BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0023.p (270 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytbE [R] COG0656 Aldo/keto reductases, related to diketogulon... 413 e-115 BS_yvgN [R] COG0656 Aldo/keto reductases, related to diketogulon... 379 e-105 lin0819 [R] COG0656 Aldo/keto reductases, related to diketogulon... 356 2e-98 BH2158 [R] COG0656 Aldo/keto reductases, related to diketogulona... 350 2e-96 BH3849 [R] COG0656 Aldo/keto reductases, related to diketogulona... 339 3e-93 >BS_ytbE [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 413 bits (1062), Expect = e-115, Method: Compositional matrix adjust. Identities = 16/270 (5%), Positives = 43/270 (15%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L Q E Q + R Q + E Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 N LA+ + + G + L + L + + Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 + V + + + Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 +++ + + + +++ + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 R + ++ + D Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDI 280 >BS_yvgN [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 379 bits (973), Expect = e-105, Method: Compositional matrix adjust. Identities = 19/270 (7%), Positives = 49/270 (18%), Gaps = 4/270 (1%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L Q E Q + R Q + E Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET--- 67 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 + S+ + + + + + + L Sbjct: 68 -YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 + + + D + ++ + + + E + Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 + L L + V + + +V L Q+ V + + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 + G + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAA 276 >lin0819 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 356 bits (914), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 16/270 (5%), Positives = 44/270 (16%), Gaps = 4/270 (1%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 + V+ G F + A ++ A + Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT--- 65 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 + + + + + + D + Sbjct: 66 -ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 + F ++ + L Y ++R Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 L D P + I + + + + Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGK 244 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 + R ++ R + Sbjct: 245 YLNGAKPAGARNTLFSRFTRYSGEQTQKAV 274 >BH2158 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 350 bits (897), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 19/270 (7%), Positives = 49/270 (18%), Gaps = 4/270 (1%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L Q E Q + R Q + E Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET--- 67 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 + S+ + + + + + + L Sbjct: 68 -YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 + + + D + ++ + + + E + Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 + L L + V + + +V L Q+ V + + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 + G + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAA 276 >BH3849 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 339 bits (869), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 19/270 (7%), Positives = 49/270 (18%), Gaps = 4/270 (1%) Query: 1 LNSGVNMPWFGLGVFQVEEGAELIQAVKSAIAYGYRSVDTAAIYQNERGVGQAVAEALAE 60 L Q E Q + R Q + E Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET--- 67 Query: 61 NQLAREDLFITSKVWNADLGYEATLAAYDASLEKLGLDYLDLYLIHWPVQGKYKDAWRAL 120 + S+ + + + + + + L Sbjct: 68 -YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 121 ETLYTSGRVKAIGVSNFQIHHLEDVIRDAKITPMVNQVELHPYLSQRELRRFCKEQGIQI 180 + + + D + ++ + + + E + Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 181 EAWSPLMQGQLLDQPVLKEIAAAHGKSVAQVILRWDLQHGIVTIPKSTKEHRIIENASVF 240 + L L + V + + +V L Q+ V + + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 241 DFELSTGDMERIDGLNQDKRTGPDPDHFDF 270 + G + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAA 276 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,483,355 Number of extensions: 850959 Number of successful extensions: 15240 Number of sequences better than 1.0e-05: 6303 Number of HSP's gapped: 15021 Number of HSP's successfully gapped: 6304 Length of query: 270 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 165 Effective length of database: 26,268,361 Effective search space: 4334279565 Effective search space used: 4334279565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)