BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0002.p (603 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH787 [H] COG1492 Cobyric acid synthase 400 e-111 FN0977 [H] COG1492 Cobyric acid synthase 364 e-100 FN2070 [H] COG1492 Cobyric acid synthase 325 2e-88 CC1751 [H] COG1492 Cobyric acid synthase 323 4e-88 CC2694 [H] COG1492 Cobyric acid synthase 315 1e-85 >MTH787 [H] COG1492 Cobyric acid synthase Length = 211 Score = 400 bits (1029), Expect = e-111, Method: Compositional matrix adjust. Identities = 0/502 (0%), Positives = 0/502 (0%), Gaps = 30/502 (5%) Query: 1 MIQGTASDVGKSLVTAAIGRIMTRDGYRTAPFKSQNMALNSYVTEDGREIGRAQGMQAEA 60 T S I +++ + K+ T +G++ Sbjct: 17 NPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYI 76 Query: 61 FGITATTDMNPILLKPSGEMSAQIVVHGVPHTALSAREYREKFLPEAKSTVMDALGRLRA 120 I ++K + Q A A Sbjct: 77 KTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIA 136 Query: 121 AYDIVLMEGAGSPAEINLKARDIVNMNLAGWADAPVLLVADIDRGGVFAFLVGTLELLEP 180 + + + L + + Sbjct: 137 VDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGS 196 Query: 181 HERARVKGFIINKFRGDLSLLQPGLDWLEQRTGIPVLGVLPFLPRLRIEAEDSVVLEGTS 240 Sbjct: 197 --- 253 Query: 241 GRLHAERGKELDVAVIRYPRISNFTDFDPLEDEPDTAVRYVSTAVELGTPDVIILPGTKN 300 Sbjct: 254 - 312 Query: 301 TAADLDYLREQGFPEAIAAALNQGTRQLA--GICGGYQMLGLKLLDPHAVESAEPGEREG 358 Sbjct: 313 ----- 367 Query: 359 LGFLPLSTSFLRQ-KTTVRATGLLAQDHPLPLYGGEAASATAAELPVEGYEIHMGTTTNH 417 Sbjct: 368 --------- 418 Query: 418 DPASVRSLFQLKGPDGSMQQEGW-GTPEGRVWGTYLHGLFHNDALRRAWLDGLRQSKGLA 476 Sbjct: 419 --- 475 Query: 477 P---LGQTFSAAALREQEFDRL 495 Sbjct: 476 -- 495 >FN0977 [H] COG1492 Cobyric acid synthase Length = 496 Score = 364 bits (934), Expect = e-100, Method: Compositional matrix adjust. Identities = 204/501 (40%), Positives = 287/501 (57%), Gaps = 37/501 (7%) Query: 1 MIQGTASDVGKSLVTAAIGRIMTRDGYRTAPFKSQNMALNSYVTEDGREIGRAQGMQAEA 60 MI GT+S GKSL A+ RI +D Y+ +PFKSQNMALNSY+T+DG+E+GRAQ +QAEA Sbjct: 7 MIVGTSSGAGKSLFVTALCRIFYKDKYKVSPFKSQNMALNSYITKDGKEMGRAQVVQAEA 66 Query: 61 FGITATTDMNPILLKPSGEMSAQIVVHGVPHTALSAREY---REKFLPEAKSTVMDALGR 117 G +MNPILLKPS QI+V G +S EY ++ +P K T + Sbjct: 67 SGTEPNVNMNPILLKPSTINKIQIIVCGKSIGNMSGVEYNQYKKNLIPILKET----YSK 122 Query: 118 LRAAYDIVLMEGAGSPAEINLKARDIVNMNLAGWADAPVLLVADIDRGGVFAFLVGTLEL 177 + DIV++EGAGSPAEIN+K DI N +A ADAPV+LVADIDRGGVFA + GT+ L Sbjct: 123 IENKNDIVVIEGAGSPAEINIKEEDISNFAMARIADAPVILVADIDRGGVFASIYGTIML 182 Query: 178 LEPHERARVKGFIINKFRGDLSLLQPGLDWLEQRTGIPVLGVLPFLPRLRIEAEDSVVLE 237 L +R R+KG +INKFRG+ +L+PG + +E TG+ LGV+P+ + IE EDS+ + Sbjct: 183 LHEEDRKRIKGIVINKFRGNKEVLKPGFEIIENLTGVKTLGVIPY-TDIDIEDEDSLSEK 241 Query: 238 GTSGRLHAERGKELDVAVIRYPRISNFTDFDPLEDEPDTAVRYVSTAVELGTPDVIILPG 297 S +L+ K + ++VI+ ISN TD D L D +++V+ ++G D II+PG Sbjct: 242 YKSFKLNKNSNK-IKISVIKLKHISNATDIDALSIYNDIEIQFVTERSQIGNEDFIIIPG 300 Query: 298 TKNTAADLDYLREQGFPEAIAAALNQGTRQLAGICGGYQMLGLKLLDPHAVESAEPGERE 357 +KNT DL +L+E G + I T + GICGG+Q+LG K+ DP+ +E + E Sbjct: 301 SKNTIDDLKWLKESGIAKEIIKRARTET-IIFGICGGFQILGNKVKDPYHIE-GDIEELN 358 Query: 358 GLGFLPLSTSFLRQKTTVRATGLLAQDHPLPLYGGEAASATAAELPVEGYEIHMGTTTNH 417 GLG L L T +KT V+ G L D+ + T + ++GYEIH G T + Sbjct: 359 GLGLLDLETIMENEKTLVQYKGKLVVDNGIL--------KTLNDFEIKGYEIHQGITKGN 410 Query: 418 DP---ASVRSLFQLKGPDGSMQQEGWGTPEGRVWGTYLHGLFHNDALRRAWLDGLRQSKG 474 + R++F + TYLHG+F N L+ +R+ KG Sbjct: 411 EKNLTTDERTIF---------------VNRDNIIATYLHGIFDNKDFTNTLLNEIRRRKG 455 Query: 475 LAPLGQTFSAAALREQEFDRL 495 L + S + +EFD+L Sbjct: 456 LEEVNDNISYEEYKLKEFDKL 476 >FN2070 [H] COG1492 Cobyric acid synthase Length = 491 Score = 325 bits (832), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 190/502 (37%), Positives = 284/502 (56%), Gaps = 41/502 (8%) Query: 1 MIQGTASDVGKSLVTAAIGRIMTRDGYRTAPFKSQNMALNSYVTEDGREIGRAQGMQAEA 60 MIQGT S VGK+L+ A + R+ +DGYR +PFKSQNMALNS+V +G E+ R +QAEA Sbjct: 8 MIQGTGSSVGKTLIVAGLCRVFVQDGYRVSPFKSQNMALNSFVDIEGLELSRGTVIQAEA 67 Query: 61 FGITATTDMNPILLKPSGEMSAQIVVHG-VPHTALSAREYREKFLPEAKSTVMDALGRLR 119 MNPILLKP+ + ++Q++++G + +TA A++Y K + + Sbjct: 68 GYEIPRAFMNPILLKPNSDNNSQVIINGKIAYTA-DAKKYFSNSKELKKIALETYKNNIE 126 Query: 120 AAYDIVLMEGAGSPAEINLKARDIVNMNLAGWADAPVLLVADIDRGGVFAFLVGTLELLE 179 +DI ++EG GSPAEINL+ D+VNM +A D+PV+LV +ID GGVFA + GT+ LL+ Sbjct: 127 NNFDIAVLEGGGSPAEINLREYDLVNMGMAELVDSPVILVGNIDIGGVFASIYGTVMLLD 186 Query: 180 PHERARVKGFIINKFRGDLSLLQPGLDWLEQR-----TGIPVLGVLPFLPRLRIEAEDSV 234 ++R R+KG+IINKFRGD LL+P +D L+++ I LGVLP+ L+IE EDS Sbjct: 187 ENDRKRIKGYIINKFRGDSDLLKPAIDILDKKFRAEGLDIKFLGVLPY-ADLKIEEEDS- 244 Query: 235 VLEGTSGRLHAERGKELDVAVIRYPRISNFTDFDPLEDEPDTAVRYVSTAVELGTPDVII 294 L R+++ K ++++VI+ ++SNFTDF + D VRYV +LG D+I+ Sbjct: 245 -LSDEDKRVYSNDKKYINISVIKTKKMSNFTDFHAFKQYDDVRVRYVYDVKDLGEEDIIV 303 Query: 295 LPGTKNTAADLDYLREQGFPEAIAAALNQGTRQLAGICGGYQMLGLKLLDPHAVESAEPG 354 PG+KNT DL+ L+++G E + L + + + GICGG QMLG K+ DP +ES + Sbjct: 304 FPGSKNTITDLEDLKKRGIFEKV-KELKEKGKIIIGICGGLQMLGKKIFDPKHLES-DIL 361 Query: 355 EREGLGFLPLSTSFLRQKTTVRATGLLAQDHPLPLYGGEAASATAAELPVEGYEIHMGTT 414 E EG F T+F K T + T + + G E ++GYEIH G T Sbjct: 362 ETEGFNFFDYETTFDEIKKTEQVT------KKIEVTEGILKDFNGYE--IKGYEIHQGVT 413 Query: 415 TNHDPASVRSLFQLKGPDGSMQQEGWGTPEGRVWGTYLHGLFHNDALRRAWLDGLRQSKG 474 + V+ TY+HG+F N +L+ +R+ K Sbjct: 414 DISTSVICKD---------------------NVFATYIHGVFDNSKFTNDFLNMIRREKN 452 Query: 475 LAPLGQTFSAAALREQEFDRLG 496 + +T S +E+E+++L Sbjct: 453 MPEQKETLSFNEFKEREYNKLA 474 >CC1751 [H] COG1492 Cobyric acid synthase Length = 211 Score = 323 bits (828), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 0/461 (0%), Positives = 0/461 (0%), Gaps = 40/461 (8%) Query: 1 MIQGTASDVGKSLVTAAIGRIMTRDGYRTAPFKSQNMALNSYVTEDGREIGRAQGMQAEA 60 + + ++ + A + V + ++ A Sbjct: 5 KRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAM 64 Query: 61 FGITATTDMNPILLKPSGEMSAQIVVHGVPHTALSAREYRE---KFLPEAKSTVMDALGR 117 + I A+ ++ L + + Sbjct: 65 LELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALE----ALPGVGRK 120 Query: 118 LRAAYDIVLMEGAGSPAEINLKARDIVNMNLAGWADAPVLLVADIDRGGVFAFLVGTLEL 177 + ++ G V F + Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLI 180 Query: 178 LEPHERARVKGFIINKFRGDLSLLQPGLDWLEQRTGIPVLGVLPFLPRL-RIEAEDSVVL 236 Sbjct: 181 240 Query: 237 EGTSGRLHAERGKELDVAVIRYPRISNFTDFDPLEDEPDTAVRYVSTAVEL-GTPDVIIL 295 Sbjct: 241 ---- 296 Query: 296 PGTKNTAADLDYLREQGFPEAIAAALNQGTRQLAGICGGYQMLGLKLLDPHAVESAEPGE 355 Sbjct: 297 -- 354 Query: 356 REGLGFLPLSTSFLRQKTTVRATGLLAQDHPLPLYGGEAASATAAELPVEGYEIHMGTTT 415 Sbjct: 355 ----------------- 397 Query: 416 NHDPASVRSLFQLKGPDGSMQQEGWGTPEGRVWGTYLHGLF 456 Sbjct: 398 -------- 430 >CC2694 [H] COG1492 Cobyric acid synthase Length = 484 Score = 315 bits (807), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 204/471 (43%), Positives = 268/471 (56%), Gaps = 40/471 (8%) Query: 1 MIQGTASDVGKSLVTAAIGRIMTRDGYRTAPFKSQNMALNSYVTEDGREIGRAQGMQAEA 60 MIQG SDVGKS++ A + R+ G+ PFK QNM+ N+ VT +G EIGRAQ +QA A Sbjct: 5 MIQGCGSDVGKSVLVAGLCRLFANRGFAVRPFKPQNMSNNAAVTAEGGEIGRAQALQAIA 64 Query: 61 FGITATTDMNPILLKPSGEMSAQIVVHGVPHTALSAREYRE---KFLPEAKSTVMDALGR 117 T +MNP+LLKP ++ AQ+VV G + +A Y++ LP V+++ Sbjct: 65 CRTPPTVEMNPVLLKPQSDIGAQVVVRGRMAGSWAASGYQDHKASLLP----IVVESFRA 120 Query: 118 LRAAYDIVLMEGAGSPAEINLKARDIVNMNLAGWADAPVLLVADIDRGGVFAFLVGTLEL 177 L D+V++EGAGSPAEINL+A DI NM A AD PV+LV DIDRG V A LVG + Sbjct: 121 LERQSDLVIVEGAGSPAEINLRAGDIANMGFARAADVPVVLVGDIDRGHVIAALVGAHAV 180 Query: 178 LEPHERARVKGFIINKFRGDLSLLQPGLDWLEQRTGIPVLGVLPFLPRL-RIEAEDSVVL 236 L+P +RA +KGF+INKFRGD +L G + +RTG LG+ P+L R+ AED+VVL Sbjct: 181 LDPEDRAMIKGFLINKFRGDPALFDDGRRAIVERTGWADLGMAPWLAAARRLPAEDAVVL 240 Query: 237 EGTSGRLHAERGKELDVAVIRYPRISNFTDFDPLEDEPDTAVRYVSTAVEL-GTPDVIIL 295 + S A R + + V RI+NF +FD L EP A +V L G DV+IL Sbjct: 241 DARS----AGRDGRIRIVVPMLSRIANFDEFDALRAEPGVAFAFVPPGSALPGDADVVIL 296 Query: 296 PGTKNTAADLDYLREQGFPEAIAAALNQGTRQLAGICGGYQMLGLKLLDPHAVESAEPGE 355 PGTK T ADLD++R QG+ + A L + R L GICGGYQMLG ++ DP VE A PG Sbjct: 297 PGTKATRADLDFVRAQGWDIDLQAHLRRSGRVL-GICGGYQMLGRRVADPDGVEGA-PGT 354 Query: 356 REGLGFLPLSTSFLRQKTTVRATGLLAQDHPLPLYGGEAASATAAELPVEGYEIHMGTTT 415 EGLG L + T KT TG L GG EGYE+H+G T+ Sbjct: 355 SEGLGLLDVETVMTGDKTLRPVTGRLP--------GGAR---------FEGYEMHVGRTS 397 Query: 416 NHDPASVRSLFQLKGPDGSMQQEGWGTPEGRVWGTYLHGLFHNDALRRAWL 466 + R + + + +G + +GRV G Y+HGLF R A L Sbjct: 398 G----AARPMLTFD----TGETDGAVSADGRVSGCYVHGLFDRGEARAALL 440 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 46,889,947 Number of extensions: 2194035 Number of successful extensions: 5620 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 5592 Number of HSP's successfully gapped: 7 Length of query: 603 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 491 Effective length of database: 25,461,023 Effective search space: 12501362293 Effective search space used: 12501362293 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)