BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0046.p (205 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA0960 [QR] COG0500 SAM-dependent methyltransferases 105 4e-23 NMB0747 [QR] COG0500 SAM-dependent methyltransferases 105 4e-23 SA1582 [QR] COG0500 SAM-dependent methyltransferases 100 3e-21 lin1771 [QR] COG0500 SAM-dependent methyltransferases 90 2e-18 SP0652 [QR] COG0500 SAM-dependent methyltransferases 79 5e-15 >NMA0960 [QR] COG0500 SAM-dependent methyltransferases Length = 521 Score = 105 bits (263), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 12/199 (6%), Positives = 29/199 (14%), Gaps = 15/199 (7%) Query: 1 MGFMSVLSFAHKLTEGRLQPGGRAIDATVGTGADTLFLAKAAGPRGGVYGFDIQPXXXXX 60 M + +F + G L+ + + + P +I Sbjct: 1 MTYWVPETFIYVCVAGGLKKMKTGFFTYLIYVLLVMLIIPGMAPGMASAIGEIAG----- 55 Query: 61 XXXXXXXXXXXXXXXXSPVTLLLQSHAAMAEAVPPDWRGTVSAVMFNLGYLPSGDADKTI 120 + P+ D Sbjct: 56 -DSGSSDTGDSGDSGTGDPGTGADDSGTTDNPADD----PADDPADDPADDPAD--DPAD 108 Query: 121 ITEPSSTXXXXXXXXXXXRPGGIITAVLYPGHEGGDRXXXXXXXXXXGLPSRQAQAXXXX 180 + ST + + D + + Sbjct: 109 DSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTDDSTD 168 Query: 181 IVYRQLQRASAPYTLAVEK 199 ++ T Sbjct: 169 DSTD---DSTDDSTDDSTD 184 >NMB0747 [QR] COG0500 SAM-dependent methyltransferases Length = 188 Score = 105 bits (263), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 66/202 (32%), Positives = 89/202 (44%), Gaps = 15/202 (7%) Query: 1 MGFMSVLSFAHKLTEGRLQPGGRAIDATVGTGADTLFLAKAAGPRGGVYGFDIQPXXXXX 60 M ++L FAH L L GG A+D T G G DTLFLA+ AG RG V+ FDIQP Sbjct: 1 MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGIRGKVWAFDIQP----- 55 Query: 61 XXXXXXXXXXXXXXXXSPVTLLLQSHAAMAEAVPPDWRGTVSAVMFNLGYLPSGDADKTI 120 S V L+L H + + +P + A +FN G+LP G DK++ Sbjct: 56 -QALNNTRCRLQEAGYSNVRLILDGHENLKQYIPK----PLDAAIFNFGWLPGG--DKSL 108 Query: 121 ITEPSSTXXXXXXXXXXXRPGGIITAVLYPGHEGGDRXXXXXXXXXXGLPSRQAQAXXXX 180 T ++ + G++ AVLYPGHE G + LP Q Sbjct: 109 TTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQEQFAVLRYG 168 Query: 181 IVYRQLQRASAPYTLAVEKKKE 202 R + S PY L EK ++ Sbjct: 169 FTNR---KNSPPYLLVFEKLRQ 187 >SA1582 [QR] COG0500 SAM-dependent methyltransferases Length = 187 Score = 99.8 bits (247), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 64/200 (32%), Positives = 88/200 (44%), Gaps = 16/200 (8%) Query: 1 MGFMSVLSFAHKLTEGRLQPGGRAIDATVGTGADTLFLAKAAGPRGGVYGFDIQPXXXXX 60 M +L F+ L + + P +DAT G G DTLFLA+ P G VYGFDIQ Sbjct: 1 MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQV-PEGHVYGFDIQDLALEN 59 Query: 61 XXXXXXXXXXXXXXXXSPVTLLLQSHAAMAEAVPPDWRGTVSAVMFNLGYLPSGDADKTI 120 + V+L+ H + + +G + A +FNLGYLP G DK+I Sbjct: 60 TRDKVKDF--------NHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKG--DKSI 109 Query: 121 ITEPSSTXXXXXXXXXXXRPGGIITAVLYPGHEGGDRXXXXXXXXXXGLPSRQAQAXXXX 180 +T+P +T GII V+Y GH G L + AQ Sbjct: 110 VTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQV---- 165 Query: 181 IVYRQL-QRASAPYTLAVEK 199 + Y+ L QR AP+ A+EK Sbjct: 166 LQYQFLNQRNHAPFICAIEK 185 >lin1771 [QR] COG0500 SAM-dependent methyltransferases Length = 301 Score = 90.1 bits (222), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 9/202 (4%), Positives = 26/202 (12%), Gaps = 16/202 (7%) Query: 1 MGFMSVLSFAHKLTEGRLQPGGRAIDATVGTGADTLFLAKAAGPRGGVYGFDIQPXXXXX 60 M + ++ +G V Sbjct: 1 MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEG-----A 55 Query: 61 XXXXXXXXXXXXXXXXSPVTLLLQSHAAMAE-AVPPDWRGTVSAVMFNLGYLPSGDADKT 119 + L+ A+ + V V V+ + P + Sbjct: 56 YQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVEL-----VIFVVEGTRWTPDD--EMV 108 Query: 120 IITEPSSTXXXXXXXXXXXRPGGIITAVLYPGHEGGDRXXXXXXXXXXGLPSRQAQAXXX 179 + + + Sbjct: 109 LNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAI 168 Query: 180 XIVYRQLQRASAPYTLAVEKKK 201 + + + Sbjct: 169 VRKH---LPEATHHFPEDYITD 187 >SP0652 [QR] COG0500 SAM-dependent methyltransferases Length = 185 Score = 79.0 bits (193), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 60/194 (30%), Positives = 82/194 (42%), Gaps = 21/194 (10%) Query: 7 LSFAHKLTEGRLQPGGRAIDATVGTGADTLFLAKAAGPRGGVYGFDIQPXXXXXXXXXXX 66 L AH + +DAT+G G DTLFLAK A VY FDIQ Sbjct: 5 LEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQ---VYAFDIQKQALEKTQERLH 61 Query: 67 XXXXXXXXXXSPVTLLLQSHAAMAEAVPPDWRGTVSAVMFNLGYLPSGDADKTIITEPSS 126 + L+LQ H + + V G +FNLGYLP+ ADK++IT P + Sbjct: 62 QADL------TNAQLILQGHETLDQFVIKAKAG-----IFNLGYLPA--ADKSVITRPQT 108 Query: 127 TXXXXXXXXXXXRPGGIITAVLYPGHEGGDRXXXXXXXXXXGLPSRQAQAXXXXIVYRQL 186 T GG I ++Y GHEGGD L ++ A +YR L Sbjct: 109 TIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAA----IYRTL 164 Query: 187 QRASA-PYTLAVEK 199 + + P+ + +EK Sbjct: 165 NQVNNPPFLVMIEK 178 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,688,160 Number of extensions: 366904 Number of successful extensions: 601 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 586 Number of HSP's successfully gapped: 8 Length of query: 205 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 104 Effective length of database: 26,729,697 Effective search space: 2779888488 Effective search space used: 2779888488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 113 (48.1 bits)