BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0007.p (337 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3340 [P] COG1226 Kef-type K+ transport systems, predicted NAD-... 130 3e-30 BH1971 [P] COG1226 Kef-type K+ transport systems, predicted NAD-... 94 4e-19 TVN0988 [P] COG1226 Kef-type K+ transport systems, predicted NAD... 87 4e-17 MTH1520 [P] COG1226 Kef-type K+ transport systems, predicted NAD... 86 9e-17 aq_1863 [P] COG1226 Kef-type K+ transport systems, predicted NAD... 81 2e-15 >BH3340 [P] COG1226 Kef-type K+ transport systems, predicted NAD-binding component Length = 338 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 76/259 (29%), Positives = 128/259 (49%), Gaps = 3/259 (1%) Query: 37 LEPGTFSNWFNAFYWVMTTMSTVGYGDFFAGTVAGKLFTIFLYIFGIGLLSLVIGKIIDA 96 LEP F F+ +W + T+STVGYGDF T+ G+L I L + G+G ++ + + Sbjct: 38 LEPEAFPTLFDGIWWAIVTISTVGYGDFVPYTLIGRLLGIVLIMSGVGFMTFYVTTLAAT 97 Query: 97 MGELQRRRGAGTLSFHGKDHVVLINWNRKTQAAVDEILCYTPDCRIVIIDESGQHPLQQM 156 + + G L KDH ++I WN +++ A+D +L + IV+IDE+ + Sbjct: 98 TIKSRNAYLEGKLHMTDKDHHIIIGWNERSRHAIDRLLEANENTTIVLIDETLEELPSTY 157 Query: 157 EHVHFISGDPSSDDILLKAGIEGAKAAIVFGDTRIDEASLTDGKTLLIASSIERIAPQVH 216 VHF+ G+ + D IL +A + A + ++ + E S D +++L +++ I P V+ Sbjct: 158 RRVHFVRGNSTEDAILQQANVREAASVLITAKHQGSEFS-ADSRSILTTLAVKSINPHVY 216 Query: 217 TTVEIMQEKNIQNFRHVHVNEFVLSHDAISRLAVRSALQEGNSEVITQLLSREHGDDI-- 274 T VEI+ E I N +E V S + L S+++ +LL+ + Sbjct: 217 TVVEILTEGQIVNATRAGADEVVESTALTGAVLTNGLLHHNMSKILDRLLTEGEKKQLFF 276 Query: 275 YEIPRDAAWHTYGDAFQAL 293 Y IP T+ D FQ+L Sbjct: 277 YPIPEGMIGKTFDDYFQSL 295 >BH1971 [P] COG1226 Kef-type K+ transport systems, predicted NAD-binding component Length = 152 Score = 93.6 bits (231), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 53/152 (34%), Positives = 89/152 (58%), Gaps = 5/152 (3%) Query: 109 LSFHGKDHVVLINWNRKTQAAVDEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSS 168 + + GK+HVV+I W+ K + ++ EIL IV+ID+ + P+ ++V+++ GDPS Sbjct: 1 MVYQGKNHVVMITWSEKAKLSIMEILNEKKTTDIVVIDKLEEAPIDD-DNVYYVKGDPSK 59 Query: 169 DDILLKAGIEGAKAAIVFGDTRIDEASLTDGKTLLIASSIERIAPQVHT----TVEIMQE 224 +IL KA + AK+ +F + + DGK LLIAS+I+++A +T EI+ E Sbjct: 60 VEILDKANVLEAKSVCLFASDNPVDVTAEDGKVLLIASTIKQMAKMKNTYPYIVCEILDE 119 Query: 225 KNIQNFRHVHVNEFVLSHDAISRLAVRSALQE 256 +I N V++++LSH S+L +AL E Sbjct: 120 DHINNGHADWVDDYILSHKPFSKLMAATALSE 151 >TVN0988 [P] COG1226 Kef-type K+ transport systems, predicted NAD-binding component Length = 348 Score = 87.0 bits (214), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 63/229 (27%), Positives = 112/229 (48%), Gaps = 20/229 (8%) Query: 11 LMRLKKKSIGLIILAFIV---ISATIAFLLEPGT----FSNWFNAFYWVMTTMSTVGYGD 63 L +++ + + +AFI+ I + + FL + N+F A ++ M T++TVGYGD Sbjct: 11 LQKIESSPLTKVFMAFIIVVLIGSYLEFLTQRNVKYSEIKNYFTAIWFTMETVTTVGYGD 70 Query: 64 FFAGTVAGKLFTIFLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLINWN 123 + G++ + + + GIGLL + I + +++ R G L K+H ++ NWN Sbjct: 71 VVPVSNLGRVVAMLIMVSGIGLLGTLTATISAYLFQIRIER-RGKLEKRLKNHTIICNWN 129 Query: 124 RKTQAAV----DEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEG 179 T+ + DE IVI+ E+ + ++ ++V F+ GD +S+D L A IE Sbjct: 130 AYTRNIIEGNRDE-----EAAPIVILSENTDNS-EKYQNVFFVKGDCTSEDDLKNAAIED 183 Query: 180 AKAAIVFGDTRID--EASLTDGKTLLIASSIERIAPQVHTTVEIMQEKN 226 A ++ D + L D KTLL ++ ++ V E+ EKN Sbjct: 184 AARVVIMSDIENNAIPEDLLDAKTLLSIFTVRKLNNAVEIIAEVRDEKN 232 >MTH1520 [P] COG1226 Kef-type K+ transport systems, predicted NAD-binding component Length = 268 Score = 85.9 bits (211), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 16/269 (5%), Positives = 49/269 (18%), Gaps = 14/269 (5%) Query: 10 KLMRLKKKSIGLIILAFIVISATIAFLLEPGTFSNWFNAFYWVMTTMSTVGYGDFFAGTV 69 I + ++ A F ++ + + + + + Sbjct: 14 LTAETGDLKIKTYKVV-LIARAASIFGVKRI--VIYHDDADGEARFIRDILTYMDTPQYL 70 Query: 70 AGKLFTIFLYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHGKDHVVLINWNRKTQAA 129 K+F I + +G+L + + G K ++ K Sbjct: 71 RRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDIGADKLALC 130 Query: 130 VDEILCYTPDCRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGAKAAIVFGDT 189 +++ + G+ L + + E K Sbjct: 131 REKL--TVNRIMSFRVVRLGKEILIE-PDEPEDRYWGYEVLDTRRNLAESLKTVGADVV- 186 Query: 190 RIDEASLTDGKTLLIASSIERIAPQVHTTVEIMQEKNIQNFRHVHVNEFVLSHDAISRLA 249 + + Sbjct: 187 -------VATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQ 239 Query: 250 VRSALQEGNSEVITQLLSREHGDDIYEIP 278 ++ + + T + + Sbjct: 240 CTETVRTEEAVLATLSVFNMLTQIDEKDE 268 >aq_1863 [P] COG1226 Kef-type K+ transport systems, predicted NAD-binding component Length = 476 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 15/231 (6%), Positives = 32/231 (13%), Gaps = 16/231 (6%) Query: 22 IILAFIVISATIAFLLEPG----TFSNWFNAFYWVMTTMSTVGYGDFFAGTVAGKLFTIF 77 + + + L + AG Sbjct: 142 VGIVSAITPFNFPLNLSMHKVAPALAAGNAVILKPSERTPLTPLMLGEILLEAGVPPKAL 201 Query: 78 LYIFGIGLLSLVIGKIIDAMGELQRRRGAGTLSFHG-KDHVVLINWNRKTQAAVDEILCY 136 I G G + + D L+ A + Sbjct: 202 SVIPGYGDVGKAMTTHPDVRVVSFTGSRKVGDIIARQVGIKKLVLELGSNSAIILHKDGN 261 Query: 137 TPD-----CRIVIIDESGQHPLQQMEHVHFISGDPSSDDILLKAGIEGAKAAIVFGDTRI 191 + + F+ D + I + Sbjct: 262 LEKAVEKTVQGGYAIA---GQVCISVQRVFVHEDLYDEFIKRLKERVSKLKVGDPMNEDT 318 Query: 192 D--EASLTDGKTLLIASSIERIAPQVHTTVEIMQEKNIQNFRHVHVNEFVL 240 D + I I + ++ Sbjct: 319 DLGPMIA-PSELERIQEWISEAVQKGAKIEAGGLRCAEDETIFSPTIVSLV 368 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,011,447 Number of extensions: 1046797 Number of successful extensions: 3574 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 3550 Number of HSP's successfully gapped: 25 Length of query: 337 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 230 Effective length of database: 26,037,693 Effective search space: 5988669390 Effective search space used: 5988669390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 116 (49.3 bits)