BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0075.p (360 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3057 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 415 e-116 BS_leuB [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 400 e-111 CAC3171 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 385 e-107 XF2372 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 371 e-102 RSc1988 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase 371 e-102 >BH3057 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 363 Score = 415 bits (1067), Expect = e-116, Method: Compositional matrix adjust. Identities = 207/357 (57%), Positives = 264/357 (73%), Gaps = 1/357 (0%) Query: 5 KKIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEI 64 K+IAV+ GDGIGPEV A++VL+ + FG+ F ++AL GG AIDE GTPLPE+TL++ Sbjct: 3 KQIAVLPGDGIGPEVTDAAIEVLQAVADRFGHTFSYKKALLGGCAIDEVGTPLPEETLDV 62 Query: 65 CRSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKP 124 CR AD +LLGAVGGPKWD P LRPE GLLG+RK L LF+NLRP V+D L DASTLK Sbjct: 63 CRHADGILLGAVGGPKWDTLPGHLRPEKGLLGLRKGLNLFANLRPVTVYDSLADASTLKN 122 Query: 125 EVLEGTDLMVVRELTGGIYFGD-KLRRQGEFGEEAVDTCVYNVTEVERIVRQAFEIAGKR 183 +V++G DL++VRELTGG+YFG+ + RR EE VDT +Y E+ RI+R+AFE+A R Sbjct: 123 DVIDGVDLLIVRELTGGLYFGEPRERRGEGETEEVVDTLLYTRGEMRRIIRKAFELAMVR 182 Query: 184 RNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVIVTE 243 + SVDKANVLE+SR+WREV N +A E+P+V LEH+LVDN AMQL+RRP FDV+VTE Sbjct: 183 NKHVTSVDKANVLESSRMWREVANEVAQEFPEVTLEHMLVDNAAMQLIRRPKQFDVLVTE 242 Query: 244 NMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATILSLA 303 N+FGDILSDEA+M+TGS+GML SASL GLYEP+HGSAPDIAG+G+ANP+ATI S A Sbjct: 243 NLFGDILSDEASMVTGSLGMLPSASLTSDGPGLYEPIHGSAPDIAGKGVANPLATIASCA 302 Query: 304 LMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLIVAAIRKA 360 +M + +FG L G+RT+DIA ++ ST + D +V AI+ + Sbjct: 303 MMLKYSFGLHEEAKTIEDAIEAVLKQGYRTADIAKPGEESSSTKAITDAVVEAIQAS 359 >BS_leuB [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 365 Score = 400 bits (1029), Expect = e-111, Method: Compositional matrix adjust. Identities = 197/346 (56%), Positives = 245/346 (70%), Gaps = 4/346 (1%) Query: 5 KKIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEI 64 K+IA++ GDGIGPEV+ A VLK E F + FE E L GG AIDE PLPE+T+ Sbjct: 3 KRIALLPGDGIGPEVLESATDVLKSVAERFNHEFEFEYGLIGGAAIDEHHNPLPEETVAA 62 Query: 65 CRSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKP 124 C++ADA+LLGAVGGPKWD N ELRPE GLL IRK L LF+NLRP VF+ L D S LK Sbjct: 63 CKNADAILLGAVGGPKWDQNLSELRPEKGLLSIRKQLDLFANLRPVKVFESLSDRSPLKK 122 Query: 125 EVLEGTDLMVVRELTGGIYFGDKLRR--QGEFGEEAVDTCVYNVTEVERIVRQAFEIAGK 182 E ++ D ++VRELTGG+YFG +R E +EAVDT Y TE+ER++R+ F++A Sbjct: 123 EYIDNVDFVIVRELTGGLYFGQPSKRYVNTEGEQEAVDTLFYKRTEIERVIREGFKMAAA 182 Query: 183 RRNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVIVT 242 R+ K+ SVDKANVLE+SRLWREV +A E+P V+LEH+LVDN AMQL+ P+ FDV+VT Sbjct: 183 RKGKVTSVDKANVLESSRLWREVAEDVAQEFPDVKLEHMLVDNAAMQLIYAPNQFDVVVT 242 Query: 243 ENMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATILSL 302 ENMFGDILSDEA+MLTGS+GML SASL L+EPVHGSAPDIAG+G+ANP A ILS Sbjct: 243 ENMFGDILSDEASMLTGSLGMLPSASLSSSGLHLFEPVHGSAPDIAGKGMANPFAAILSA 302 Query: 303 ALMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQ 348 A++ R +FG L +G RT D+A +S+ S+ Q Sbjct: 303 AMLLRTSFGLEEEAKAVEDAVNKVLASGKRTRDLA--RSEEFSSTQ 346 >CAC3171 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 357 Score = 385 bits (989), Expect = e-107, Method: Compositional matrix adjust. Identities = 201/353 (56%), Positives = 253/353 (71%), Gaps = 1/353 (0%) Query: 6 KIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEIC 65 K+AVI GDGIG E+V EALKVL+K + F + GG AIDE G PLP +TLEIC Sbjct: 5 KVAVIPGDGIGVEIVGEALKVLEKVGAKYDTKFNFTEVKAGGCAIDEFGVPLPNETLEIC 64 Query: 66 RSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKPE 125 +++DAVLLGAVGGPKWD P E RPE L+G+R LGL++NLRPA V+D LK AS LK E Sbjct: 65 KNSDAVLLGAVGGPKWDTLPGEKRPEKALMGLRGGLGLYANLRPAKVYDILKSASPLKEE 124 Query: 126 VL-EGTDLMVVRELTGGIYFGDKLRRQGEFGEEAVDTCVYNVTEVERIVRQAFEIAGKRR 184 ++ +G DL+VVRELTGGIYFG++ R A DT YNV E++RI AF+ A KR Sbjct: 125 IINKGVDLLVVRELTGGIYFGERGRDIQNGINSAYDTERYNVEEIKRIAHVAFKAALKRN 184 Query: 185 NKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVIVTEN 244 K+ SVDKAN+LE+SRLWRE V +A EYP+VEL ++ VDN AMQL+R PS FDVIVT N Sbjct: 185 KKVTSVDKANILESSRLWRETVTEVAKEYPEVELNYLYVDNAAMQLVREPSQFDVIVTSN 244 Query: 245 MFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATILSLAL 304 +FGDIL+DEA+M+TGSIGML SASL ++G+YEP+HGSAPDIAGQ LANP+A ILS+A+ Sbjct: 245 IFGDILTDEASMVTGSIGMLPSASLRNDTFGMYEPIHGSAPDIAGQDLANPLAQILSVAM 304 Query: 305 MFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLIVAAI 357 M +F L++G+RT DI + SK + T +MG L++A + Sbjct: 305 MLEYSFNMTEAARDVEDAVEKVLNSGYRTGDIYTEGSKKVGTKEMGKLVLAEL 357 >XF2372 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 357 Score = 371 bits (952), Expect = e-102, Method: Compositional matrix adjust. Identities = 192/357 (53%), Positives = 244/357 (68%), Gaps = 6/357 (1%) Query: 5 KKIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEI 64 K+I ++ GDGIG E+VAEA+KVL+ GG A ++ G+PL ++TL Sbjct: 3 KQILILPGDGIGHEIVAEAVKVLEHVNVRHTLNLRLSFDALGGAAYEKYGSPLADETLAR 62 Query: 65 CRSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKP 124 R ADAVLLGAVGGP+WD LRPE GLL IR LGLF+NLRPA+++ L DASTLKP Sbjct: 63 ARDADAVLLGAVGGPQWDTIDPALRPERGLLKIRAQLGLFANLRPALLYPQLADASTLKP 122 Query: 125 EVLEGTDLMVVRELTGGIYFGD----KLRRQGEFGEEAVDTCVYNVTEVERIVRQAFEIA 180 EV+ G D+++VRELTGG+YFG ++ + GE EA DT Y +E+ RI++ FE+A Sbjct: 123 EVVAGLDILIVRELTGGLYFGQPRGSRISKNGE--REAFDTLPYCESEIRRILKVGFEMA 180 Query: 181 GKRRNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDVI 240 R KL SVDKANVL +S+LWR V +A +YP V L H+ VDN AMQL+R P FDVI Sbjct: 181 RLRGKKLCSVDKANVLASSQLWRTVAGEMAKDYPDVTLSHMYVDNAAMQLVRHPKQFDVI 240 Query: 241 VTENMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATIL 300 VTENMFGDILSD+A+MLTGSIGML SASL + G+YEP HGSAPDIAGQG+ANP+ATIL Sbjct: 241 VTENMFGDILSDQASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGVANPLATIL 300 Query: 301 SLALMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLIVAAI 357 S+A++ R + G+ LD+G RT+DI + + T MGD +VAA+ Sbjct: 301 SVAMLLRYSMGHIKTADAIEHAVGVVLDSGLRTADIWSEGMTKVGTVAMGDAVVAAL 357 >RSc1988 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Length = 363 Score = 371 bits (952), Expect = e-102, Method: Compositional matrix adjust. Identities = 193/358 (53%), Positives = 241/358 (67%), Gaps = 10/358 (2%) Query: 6 KIAVIAGDGIGPEVVAEALKVLKKTEELFGYAFETEQALFGGIAIDERGTPLPEDTLEIC 65 KIAV+ GDGIG E+VAEA+KVLK G FE E A GG + +G PLPEDTL++ Sbjct: 10 KIAVLPGDGIGTEIVAEAVKVLKT----LGETFEMETAPVGGAGYEAKGHPLPEDTLKLA 65 Query: 66 RSADAVLLGAVGGPKWDNNPKELRPETGLLGIRKALGLFSNLRPAVVFDCLKDASTLKPE 125 + ADA+L GAVG K+D P+ELRPE +LG+RK L LF+N RPA+ + L AS++KPE Sbjct: 66 KEADAILFGAVGDWKYDTLPRELRPEQAILGLRKHLQLFANFRPAICYPELAGASSMKPE 125 Query: 126 VLEGTDLMVVRELTGGIYFGD----KLRRQGEFG--EEAVDTCVYNVTEVERIVRQAFEI 179 ++ G D+++VRELTG IYFG + G F E DT Y+ E+ RI AF+ Sbjct: 126 IVAGLDILIVRELTGDIYFGQPRGVRAAPDGLFAGAREGFDTMRYSEPEIRRIAHVAFQA 185 Query: 180 AGKRRNKLASVDKANVLETSRLWREVVNRIAPEYPQVELEHVLVDNCAMQLLRRPSSFDV 239 A KR KL SVDKANVLET + W+++V + EYP+VEL H+ VDN AMQL++ P +FDV Sbjct: 186 AAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAPKNFDV 245 Query: 240 IVTENMFGDILSDEAAMLTGSIGMLASASLGEGSYGLYEPVHGSAPDIAGQGLANPIATI 299 +VT NMFGDILSDEAAMLTGSIGML SASL + GLYEP HGSAPDIAG+G+ANP+ATI Sbjct: 246 VVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATI 305 Query: 300 LSLALMFRLTFGYXXXXXXXXXXXXXXLDAGHRTSDIAVDKSKALSTAQMGDLIVAAI 357 LS A+M R T G L G+RT DI K + T +MGD +VAA+ Sbjct: 306 LSAAMMLRYTLGKAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAAL 363 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,131,190 Number of extensions: 1028460 Number of successful extensions: 3461 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 3247 Number of HSP's successfully gapped: 56 Length of query: 360 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 252 Effective length of database: 25,922,359 Effective search space: 6532434468 Effective search space used: 6532434468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)