BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0012.p (283 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv0439c [IQR] COG1028 Dehydrogenases with different specificitie... 144 1e-34 MT0455 [IQR] COG1028 Dehydrogenases with different specificities... 144 1e-34 VNG0479G [IQR] COG1028 Dehydrogenases with different specificiti... 142 7e-34 ML0315 [IQR] COG1028 Dehydrogenases with different specificities... 140 2e-33 Rv0068 [IQR] COG1028 Dehydrogenases with different specificities... 130 2e-30 >Rv0439c [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 311 Score = 144 bits (364), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 101/290 (34%), Positives = 139/290 (47%), Gaps = 16/290 (5%) Query: 5 GKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLCD 64 G+ V+VTG N G+G T A RGA V++A R+ +G A A ++ L D Sbjct: 21 GRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQQLD 80 Query: 65 LASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXXX 124 L S DSVRA A+ YP +DVLINNAGV+ +Q+T DG+EL FG NH Sbjct: 81 LCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALTGLV 140 Query: 125 XERLVAAEQGRIVVVASGAYKI-GKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQ 183 + ++ R+V V+S ++I +H +D R YN AY ++KLAN+LFT EL + Sbjct: 141 LDHMLPVPGSRVVTVSSQGHRIHAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYELQRR 200 Query: 184 L--RGTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSP-FFLTPEQGADTAIWLAAAP 240 L G A HPG T + N R +L P F +PE GA + A P Sbjct: 201 LGEAGKSTIAVAAHPGGSNTELTRNLPR-LIRPVATVLGPLLFQSPEMGALPTLRAATDP 259 Query: 241 ELRGVTGEYY---------YRRKIQELSPXXXXXXXXXQLWQWSLEHTGL 281 +G G+YY K+ + S +LW S E TG+ Sbjct: 260 TTQG--GQYYGPDGFGEQRGHPKVVQSSAQSHDKDLQRRLWTVSEELTGV 307 >MT0455 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 338 Score = 144 bits (364), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 93/250 (37%), Positives = 127/250 (50%), Gaps = 7/250 (2%) Query: 5 GKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLCD 64 G+ V+VTG N G+G T A RGA V++A R+ +G A A ++ L D Sbjct: 48 GRVVVVTGANTGIGYHTAAVFADRGAHVVLAVRNLEKGNAARARIMAARPGAHVTLQQLD 107 Query: 65 LASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXXX 124 L S DSVRA A+ YP +DVLINNAGV+ +Q+T DG+EL FG NH Sbjct: 108 LCSLDSVRAAADALRTAYPRIDVLINNAGVMWTPKQVTKDGFELQFGTNHLGHFALTGLV 167 Query: 125 XERLVAAEQGRIVVVASGAYKI-GKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQ 183 + ++ R+V V+S ++I +H +D R YN AY ++KLAN+LFT EL + Sbjct: 168 LDHMLPVPGSRVVTVSSQGHRIHAAIHFDDLQWERRYNRVAAYGQAKLANLLFTYELQRR 227 Query: 184 L--RGTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSP-FFLTPEQGADTAIWLAAAP 240 L G A HPG T + N R +L P F +PE GA + A P Sbjct: 228 LGEAGKSTIAVAAHPGGSNTELTRNLPR-LIRPVATVLGPLLFQSPEMGALPTLRAATDP 286 Query: 241 ELRGVTGEYY 250 +G G+YY Sbjct: 287 TTQG--GQYY 294 >VNG0479G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 316 Score = 142 bits (357), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 98/254 (38%), Positives = 139/254 (54%), Gaps = 12/254 (4%) Query: 5 GKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLCD 64 G+ V+VTG N+G+G T AR GA V+MACRS RGE+A + + ++ + D Sbjct: 17 GRRVVVTGANSGLGFEATRAFARAGAHVVMACRSTERGEDARDDIVAELPGASLTVHELD 76 Query: 65 LASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXXX 124 LA+ DSV AFA+ F A + L VL NNAGV+A+ R TADG+E FGVNH Sbjct: 77 LAALDSVAAFADWFTAEFDSLHVLANNAGVMAIPRSETADGFETQFGVNHLGHVALTAGL 136 Query: 125 XERL-VAAEQGRIVVVASGAYKIGKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQ 183 L + + R+V +SGA++ G++ ED Y +AY++SKLAN+LF EL + Sbjct: 137 LGVLRRTSGETRVVTQSSGAHRRGRIDFEDLQHEAEYGKWEAYSQSKLANLLFAYELDRR 196 Query: 184 LRGTQVTVNAV--HPGAVGTSIGVNRETGFGRSFLKLLSP------FFLTPEQGADTAIW 235 LR +V +V HPG T++ + G S L+LL+ + EQGA ++ Sbjct: 197 LRAASASVTSVACHPGYAATNLQLRGPQAAG-SRLRLLAMRAANALVGQSAEQGAWPLLY 255 Query: 236 LAAAPELRGVTGEY 249 A P + G GEY Sbjct: 256 AATNPSIDG--GEY 267 >ML0315 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 127 Score = 140 bits (353), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 0/254 (0%), Positives = 0/254 (0%), Gaps = 16/254 (6%) Query: 5 GKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLCD 64 + V +++A S K +S Sbjct: 14 KIFLFVKICEKKCRQKELKAFTLIEMLIVLAIISILILLFVPNLIKEKSQVQKTGEAAVV 73 Query: 65 LASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXXX 124 Sbjct: 74 133 Query: 125 XERLVAAEQGRIVVVASGAYKI-GKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQ 183 Sbjct: 134 193 Query: 184 LRGTQVTVN-AVHPGAVGTSIGVNRETGFGRSFLKLLSPFFLTPEQGADTAIWLAAAPEL 242 Sbjct: 194 -------- 245 Query: 243 RGVT------GEYY 250 Sbjct: 246 259 >Rv0068 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 303 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 87/249 (34%), Positives = 130/249 (52%), Gaps = 7/249 (2%) Query: 5 GKTVLVTGGNAGMGLATTIEMARRGAAVIMACRSRTRGEEALAEAKRQSGSDNIRLMLCD 64 G+T ++TG N G+G T +A GA V++A R+ +G++A A + + L D Sbjct: 14 GRTAVITGANTGLGFETAAALAAHGAHVVLAVRNLDKGKQAAARITEATPGAEVELQELD 73 Query: 65 LASFDSVRAFAEEFAAGYPVLDVLINNAGVVALKRQLTADGYELDFGVNHXXXXXXXXXX 124 L S SVRA A + + + +D+LINNAGV+ RQ TADG+E+ FG NH Sbjct: 74 LTSLASVRAAAAQLKSDHQRIDLLINNAGVMYTPRQTTADGFEMQFGTNHLGHFALTGLL 133 Query: 125 XERLVAAEQGRIVVVASGAYKI-GKLHLEDHTLSRGYNPAKAYARSKLANILFTRELAAQ 183 +RL+ R+V ++S ++I +H +D R Y AY ++KLAN+LFT EL + Sbjct: 134 IDRLLPVAGSRVVTISSVGHRIRAAIHFDDLQWERRYRRVAAYGQAKLANLLFTYELQRR 193 Query: 184 LR--GTQVTVNAVHPGAVGTSIGVNRETGFGRSFLKLLSPFFLTPEQGADTAIWLAAAPE 241 L GT + V A HPG T + N + +L+P E GA + A P Sbjct: 194 LAPGGTTIAV-ASHPGVSNTEVVRNMPRPL-VAVAAILAPLMQDAELGALPTLRAATDPA 251 Query: 242 LRGVTGEYY 250 +RG G+Y+ Sbjct: 252 VRG--GQYF 258 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,923,451 Number of extensions: 726042 Number of successful extensions: 2330 Number of sequences better than 1.0e-05: 192 Number of HSP's gapped: 2193 Number of HSP's successfully gapped: 192 Length of query: 283 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 178 Effective length of database: 26,268,361 Effective search space: 4675768258 Effective search space used: 4675768258 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)