BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0037.p (591 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2900 [L] COG0514 Superfamily II DNA helicase 562 e-160 BS_yocI [L] COG0514 Superfamily II DNA helicase 528 e-149 SA0676 [L] COG0514 Superfamily II DNA helicase 438 e-123 alr0205 [L] COG0514 Superfamily II DNA helicase 408 e-113 ZrecQ [L] COG0514 Superfamily II DNA helicase 391 e-108 >lin2900 [L] COG0514 Superfamily II DNA helicase Length = 590 Score = 562 bits (1449), Expect = e-160, Method: Compositional matrix adjust. Identities = 263/484 (54%), Positives = 358/484 (73%), Gaps = 2/484 (0%) Query: 11 MEQAQAELQKYYGYPDFREGQKKIVMNLLEGRDTLGIMPTGGGKSICYQVPALLLPGLTL 70 +EQA+A LQ+ +GY DFR+GQ ++ L G DTL IMPTGGGKS+CYQ+PALL GLT+ Sbjct: 2 IEQARAILQQNFGYQDFRDGQIDVISKLSAGEDTLAIMPTGGGKSLCYQIPALLFDGLTI 61 Query: 71 VISPLISLMKDQVDALTTAGIPAAYINSTLSGKEVNERIRAARRGELKLLYVAPERLELD 130 V+SPLISLMKDQVDAL + GI A +INSTL+ +E++ R+ AA GELK+LY+APER+E Sbjct: 62 VVSPLISLMKDQVDALVSDGIAATFINSTLTNREIDIRLDAAFSGELKMLYIAPERIETP 121 Query: 131 WFRLEMAGLSISCVAVDEAHCVSQWGHDFRTSYLAVSPFVDELPERPILAAFTATATPEV 190 F+ + + IS A+DEAHC+SQWGHDFR SYL++ +D++ RP++ A TATAT V Sbjct: 122 GFQRLIEQVPISLFAIDEAHCISQWGHDFRPSYLSLCDSLDKMTRRPLVIALTATATQAV 181 Query: 191 MEDMVRLLRLRQPGVFMTGLGRDNLAMSVLRGENKREFVLDYTARHSHQPGIVYAATRKE 250 +D+ RLL+++ V TG RDNLA V++G++K ++++DY ++S + GI+YA+TRKE Sbjct: 182 SDDICRLLKIKPNSVIKTGFSRDNLAFQVVKGQDKDKYLIDYLTKNSTESGIIYASTRKE 241 Query: 251 VDDLYQRLQASGIAAGRYHAGMSDQERAESQEGFLYDDIRVMVATNAFGMGIDKSNVRYV 310 V+ ++ L G+ +G YH GM+D R + QE FLYDDIRV+VATNAFGMGI+KSNVR+V Sbjct: 242 VERIHSFLLKKGVESGMYHGGMTDIARKDWQEKFLYDDIRVIVATNAFGMGINKSNVRFV 301 Query: 311 IHYNMPKNMEAYVQEAGRAGRDGEPSQCILLFSAQDIMTQKFLIEQNPQDADRKANEYRK 370 IHYN+P+N+EAY QEAGRAGRDG PS CILLFS QD Q+FLIEQ+ D +RK NE+ K Sbjct: 302 IHYNIPRNIEAYYQEAGRAGRDGVPSDCILLFSPQDSRIQQFLIEQSEMDDERKQNEFAK 361 Query: 371 LQQMIDYCYTTRCLRSAQLDYFGEEHGDQPCGICSSCTDERELVDMTVDAQKIFSCIHRM 430 L+QM Y YT CL+ + YFG++ ++ CG CS+C D RE D+T+ AQ++FSCI RM Sbjct: 362 LRQMTGYGYTEICLQKYIVQYFGDD--EENCGKCSNCLDTRETTDITILAQQVFSCIKRM 419 Query: 431 RERFGVALVSSVLKGSRNQKVLQYGFENLPTHGAMSSRTEKEITESINVLISEGYLALSE 490 ERFG L++ VL GS +QKV + FE L T+G M ++K++ + I+ L +E YL ++ Sbjct: 420 GERFGKVLIAKVLTGSADQKVKDWRFEELSTYGLMKDASQKDVLQLIDYLTAEKYLQPTD 479 Query: 491 GQYP 494 Q+P Sbjct: 480 SQFP 483 >BS_yocI [L] COG0514 Superfamily II DNA helicase Length = 591 Score = 528 bits (1359), Expect = e-149, Method: Compositional matrix adjust. Identities = 250/488 (51%), Positives = 349/488 (71%), Gaps = 5/488 (1%) Query: 11 MEQAQAELQKYYGYPDFREGQKKIVMNLLEGR-DTLGIMPTGGGKSICYQVPALLLPGLT 69 + +AQ+ L Y+GY FR GQ + + + E R +T IMPTGGGKSICYQ+PAL+ G T Sbjct: 2 LHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGTT 61 Query: 70 LVISPLISLMKDQVDALTTAGIPAAYINSTLSGKEVNERIRAARRGELKLLYVAPERL-E 128 +VISPLISLMKDQVDAL AGI AAYINST S +E+ ER+ + G KL Y+ PERL Sbjct: 62 IVISPLISLMKDQVDALEEAGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLTS 121 Query: 129 LDWFRLEMAGLSISCVAVDEAHCVSQWGHDFRTSYLAVSPFVDELPERPILAAFTATATP 188 +++ R+ + G+ + VA+DEAHC+SQWGHDFR SY + EL ++P++ A TATATP Sbjct: 122 IEFIRI-LQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEILFRELHDKPVIMALTATATP 180 Query: 189 EVMEDMVRLLRLRQPGVFMTGLGRDNLAMSVLRGENKREFVLDYTARHSHQPGIVYAATR 248 EV +D+ + L +++ TG R+NL V++GENK F+ +Y + H+ GIVY ATR Sbjct: 181 EVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR 240 Query: 249 KEVDDLYQRLQASGIAAGRYHAGMSDQERAESQEGFLYDDIRVMVATNAFGMGIDKSNVR 308 KE D +Y+RL+ + + AGRYH G++D R E QE FL D+++VMVAT+AFGMGIDKSN+R Sbjct: 241 KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIR 300 Query: 309 YVIHYNMPKNMEAYVQEAGRAGRDGEPSQCILLFSAQDIMTQKFLIEQNPQDADRKANEY 368 +V+H +PK+ME+Y QEAGRAGRDG S+C+LLFS QDIM Q+FLIEQ+ + +++ + Sbjct: 301 FVLHAQIPKDMESYYQEAGRAGRDGLASECVLLFSPQDIMVQRFLIEQSEHE-EKQKQDL 359 Query: 369 RKLQQMIDYCYTTRCLRSAQLDYFGEEHGDQPCGICSSCTDERELVDMTVDAQKIFSCIH 428 +KL+QM+DYC+T CL+ L YFGE+ D CG C +CTD R D+T +AQ + SCI Sbjct: 360 KKLRQMVDYCHTEDCLQRFILMYFGEKEPD-ACGQCGNCTDTRAAHDVTREAQMVLSCII 418 Query: 429 RMRERFGVALVSSVLKGSRNQKVLQYGFENLPTHGAMSSRTEKEITESINVLISEGYLAL 488 RM+ERFG +V+ VL GS+N+KVL+ GF +L T+G + ++ EI++ I LIS+ ++ + Sbjct: 419 RMKERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRM 478 Query: 489 SEGQYPVV 496 S+G +P + Sbjct: 479 SDGTFPTL 486 >SA0676 [L] COG0514 Superfamily II DNA helicase Length = 593 Score = 438 bits (1127), Expect = e-123, Method: Compositional matrix adjust. Identities = 218/472 (46%), Positives = 302/472 (63%) Query: 15 QAELQKYYGYPDFREGQKKIVMNLLEGRDTLGIMPTGGGKSICYQVPALLLPGLTLVISP 74 Q L Y+GY FR GQ++I+ +L+ R+ LG++PTGGGKSICYQVP LLL G T+VISP Sbjct: 3 QQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSICYQVPGLLLGGTTIVISP 62 Query: 75 LISLMKDQVDALTTAGIPAAYINSTLSGKEVNERIRAARRGELKLLYVAPERLELDWFRL 134 LISLMKDQVD L GI AA++NS+L+ KE +A GE++ LYVAPER E +F Sbjct: 63 LISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSNGEIQFLYVAPERFENRYFLN 122 Query: 135 EMAGLSISCVAVDEAHCVSQWGHDFRTSYLAVSPFVDELPERPILAAFTATATPEVMEDM 194 + + I VA DEAHC+S+WGHDFR SY V V LP+ + A TATAT EV +D+ Sbjct: 123 MLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFTLPQDFTIIALTATATVEVQQDI 182 Query: 195 VRLLRLRQPGVFMTGLGRDNLAMSVLRGENKREFVLDYTARHSHQPGIVYAATRKEVDDL 254 L + Q T R NL V +++F+LDY H GI+Y +TRK+V++L Sbjct: 183 REKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFILDYIKTHDEDAGIIYCSTRKQVEEL 242 Query: 255 YQRLQASGIAAGRYHAGMSDQERAESQEGFLYDDIRVMVATNAFGMGIDKSNVRYVIHYN 314 + L++ I + YHAG+S++ER E+Q FL+D ++V+VATNAFGMGIDKSNVR+VIHYN Sbjct: 243 QEALESQKIESVIYHAGLSNKEREEAQNDFLFDRVKVVVATNAFGMGIDKSNVRFVIHYN 302 Query: 315 MPKNMEAYVQEAGRAGRDGEPSQCILLFSAQDIMTQKFLIEQNPQDADRKANEYRKLQQM 374 MP ++E+Y QEAGRAGRDG S+CILLFS +DI ++ I + D D K KL +M Sbjct: 303 MPGDLESYYQEAGRAGRDGLKSECILLFSERDINLHEYFITVSQADDDYKDKMGEKLTKM 362 Query: 375 IDYCYTTRCLRSAQLDYFGEEHGDQPCGICSSCTDERELVDMTVDAQKIFSCIHRMRERF 434 I Y T +CL + + YF + C CS+C + + +MT +A+ I SCI RM+++ Sbjct: 363 IQYTKTKKCLEATIVHYFEPNEKLEECEQCSNCVQQDKSYNMTQEAKMIISCIARMKQQE 422 Query: 435 GVALVSSVLKGSRNQKVLQYGFENLPTHGAMSSRTEKEITESINVLISEGYL 486 +++ VL+G + G++ + THG M T E++ I+ L +G+L Sbjct: 423 SYSVIIQVLRGESTDYIKYKGYDQISTHGLMKGYTTSELSHLIDELRFKGFL 474 >alr0205 [L] COG0514 Superfamily II DNA helicase Length = 359 Score = 408 bits (1049), Expect = e-113, Method: Compositional matrix adjust. Identities = 0/496 (0%), Positives = 0/496 (0%), Gaps = 12/496 (2%) Query: 6 MQAPTMEQAQAELQKYYGYPDFREGQKKIVMNLLEGRDTLGIMPTGGGKSICYQVPALLL 65 A + + RE M + + G +++ + Sbjct: 2 QAATVVINR---RALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVA 58 Query: 66 PGLTLVISPLISLMKDQVDALTTAGIPAAYINSTLSGKEVNERIRAARRGELKLLYVAPE 125 + + K + S E L Sbjct: 59 RLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVT 118 Query: 126 RLE----LDWFRLEMAGLSISCVAVDEAHCVSQWGHDFRTSYLAVSPFVDELPERPILAA 181 + + + + + + E+ + Sbjct: 119 VWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAI- 177 Query: 182 FTATATPEVMEDMVRLLRLRQPGVFMTGLGRDNLAMSVLRGENKREFV-LDYTARHSHQP 240 F + + + + + Sbjct: 178 FNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILY-GVSPLEDRSTGADFGCQPVMS 236 Query: 241 GIVYAATRKEVDDLYQRLQASGIAAGRYHAGMSDQERAESQEGFLYDDIRVMVATNAFGM 300 +E + R ++ Sbjct: 237 LTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVP 296 Query: 301 GIDKSNVRYVIHYNMPKNMEAYVQEAGRAGRDGEPSQCILLFSAQDIMTQKFLIEQNPQD 360 + + + + P+ + G + + L + Sbjct: 297 IVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERIAEMTKVSAYELITRLTSRVA-M 355 Query: 361 ADRKANEYRKLQQMIDYCYTTRCLRSAQLDYFGEEHGDQPCGICSSCTDERELVDMTVDA 420 Sbjct: 356 - 414 Query: 421 QKIFSCIHRMRERFGVALVSSVLKGSRNQKVLQYGFENLPTHGAMSSRTEKEITESINVL 480 Sbjct: 415 474 Query: 481 ISEGYLALSEGQYPVV 496 Sbjct: 475 490 >ZrecQ [L] COG0514 Superfamily II DNA helicase Length = 610 Score = 391 bits (1004), Expect = e-108, Method: Compositional matrix adjust. Identities = 201/480 (41%), Positives = 293/480 (61%), Gaps = 3/480 (0%) Query: 14 AQAELQKYYGYPDFREGQKKIVMNLLEGRDTLGIMPTGGGKSICYQVPALLLPGLTLVIS 73 A+ LQ+ +GY FR GQ++I+ +L GRD L +MPTGGGKS+CYQ+PALLL GLT+V+S Sbjct: 15 AKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVS 74 Query: 74 PLISLMKDQVDALTTAGIPAAYINSTLSGKEVNERIRAARRGELKLLYVAPERLELDWFR 133 PLISLMKDQVD L G+ AA +NST + ++ E + R G+++LLY+APERL LD F Sbjct: 75 PLISLMKDQVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFL 134 Query: 134 LEMAGLSISCVAVDEAHCVSQWGHDFRTSYLAVSPFVDELPERPILAAFTATATPEVMED 193 +A + +AVDEAHC+SQWGHDFR Y A+ P P + A TATA +D Sbjct: 135 EHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPTLPFM-ALTATADDTTRQD 193 Query: 194 MVRLLRLRQPGVFMTGLGRDNLAMSVLRGENKREFVLDYTARHSHQPGIVYAATRKEVDD 253 +VRLL L P + ++ R N+ ++ + ++ Y + GI+Y +R +V+D Sbjct: 194 IVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVED 253 Query: 254 LYQRLQASGIAAGRYHAGMSDQERAESQEGFLYDDIRVMVATNAFGMGIDKSNVRYVIHY 313 RLQ+ GI+A YHAG+ + RA+ QE F DD++++VAT AFGMGI+K NVR+V+H+ Sbjct: 254 TAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHF 313 Query: 314 NMPKNMEAYVQEAGRAGRDGEPSQCILLFSAQDIMTQKFLIEQNPQDADRKANEYRKLQQ 373 ++P+N+E+Y QE GRAGRDG P++ +L + D+ + +E+ PQ + E KL Sbjct: 314 DIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQ-GQLQDIERHKLNA 372 Query: 374 MIDYCYTTRCLRSAQLDYFGEEHGDQPCGICSSCTDERELVDMTVDAQKIFSCIHRMRER 433 M + C R L+YFGE +PCG C C D + D + DAQ S I R+ +R Sbjct: 373 MGAFAEAQTCRRLVLLNYFGEGR-QEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQR 431 Query: 434 FGVALVSSVLKGSRNQKVLQYGFENLPTHGAMSSRTEKEITESINVLISEGYLALSEGQY 493 FG+ V V++G+ NQ++ YG + L +G ++ + I LI G + + Q+ Sbjct: 432 FGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQH 491 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,973,930 Number of extensions: 1484928 Number of successful extensions: 5995 Number of sequences better than 1.0e-05: 144 Number of HSP's gapped: 5864 Number of HSP's successfully gapped: 144 Length of query: 591 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 479 Effective length of database: 25,461,023 Effective search space: 12195830017 Effective search space used: 12195830017 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)