BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0042.p (320 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3301 [I] COG2267 Lysophospholipase 172 7e-43 mlr1612 [I] COG2267 Lysophospholipase 129 5e-30 SA1143 [I] COG2267 Lysophospholipase 129 7e-30 BMEI0552 [I] COG2267 Lysophospholipase 123 4e-28 AGc3857 [I] COG2267 Lysophospholipase 118 1e-26 >PA3301 [I] COG2267 Lysophospholipase Length = 316 Score = 172 bits (436), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 103/285 (36%), Positives = 145/285 (50%), Gaps = 3/285 (1%) Query: 25 PEAPVRGIVQIAHGMCEXXXXXXXXXXXXXXXXXXXXXNDHRGHGQTAGRVELLGDTGEN 84 P A G V ++HGM E D RGHG+TA EL + Sbjct: 22 PSATAVGAVMLSHGMAEHAGRYERLAAALNAAGYHFYAIDQRGHGRTAEADELGHFADQG 81 Query: 85 GFYWMRRNLLQLAGIARSRHEGLPIFLLAHSMGSFLAQKLMCEEGRDIYAGFILSGTN-G 143 G+ + +L L R +H LPIFLL HSMGS+++ + + G ILSG+N Sbjct: 82 GWGKVVGDLASLNHHIRQQHPELPIFLLGHSMGSYISMAYLLHHSCSL-QGAILSGSNYQ 140 Query: 144 PRTMLRLGESLAGLQFRLQGKRHRSVLLNGIVFGPYNRSFTPVRTAFDWLSSDTQEVDRF 203 P+ + R+ +A + QG +S L++ + FG +N++F P RTAFDWLS D QEVDR+ Sbjct: 141 PQALYRIARLIARFERWRQGPLGKSALIDFLSFGSFNKAFKPNRTAFDWLSRDPQEVDRY 200 Query: 204 IADPFCGAICTTXXXXXXXXXXXXIHSRETLVTLCRDKPVYLFAGAKDPVGMNGQGVLRL 263 +ADP CG C+ I L + D P+ + G +DPV G+ + L Sbjct: 201 VADPLCGFRCSNQLWVDLLGGLADITPPTHLRQIDADLPLLIIGGERDPVS-QGKRLGDL 259 Query: 264 AEIYREQGISDVEYRLYPEGRHEMLNEVNRDEVTADVLDWLVRHL 308 A+ R G+ V + YPE RHE+ NE NRD VT D++DWL + L Sbjct: 260 ADALRGAGLRQVTLKTYPEARHELFNESNRDAVTQDLIDWLEQAL 304 >mlr1612 [I] COG2267 Lysophospholipase Length = 309 Score = 129 bits (325), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 92/298 (30%), Positives = 134/298 (44%), Gaps = 11/298 (3%) Query: 11 PLGVDIHVYEWLPEPEAPVRGIVQIAHGMCEXXXXXXXXXXXXXXXXXXXXXNDHRGHGQ 70 P G D+++ + + E R +VQI HG+ E +DHRGHG Sbjct: 12 PTGADLNLL--VSQAEDEPRAVVQINHGLAEHAARYARFADFLSARGFHVYVHDHRGHGA 69 Query: 71 TAGRVELLGD-TGENGFYWMRRNLLQLAGIARSRHEGLPIFLLAHSMGSFLAQKLMCEEG 129 T LG E+G + ++ + + S GLP+ L HSMG+ +A + Sbjct: 70 TKAPDAPLGKFADEDGPAKVIADVDAIHDLIASECPGLPVILFGHSMGASVALNYLLGHS 129 Query: 130 RDIYAGFILSGTNGPRTMLRLGESLAGL----QFRLQGKRHRSVLLNGIVFGPYNRSFTP 185 I+A I NG + RLG+ G+ + RL G S LL + F + ++ Sbjct: 130 PRIHAAAIW---NGNFSQGRLGQVALGILAWERMRL-GSDVPSRLLPKLTFQAWGKAVPN 185 Query: 186 VRTAFDWLSSDTQEVDRFIADPFCGAICTTXXXXXXXXXXXXIHSRETLVTLCRDKPVYL 245 RT FDWLS D EV ++IADP CG + T + RD PV + Sbjct: 186 HRTLFDWLSRDEAEVAKYIADPLCGWDASVSMWRDVVSMAINGGKDATFAGVRRDLPVAV 245 Query: 246 FAGAKDPVGMNGQGVLRLAEIYREQGISDVEYRLYPEGRHEMLNEVNRDEVTADVLDW 303 G KDP G+G++ LA R G S++ ++Y + RHE LNE+NRD + D W Sbjct: 246 VGGEKDPASDYGKGIIHLANRMRGMGFSNLVSKVYADTRHESLNELNRDIIMNDFATW 303 >SA1143 [I] COG2267 Lysophospholipase Length = 304 Score = 129 bits (324), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 92/310 (29%), Positives = 142/310 (45%), Gaps = 18/310 (5%) Query: 1 MRENTFKMTDPLGVDIHVYEWLPEPEAPVRGIVQIAHGMCEXXXXXXXXXXXXXXXXXXX 60 M E FK+T +I V + ++ +GI+ I HGM E Sbjct: 1 MTEKQFKLTVQDNTNIEVKVNFTDVDS--KGIIHIFHGMAEHMERYDKLAHALSKHGFDV 58 Query: 61 XXNDHRGHGQTAGRVELLGDTGENGFYW-MRR---NLLQLAGIARSRHEGLPIFLLAHSM 116 ++HRGHG D G Y M+R + ++A R + P ++ HSM Sbjct: 59 IRHNHRGHGINI-------DESTRGHYDDMKRVIGDAFEVAQTVRGNVDK-PYIIIGHSM 110 Query: 117 GSFLAQKLMCEEGRDIYAGFILSGTNGPRTMLRLGESLAGLQF--RLQGKRHRSVLLNGI 174 GS +A +L E G ILSGT G ++ + ++ LQ ++ G R +N + Sbjct: 111 GSVIA-RLFVETYPQYVDGLILSGT-GMYSLWKGLPTVKVLQLITKIYGAEKRVEWVNQL 168 Query: 175 VFGPYNRSFTPVRTAFDWLSSDTQEVDRFIADPFCGAICTTXXXXXXXXXXXXIHSRETL 234 V +N+ P+RT DW+SS+ EVD FI DP+ G + + + Sbjct: 169 VSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYYMLHTSQLKNM 228 Query: 235 VTLCRDKPVYLFAGAKDPVGMNGQGVLRLAEIYREQGISDVEYRLYPEGRHEMLNEVNRD 294 L P+ L +G D +G G+G+L+LA IYR+ GI +V+ LY RHE+L E + D Sbjct: 229 KMLNHAMPILLVSGYDDSLGDYGKGILKLANIYRKAGIKNVKVNLYHHKRHEVLFEKDHD 288 Query: 295 EVTADVLDWL 304 ++ D+ WL Sbjct: 289 KIWEDLFKWL 298 >BMEI0552 [I] COG2267 Lysophospholipase Length = 314 Score = 123 bits (308), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 81/283 (28%), Positives = 118/283 (41%), Gaps = 1/283 (0%) Query: 31 GIVQIAHGMCEXXXXXXXXXXXXXXXXXXXXXNDHRGHGQTAGRVELLGDTG-ENGFYWM 89 G+VQ+ HG+ E DHRGHG G + G Sbjct: 29 GVVQLCHGLAEHSARYERFASALAGAGYHVYVQDHRGHGANITSHAPKGMFAPKQGHAVA 88 Query: 90 RRNLLQLAGIARSRHEGLPIFLLAHSMGSFLAQKLMCEEGRDIYAGFILSGTNGPRTMLR 149 +++ L + LP+ L HSMG +A + + + A I + Sbjct: 89 IKDVRTLNHYIHQTYADLPVVLFGHSMGGLIALNYVFDHADTVDAVAIWNSNFDGGAQSA 148 Query: 150 LGESLAGLQFRLQGKRHRSVLLNGIVFGPYNRSFTPVRTAFDWLSSDTQEVDRFIADPFC 209 +L ++ L+G S +L + F + RS RT FDWLS D EVD +IADP C Sbjct: 149 AALALLYMERMLKGSDVPSGILPRLTFRAWGRSIKGHRTLFDWLSHDPAEVDAYIADPLC 208 Query: 210 GAICTTXXXXXXXXXXXXIHSRETLVTLCRDKPVYLFAGAKDPVGMNGQGVLRLAEIYRE 269 G + + ++ + P +L GA+DP G + RLAE R Sbjct: 209 GFDASVALWIDIFHMIRRGANNRNFASIPKHLPFHLIGGAEDPATAGGAAIQRLAERMRR 268 Query: 270 QGISDVEYRLYPEGRHEMLNEVNRDEVTADVLDWLVRHLPAES 312 G V + + P RHE LNE+NRD VT L+WL+ LP+ + Sbjct: 269 MGFDRVTHNILPATRHESLNEINRDAVTQKFLNWLMEALPSRA 311 >AGc3857 [I] COG2267 Lysophospholipase Length = 289 Score = 118 bits (295), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 0/299 (0%), Positives = 0/299 (0%), Gaps = 6/299 (2%) Query: 7 KMTDPLGVDIHVYEWLPEPEAPVRGIVQIAHGMCEXXXXXXXXXXXXXXXXXXXXXNDHR 66 + + + G + Sbjct: 19 GYPIKHSRSP-IIHSYWLKKFGIAGSYTAVEVSPDDFPKFIATLKEGKPGAAVGGNATIP 77 Query: 67 GHGQTAGRVELLGDTG-ENGFYWMRRNLLQLAGIARSRHEGLPIFLLAHSMGSFLAQKLM 125 + E G + G L G Q+ + Sbjct: 78 HKEAAYRLADHPDALAEELGAANTIWMEEGKLHATNTDGYGFVSNLDERHPGWDKTQRAV 137 Query: 126 CEEGRDIYAGFILSGTN-GPRTMLRLGESLAGLQFRLQGKRHRSVLLNGIVFGPYNRSFT 184 + ++ + + L + V + Sbjct: 138 VFG-AGGASRAVIQSLRDRDVAEIHVVNRTVERARELADRFGPRVFSHPQAALQEVMHGA 196 Query: 185 PVRTAFDWLSSDTQEVDRFIADPFCGAICTTXXXXXXXXXXXXIHSRETLVTLCRDKPVY 244 + L + E T I + + + Sbjct: 197 GLFVNTTSLGMNGTEAPHLDFSHLAANAVVTDIVYVPLKTP--ILNMAQEQGIATVDGLG 254 Query: 245 LFAGAKDPVGMNGQGVLRLAEIYREQGISDVEYRLYPEGRHEMLNEVNRDEVTADVLDW 303 Sbjct: 255 313 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.141 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,169,498 Number of extensions: 902723 Number of successful extensions: 2320 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 2302 Number of HSP's successfully gapped: 7 Length of query: 320 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 214 Effective length of database: 26,153,027 Effective search space: 5596747778 Effective search space used: 5596747778 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)