BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0060.p (283 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0105 [S] COG0586 Uncharacterized membrane-associated protein 164 2e-40 TP0323 [R] COG1079 Uncharacterized ABC-type transport system, pe... 162 5e-40 mll4174 [R] COG1079 Uncharacterized ABC-type transport system, p... 139 7e-33 mlr3157 [R] COG1079 Uncharacterized ABC-type transport system, p... 135 6e-32 SMc04125 [R] COG1079 Uncharacterized ABC-type transport system, ... 132 7e-31 >STM0105 [S] COG0586 Uncharacterized membrane-associated protein Length = 255 Score = 164 bits (414), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 0/285 (0%), Positives = 0/285 (0%), Gaps = 11/285 (3%) Query: 1 VNIGLEGLMMXXXXXXXXXXXXXQDAGMGDWAPWIGVLCAMVVGVLGALIHAVASITFKA 60 +G + + +H + + Sbjct: 43 AGLGALIGSGELNFWHAWLVGIIGCL-------MGDWISFWLGWRFKKPLHRWSFMKKNK 95 Query: 61 DQTISGTVINFLAAGSTLYVVKLIFEGAGETPLLDGFNKVAIPGLSKIPVIGEGIFNSYP 120 + T+ V + + P++ G + + +IG ++ + Sbjct: 96 SLLDKTEHALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFIGPNLIGCLLWPPFY 155 Query: 121 TTXXXXXXXXXXXXXXXKTPFGLRLRSVGEHPSAADTLGVNVNRMRYIGVMLSXXXXXXX 180 ++ L + Sbjct: 156 FLPGILAGAAIDIPSDMQSGDFKWLLLATALLLWVGGWLCWRLWRSGKAAVDRLTAYFPR 215 Query: 181 XXXXXXXXXXXFAHNTVSGQGFIAIAAMIFGKWNPLGAFGAAVFFGLSQAIRNYVQLFEW 240 Sbjct: 216 - 274 Query: 241 SKDIP---QEFIFMIPYVLTIIVLVSAVGRSSAPKALGEPYDPSK 282 Sbjct: 275 319 >TP0323 [R] COG1079 Uncharacterized ABC-type transport system, permease component Length = 316 Score = 162 bits (410), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 95/284 (33%), Positives = 141/284 (49%), Gaps = 3/284 (1%) Query: 1 VNIGLEGLMMXXXXXXXXXXXXXQDAGMGDWAPWIGVLCAMVVGVLGALIHAVASITFKA 60 +NI LEGLMM + + APWI + M V AL +A S+ + Sbjct: 34 INIALEGLMMFGAFSTATVTVLCEPYTIA--APWIALGVGMAVAASVALFYAYLSVRLCS 91 Query: 61 DQTISGTVINFLAAGSTLYVVKLIFEGAGETPLLDGFNKVAIPGLSKIPVIGEGIF-NSY 119 DQ I+GT IN A G T++ ++IF G G K + S+IPV+G +F ++Y Sbjct: 92 DQIIAGTAINLCATGMTVFFAQVIFGQQGTQAYSRGLVKTSYGFFSRIPVLGPMVFTHTY 151 Query: 120 PTTXXXXXXXXXXXXXXXKTPFGLRLRSVGEHPSAADTLGVNVNRMRYIGVMLSXXXXXX 179 PT +TPFG+ +R+ G+ P A D G++V R+R V++S Sbjct: 152 PTVYLGFVLVALAWYVLYRTPFGVHVRATGDQPYAVDGAGLSVFRLRSAAVVISGLCAGL 211 Query: 180 XXXXXXXXXXXXFAHNTVSGQGFIAIAAMIFGKWNPLGAFGAAVFFGLSQAIRNYVQLFE 239 + + G GFIA+AA+I G+W+P G +V FG SQ + Y E Sbjct: 212 GGGVLILTQDIQYTVYSTHGTGFIALAALISGRWHPFGVLVTSVLFGFSQILNVYATSVE 271 Query: 240 WSKDIPQEFIFMIPYVLTIIVLVSAVGRSSAPKALGEPYDPSKR 283 K +P E +PY LT++VL+ GR AP+A+G+PYD ++R Sbjct: 272 LLKHLPIELFSALPYALTVVVLLLFGGRGEAPRAIGQPYDRARR 315 >mll4174 [R] COG1079 Uncharacterized ABC-type transport system, permease component Length = 314 Score = 139 bits (349), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 82/283 (28%), Positives = 136/283 (48%), Gaps = 8/283 (2%) Query: 1 VNIGLEGLMMXXXXXXXXXXXXXQDAGMGDWAPWIGVLCAMVVGVLGALIHAVASITFKA 60 +N+G+EG+M+ W+GVL A++ G+L +HA+ ++ Sbjct: 40 LNLGIEGIMLLCAMTGFTATSLSGSL-------WLGVLVAVLTGMLMGALHALFTVALGL 92 Query: 61 DQTISGTVINFLAAGSTLYVVKLIFEGAGETPLLDGFNKVAIPGLSKIPVIGEGIFNSYP 120 Q + G + ++G ++ +LIF P + GF + IP LS IPV+G +FN + Sbjct: 93 SQHVCGIGVTLFSSGLAYFLYRLIFGQQSVPPAIKGFQTLPIPVLSDIPVLGPAVFNQFA 152 Query: 121 TTXXXXXXXXXXXXXXXKTPFGLRLRSVGEHPSAADTLGVNVNRMRYIGVMLSXXXXXXX 180 +TP+GL +R VGE+P AAD+ GV+V R+ V+L Sbjct: 153 LVYMAIIAIPLAAFVLYRTPWGLSVRMVGENPRAADSAGVSVIATRFQAVILGGALMGLA 212 Query: 181 XXXXXXXXXXXFAHNTVSGQGFIAIAAMIFGKWNPLGAFGAAVFFGLSQAIRNYVQLFEW 240 F VSG+G++AIA ++FG+W+P + GAA+ F A++ +Q Sbjct: 213 GAFLSMAQFNAFTFGVVSGRGWVAIALVVFGRWDPWRSAGAALLFAFVDALQLRMQA-SG 271 Query: 241 SKDIPQEFIFMIPYVLTIIVLVSAVGRSSAPKALGEPYDPSKR 283 IP E M+P++ TI+ + + AP AL +P+ +R Sbjct: 272 LGHIPYEAFLMLPFIFTIVAMAVMSRNAVAPSALLKPFRKEER 314 >mlr3157 [R] COG1079 Uncharacterized ABC-type transport system, permease component Length = 323 Score = 135 bits (341), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 92/293 (31%), Positives = 146/293 (49%), Gaps = 19/293 (6%) Query: 2 NIGLEGLMMXXXXXXXXXXXXXQDAGMGDWAPWIGVLCAMVVGVLGALIHAVASITFKAD 61 +IGLEG M+ A +IG+ A+++ V A++H ASIT + + Sbjct: 38 DIGLEGKMLVGAFAGAAAASVFHSA-------FIGLGMAILISVAFAMVHGFASITHRGN 90 Query: 62 QTISGTVINFLAAGSTLYVVKLIFEGAGETPLL---DGFNKVAIPG---LSKIPVIG--- 112 Q +SG INF+AAGST+ + + F+ G TP L + F+ + PG + +P+IG Sbjct: 91 QIVSGVAINFIAAGSTIILGQAWFQQGGRTPALQPGERFDAIVWPGAEAVRDVPIIGPIY 150 Query: 113 -EGIFNSYPTTXXXXXXXXXXXXXXXKTPFGLRLRSVGEHPSAADTLGVNVNRMRYIGVM 171 E I +T FGLRLR+VGE+P+A DT G++V +RY ++ Sbjct: 151 AELISGHSLLVYLAFLMVPFTWWVLFRTRFGLRLRAVGENPAAVDTAGISVAWLRYRALI 210 Query: 172 LSXXXXXXXXXXXXXXXXXXFAHNTVSGQGFIAIAAMIFGKWNPLGAFGAAVFFGL--SQ 229 + F + +G+G+IA+AA+IF KW P+ A A + FG + Sbjct: 211 CTGILTGVAGAYLSMVQNGGFVKDMTAGKGYIALAALIFAKWKPVNAMFACLLFGFLDAA 270 Query: 230 AIRNYVQLFEWSKDIPQEFIFMIPYVLTIIVLVSAVGRSSAPKALGEPYDPSK 282 AIR +P +F+ +PY+LT+I+L +G++ P+A G PY + Sbjct: 271 AIRLQGSPLPIIGKVPVQFMQALPYILTVILLAGFIGKAIPPRAGGVPYVKER 323 >SMc04125 [R] COG1079 Uncharacterized ABC-type transport system, permease component Length = 323 Score = 132 bits (332), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 93/293 (31%), Positives = 141/293 (48%), Gaps = 19/293 (6%) Query: 2 NIGLEGLMMXXXXXXXXXXXXXQDAGMGDWAPWIGVLCAMVVGVLGALIHAVASITFKAD 61 +IGLEG M+ Q W+G+ A++V V +L+H ASIT + + Sbjct: 38 DIGLEGKMLGAAFAAGAAAAVTQSV-------WVGLGAAILVSVALSLVHGYASITQRGN 90 Query: 62 QTISGTVINFLAAGSTLYVVKLIFEGAGETP-LLDG--FNKVAIPG---LSKIPVIG--- 112 Q +SG INF+ AGST+ + + F G TP L +G F + P + +IPV+G Sbjct: 91 QIVSGVAINFIVAGSTVILGEAWFRQGGRTPALAEGARFQTINFPDADMIREIPVLGPVY 150 Query: 113 -EGIFNSYPTTXXXXXXXXXXXXXXXKTPFGLRLRSVGEHPSAADTLGVNVNRMRYIGVM 171 + + + T +T FGLRLR+VGE+P A DT G++V +RY V+ Sbjct: 151 ADLLSGHFLLTYLAFAMVPISWWILYRTRFGLRLRAVGENPGAVDTAGISVIWLRYRAVI 210 Query: 172 LSXXXXXXXXXXXXXXXXXXFAHNTVSGQGFIAIAAMIFGKWNPLGAFGAAVFFGL--SQ 229 F +G+G+IA+AA+IF KW PL A + FG + Sbjct: 211 CCGILCGFAGAYLSLAMTAGFVKGMTAGKGYIALAALIFAKWRPLNIMFACLLFGFLDAL 270 Query: 230 AIRNYVQLFEWSKDIPQEFIFMIPYVLTIIVLVSAVGRSSAPKALGEPYDPSK 282 AIR +P + + +PY+LT+I+L +G++ PKA G PY + Sbjct: 271 AIRLQGTPLPLVGQVPVQLMQALPYILTVILLAGFIGKAIPPKAGGVPYVKER 323 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.140 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,278,574 Number of extensions: 669530 Number of successful extensions: 1482 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 1427 Number of HSP's successfully gapped: 25 Length of query: 283 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 178 Effective length of database: 26,268,361 Effective search space: 4675768258 Effective search space used: 4675768258 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 115 (48.9 bits)