BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0053.p (165 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all4623 [J] COG0290 Translation initiation factor 3 (IF-3) 167 5e-42 AGc4363 [S] COG3791 Uncharacterized conserved protein 163 1e-40 all2368 [J] COG0290 Translation initiation factor 3 (IF-3) 90 2e-18 >all4623 [J] COG0290 Translation initiation factor 3 (IF-3) Length = 204 Score = 167 bits (424), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 77/162 (47%), Positives = 117/162 (72%) Query: 2 INDEIRAKEVRLVGAEGEQIGIKPIREALQMAIDLNLDLVNVAPQAKPPVCRIMDYGKFR 61 IN+ IR ++R++ +G Q+GI +EALQ+A + LDLV ++ +A PPVCRIMDYGK++ Sbjct: 42 INERIRFPKIRVIDTDGSQLGILTPQEALQLAEEKELDLVLLSDKADPPVCRIMDYGKYK 101 Query: 62 YETQKKEKEARKNQKIVDIKEVWFRANIEEHDYQTKFRNVIKFLGEGDKVKCSVRFRGRE 121 +E +KK +EARK Q D+KEV R IEEHDY + + +FL +GDKVK +V FRGRE Sbjct: 102 FEQEKKAREARKKQHTADVKEVKMRYKIEEHDYNVRVKQAERFLKDGDKVKATVMFRGRE 161 Query: 122 ITHASIGQKILERVKSEVEDISVVERQPKLEGRSMIMILAPK 163 I H+ + + +L+R+ +++E V++ PK EGR+M+M+++PK Sbjct: 162 IQHSDLAEDLLKRMATDLEPFGEVQQAPKKEGRNMMMLISPK 203 >AGc4363 [S] COG3791 Uncharacterized conserved protein Length = 140 Score = 163 bits (412), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 73/139 (52%), Positives = 103/139 (74%) Query: 25 PIREALQMAIDLNLDLVNVAPQAKPPVCRIMDYGKFRYETQKKEKEARKNQKIVDIKEVW 84 P +AL+MA + LDLV ++P A+PPVC+I+D GK +Y TQKK EARK QKIV++KE+ Sbjct: 2 PTDQALKMAEEAGLDLVEISPNAEPPVCKILDLGKLKYSTQKKAAEARKKQKIVEVKEIK 61 Query: 85 FRANIEEHDYQTKFRNVIKFLGEGDKVKCSVRFRGREITHASIGQKILERVKSEVEDISV 144 R NI+ HDY K + + +F EGDKVK +++FRGRE+ H +G K+L +VK + + I+ Sbjct: 62 MRPNIDTHDYDVKMKAMNRFFDEGDKVKVTLKFRGREMAHQELGMKLLLQVKEDTQAIAK 121 Query: 145 VERQPKLEGRSMIMILAPK 163 VE +PKLEGR M+M+LAP+ Sbjct: 122 VEAEPKLEGRQMMMVLAPR 140 >all2368 [J] COG0290 Translation initiation factor 3 (IF-3) Length = 144 Score = 89.7 bits (221), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 50/150 (33%), Positives = 86/150 (57%), Gaps = 7/150 (4%) Query: 13 LVGAEGEQIGIKPIREALQMAIDLNLDLVNVAPQAKPPVCRIMDYGKFRYETQKKEKEAR 72 ++ E G+ EALQ+A + LDLV V+ PV +I++YGK +Y+ +K++ ++ Sbjct: 1 MIDHENNNRGLIDTYEALQLAQSVELDLVVVSQSKDTPVAKILNYGKLQYQKKKRQGQSA 60 Query: 73 KNQKIVDIKEVWFRANIEEHDYQTKFRNVIKFLGEGDKVKCSVRFRGREITHASIGQKIL 132 + +KEV FR N+ DY + +++L +GD VK ++R RGRE + ++L Sbjct: 61 RP----TVKEVRFRPNVGAADYNLRIEQALQWLSKGDSVKFAIRLRGRENQYREQAGQML 116 Query: 133 ERVKSEVEDISVVERQPKLEGRSMIMILAP 162 ER+ V D+S V + L+ RS+I+ + P Sbjct: 117 ERI---VTDLSQVGKVQSLDKRSLIVQIMP 143 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,464,473 Number of extensions: 426190 Number of successful extensions: 2462 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 2461 Number of HSP's successfully gapped: 3 Length of query: 165 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 67 Effective length of database: 27,075,699 Effective search space: 1814071833 Effective search space used: 1814071833 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 112 (47.8 bits)