BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0016.p (434 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pbuX [F] COG2233 Xanthine/uracil permeases 437 e-122 BS_yunK [F] COG2233 Xanthine/uracil permeases 424 e-118 lin1997 [F] COG2233 Xanthine/uracil permeases 419 e-117 L160092 [F] COG2233 Xanthine/uracil permeases 370 e-102 SPy1137 [F] COG2233 Xanthine/uracil permeases 369 e-102 >BS_pbuX [F] COG2233 Xanthine/uracil permeases Length = 438 Score = 437 bits (1123), Expect = e-122, Method: Compositional matrix adjust. Identities = 220/408 (53%), Positives = 290/408 (71%) Query: 6 KILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIISSKHF 65 K L+LG+QHVLAMYAGA++VPL+VG A+ L+ Q+ YL++ D+F CG+ATLLQ+ S++ F Sbjct: 7 KTLSLGIQHVLAMYAGAIVVPLIVGKAMGLTVEQLTYLVSIDIFMCGVATLLQVWSNRFF 66 Query: 66 GSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFPXXXX 125 G GLPVVLGCTFTAVSP+IAI S ++T YG+II SG+ V+L + +GKL+ FFP Sbjct: 67 GIGLPVVLGCTFTAVSPMIAIGSEYGVSTVYGSIIASGILVILISFFFGKLVSFFPPVVT 126 Query: 126 XXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMRSVSV 185 ++L+PVAMNN+AGG+GSADFG N + RFT GF++SVS+ Sbjct: 127 GSVVTIIGITLMPVAMNNMAGGEGSADFGDLSNLALAFTVLSIIVLLYRFTKGFIKSVSI 186 Query: 186 LVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVSMVES 245 L+G++ GT I Y MG V F V A+ V + QPFYFG P F I TM IV IVS+VES Sbjct: 187 LIGILIGTFIAYFMGKVQFDNVSDAAVVQMIQPFYFGAPSFHAAPIITMSIVAIVSLVES 246 Query: 246 TGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTRVKTR 305 TGVYFA+G T++++ + + G R+EGLA++LGG+FNAFPYTAFSQNVGL+ LT +K Sbjct: 247 TGVYFALGDLTNRRLTEIDLSKGYRAEGLAVLLGGIFNAFPYTAFSQNVGLVQLTGIKKN 306 Query: 306 NVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLRKDGN 365 VI G I++ GL PK+AA TT++P+AVLGGAM+ MFG V A G+ +LS +D K N Sbjct: 307 AVIVVTGVILMAFGLFPKIAAFTTIIPSAVLGGAMVAMFGMVIAYGIKMLSRIDFAKQEN 366 Query: 366 LLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNI 413 LLI ACS+ +GLG V+P +F QLP +L NGIV+GS TA++LNI Sbjct: 367 LLIVACSVGLGLGVTVVPDIFKQLPSALTLLTTNGIVAGSFTAVVLNI 414 >BS_yunK [F] COG2233 Xanthine/uracil permeases Length = 211 Score = 424 bits (1091), Expect = e-118, Method: Compositional matrix adjust. Identities = 0/422 (0%), Positives = 0/422 (0%) Query: 6 KILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIISSKHF 65 + L + P + A+ LS + + Sbjct: 9 ILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELG 68 Query: 66 GSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFPXXXX 125 G+ + S I + + L A Sbjct: 69 VEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNT 128 Query: 126 XXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMRSVSV 185 + + N + + + R + Sbjct: 129 AFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCI 188 Query: 186 LVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVSMVES 245 Sbjct: 189 248 Query: 246 TGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTRVKTR 305 Sbjct: 249 308 Query: 306 NVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLRKDGN 365 Sbjct: 309 368 Query: 366 LLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLSRTQENAAPA 425 Sbjct: 369 428 Query: 426 AT 427 Sbjct: 429 430 >lin1997 [F] COG2233 Xanthine/uracil permeases Length = 435 Score = 419 bits (1078), Expect = e-117, Method: Compositional matrix adjust. Identities = 216/430 (50%), Positives = 289/430 (67%) Query: 1 MLSKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQII 60 ML K KI ALG QHVLAMYAGAVIVPL++GGAL +G +M YL++ D+F CG+ATLLQ+ Sbjct: 1 MLGKGKIAALGFQHVLAMYAGAVIVPLLIGGALGFNGEEMTYLVSIDIFMCGIATLLQLT 60 Query: 61 SSKHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFF 120 ++ FG GLPVVLGC A++PII I + YG+II+SGLFV+L AP + K+++FF Sbjct: 61 VNRFFGIGLPVVLGCAVQAIAPIILIGQDMGIGAIYGSIIVSGLFVLLIAPFFSKVVRFF 120 Query: 121 PXXXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFM 180 P L+LIPVA+NN+AGG G+ DFG+ N V RF GF Sbjct: 121 PPVVTGSVVTVIGLTLIPVAINNLAGGAGAKDFGSMYNLGLGFGTLLLIILVYRFGQGFS 180 Query: 181 RSVSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIV 240 ++++VL+GLV G++ + V ASW + +PFYFG P F AI TMI++ +V Sbjct: 181 KAIAVLIGLVGGSLFAALYKGISLGPVSEASWFHMPKPFYFGTPTFEWPAIITMILIALV 240 Query: 241 SMVESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLT 300 SMVESTGVYFA+ T++++ QK + G R+EGLAIMLGG+FN FPYTA+SQNVGL+ L+ Sbjct: 241 SMVESTGVYFALSDITERKLTQKDLTRGYRAEGLAIMLGGVFNTFPYTAYSQNVGLVQLS 300 Query: 301 RVKTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDL 360 +KTR VI+AA G ++VLGL+PK+ A+TT++P VLGGAM+ MFG V A G+ +L +V+ Sbjct: 301 GIKTRKVIYAAAGFLIVLGLIPKIGAVTTIIPTPVLGGAMVAMFGMVVAQGIKMLGKVNF 360 Query: 361 RKDGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLSRTQE 420 NLLI AC++ VGLG V+P +F+ P F R+ NGIV+GS+TAI LNI + Sbjct: 361 TSQENLLIIACAVGVGLGVTVVPDLFNAFPSFVRLFTSNGIVAGSVTAITLNIIFNMIPH 420 Query: 421 NAAPAATVPE 430 PE Sbjct: 421 RKDKKLADPE 430 >L160092 [F] COG2233 Xanthine/uracil permeases Length = 434 Score = 370 bits (951), Expect = e-102, Method: Compositional matrix adjust. Identities = 187/419 (44%), Positives = 278/419 (66%), Gaps = 4/419 (0%) Query: 3 SKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIISS 62 S K LGLQH+LAMY+G+++VP+++ GALN S TQ+ YLI+AD+F CGLAT LQ+ Sbjct: 12 SNSKSAVLGLQHLLAMYSGSILVPIMIAGALNYSATQLTYLISADIFMCGLATFLQLQLR 71 Query: 63 KHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFPX 122 KHFG GLPVVLG F +V+P+I I +G+++++G+FV+L + ++ K+ K FP Sbjct: 72 KHFGIGLPVVLGVAFQSVAPLIIIGQRHGSGAMFGSLMVAGVFVILVSGIFSKIRKLFPP 131 Query: 123 XXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMRS 182 LSLIPVA+ N+ + D ++ +N FTTGF+RS Sbjct: 132 IVTGSVITTIGLSLIPVAIGNMGN---NVDKPTIQSLILAVSTIVIILLINIFTTGFIRS 188 Query: 183 VSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVSM 242 +++L+GL+AGT++ +MG+V FS V A W + QPFYF P+F + MII+ IVS+ Sbjct: 189 IAILIGLIAGTILAASMGLVDFSVVSQAPWAHLPQPFYFSAPKFYLADSLMMIIIAIVSL 248 Query: 243 VESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTRV 302 VESTGVY A+ T + +++K++ NG R+EG A+ LGG+FN FPYT FSQNVGL+ L+ + Sbjct: 249 VESTGVYLALADITGENLDEKRLRNGYRAEGFAVFLGGVFNTFPYTGFSQNVGLVQLSGI 308 Query: 303 KTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLRK 362 KTR I+ G ++VLGL+PK AA+ ++P VLGGAM++MFG VA G+ +L++V+ Sbjct: 309 KTRKPIYFTAGFLIVLGLIPKFAAVAQLIPTPVLGGAMLIMFGMVATQGIRMLAKVEFEG 368 Query: 363 DGNLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLSRTQEN 421 + NLLIAA +IA+G+G ++F LP F + + NGIV +++AIILN+ + +++ Sbjct: 369 NQNLLIAAVAIAMGVGFNS-TNLFTALPSFIQPFVSNGIVMSTVSAIILNLVFNHGKKD 426 >SPy1137 [F] COG2233 Xanthine/uracil permeases Length = 427 Score = 369 bits (948), Expect = e-102, Method: Compositional matrix adjust. Identities = 194/420 (46%), Positives = 279/420 (66%), Gaps = 5/420 (1%) Query: 3 SKQKILALGLQHVLAMYAGAVIVPLVVGGALNLSGTQMAYLIAADLFTCGLATLLQIISS 62 S + LGLQHVL+MYAG+++VP+++ GAL S ++ YLI+ D+F CG+AT LQ+ + Sbjct: 10 SHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSARELTYLISTDIFMCGVATFLQLKLT 69 Query: 63 KHFGSGLPVVLGCTFTAVSPIIAIASGSNLATAYGAIIISGLFVVLAAPVYGKLLKFFPX 122 KH G GLPVVLGC F +V+P+ I + +GA+I SG++V+L A ++ K+ +FFP Sbjct: 70 KHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAMFGALIASGIYVILVAGIFSKIARFFPP 129 Query: 123 XXXXXXXXXXXLSLIPVAMNNVAGGQGSADFGAPRNXXXXXXXXXXXXAVNRFTTGFMRS 182 LSL+ VAM N+ G + A ++ V +FT GF++S Sbjct: 130 IVTGSVITVIGLSLVGVAMGNM--GDNVKEPTA-QSMMLSLLTIVIILLVQKFTKGFVKS 186 Query: 183 VSVLVGLVAGTVIGYAMGIVHFSTVGSASWVSIAQPFYFGWPEFSITAIFTMIIVNIVSM 242 +S+L+GLVAGT++ MG+V + V ASW+ + PFYFG P F IT+I M I+ VSM Sbjct: 187 ISILIGLVAGTLVSAMMGLVDTTPVVEASWIHVPTPFYFGMPTFEITSIVMMCIIATVSM 246 Query: 243 VESTGVYFAVGKATDQQVEQKQVVNGLRSEGLAIMLGGLFNAFPYTAFSQNVGLLSLTRV 302 VESTGVY A+ T+ Q+++K++ NG RSEG+A+ LGGLFN FPYT FSQNVGL+ ++ + Sbjct: 247 VESTGVYLALSDLTNDQLDEKRLRNGYRSEGIAVFLGGLFNTFPYTGFSQNVGLVQISGI 306 Query: 303 KTRNVIFAAGGIMVVLGLLPKLAALTTVVPNAVLGGAMIVMFGSVAASGMSILSEVDLRK 362 KTR I+ A GI+VV+GLLPK A+ ++P+ VLGGAM+V+FG VA GM +L+ VD +K Sbjct: 307 KTRRPIYYAAGILVVIGLLPKFRAMAQMIPSPVLGGAMLVLFGMVALQGMQMLNRVDFQK 366 Query: 363 DG-NLLIAACSIAVGLGSAVLPSMFDQLPEFARMLLQNGIVSGSLTAIILNIFLSRTQEN 421 + N +IAA SI+ GLG ++F LPE A+M L NGIV +LT+++LN+ L+ + Sbjct: 367 NEYNFIIAAVSISAGLGFNG-TNLFASLPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQ 425 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.138 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,872,258 Number of extensions: 1052140 Number of successful extensions: 4804 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 4617 Number of HSP's successfully gapped: 66 Length of query: 434 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 325 Effective length of database: 25,807,025 Effective search space: 8387283125 Effective search space used: 8387283125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)