BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0017.p (428 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjoB [O] COG0465 ATP-dependent Zn proteases 507 e-143 YDL126c [O] COG0464 ATPases of the AAA+ class 88 3e-17 AF1297 [O] COG0464 ATPases of the AAA+ class 87 4e-17 MA1813 [O] COG0464 ATPases of the AAA+ class 86 1e-16 PH1840 [O] COG0464 ATPases of the AAA+ class 86 1e-16 >BS_yjoB [O] COG0465 ATP-dependent Zn proteases Length = 423 Score = 507 bits (1305), Expect = e-143, Method: Compositional matrix adjust. Identities = 251/419 (59%), Positives = 311/419 (74%), Gaps = 3/419 (0%) Query: 11 IYKYDENATG-MIKGYDAYARLVDGILEALHTRYGVRYELYASDDPNSEYWKLLENDVQS 69 IY+Y+E GY + L+ I E L+ +YGV YELYASDDPN+EYW+LL DV+S Sbjct: 7 IYQYEEKENERAAAGYGTFGYLITRIEETLYDQYGVFYELYASDDPNTEYWELLVEDVRS 66 Query: 70 GHPGVEHVARIFDRLEDRTFVYDDDKEQPEYSIHLSIRNNVLAYPEMGVALARVPVFQEN 129 G EHVA IF++LE +TF YD+D+++P+Y++H SIRN+V AYPE GVA AR+P FQ+ Sbjct: 67 GSLEPEHVAYIFEKLEKKTFAYDEDEKEPDYTVHKSIRNSVYAYPEKGVAFARIPYFQDG 126 Query: 130 GINFQDYVFAASDGQLQGFLGNVRKRQREQNINKVTVFTDRRNGIFREDEPITRSVGREA 189 I D +FA +D +++ FL VR R E++ KVTVFTD G RE E I R V R Sbjct: 127 SIMSFDCLFAVNDEKMRAFLEGVRPRLWEKSKRKVTVFTDGDGGTSREQEAIVREVQRSQ 186 Query: 190 VVLEAGIKKEIYRSLDQFFDSDRSFYVTYDIPYKRGILLYGHPGNGKTTLVKSIAGSVPG 249 V++ +KKEIYRS+DQFF SD+SFY TYDIPYKRGILLYG PGNGKTTLVKSIAGS+ Sbjct: 187 VIMNPLLKKEIYRSIDQFFHSDKSFYQTYDIPYKRGILLYGPPGNGKTTLVKSIAGSIDA 246 Query: 250 PVVYWQITEYTSSESVNEVFEAAARLAPMVLVIEDIDSMPQEVRSFFLNTLDGATSKEGI 309 PV YWQITE+TSSE++ EVF+AA RLAP VLVIEDIDSMP++VRSFFLNTLDGATSKEG+ Sbjct: 247 PVAYWQITEFTSSETIEEVFQAARRLAPAVLVIEDIDSMPEDVRSFFLNTLDGATSKEGL 306 Query: 310 FLIGTTNYPEKIDPGLMNRAGRFDRAYEIKMPDEELRLEYLKLRXXXXXXXXXXXXXXXR 369 FLIGTTNYPE+IDPGLMNRAGRFDRAYEI +PDEELRLEY+K+R + Sbjct: 307 FLIGTTNYPEEIDPGLMNRAGRFDRAYEIGLPDEELRLEYMKMRGFGIFLSEGEIKNAAK 366 Query: 370 LTADFSLTQLGELYVSAALEWHENGRADVEALVRGMRGELDKGRKREWLKDASSTIGFY 428 LT FS QLGELYVS+AL+WH+ G +E +V+ M GE K ++ W++ + +GF+ Sbjct: 367 LTEGFSFAQLGELYVSSALQWHQEGNHHIETMVKDMTGEQRKSQRGSWME--RNKVGFH 423 >YDL126c [O] COG0464 ATPases of the AAA+ class Length = 835 Score = 87.8 bits (216), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 53/160 (33%), Positives = 84/160 (52%), Gaps = 29/160 (18%) Query: 215 YVTYDIPYKRGILLYGHPGNGKTTLVKSIAG-------SVPGP--VVYWQITEYTSSES- 264 Y + + +G+L YG PG GKT L K++A SV GP + W Y SES Sbjct: 513 YTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSMW----YGESESN 568 Query: 265 VNEVFEAAARLAPMVLVIEDIDSMPQ--------------EVRSFFLNTLDGATSKEGIF 310 + ++F+ A AP V+ ++++DS+ + V + L +DG +K+ +F Sbjct: 569 IRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAKKNVF 628 Query: 311 LIGTTNYPEKIDPGLMNRAGRFDRAYEIKMPDEELRLEYL 350 +IG TN P++IDP ++ R GR D+ + +PDE RL L Sbjct: 629 VIGATNRPDQIDPAIL-RPGRLDQLIYVPLPDENARLSIL 667 Score = 85.1 bits (209), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 51/157 (32%), Positives = 81/157 (51%), Gaps = 20/157 (12%) Query: 214 FYVTYDIPYKRGILLYGHPGNGKTTLVKSIAGS-------VPGPVVYWQITEYTSSESVN 266 + I RG+L+YG PG GKT + +++A + GP V ++ + S ++ Sbjct: 239 LFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFFFLINGPEVMSKMAGESES-NLR 297 Query: 267 EVFEAAARLAPMVLVIEDIDSMP-----------QEVRSFFLNTLDGATSKEGIFLIGTT 315 + FE A + AP ++ I++IDS+ + V S L +DG ++ + +I T Sbjct: 298 KAFEEAEKNAPAIIFIDEIDSIAPKRDKTNGEVERRVVSQLLTLMDGMKARSNVVVIAAT 357 Query: 316 NYPEKIDPGLMNRAGRFDRAYEIKMPDEELRLEYLKL 352 N P IDP L R GRFDR +I +PD RLE L++ Sbjct: 358 NRPNSIDPAL-RRFGRFDREVDIGIPDATGRLEVLRI 393 >AF1297 [O] COG0464 ATPases of the AAA+ class Length = 733 Score = 87.4 bits (215), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 69/217 (31%), Positives = 103/217 (47%), Gaps = 34/217 (15%) Query: 195 GIKKEI--YRSLDQFFDSDRSFYVTYDIPYKRGILLYGHPGNGKTTLVKSIAGSVPG--- 249 G+K+E+ R + + + I +G+LLYG PG GKT + K++A V Sbjct: 186 GLKRELRLVREMIELPLKHPELFQRLGIEPPKGVLLYGPPGTGKTLIAKAVANEVDAHFI 245 Query: 250 PVVYWQITE--YTSSES-VNEVFEAAARLAPMVLVIEDIDSMP-----------QEVRSF 295 P+ +I Y SE + E+FE A AP ++ I++IDS+ + V + Sbjct: 246 PISGPEIMSKYYGESEQRLREIFEEAKENAPSIIFIDEIDSIAPKREEVTGEVERRVVAQ 305 Query: 296 FLNTLDGATSKEGIFLIGTTNYPEKIDPGLMNRAGRFDRAYEIKMPDEELRLEYLKLRXX 355 L +DG ++ + +I TN P+ IDP L R GRFDR EI +PD+E R E L++ Sbjct: 306 LLALMDGLEARGDVIVIAATNRPDAIDPAL-RRPGRFDREIEIGVPDKEGRKEILEIHTR 364 Query: 356 XXXXXXXXXXXXXRLTADFSLTQLGEL---YVSAALE 389 L D L +L EL +V A LE Sbjct: 365 KMP-----------LAEDVDLEELAELTNGFVGADLE 390 Score = 81.3 bits (199), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 70/231 (30%), Positives = 106/231 (45%), Gaps = 44/231 (19%) Query: 219 DIPYKRGILLYGHPGNGKTTLVKSIAG-------SVPGPVVYWQITEYTSSESVNEVFEA 271 +I RGILL+G PG GKT L K++A SV GP + + S + V E+F Sbjct: 485 NIKPPRGILLFGPPGTGKTLLAKAVANESNANFISVKGPELLSKWV-GESEKHVREMFRK 543 Query: 272 AARLAPMVLVIEDIDSMP------------QEVRSFFLNTLDGATSKEGIFLIGTTNYPE 319 A ++AP V+ ++IDS+ + V S L LDG + + +I TN P+ Sbjct: 544 ARQVAPCVIFFDEIDSLAPRRGGIGDSHVTERVVSQLLTELDGLEELKDVVVIAATNRPD 603 Query: 320 KIDPGLMNRAGRFDRAYEIKMPDEELRLEYLKLRXXXXXXXXXXXXXXXRLTADFSLTQL 379 IDP L+ R GR +R I PD++ R+E K+ L D ++ +L Sbjct: 604 MIDPALL-RPGRLERHIYIPPPDKKARVEIFKIHLRGKP-----------LADDVNIEEL 651 Query: 380 GELYVSAALEWHENGRADVEALVR-----GMRGELDKGRKREWLKDASSTI 425 E E + AD+EA+ R +R + G RE K+A+ + Sbjct: 652 AE-----KTEGYSG--ADIEAVCREAGMLAIRELIKPGMTREEAKEAAKKL 695 >MA1813 [O] COG0464 ATPases of the AAA+ class Length = 764 Score = 86.3 bits (212), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 59/195 (30%), Positives = 97/195 (49%), Gaps = 35/195 (17%) Query: 190 VVLEAGIKKEIYRSLDQFFDSDRSFYVTYDIPYK-------------RGILLYGHPGNGK 236 ++ E I +Y L D+ ++P K +G+LL G PG GK Sbjct: 204 IISEQNIPTVMYEDLGGLKDAISKVREMIELPLKHPELFDRLGIDAPKGVLLQGPPGTGK 263 Query: 237 TTLVKSIAG-------SVPGPVVYWQITEYTSSE-SVNEVFEAAARLAPMVLVIEDIDSM 288 T L K++A S+ GP + + Y SE ++ E+FE A + AP ++ +++IDS+ Sbjct: 264 TMLAKAVANESDAYFISINGPEIMSKY--YGESERAIREIFEDAEKNAPAIIFLDEIDSI 321 Query: 289 P-----------QEVRSFFLNTLDGATSKEGIFLIGTTNYPEKIDPGLMNRAGRFDRAYE 337 + V + L+ +DG +++ + +IG TN PE +D L R GRFDR E Sbjct: 322 APKRAEVTGEVERRVVAQLLSLMDGLKARKNVIVIGATNRPEALDIAL-RRPGRFDREIE 380 Query: 338 IKMPDEELRLEYLKL 352 +++PD E RLE ++ Sbjct: 381 LRVPDTEGRLEIFQI 395 Score = 76.3 bits (186), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 52/174 (29%), Positives = 88/174 (50%), Gaps = 20/174 (11%) Query: 198 KEIYRSLDQFFDSDRSFYVTYDIPYKRGILLYGHPGNGKTTLVKSIAGSVPGPVVYWQIT 257 KE+ + + ++ + Y + +G+LLYG PG GKT L K+IA + + + Sbjct: 498 KELLKEVVEWPLKNPESYRDIGVEAPKGVLLYGPPGTGKTLLAKAIAHESDANFITAKGS 557 Query: 258 E-----YTSSES-VNEVFEAAARLAPMVLVIEDIDSM----------PQ---EVRSFFLN 298 + Y SE + EVF A ++AP ++ ++++DS+ PQ + + L+ Sbjct: 558 DLLSKWYGESEKRIAEVFTRARQVAPSIIFLDELDSLAPIRGTSVGEPQVTARILNQLLS 617 Query: 299 TLDGATSKEGIFLIGTTNYPEKIDPGLMNRAGRFDRAYEIKMPDEELRLEYLKL 352 +DG + +IG TN P+ IDP L+ R GRFD + +PDE R E ++ Sbjct: 618 EMDGLEELRAVVVIGATNRPDIIDPALL-RPGRFDELILVPVPDEGARREIFRV 670 >PH1840 [O] COG0464 ATPases of the AAA+ class Length = 798 Score = 85.9 bits (211), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 56/175 (32%), Positives = 89/175 (50%), Gaps = 21/175 (12%) Query: 196 IKKEIYRSLDQFFDSDRSFYVTYDIPYKRGILLYGHPGNGKTTLVKSIAG-------SVP 248 +K+E+ +++ +F Y I +G+LLYG PG GKT L K++A +V Sbjct: 527 VKQELREAVEWPLKYPEAFR-AYGITPPKGVLLYGPPGTGKTLLAKAVATESEANFIAVR 585 Query: 249 GPVVYWQITEYTSSESVNEVFEAAARLAPMVLVIEDIDSMP-----------QEVRSFFL 297 GP V + S +++ E+F A + AP V+ I++ID++ + + L Sbjct: 586 GPEVLSKWV-GESEKNIREIFRKARQAAPTVIFIDEIDAIAPRRGTDVNRVTDRLINQLL 644 Query: 298 NTLDGATSKEGIFLIGTTNYPEKIDPGLMNRAGRFDRAYEIKMPDEELRLEYLKL 352 +DG G+ +I TN P+ +DP L+ R GRFDR + PDEE R E K+ Sbjct: 645 TEMDGIQENTGVVVIAATNRPDILDPALL-RPGRFDRLILVPAPDEEARFEIFKV 698 Score = 82.8 bits (203), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 51/149 (34%), Positives = 83/149 (55%), Gaps = 24/149 (16%) Query: 224 RGILLYGHPGNGKTTLVKSIAG-------SVPGPVVYWQITEY--TSSESVNEVFEAAAR 274 +G+LLYG PG GKT L K++A ++ GP + +++Y S E + EVF+ A Sbjct: 220 KGVLLYGPPGTGKTLLAKAVANEANAYFIAINGPEI---MSKYYGESEERLREVFKEAEE 276 Query: 275 LAPMVLVIEDIDSMP-----------QEVRSFFLNTLDGATSKEGIFLIGTTNYPEKIDP 323 AP ++ I++ID++ + V + L +DG + + +IG TN P+ +DP Sbjct: 277 NAPSIIFIDEIDAIAPKRSEVTGEVEKRVVAQLLALMDGLKGRGKVIVIGATNRPDALDP 336 Query: 324 GLMNRAGRFDRAYEIKMPDEELRLEYLKL 352 L R GRFDR E+ +PD++ R E L++ Sbjct: 337 AL-RRPGRFDREIEVGVPDKQGRKEILQI 364 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,146,867 Number of extensions: 1372054 Number of successful extensions: 17438 Number of sequences better than 1.0e-05: 113 Number of HSP's gapped: 17153 Number of HSP's successfully gapped: 139 Length of query: 428 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 319 Effective length of database: 25,807,025 Effective search space: 8232440975 Effective search space used: 8232440975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)