BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0013.p (449 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 96 1e-19 SA0132 [GEPR] COG0477 Permeases of the major facilitator superfa... 75 2e-13 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 72 2e-12 PM1784 [GEPR] COG0477 Permeases of the major facilitator superfa... 71 4e-12 SPy0570 [GEPR] COG0477 Permeases of the major facilitator superf... 69 2e-11 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 95.5 bits (236), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 78/320 (24%), Positives = 140/320 (43%), Gaps = 11/320 (3%) Query: 15 FIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRLSDXXXXXXXXX 74 F+ ++N T+ NV+LP+I++D I+A G W+ + + + + + + L + Sbjct: 22 FVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIERFTTRQLYL 81 Query: 75 XXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVPVARRGATIAMI 134 + IG FA + +L+ R++Q+ P+ RRG + +I Sbjct: 82 FAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLI 141 Query: 135 SVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKEQPQAAV--FDLY 192 + A +GP + G I Q + W LF + K + D+ Sbjct: 142 GLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKLDIL 201 Query: 193 GAALTVINAGSLLVAVTSQ-SYIWLAVSVASF-----AVHAIHLR---RARDSFINPKLL 243 G ++ + GSLL+ ++ + WL VA F V I +R + +N K+ Sbjct: 202 GVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVLGIFIRYQSSNKAPLLNFKVF 261 Query: 244 QMPGYLKLTAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFLTRFVG 303 + P + TAI F +++ G + L+P+ L + S L GL + PGA+++A L+ G Sbjct: 262 KYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSPVTG 321 Query: 304 RWIDRYGNLRFLLIGHGILA 323 DR+G L+G I+A Sbjct: 322 VMFDRFGAKYLSLVGLIIMA 341 >SA0132 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 450 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 87/403 (21%), Positives = 167/403 (41%), Gaps = 10/403 (2%) Query: 1 VNDKVVMPLWTFCLFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIY 60 + V + L+ F +V+ N+ F + I DL+IS SW + + + V+Y Sbjct: 8 IKKAVPILLFLFVFSLVIDNS--FKLISVAIADDLNISVTTVSWQATLAGLVIGIGAVVY 65 Query: 61 SRLSDXXXX-XXXXXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXS 119 + LSD + + +F H F +LL+ RI+Q+ + Sbjct: 66 ASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLLLVGRIIQTAGLAAAETLYVIYVA 125 Query: 120 RYVPVARRGATIAMISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXX 179 +Y+ + + + + +++ +G + GG IS Y W +F + Sbjct: 126 KYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFISTYLHWTNMFLIALIVVFTLPFLFKLL 185 Query: 180 XKEQ-PQAAVFDLYGAALTVINAGSLLVAVTSQSYIWLAVSVASFAVHAIHLRRARDSFI 238 KE A D G L A ++++ +T+ +++++ ++ + V A++++ A+ + Sbjct: 186 PKENNTNKAHLDFVGLILVATIATTVMLFITNFNWLYMIGALIAIIVFALYIKNAQRPLV 245 Query: 239 NPKLLQMPGYLKLTAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFL 298 N Q Y I F + + LG +F P + ++H L + PG I++ + Sbjct: 246 NKSFFQNKRYASFLFIVFVMYAIQLGYIFTFPFIMEQIYHLQLDTTSLLLVPGYIVAVIV 305 Query: 299 TRFVGRWIDRYGNLR---FLLIGHGILATVLAVFALGLQVSPLVLLFGYLCFSPAFSATM 355 G+ I Y N + I L+ +L FA+G +S V+ + F+ +F+ Sbjct: 306 GALSGK-IGEYLNSKQAIITAIILIALSLILPAFAVGNHISIFVI--SMIFFAGSFALMY 362 Query: 356 ASLNNEASGILPRTWIGSGMGLLQLIQFFGGSVSVAVCGLLLH 398 A L NEA + G +G LI SV +A+ L+ Sbjct: 363 APLLNEAIKTIDLNMTGVAIGFYNLIINVAVSVGIAIAAALID 405 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 83/405 (20%), Positives = 157/405 (38%), Gaps = 17/405 (4%) Query: 6 VMPLWTFCL---FIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSR 62 VMP+ L FI + + T N++L ++++L+I+A W+ + Y + + + Sbjct: 15 VMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGL 74 Query: 63 LSDXXXXXXXXXXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYV 122 L + I A SF+ LL RI+Q+ Sbjct: 75 LLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIF 134 Query: 123 PVARRGATIAMISVGSAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKE 182 P +RGA + I + A +GP GL+ ++ W+ +F ++ + Sbjct: 135 PPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQN 194 Query: 183 --QPQAAVFDLYGAALTVINAGSLLVAVTSQSY-----------IWLAVSVASFAVHAIH 229 + D+ L+ I G ++ ++ + L V V + +I Sbjct: 195 VSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIR 254 Query: 230 LRRARDSFINPKLLQMPGYLKLTAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIA 289 + +N + + P ++ + F +++ L ++ L+P+ L +A GL + Sbjct: 255 QLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLL 314 Query: 290 PGAILSAFLTRFVGRWIDRYGNLRFLLIGHGILATVLAVFALGLQVSPLVLLFG-YLCFS 348 PG IL+ F++ GR D+YG ++ G I+ VL F+ S VL+ + C Sbjct: 315 PGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLM 374 Query: 349 PAFSATMASLNNEASGILPRTWIGSGMGLLQLIQFFGGSVSVAVC 393 S M LPR + G ++ +Q G++ AV Sbjct: 375 IGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVA 419 >PM1784 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 461 Score = 70.9 bits (172), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 101/417 (24%), Positives = 165/417 (39%), Gaps = 30/417 (7%) Query: 14 LFIVVMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRLSDXXXXXXXX 73 LF+ ++ T+ N +LP I +DL+ S I SY++ AL + L+D Sbjct: 18 LFMQTLDATILNTALPAISRDLNESPLEMQLAIISYALSVALFIPLSGWLADKYGTLKIF 77 Query: 74 XXXXXXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVPVARRGATIAM 133 SV +++ + L++ R+LQ R VP + + Sbjct: 78 RIAVSIFVIGSVACAISNTLDTLVLARVLQGFGGALMMPVARLAIIRTVPKQHLLSVWNL 137 Query: 134 ISVGSAMAFGLGPIVGGLISQYFGWNGLF------GVTCXXXXXXXXXXXXXXKEQPQAA 187 +++ + LGPI+GG + Y W+ +F G+ + Sbjct: 138 MAMSGLLGPILGPILGGWLVTYATWHWIFLINIPIGIIGVLIAGNYMQNVTAEVNKLDWK 197 Query: 188 VFDLYGAALTVINAGSLLVAVTSQSYIWLAVSVASF-----AVHAIHLRRARDSFINPKL 242 F L+ +L + G L+A S S+ +A SV + H +++ S + L Sbjct: 198 GFMLFAGSLVGLTLGLDLIAERS-SHKGIAFSVLMLGLLLLCAYYFHAKKSPISLLPLSL 256 Query: 243 LQMPGYLKLTAIGFSILVLNLGNLFLMPLALSNLFHQSALMIGLYIAPGAILSAFLTRFV 302 ++ + I + G FL+PL L +FH SA + G +AP AI S + + Sbjct: 257 FKIRTFTISIVANLFIRLCGSGIPFLLPLMLQVVFHYSAELSGWLLAPIAISSVLMKPLI 316 Query: 303 GRWIDRYGNLRFLLIGHGILATVLAVFALGLQVSPLVLL-----FGYLCFSPAFSATMAS 357 + R G LL+ L +AV AL +P+ LL F C S FSAT Sbjct: 317 SPILHRLGYKTTLLLTAICLTLAIAVMALVTVDTPIWLLVLALAFYGACMSVIFSAT--- 373 Query: 358 LNNEASGILPRTWIGSGMGLLQLIQFFGGSVSVAVCGLLLHAQRTLAPSGAYRNVYG 414 N A L +G +L + Q G + +AVC ++L G YR+ G Sbjct: 374 -NTLAVSELSEQNASAGSTILSVAQQMGIGIGIAVCSVIL---------GIYRDHIG 420 >SPy0570 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 455 Score = 68.9 bits (167), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 84/398 (21%), Positives = 162/398 (40%), Gaps = 22/398 (5%) Query: 18 VMNTTMFNVSLPTIIQDLHISADLGSWVISSYSIGYALSTVIYSRLSDXXXXXXXXXXXX 77 +++ T NV+ PT++ + + W+ + Y + A+ + L Sbjct: 27 ILSETSMNVTFPTLMSVYQLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMAT 86 Query: 78 XXXXXASVIGLFAHSFNVLLITRILQSXXXXXXXXXXXXXXSRYVPVARRGATIAMISVG 137 +++ + SF ++L+ RI Q VP+ + G + + Sbjct: 87 GLFTFGTILAVLTQSFAIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLI 146 Query: 138 SAMAFGLGPIVGGLISQYFGWNGLFGVTCXXXXXXXXXXXXXXKEQP--QAAVFDLYGAA 195 ++A GP GG + +F W +F ++ P + FD Sbjct: 147 ISLAPAFGPTYGGFMISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFI 206 Query: 196 LTVINAGSLLVAVTSQ-----SYIWLAVSVASFAVHAIHLRRARDSFINPKLLQMPGYLK 250 I+ S L+A+TS + +L + + SF + A+ F++ ++L++P L Sbjct: 207 ALSISLTSALLAITSLENGSVNLYYLGLFILSFILFLYKNLTAKQPFLDIRILKIPS-LT 265 Query: 251 LTAIGFSIL-VLNLGNLFLMP--LALSNLFHQSALMIGLYIAPGAILSAFLTRFVGRWID 307 I F + ++NLG FL P + + + + S G+ + PG +L A L G+ D Sbjct: 266 FGLIPFFVFQLINLGINFLTPNFIVMEKIANSSQ--AGMVLLPGTLLGALLAPAFGKLYD 323 Query: 308 RYGNLRFLLIGHGILATVLAVFALG---LQVSPLVLLFGYLCFSPAFSATMASLNNEASG 364 + G L +G+ + + L + L + P LL Y+ F+ F M N+ A+ Sbjct: 324 QKGARLSLYLGNALFSLSLIIMTLQTRHFMLLPFTLL--YILFT--FGRNMGFNNSLATA 379 Query: 365 I--LPRTWIGSGMGLLQLIQFFGGSVSVAVCGLLLHAQ 400 I LP + Q++Q F G++ A+ L+ ++Q Sbjct: 380 IRELPAEKNADATAIFQMMQQFAGALGTAMASLIANSQ 417 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,641,751 Number of extensions: 958494 Number of successful extensions: 9838 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 9812 Number of HSP's successfully gapped: 51 Length of query: 449 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 339 Effective length of database: 25,691,691 Effective search space: 8709483249 Effective search space used: 8709483249 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)