BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0016.p (484 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA0266 [NU] COG4969 Tfp pilus assembly protein, major pilin PilA 210 5e-54 MJ0682_3 [S] COG1690 Uncharacterized conserved protein 174 3e-43 PH1602_3 [S] COG1690 Uncharacterized conserved protein 173 5e-43 PAB0383_3 [S] COG1690 Uncharacterized conserved protein 171 2e-42 MK1682_3 [S] COG1690 Uncharacterized conserved protein 154 2e-37 >NMA0266 [NU] COG4969 Tfp pilus assembly protein, major pilin PilA Length = 500 Score = 210 bits (534), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 152/474 (32%), Positives = 223/474 (47%), Gaps = 27/474 (5%) Query: 20 LALPGGDLKVYASEQLFATLDYKVLEMANNNLQIPNIE-YMSYTPDVHVGVGTCIGTTAV 78 + +PG +++ SE L ++ ++ N +P I+ Y PD H+G G IG A Sbjct: 43 MRVPG---RLFVSESLLEGIEPGTIDQIANVATLPGIQKYSMAMPDAHLGYGFAIGGVAA 99 Query: 79 WDAADGYVSPSIVGSDIGCGMRVHLTTLHXXXXXXXXXXXXXXXAIEKYLPMEQQQRGTY 138 +D +G +SP VG DI CG+R+ T L + + R Sbjct: 100 FDVEEGIISPGGVGFDINCGVRLIRTNLQKEDVVPEIKKLTDELFTNVPAGVGSKSRFRA 159 Query: 139 SDIRLENIVRKGLHGLPNKYVPDSYTPKKSSALSHVEISKL--GFDEEILNELPDMAWHR 196 SD L++ +G K+ D+ + + H E + G D ++ A +R Sbjct: 160 SDRELDSAFLEGA-----KWAVDAGYGVDAD-VEHCEGNGYLEGADTSYVS---TKARNR 210 Query: 197 GHRQLGTLGGGNHFVXXXXXXXXXXXXXXXXXWGLRDGQIAVMIHSGSRAWGGMVNQFCI 256 G QLGTLG GNHF+ +GL +GQ+ VM+H GSR G + + Sbjct: 211 GKPQLGTLGSGNHFLEVQYVDEIYDREVASA-FGLEEGQVTVMVHCGSRGAGHQICTDHL 269 Query: 257 PAFAKVMGQLGLGSADPRLIYAPLAHPQARRYVNLMYSALNYAVVNRHLIAYAVREGFRD 316 ++ + + G+ D +L AP +A+ Y M A NYA NR +I + RE F Sbjct: 270 KELSQAVKKYGIEIPDKQLACAPAQSREAQNYFKAMLCAANYAWANRQMITHWTRESFEK 329 Query: 317 VFGPKCE---LRTLYDLMHNYA-WQEQTAAGTK---FVHRKGATRALPAGHPDNPRPYAA 369 VFG + + LYD+ HN A +E G K +VHRKGATRA PAGHP+ P Y Sbjct: 330 VFGRDADEMGMSLLYDVAHNVAKLEEHNIEGRKKEVYVHRKGATRAFPAGHPEVPAAYRD 389 Query: 370 TGHPALIPGSMGTASYIMVG-QPGGEENYYSICHGAGRIRSRSATKRLVSVNDFSKSLRV 428 G P LIPGSMGT S+I+ G + + ++ S CHGAGR+ SR+ K+ ++L Sbjct: 390 VGQPVLIPGSMGTPSFILCGAKDAMDVSFGSACHGAGRVMSRAHAKKEFRGQSVKENLEA 449 Query: 429 GREDEIVVNQHSLESIIDESPQAYKNVDEIIESVTGAGLAAVVAKCKPLAAIKG 482 I V I +E+P YK+ E++ V G+A VA+ PL KG Sbjct: 450 ---HGITVRATHPSVIAEEAPGVYKSSSEVVNVVHELGIARKVARVIPLGVAKG 500 >MJ0682_3 [S] COG1690 Uncharacterized conserved protein Length = 384 Score = 174 bits (440), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 107/302 (35%), Positives = 154/302 (50%), Gaps = 12/302 (3%) Query: 189 LPDMAWHRGHRQLGTLGGGNHFVXXXXXXXXXXXXXXXXXWGLRDGQIAVMIHSGSRAWG 248 + D A RG QLG+LG GNHF+ +G+ + Q+ V++H+GSR G Sbjct: 87 VSDKAKERGRVQLGSLGSGNHFLEVQYVEKVFDEEAAEI-YGIEENQVVVLVHTGSRGLG 145 Query: 249 GMVNQFCIPAFAKVMGQLGLGSADPRLIYAPLAHPQARRYVNLMYSALNYAVVNRHLIAY 308 + + K G+ D +L AP + + Y M NYA NR +I + Sbjct: 146 HQICTDYLRIMEKAAKNYGIKLPDRQLACAPFESEEGQSYFKAMCCGANYAWANRQMITH 205 Query: 309 AVREGFRDVF---GPKCELRTLYDLMHNYAWQEQ-TAAGTK---FVHRKGATRALPAGHP 361 VRE F +VF E+ +YD+ HN A +E+ G K VHRKGATRA P H Sbjct: 206 WVRESFEEVFKIHAEDLEMNIVYDVAHNIAKKEEHIIDGRKVKVIVHRKGATRAFPPKHE 265 Query: 362 DNPRPYAATGHPALIPGSMGTASYIMVG-QPGGEENYYSICHGAGRIRSRSATKRLVSVN 420 P+ Y + G P +IPG MGTASY+M G + +E + S HGAGR SR+ +L Sbjct: 266 AIPKEYWSVGQPVIIPGDMGTASYLMRGTEIAMKETFGSTAHGAGRKLSRAKALKLWKGK 325 Query: 421 DFSKSLRVGREDEIVVNQHSLESIIDESPQAYKNVDEIIESVTGAGLAAVVAKCKPLAAI 480 + + L E IV S + +E+P+AYK+VD + ++ AG++ VA+ +PL I Sbjct: 326 EIQRRL---AEMGIVAMSDSKAVMAEEAPEAYKSVDLVADTCHKAGISLKVARMRPLGVI 382 Query: 481 KG 482 KG Sbjct: 383 KG 384 >PH1602_3 [S] COG1690 Uncharacterized conserved protein Length = 385 Score = 173 bits (439), Expect = 5e-43, Method: Compositional matrix adjust. Identities = 116/312 (37%), Positives = 164/312 (52%), Gaps = 16/312 (5%) Query: 180 GFDEEILNELPDMAWHRGHRQLGTLGGGNHFVXXXXXXXXXXXXXXXXXWGLRDGQIAVM 239 G D E +++ A RG QLG+LG GNHF+ +GL +GQ+ VM Sbjct: 81 GADPEAVSQ---RAKQRGAPQLGSLGSGNHFLEVQVVDKIFDPEVAKA-YGLFEGQVVVM 136 Query: 240 IHSGSRAWGGMVNQFCIPAFAKVMGQLGLGSADPRLIYAPLAHPQARRYVNLMYSALNYA 299 +H+GSR G V + + + + + D L+ P + +RY + M +A N+A Sbjct: 137 VHTGSRGLGHQVASDYLRIMERAIRKYRIPWPDRELVSVPFQSEEGQRYFSAMKAAANFA 196 Query: 300 VVNRHLIAYAVREGFRDVFGPKCE----LRTLYDLMHNYAWQEQTAAGTK----FVHRKG 351 NR +I + VRE F++VF E + +YD+ HN E+ K VHRKG Sbjct: 197 WANRQMITHWVRESFQEVFKQDPEGDLGMDIVYDVAHNIGKVEEHEVDGKRVKVIVHRKG 256 Query: 352 ATRALPAGHPDNPRPYAATGHPALIPGSMGTASYIMVGQPGG-EENYYSICHGAGRIRSR 410 ATRA P GH PR Y G P LIPGSMGTASYI+ G G +E + S CHGAGR+ SR Sbjct: 257 ATRAFPPGHEAVPRLYRDVGQPVLIPGSMGTASYILAGTEGAMKETFGSTCHGAGRVLSR 316 Query: 411 SATKRLVSVNDFSKSLRVGREDEIVVNQHSLESIIDESPQAYKNVDEIIESVTGAGLAAV 470 A R + + L I V S+ + +E+P AYKNVD +++ V+ AG+A + Sbjct: 317 KAATRQYRGDRIRQEL---LNRGIYVRAASMRVVAEEAPGAYKNVDNVVKVVSEAGIAKL 373 Query: 471 VAKCKPLAAIKG 482 VA+ +P+ KG Sbjct: 374 VARMRPIGVAKG 385 >PAB0383_3 [S] COG1690 Uncharacterized conserved protein Length = 384 Score = 171 bits (434), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 112/298 (37%), Positives = 159/298 (53%), Gaps = 12/298 (4%) Query: 193 AWHRGHRQLGTLGGGNHFVXXXXXXXXXXXXXXXXXWGLRDGQIAVMIHSGSRAWGGMVN 252 A RG QLG+LG GNHF+ +GL +GQ+ VM+H+GSR G V Sbjct: 91 AKQRGAPQLGSLGSGNHFLEVQVVDKIFDPEVAKV-YGLFEGQVVVMVHTGSRGLGHQVA 149 Query: 253 QFCIPAFAKVMGQLGLGSADPRLIYAPLAHPQARRYVNLMYSALNYAVVNRHLIAYAVRE 312 + + + + + D L+ P + +RY + M +A N+A NR +I + VRE Sbjct: 150 SDYLRIMERAIRKYRIPWPDRELVSVPFQSEEGQRYFSAMKAAANFAWANRQMITHWVRE 209 Query: 313 GFRDVFGPKCE---LRTLYDLMHNYA-WQEQTAAGTK---FVHRKGATRALPAGHPDNPR 365 F++VF E + +YD+ HN +E G K VHRKGATRA P GH P+ Sbjct: 210 SFQEVFRQDPEDLGMSIVYDVAHNIGKVEEHEVDGKKVKVIVHRKGATRAFPPGHEAIPK 269 Query: 366 PYAATGHPALIPGSMGTASYIMVGQPGG-EENYYSICHGAGRIRSRSATKRLVSVNDFSK 424 Y G P LIPGSMGTASY++ G G +E + S CHGAGR+ SR A R + + Sbjct: 270 IYRDVGQPVLIPGSMGTASYVLAGTEGAMKETFGSTCHGAGRVLSRKAATRQYRGDRIRQ 329 Query: 425 SLRVGREDEIVVNQHSLESIIDESPQAYKNVDEIIESVTGAGLAAVVAKCKPLAAIKG 482 L I V S+ + +E+P AYKNVD +++ V+ AG+A +VA+ +P+ KG Sbjct: 330 EL---LNRGIYVRAASMRVVAEEAPGAYKNVDNVVKVVSEAGIAKLVARMRPIGVAKG 384 >MK1682_3 [S] COG1690 Uncharacterized conserved protein Length = 407 Score = 154 bits (390), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 111/312 (35%), Positives = 160/312 (51%), Gaps = 29/312 (9%) Query: 193 AWHRGHRQLGTLGGGNHFVXXXXXXXXXXXXXXXXXWGLRDGQIAVMIHSGSRAWGGMVN 252 A RG QLGTLG GNHF+ +GQ+ +M+H+GSR +G + Sbjct: 103 AIERGRPQLGTLGSGNHFLEVQVVDEIYDKEAAEKMGIREEGQVTIMVHTGSRGFG---H 159 Query: 253 QFCIP-------AFAKVMGQLGLGSADPRLIYAPLAHPQARRYVNLMYSALNYAVVNRHL 305 Q C + V + G+ D +L A + +A+RY N M +A NYA NR + Sbjct: 160 QVCSDHLRIMERSMRDVERRFGVRIPDRQLACAAMGTDEAKRYFNAMNAAANYAFANRQM 219 Query: 306 IAYAVREGFRDVFGPK------CELRTLYDLMHNYAWQEQTAAGTK----FVHRKGATRA 355 I++ RE F +VFG + + +YD+ HN A E+ + VHRKGATRA Sbjct: 220 ISHWTRESFVEVFGDEYGDADDMGIEVIYDIAHNMAKIEKHPVDGEERWLVVHRKGATRA 279 Query: 356 LPA---GHPDNPRPYAATGHPALIPGSMGTASYIMVG-QPGGEENYYSICHGAGRIRSRS 411 P P+ P LIPG MGT SYI++G + EE + S CHGAGR SR+ Sbjct: 280 FSEEALKKHGEPVPFEGLPQPVLIPGDMGTGSYILIGTEKAMEETWGSTCHGAGRTMSRA 339 Query: 412 ATKRLVSVNDFSKSL-RVGREDEIVVNQHSLESIIDESPQAYKNVDEIIESVTGAGLAAV 470 A KR D ++ L R G I+V S+ + +E+P AYK+VDE++ +V AG++ Sbjct: 340 AAKRKFWGEDVARELERQG----ILVKAASMPVVAEEAPPAYKDVDEVVRAVAEAGISDP 395 Query: 471 VAKCKPLAAIKG 482 V + +P+ +KG Sbjct: 396 VVRLRPIGVVKG 407 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,181,193 Number of extensions: 1493672 Number of successful extensions: 2642 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2584 Number of HSP's successfully gapped: 16 Length of query: 484 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 374 Effective length of database: 25,691,691 Effective search space: 9608692434 Effective search space used: 9608692434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)