BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0010.p (405 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZyjfC [E] COG0754 Glutathionylspermidine synthase 104 2e-22 ECs5162 [E] COG0754 Glutathionylspermidine synthase 104 2e-22 BS_yjfC [O] COG5504 Predicted Zn-dependent protease 103 5e-22 ZyjfC [E] COG0754 Glutathionylspermidine synthase 103 5e-22 yjfC [E] COG0754 Glutathionylspermidine synthase 103 5e-22 >ZyjfC [E] COG0754 Glutathionylspermidine synthase Length = 387 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 112/394 (28%), Positives = 181/394 (45%), Gaps = 59/394 (14%) Query: 29 GFTWADLEDEEYWLDAVA--VMRGETYKELEEASVKLWSILDKAVRYVHRRHDLYDLLGI 86 GF + +++E YW ++ A + +++E+ + +L + + V + ++ L I Sbjct: 20 GFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAI 79 Query: 87 PPVLWQMLDDT-PLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTG-YVEASVATPWI 144 PP+ W ++ ++ +P L Y R DFA G+ +KLLE NADTPT Y A W+ Sbjct: 80 PPLYWDVIAESWRARDPSL---YGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWL 136 Query: 145 --CGQAGISSRN----NGMKERLAAAWS---IEQPDTAACVAYGTHQEDSGTI---EALV 192 ++GI R+ N ++ERL + +S +P C ED T+ + Sbjct: 137 EDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQ--DTDEDRSTVLYLQDCA 194 Query: 193 AHSGLDIRCVDCLDLWIDEGTV-RDAENRIIKRMFALYPKEWMAVDDGGEALAYAIESGQ 251 +G + R + DL + G V D ++ +I+R F LYP EWM DD G L E Sbjct: 195 QQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWV 254 Query: 252 LQLFNGPHSILLQSKGLIAAVWGMY--ELGLLFS--EDERQAIA---SYIL-PTYNKPXX 303 L+ +L +KGL+ +W + LL S + E+ IA SY+ P Y+ Sbjct: 255 EPLWKS----ILSNKGLMPLLWRFFPGHPNLLASWFDGEKPQIAAGESYVRKPIYS---- 306 Query: 304 XXXXXXXXXXXREGGSVRIFD-DSGALELADEDGFDSSVLFPTVYQKRAELARIQTPEGE 362 REGG+V IFD + ++ AD D D P +YQ L R G+ Sbjct: 307 -----------REGGNVTIFDGKNNVVDHADGDYADE----PMIYQAFQPLPRF----GD 347 Query: 363 LHLLTGMFVINGVPCGLLGRAGGP-ITGNASHFI 395 + L G ++++ CG+ R IT + S F+ Sbjct: 348 SYTLIGSWIVDDEACGMGIREDNTLITKDTSRFV 381 >ECs5162 [E] COG0754 Glutathionylspermidine synthase Length = 211 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 0/394 (0%), Positives = 0/394 (0%), Gaps = 59/394 (14%) Query: 29 GFTWADLEDEEYWLDAVA--VMRGETYKELEEASVKLWSILDKAVRYVHRRHDLYDLLGI 86 T + L AV + A + + A+ + + I Sbjct: 20 PTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTI 79 Query: 87 PPVLWQMLDDT-PLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTG-YVEASVATPWI 144 + + + L + T A Sbjct: 80 GLYNSKAENIIKTCRILLE---QHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIA 136 Query: 145 --CGQAGISSRN----NGMKERLAAAWS---IEQPDTAACVAYGTHQEDSGTI---EALV 192 + +R E++ + T + Sbjct: 137 VDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHW--LILHGRYTCIARKPRC 194 Query: 193 AHSGLDIRCVDCLDLWIDEGTV-RDAENRIIKRMFALYPKEWMAVDDGGEALAYAIESGQ 251 Sbjct: 195 254 Query: 252 LQLFNGPHSILLQSKGLIAAVWGMY--ELGLLFS--EDERQAIA---SYIL-PTYNKPXX 303 Sbjct: 255 -------- 306 Query: 304 XXXXXXXXXXXREGGSVRIFD-DSGALELADEDGFDSSVLFPTVYQKRAELARIQTPEGE 362 Sbjct: 307 ------------------- 347 Query: 363 LHLLTGMFVINGVPCGLLGRAGGP-ITGNASHFI 395 Sbjct: 348 381 >BS_yjfC [O] COG5504 Predicted Zn-dependent protease Length = 387 Score = 103 bits (257), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 110/394 (27%), Positives = 179/394 (45%), Gaps = 59/394 (14%) Query: 29 GFTWADLEDEEYWLDAVA--VMRGETYKELEEASVKLWSILDKAVRYVHRRHDLYDLLGI 86 GF + +++E YW ++ A + +++E+ + +L + + V + ++ L I Sbjct: 20 GFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAI 79 Query: 87 PPVLWQMLDDT-PLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTG-YVEASVATPWI 144 PP+ W ++ ++ +P L Y R DFA G+ +KLLE NADTPT Y A W+ Sbjct: 80 PPLYWDVIAESWRARDPSL---YGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWL 136 Query: 145 --CGQAGISSRN----NGMKERLAAAWS---IEQPDTAACVAYGTHQEDSGTI---EALV 192 ++GI R+ N ++ERL + +S +P C ED T+ + Sbjct: 137 EDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQ--DTDEDRSTVLYLQDCA 194 Query: 193 AHSGLDIRCVDCLDLWIDEGTV-RDAENRIIKRMFALYPKEWMAVDDGGEALAYAIESGQ 251 +G + R + DL + G V D ++ +I+R F LYP EWM DD G L E Sbjct: 195 QQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWV 254 Query: 252 LQLFNGPHSILLQSKGLIAAVWGMYE-----LGLLFSEDERQAIA--SYIL-PTYNKPXX 303 L+ +L +KGL+ +W + L F ++ Q A SY+ P Y+ Sbjct: 255 EPLWKS----ILSNKGLMPLLWRFFPGHPNLLASWFDGEKPQIAAGESYVRKPIYS---- 306 Query: 304 XXXXXXXXXXXREGGSVRIFD-DSGALELADEDGFDSSVLFPTVYQKRAELARIQTPEGE 362 REGG+V IFD + ++ AD D D P +YQ L R G+ Sbjct: 307 -----------REGGNVTIFDGKNNVVDHADGDYADE----PMIYQAFQPLPRF----GD 347 Query: 363 LHLLTGMFVINGVPCGLLGRAGGP-ITGNASHFI 395 + L G ++++ CG+ R IT + S F+ Sbjct: 348 SYTLIGSWIVDDEACGMGIREDNTLITKDTSRFV 381 Score = 103 bits (257), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 110/394 (27%), Positives = 179/394 (45%), Gaps = 59/394 (14%) Query: 29 GFTWADLEDEEYWLDAVA--VMRGETYKELEEASVKLWSILDKAVRYVHRRHDLYDLLGI 86 GF + +++E YW ++ A + +++E+ + +L + + V + ++ L I Sbjct: 20 GFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAI 79 Query: 87 PPVLWQMLDDT-PLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTG-YVEASVATPWI 144 PP+ W ++ ++ +P L Y R DFA G+ +KLLE NADTPT Y A W+ Sbjct: 80 PPLYWDVIAESWRARDPSL---YGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWL 136 Query: 145 --CGQAGISSRN----NGMKERLAAAWS---IEQPDTAACVAYGTHQEDSGTI---EALV 192 ++GI R+ N ++ERL + +S +P C ED T+ + Sbjct: 137 EDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQ--DTDEDRSTVLYLQDCA 194 Query: 193 AHSGLDIRCVDCLDLWIDEGTV-RDAENRIIKRMFALYPKEWMAVDDGGEALAYAIESGQ 251 +G + R + DL + G V D ++ +I+R F LYP EWM DD G L E Sbjct: 195 QQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWV 254 Query: 252 LQLFNGPHSILLQSKGLIAAVWGMYE-----LGLLFSEDERQAIA--SYIL-PTYNKPXX 303 L+ +L +KGL+ +W + L F ++ Q A SY+ P Y+ Sbjct: 255 EPLWKS----ILSNKGLMPLLWRFFPGHPNLLASWFDGEKPQIAAGESYVRKPIYS---- 306 Query: 304 XXXXXXXXXXXREGGSVRIFD-DSGALELADEDGFDSSVLFPTVYQKRAELARIQTPEGE 362 REGG+V IFD + ++ AD D D P +YQ L R G+ Sbjct: 307 -----------REGGNVTIFDGKNNVVDHADGDYADE----PMIYQAFQPLPRF----GD 347 Query: 363 LHLLTGMFVINGVPCGLLGRAGGP-ITGNASHFI 395 + L G ++++ CG+ R IT + S F+ Sbjct: 348 SYTLIGSWIVDDEACGMGIREDNTLITKDTSRFV 381 >yjfC [E] COG0754 Glutathionylspermidine synthase Length = 387 Score = 103 bits (257), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 110/394 (27%), Positives = 179/394 (45%), Gaps = 59/394 (14%) Query: 29 GFTWADLEDEEYWLDAVA--VMRGETYKELEEASVKLWSILDKAVRYVHRRHDLYDLLGI 86 GF + +++E YW ++ A + +++E+ + +L + + V + ++ L I Sbjct: 20 GFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAI 79 Query: 87 PPVLWQMLDDT-PLPEPGLISRYARFDFAIAGDGTIKLLELNADTPTG-YVEASVATPWI 144 PP+ W ++ ++ +P L Y R DFA G+ +KLLE NADTPT Y A W+ Sbjct: 80 PPLYWDVIAESWRARDPSL---YGRMDFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWL 136 Query: 145 --CGQAGISSRN----NGMKERLAAAWS---IEQPDTAACVAYGTHQEDSGTI---EALV 192 ++GI R+ N ++ERL + +S +P C ED T+ + Sbjct: 137 EDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQ--DTDEDRSTVLYLQDCA 194 Query: 193 AHSGLDIRCVDCLDLWIDEGTV-RDAENRIIKRMFALYPKEWMAVDDGGEALAYAIESGQ 251 +G + R + DL + G V D ++ +I+R F LYP EWM DD G L E Sbjct: 195 QQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWV 254 Query: 252 LQLFNGPHSILLQSKGLIAAVWGMYE-----LGLLFSEDERQAIA--SYIL-PTYNKPXX 303 L+ +L +KGL+ +W + L F ++ Q A SY+ P Y+ Sbjct: 255 EPLWKS----ILSNKGLMPLLWRFFPGHPNLLASWFDGEKPQIAAGESYVRKPIYS---- 306 Query: 304 XXXXXXXXXXXREGGSVRIFD-DSGALELADEDGFDSSVLFPTVYQKRAELARIQTPEGE 362 REGG+V IFD + ++ AD D D P +YQ L R G+ Sbjct: 307 -----------REGGNVTIFDGKNNVVDHADGDYADE----PMIYQAFQPLPRF----GD 347 Query: 363 LHLLTGMFVINGVPCGLLGRAGGP-ITGNASHFI 395 + L G ++++ CG+ R IT + S F+ Sbjct: 348 SYTLIGSWIVDDEACGMGIREDNTLITKDTSRFV 381 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,490,111 Number of extensions: 1438959 Number of successful extensions: 2173 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2145 Number of HSP's successfully gapped: 12 Length of query: 405 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 296 Effective length of database: 25,807,025 Effective search space: 7638879400 Effective search space used: 7638879400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)