BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0002.p (345 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3822 [H] COG0095 Lipoate-protein ligase A 167 3e-41 lin2711 [H] COG0095 Lipoate-protein ligase A 155 6e-38 BS_ywfL [H] COG0095 Lipoate-protein ligase A 153 3e-37 SA0546 [H] COG0095 Lipoate-protein ligase A 131 2e-30 SPy1220 [H] COG0095 Lipoate-protein ligase A 127 4e-29 >BH3822 [H] COG0095 Lipoate-protein ligase A Length = 278 Score = 167 bits (422), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 87/208 (41%), Positives = 126/208 (60%), Gaps = 6/208 (2%) Query: 83 PGGDMLLPFAFEETLCRDVGTGLVPPSVHLWSHPGGVALGLRDSKLPRAAEAMAELEGKG 142 P L FA+++TLC +G PP++ W H V LG++DS+LP+ + L+G Sbjct: 23 PTFQALQSFAYDDTLCTSIGKSQSPPTLRAWVHHNTVVLGIQDSRLPQIKAGIEALKGFQ 82 Query: 143 YRTAVRHSGGAAVPLDAGVVNVSLLLRKSPGKLDFHDDFRLLASLITEAVAVSHPQAAAQ 202 + VR+SGG AV LD+G++N+SL+L++ G D + L+ LI ++ H + Q Sbjct: 83 HDVIVRNSGGLAVVLDSGILNLSLVLKEEKG-FSIDDGYELMYELIC-SMFQDHRE---Q 137 Query: 203 IRAGEVAGSYCPGDFDLAIGGRKFCGIAQRRQSSAYFVHAFVIVSGSGLERGQLIRGFYD 262 I A E+ GSYCPG +DL+I G+KF GI+QRR V ++ VSGSG ER ++IR FYD Sbjct: 138 IEAREIVGSYCPGSYDLSIDGKKFAGISQRRIRGGVAVQIYLCVSGSGAERAKMIRTFYD 197 Query: 263 TAADGD-DALAYPRVIPQTLAALGELGG 289 A G YPR+ P+T+A+L EL G Sbjct: 198 KAVAGQPTKFVYPRIKPETMASLSELLG 225 >lin2711 [H] COG0095 Lipoate-protein ligase A Length = 671 Score = 155 bits (393), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 17/211 (8%), Positives = 31/211 (14%), Gaps = 5/211 (2%) Query: 78 NPAAEPGGDMLLPFAFEETLCRDVGTGLVPPSVHLWSHPGGVALGLRDSKLPRAAEAMAE 137 + D E L L + + Sbjct: 18 HEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEV 77 Query: 138 LEGKGYRTAVRHSGGAAVPLDAGVVNVSLLLRKSPGKLDFHDDFRLLASLITEAVAVSHP 197 S A + + + +L A Sbjct: 78 PMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLAVSILYENGVLVSA---- 133 Query: 198 QAAAQIRAGEVAGSYCPGDFDLAIGGRKFCGIAQRRQSSAYFVHAFVIVSGSGLERGQLI 257 GE S + + A+ F+ + R Sbjct: 134 ATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAGFEKINEDARRTGG 193 Query: 258 RGFYDTA-ADGDDALAYPRVIPQTLAALGEL 287 + F + A I Sbjct: 194 KVFANPRNAAAGSLRQLDPRITAKRPLTFFC 224 >BS_ywfL [H] COG0095 Lipoate-protein ligase A Length = 281 Score = 153 bits (387), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 85/229 (37%), Positives = 120/229 (52%), Gaps = 8/229 (3%) Query: 91 FAFEETLCRDVGTGLVPPSVHLWSHPGGVALGLRDSKLPRAAEAMAELEGKGYRTAVRHS 150 FA ++TLC VG G+ P + W H + LG++D++LP + ++ LE +GYR VR+S Sbjct: 32 FAMDDTLCMSVGKGVSPATARSWVHHDTIVLGIQDTRLPFLQDGISLLESEGYRVIVRNS 91 Query: 151 GGAAVPLDAGVVNVSLLLRKSPGKLDFHDDFRLLASLITEAVAVSHPQAAAQIRAGEVAG 210 GG AV LD GV+N+SL+ +D + + L+ + + A+I A E+ G Sbjct: 92 GGLAVVLDDGVLNISLIFEDEKKGIDIDKGYEAMVELMRRMLRPYN----AKIEAYEIEG 147 Query: 211 SYCPGDFDLAIGGRKFCGIAQRRQSSAYFVHAFVIVSGSGLERGQLIRGFYDTA---ADG 267 SYCPG +DL+I G+KF GI+QRR V ++ SG ER LIR FY A Sbjct: 148 SYCPGSYDLSINGKKFAGISQRRVRGGVAVQIYLCADKSGSERADLIRRFYQAALKDKQN 207 Query: 268 DDALAYPRVIPQTLAALGE-LGGPASAAVFAEGFRLALTRRGVHLQSAG 315 D YP + P+T+A+L E L S L HL SAG Sbjct: 208 DKKGVYPEIRPETMASLSELLQKDISVQDLMFALLTELKALSTHLYSAG 256 >SA0546 [H] COG0095 Lipoate-protein ligase A Length = 278 Score = 131 bits (329), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 73/201 (36%), Positives = 108/201 (53%), Gaps = 9/201 (4%) Query: 90 PFAFEETLCRDVGTGLVPPSVHLWSHPGGVALGLRDSKLPRAAEAMAELEGK-GYRTAVR 148 FAF++T C VG + V W H V LG+ DS+LP + + L + GY VR Sbjct: 27 SFAFDDTFCESVGKDISDNVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVR 86 Query: 149 HSGGAAVPLDAGVVNVSLLLR-KSPGKLDFHDDFRLLASLITEAVAVSHPQAAAQIRAGE 207 +SGG V LD GV+N+SL+ + ++ +D + F ++ LI++ I E Sbjct: 87 NSGGLGVVLDQGVLNISLMFKGQTETTID--EAFTVMYLLISKMF----EDENVDIDTME 140 Query: 208 VAGSYCPGDFDLAIGGRKFCGIAQRRQSSAYFVHAFVIVSGSGLERGQLIRGFYDTAADG 267 + SYCPG FDL+I G+KF GI+QRR V ++ V GSG ER +++ FY+ A G Sbjct: 141 IEHSYCPGKFDLSIDGKKFAGISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKG 200 Query: 268 D-DALAYPRVIPQTLAALGEL 287 + YP + P +A+L L Sbjct: 201 EVTKFKYPEIEPSCMASLETL 221 >SPy1220 [H] COG0095 Lipoate-protein ligase A Length = 283 Score = 127 bits (318), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 76/227 (33%), Positives = 112/227 (49%), Gaps = 7/227 (3%) Query: 88 LLPFAFEETLCRDVGTGLVPPSVHLWSHPGGVALGLRDSKLPRAAEAMAELEGKGYRTAV 147 L PF + E + + +H+W V LG+ D +LP A E+ GY Sbjct: 31 LSPFVWTEVFLKTINQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVT 90 Query: 148 RHSGGAAVPLDAGVVNVSLLLRKSPGK-LDFHDDFRLLASLITEAVAVSHPQAAAQIRAG 206 R+ GG AV D G++N SL++ + + + + ++ +I E S +I Sbjct: 91 RNIGGLAVVADDGILNFSLVIPDHFSESISISNAYLIMVDVIRE----SFSDYYQRIEYH 146 Query: 207 EVAGSYCPGDFDLAIGGRKFCGIAQRRQSSAYFVHAFVIVSGSGLERGQLIRGFYDTAAD 266 E+ SYCPG+FDL+I GRKF GIAQRR V ++ V G RGQLI+ FY+ Sbjct: 147 EIKNSYCPGNFDLSIAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQ 206 Query: 267 GD-DALAYPRVIPQTLAALGE-LGGPASAAVFAEGFRLALTRRGVHL 311 G+ + YP++ P+ +A L E L P + A E RL L + G L Sbjct: 207 GEVTKVNYPQIDPECMATLSELLETPFTVAEVLERLRLTLRQLGFSL 253 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,266,155 Number of extensions: 904385 Number of successful extensions: 1666 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1657 Number of HSP's successfully gapped: 6 Length of query: 345 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 238 Effective length of database: 26,037,693 Effective search space: 6196970934 Effective search space used: 6196970934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)