BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0084.p (319 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MT3575 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 84 3e-16 Rv3469c [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 84 3e-16 ZmhpE [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases 65 1e-10 mhpE [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases 65 1e-10 ZmhpE [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases 65 1e-10 >MT3575 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 334 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 62/242 (25%), Positives = 118/242 (48%), Gaps = 11/242 (4%) Query: 8 IVDCTIRDGGLVNNWDFSVEFVQNLYAGLNEAGVDYMEIGYKNSPKLLKGAEGAGPWRFL 67 ++D T+RDG N+ ++ + V+ + L+ AG+ Y+EIG+ + + A GP Sbjct: 10 VLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVT---IGAAAAQGPAAHT 66 Query: 68 NDDFLRKVIPQKGHTKLSALV--DIGRVDENDILPRSESMLDLIRVACYSKDVDKALQLV 125 ++++ R + +L A++ + R++ D+ + LD +R+ + + + + V Sbjct: 67 DEEYFRAARSVVRNARLGAVIVPALARIETVDL---AGDYLDFLRICVIATEFELVMPFV 123 Query: 126 QTFHDLGYETTINIMALSNVMENELLEAF-EQIREXXXXXXXXXXXXGSLDHNDIHYLVD 184 + G E +I ++ S++ E ++L A ++ R+ G+ D V+ Sbjct: 124 ERAQSKGLEVSIQLVK-SHLFEPDVLAAAGKRARDVGVRIVYVVDTTGTFLPEDARRYVE 182 Query: 185 KFQTHLPNKRLGVHTHNNLQLAFSNTLLASEKGVELLDASCYGMGRAAGNCPTELLVTHL 244 + + +G H HNNL +A +NTL A + G + LD + G GR AGNC E LV L Sbjct: 183 ALRG-ASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLVAAL 241 Query: 245 KN 246 + Sbjct: 242 QR 243 >Rv3469c [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 336 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 62/242 (25%), Positives = 118/242 (48%), Gaps = 11/242 (4%) Query: 8 IVDCTIRDGGLVNNWDFSVEFVQNLYAGLNEAGVDYMEIGYKNSPKLLKGAEGAGPWRFL 67 ++D T+RDG N+ ++ + V+ + L+ AG+ Y+EIG+ + + A GP Sbjct: 12 VLDTTVRDGSYAVNFQYTDDDVRRIVGDLDAAGIPYIEIGHGVT---IGAAAAQGPAAHT 68 Query: 68 NDDFLRKVIPQKGHTKLSALV--DIGRVDENDILPRSESMLDLIRVACYSKDVDKALQLV 125 ++++ R + +L A++ + R++ D+ + LD +R+ + + + + V Sbjct: 69 DEEYFRAARSVVRNARLGAVIVPALARIETVDL---AGDYLDFLRICVIATEFELVMPFV 125 Query: 126 QTFHDLGYETTINIMALSNVMENELLEAF-EQIREXXXXXXXXXXXXGSLDHNDIHYLVD 184 + G E +I ++ S++ E ++L A ++ R+ G+ D V+ Sbjct: 126 ERAQSKGLEVSIQLVK-SHLFEPDVLAAAGKRARDVGVRIVYVVDTTGTFLPEDARRYVE 184 Query: 185 KFQTHLPNKRLGVHTHNNLQLAFSNTLLASEKGVELLDASCYGMGRAAGNCPTELLVTHL 244 + + +G H HNNL +A +NTL A + G + LD + G GR AGNC E LV L Sbjct: 185 ALRG-ASDVSVGFHGHNNLAMAVANTLEAFDAGADFLDGTLMGFGRGAGNCQIECLVAAL 243 Query: 245 KN 246 + Sbjct: 244 QR 245 >ZmhpE [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 337 Score = 65.5 bits (158), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 67/274 (24%), Positives = 130/274 (47%), Gaps = 9/274 (3%) Query: 1 VKSNKSKIVDCTIRDGGLVNNWDFSVEFVQNLYAGLNEAGVDYMEIGYKNSPKLLKGAEG 60 + K I D T+RDG +S+E V+ + L++A VD +E+ + + + G Sbjct: 1 MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYG 60 Query: 61 AGPWRFLNDDFLRKVIPQKGHTKLSALVDIGRVDENDILPRSESMLDLIRVACYSKDVDK 120 G L +++ H K++ L+ G +D+ ++ ++RVA + + D Sbjct: 61 FGAHSDL--EWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADV 118 Query: 121 ALQLVQTFHDLGYETTINIMALSNVMENELLEAFEQIREXXXXXXXXXX-XXGSLDHNDI 179 + Q +Q +LG +T + + +S++ E L ++ E G+++ +DI Sbjct: 119 SAQHIQYARELGMDT-VGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDI 177 Query: 180 HYLVDKFQTHL-PNKRLGVHTHNNLQLAFSNTLLASEKGVELLDASCYGMGRAAGNCPTE 238 + L P + G+H H+NL L +N++ A E+G + +DAS GMG AGN P E Sbjct: 178 RDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLE 237 Query: 239 LLVTHLKNTRYT----LRPVLDILERLMIPLREK 268 + + + + L ++D + L+ PL+++ Sbjct: 238 VFIAAVDKLGWQHGADLYALMDAADDLVRPLQDR 271 >mhpE [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Length = 337 Score = 65.5 bits (158), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 67/274 (24%), Positives = 129/274 (47%), Gaps = 9/274 (3%) Query: 1 VKSNKSKIVDCTIRDGGLVNNWDFSVEFVQNLYAGLNEAGVDYMEIGYKNSPKLLKGAEG 60 + K I D T+RDG +S+E V+ + L++A VD +E+ + + + G Sbjct: 1 MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYG 60 Query: 61 AGPWRFLNDDFLRKVIPQKGHTKLSALVDIGRVDENDILPRSESMLDLIRVACYSKDVDK 120 G L +++ H K++ L+ G +D+ ++ ++RVA + + D Sbjct: 61 FGAHSDL--EWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADV 118 Query: 121 ALQLVQTFHDLGYETTINIMALSNVMENELLEAFEQIREXXXXXXXXXX-XXGSLDHNDI 179 + Q +Q +LG +T + + +S++ E L ++ E G+++ +DI Sbjct: 119 SAQHIQYARELGMDT-VGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDI 177 Query: 180 HYLVDKFQTHL-PNKRLGVHTHNNLQLAFSNTLLASEKGVELLDASCYGMGRAAGNCPTE 238 + L P + G+H H+NL L +N++ A E+G + +DAS GMG AGN P E Sbjct: 178 RDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLE 237 Query: 239 LLVTHLKNTRYT----LRPVLDILERLMIPLREK 268 + + + L ++D + L+ PL+++ Sbjct: 238 VFIAAADKLGWQHGTDLYALMDAADDLVRPLQDR 271 Score = 65.1 bits (157), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 67/274 (24%), Positives = 130/274 (47%), Gaps = 9/274 (3%) Query: 1 VKSNKSKIVDCTIRDGGLVNNWDFSVEFVQNLYAGLNEAGVDYMEIGYKNSPKLLKGAEG 60 + K I D T+RDG +S+E V+ + L++A VD +E+ + + + G Sbjct: 1 MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSIEVAHGDGLQGSSFNYG 60 Query: 61 AGPWRFLNDDFLRKVIPQKGHTKLSALVDIGRVDENDILPRSESMLDLIRVACYSKDVDK 120 G L +++ H K++ L+ G +D+ ++ ++RVA + + D Sbjct: 61 FGAHSDL--EWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQAGARVVRVATHCTEADV 118 Query: 121 ALQLVQTFHDLGYETTINIMALSNVMENELLEAFEQIREXXXXXXXXXX-XXGSLDHNDI 179 + Q +Q +LG +T + + +S++ E L ++ E G+++ +DI Sbjct: 119 SAQHIQYARELGMDT-VGFLMMSHMTTPENLAKQAKLMEGYGATCIYVVDSGGAMNMSDI 177 Query: 180 HYLVDKFQTHL-PNKRLGVHTHNNLQLAFSNTLLASEKGVELLDASCYGMGRAAGNCPTE 238 + L P + G+H H+NL L +N++ A E+G + +DAS GMG AGN P E Sbjct: 178 RDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCDRIDASLAGMGAGAGNAPLE 237 Query: 239 LLVTHLKNTRYT----LRPVLDILERLMIPLREK 268 + + + + L ++D + L+ PL+++ Sbjct: 238 VFIAAVDKLGWQHGADLYALMDAADDLVRPLQDR 271 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,877,566 Number of extensions: 940501 Number of successful extensions: 8025 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 8011 Number of HSP's successfully gapped: 13 Length of query: 319 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 213 Effective length of database: 26,153,027 Effective search space: 5570594751 Effective search space used: 5570594751 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)