQRFPAYMD 256 >AF0115 [R] COG0388 Predicted amidohydrolase Length = 257 Score = 145 bits (366), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 86/223 (38%), Positives = 120/223 (53%), Gaps = 6/223 (2%) Query: 35 DCVILPELWTTGYDLTRLAEIADPEGNATKALMSGLARELGIHIVAGSVASRNDSGINNS 94 D VILPE++ TG+ + +L+ ++ + I I+ G VA R + NS Sbjct: 40 DMVILPEVFNTGFYKHNYETVE--PLEEELSLLLKISEQKDIMIITG-VAEREGDDLYNS 96 Query: 95 MYIFSRTGELAGEYSKLHLFRLMDEHLYLQPGAAKGLFTLDGASCAGLICYDIRFPEWVR 154 I + G++ G+Y K HLF L +E Y + G +F LICY++RFPE R Sbjct: 97 AVIIHK-GKIIGKYRKTHLFPLTNEKKYFKAGDKLEVFETHLGKIGLLICYEVRFPELSR 155 Query: 155 AHTASGAEILFISAEWPLPRLAHWRALLISRAIENQCYVIACNRAGEDPSNTFAGHSMII 214 GAEI+ I AE+P R+ HWR LL +RAIENQ +V N D + GHSM+I Sbjct: 156 KLVKMGAEIIVIPAEFPKERIDHWRVLLQARAIENQVFVAGVNCVEGDLD--YGGHSMLI 213 Query: 215 DPWGEVICEASGGEEILRGEIDLQKVREVRAQIPVFSDRRPEL 257 DP G V+ EAS +E++ +I L V EVR + P +D R EL Sbjct: 214 DPMGTVLIEASEYQEVMMSDIRLGDVYEVRKKFPFLNDLREEL 256 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,571,715 Number of extensions: 747440 Number of successful extensions: 1393 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 1227 Number of HSP's successfully gapped: 84 Length of query: 259 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 155 Effective length of database: 26,383,695 Effective search space: 4089472725 Effective search space used: 4089472725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)