BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0044.p (253 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydhS [G] COG1482 Phosphomannose isomerase 209 4e-54 SA2435 [G] COG1482 Phosphomannose isomerase 205 6e-53 BS_yjdE [G] COG1482 Phosphomannose isomerase 205 7e-53 BS_pmi [G] COG1482 Phosphomannose isomerase 189 2e-48 SA1945 [G] COG1482 Phosphomannose isomerase 172 5e-43 >BS_ydhS [G] COG1482 Phosphomannose isomerase Length = 534 Score = 209 bits (532), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 11/220 (5%), Positives = 33/220 (15%), Gaps = 8/220 (3%) Query: 5 YPLKFQPEFKERVWGGRAL-EKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQ 63 L ++ L + + + + + ++ Sbjct: 3 KIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPYSDEENSKMMLANIASIEI 62 Query: 64 IRLQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPK-GELGKTEMW 122 + + L + Q P E + L + Sbjct: 63 PPIYCT-YLEWLQ----KQEDSHLQRYGVKKETLHDRQFLPRILLGEYFRDQFLRLVDQA 117 Query: 123 YVLDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCA 182 + + L + + + P Sbjct: 118 RQQKFAVAVYESCQ-VTDLQITNAGVMLATNQDLPSETFDLAVIATGHVWPDEEEATRTY 176 Query: 183 GVVVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNV 222 + L G + + Sbjct: 177 FPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGS 216 >SA2435 [G] COG1482 Phosphomannose isomerase Length = 312 Score = 205 bits (521), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 103/216 (47%), Positives = 141/216 (65%), Gaps = 6/216 (2%) Query: 7 LKFQPEFKERVWGGRALEKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQIRL 66 L QP FK+R+WGG AL+ F ++P GE W I+ HPNG ++++NG LDQ+ Sbjct: 3 LFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGPNTIINGPYKDMTLDQLWS 62 Query: 67 QFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPKGELGKTEMWYVLD 126 Q E F + FPLL K+LD ND LSVQVHP DDY +GELGKTE WY+LD Sbjct: 63 QH-RELFDNDSR----DSFPLLTKVLDANDKLSVQVHPDDDYALKHEGELGKTECWYILD 117 Query: 127 AKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCAGVVV 186 A PGA+IIYG+ +++L + +++ + I V GD +Y+PAGTVHA+ +G+++ Sbjct: 118 AAPGAEIIYGVH-AHNKQTLIDMIDNHEFDSLFKRIPVKPGDFYYVPAGTVHAIGSGILI 176 Query: 187 AEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNV 222 E QQ+SDTTYRIYDYDR +G+ R+LH+E S +V Sbjct: 177 LETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDV 212 >BS_yjdE [G] COG1482 Phosphomannose isomerase Length = 315 Score = 205 bits (521), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 106/218 (48%), Positives = 138/218 (63%), Gaps = 7/218 (3%) Query: 6 PLKFQPEFKERVWGGRALEKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQIR 65 PL F+P FKER+WGG AL FG +P GE W A H NG S V NG G L ++ Sbjct: 5 PLFFKPVFKERIWGGTALADFGYTIPSQRTGECWAFAAHQNGQSVVQNGMYKGFTLSELW 64 Query: 66 LQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYEELPK-GELGKTEMWYV 124 H FG +G RFPLL K+LD + +LSVQVHP D+Y + + GELGKTE WY+ Sbjct: 65 EHHRH-LFGQL----EGDRFPLLTKILDADQDLSVQVHPNDEYANIHENGELGKTECWYI 119 Query: 125 LDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCAGV 184 +D + A+IIYG + T+E L +E G + +R + V GD FY+P+GTVHA+ G+ Sbjct: 120 IDCQKDAEIIYG-HNATTKEELTTMIERGEWDELLRRVKVKPGDFFYVPSGTVHAIGKGI 178 Query: 185 VVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNV 222 + E QQNSDTTYR+YDYDR +GK RELH++ S+ V Sbjct: 179 LALETQQNSDTTYRLYDYDRKDAEGKLRELHLKKSIEV 216 >BS_pmi [G] COG1482 Phosphomannose isomerase Length = 316 Score = 189 bits (481), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 103/219 (47%), Positives = 135/219 (61%), Gaps = 10/219 (4%) Query: 6 PLKFQPEFKERVWGGRAL-EKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQI 64 P+ P FKE++WGG AL ++FG +P GE W I+ HP G S+V NG G+ L I Sbjct: 5 PIFLTPVFKEKIWGGTALRDRFGYSIPSESTGECWAISAHPKGPSTVANGPYKGKTL--I 62 Query: 65 RLQFGH-EWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYE-ELPKGELGKTEMW 122 L H E FG +G RFPLL KLLD ++ S++VHP D Y E +GELGKTE W Sbjct: 63 ELWEEHREVFGGV----EGDRFPLLTKLLDVKEDTSIKVHPDDYYAGENEEGELGKTECW 118 Query: 123 YVLDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCA 182 Y++D K A+IIYG ++ L + SG +R I + GD +Y+P+GT+HALC Sbjct: 119 YIIDCKENAEIIYG-HTARSKTELVTMINSGDWEGLLRRIKIKPGDFYYVPSGTLHALCK 177 Query: 183 GVVVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLN 221 G +V E QQNSD TYR+YDYDR +G PRELH ++N Sbjct: 178 GALVLETQQNSDATYRVYDYDRLDSNGSPRELHFAKAVN 216 >SA1945 [G] COG1482 Phosphomannose isomerase Length = 312 Score = 172 bits (435), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 91/223 (40%), Positives = 135/223 (60%), Gaps = 7/223 (3%) Query: 6 PLKFQPEFKERVWGGRALEKFGLELPEGHIGEGWMIADHPNGTSSVLNGELAGQGLDQIR 65 PL QP K ++WGG+ L +FG +L GE W ++ HPNGTS ++NG GQ LD+I Sbjct: 2 PLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGTSEIINGPYQGQTLDRIW 61 Query: 66 LQFGHEWFGSKGSPKDGGRFPLLIKLLDCNDNLSVQVHPTDDYE-ELPKGELGKTEMWYV 124 + E FG S KD FPLL K++D ++LS+ VHP + Y E G+ GK+E WY+ Sbjct: 62 SE-HRELFGDFPS-KD---FPLLTKIVDARESLSIHVHPDNSYAYEHENGQYGKSECWYI 116 Query: 125 LDAKPGAKIIYGLKDGVTRESLREALESGTVMDTMREITVSAGDAFYIPAGTVHALCAGV 184 +DA+ A+I+ G +RE + ++ GT+ +R I V G+ ++IPAGTVH + +G+ Sbjct: 117 IDAEEDAEIVIGTL-AESREEVANHVQHGTIESILRYIKVKPGEFYFIPAGTVHTISSGI 175 Query: 185 VVAEIQQNSDTTYRIYDYDRPGLDGKPRELHIEDSLNVTAYEG 227 + E Q+SD TYR+YD++R R L+IE +L+V Y Sbjct: 176 LAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA 218 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,096,974 Number of extensions: 989006 Number of successful extensions: 1479 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1463 Number of HSP's successfully gapped: 5 Length of query: 253 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 149 Effective length of database: 26,383,695 Effective search space: 3931170555 Effective search space used: 3931170555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 114 (48.5 bits)