BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0013.p (324 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3601 [M] COG2222 Predicted phosphosugar isomerases 475 e-134 STM0572 [M] COG2222 Predicted phosphosugar isomerases 313 3e-85 SMc03139 [M] COG2222 Predicted phosphosugar isomerases 123 4e-28 STM4540 [M] COG2222 Predicted phosphosugar isomerases 113 4e-25 yhfN [M] COG2222 Predicted phosphosugar isomerases 100 2e-21 >STM3601 [M] COG2222 Predicted phosphosugar isomerases Length = 325 Score = 475 bits (1223), Expect = e-134, Method: Compositional matrix adjust. Identities = 216/324 (66%), Positives = 264/324 (81%) Query: 1 MDIKQIIEQITADKKEAGGIKQVYYAACGGSYGAFYPAKTFLEKEAKDIKVGLYNSNEFV 60 M +K+ + I + E GG+K VYY ACGGSY AFYPAK FLEKEAK + VGLYNS EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGVKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 61 HNTPKAFGENSVLVVASHKGNTPETVKAAELGKKAGVPVIALTWIEDSPITEYADYVVKY 120 +N P A GEN+V+VVASHKGNTPET+KAAE+ ++ G PVI LTWI DSP+ + DYV Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETY 121 Query: 121 TFGENKDIAGEKTIQALMTAVEILKQTEGYGNYDKFLDGVSRIDRIVKQACKHVANRALT 180 TFG+ KDIAGEKT++ L++AVE+L+QTEGY +YD F DGVS+I+RIV +AC+ VA RA Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 181 FARNHKDDSVIYTMASXXXXXXXXMESICIFMEMQWINSASIHSGEYFHGPFEITDAEIP 240 FA+ +KDD VIYT+AS ++SICIFMEMQWI+SA IHSGE+FHGPFEITDA P Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 241 FVIQVSEGSTRALDERALSFLNRYAKRVEVLDAKDLGLSTIDSSVVDYFNHSLFNNVYPV 300 F Q SEG+TRA+DERAL+FL +Y +R+EV+DAK+LGLSTI ++V+DYFNHSLFNNVYPV Sbjct: 242 FFFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 301 YNQALAVEREHPLTTRRYMWKVEY 324 YN+ALA R+HPLTTRRYMWKVEY Sbjct: 302 YNRALAEARQHPLTTRRYMWKVEY 325 >STM0572 [M] COG2222 Predicted phosphosugar isomerases Length = 328 Score = 313 bits (802), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 153/324 (47%), Positives = 204/324 (62%), Gaps = 7/324 (2%) Query: 4 KQIIEQITADKKEAGGIKQVYYAACGGSYGAFYPAKTFLEKEAKDIKVGLYNSNEFVHNT 63 ++II I + I++V++ CGGS F+P K FL+ EA + VG SNEFVH T Sbjct: 9 RRIISDILGKQN----IERVWFVGCGGSLTGFWPGKYFLDCEASKLAVGYITSNEFVHAT 64 Query: 64 PKAFGENSVLVVASHKGNTPETVKAAELGKKAGVPVIALTWIEDSPITEYADYVVKYTFG 123 PKA G+NSV+++AS +GNT ETV AA + ++ G I L + D+P+ EY+DY+++Y + Sbjct: 65 PKALGKNSVVILASQQGNTAETVAAARVAREKGAATIGLVYQPDTPLCEYSDYIIEYQWA 124 Query: 124 ---ENKDIAGEKTIQALMTAVEILKQTEGYGNYDKFLDGVSRIDRIVKQACKHVANRALT 180 E D A +K +L A+EIL QTEGY YD+ + R +V A + V A Sbjct: 125 RYPETVDPAQQKAAYSLWLALEILAQTEGYAQYDELVSAFGRFSDVVHGAQRQVQEDAQR 184 Query: 181 FARNHKDDSVIYTMASXXXXXXXXMESICIFMEMQWINSASIHSGEYFHGPFEITDAEIP 240 FA KD+ V+Y M S ESICI +EMQWINSASIHSGEYFHGPFEIT+ P Sbjct: 185 FAAEWKDEKVVYMMGSGPSFGAAHQESICILLEMQWINSASIHSGEYFHGPFEITEPGTP 244 Query: 241 FVIQVSEGSTRALDERALSFLNRYAKRVEVLDAKDLGLSTIDSSVVDYFNHSLFNNVYPV 300 F++ S G TR LD+RA+ F+ RY +++++DA LG+ + + V +YF L N V V Sbjct: 245 FILLQSSGRTRPLDDRAIRFIERYQGKLQLIDADKLGIQDLSTDVGEYFCGLLHNCVLDV 304 Query: 301 YNQALAVEREHPLTTRRYMWKVEY 324 YN ALA R HPLTTRRYMWKVEY Sbjct: 305 YNLALATARNHPLTTRRYMWKVEY 328 >SMc03139 [M] COG2222 Predicted phosphosugar isomerases Length = 337 Score = 123 bits (308), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 84/327 (25%), Positives = 148/327 (45%), Gaps = 13/327 (3%) Query: 3 IKQIIEQITADKKEAGGIKQVYYAACGGSYGAFYPAKTFLEKEAK-----DIKVGLYNSN 57 ++Q ++ + E G + V++ GG+ PA L++ ++ D+ L+ + Sbjct: 19 LRQRLDDVVVSCLEKGA-ENVFFLGTGGAAILMQPAAQLLQRRSRFPAFIDLPAELFITG 77 Query: 58 EFVHNTPKAFGENSVLVVASHKGNTPETVKAAELGKKAGVPVIALTWIEDSPITEYADYV 117 V+ T E S++V+ S G T E+V K+AG V++L E++P+ + D+ Sbjct: 78 S-VNLT-----EKSIVVIPSLSGTTKESVALLAKAKEAGATVLSLVGHEETPLGKGGDHA 131 Query: 118 VKYTFGENKDIAGEKTIQALMTAVEILKQTEGYGNYDKFLDGVSRIDRIVKQACKHVANR 177 F E+ +Q+L A+ I++ NYD+ + R+ ++ + + ++ Sbjct: 132 F-VNFAEDDTSCESFYLQSLFIALSIMRHRGEIENYDQIAGELERLPGLLLEVKRGYEDK 190 Query: 178 ALTFARNHKDDSVIYTMASXXXXXXXXMESICIFMEMQWINSASIHSGEYFHGPFEITDA 237 A TFAR + +CI EMQWI + +H+ ++FHG E+ + Sbjct: 191 AETFARVLAGSDYHIVTGAGNAWPEAFYYGMCILEEMQWIRTRPVHASDFFHGTLELVEK 250 Query: 238 EIPFVIQVSEGSTRALDERALSFLNRYAKRVEVLDAKDLGLSTIDSSVVDYFNHSLFNNV 297 + ++ E + R L +R +F Y R+ VLD D L I V + + V Sbjct: 251 GVSVILFKGEDALRPLADRVQNFAPNYTDRLTVLDTADFALPGISQEVRGLVSPIVLATV 310 Query: 298 YPVYNQALAVEREHPLTTRRYMWKVEY 324 + L V R HPLTTRRY +V Y Sbjct: 311 LERISAHLEVMRNHPLTTRRYYKRVAY 337 >STM4540 [M] COG2222 Predicted phosphosugar isomerases Length = 343 Score = 113 bits (283), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 84/330 (25%), Positives = 146/330 (44%), Gaps = 22/330 (6%) Query: 4 KQIIEQITADKKEAGGIKQVYYAACGGSYGAFYPAKTFLEKEAKDIKVGLYNSNEFVHNT 63 +Q EQ+ AD+ G +++A+ GGS F KE + V + + E +H Sbjct: 27 RQKAEQV-ADEIYQAGFSSLFFASVGGSLAPMMAINEF-AKELTTLPVYVEQAAELIHKG 84 Query: 64 PKAFGENSVLVVASHKGNTPETVKAAELGKKAGVPVIALTWIEDSPITEYADYVVKYTFG 123 K ++SV++ S G+T E+V AE K G+ V+A+T DSP+ + A + + Sbjct: 85 NKRLNKDSVVITLSKSGDTKESVAIAEWCKAQGIRVVAITKNADSPLAQAATWHIPM--- 141 Query: 124 ENKDIAGEKTIQALMTAVEILKQTEGYGNYDKFLDGVSRIDRIVKQACKHVANRALTFAR 183 +K+ + + +L + + +YD+F + + + +A + +A A Sbjct: 142 RHKNGVEYEYMLLYWLFFRVLSRNNEFASYDRFASQLEILPANLLKAKQKFDPQADAIAS 201 Query: 184 NHKDDSVIYTMASXXXXXXXXMESICIFMEMQWINSASIHSGEYFHGPFEITDAEIPFVI 243 + + + + + S+CI EMQW + + S E+FHG E+ + ++P ++ Sbjct: 202 RYHNSDYMMWVGGAEMWGEVYLFSMCILEEMQWKRTRPVSSAEFFHGALELLEKDVPLIL 261 Query: 244 QVSEGSTRALDERALSFLNRYAKRVEVLDAKDLGLSTIDSSVVDYFNHSLFNNVYPVYNQ 303 EG RALDER F ++ + V+D K L ID D F + P Sbjct: 262 VKGEGKCRALDERVERFASKITDNLVVIDPKAYALDGID----DEFRWIM----APCVVS 313 Query: 304 ALAVER---------EHPLTTRRYMWKVEY 324 L V+R H L RRY + +Y Sbjct: 314 TLLVDRLAAHFEKYTGHSLDIRRYYRQFDY 343 >yhfN [M] COG2222 Predicted phosphosugar isomerases Length = 347 Score = 100 bits (250), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 67/254 (26%), Positives = 110/254 (43%), Gaps = 6/254 (2%) Query: 20 IKQVYYAACGGSYGAFYPAKTFLEKEAKDIKVGLYNSNEFVHNTPKAFGENSVLVVASHK 79 I ++Y+ ACG A AK ++ D++V + EF NTP + ++ S Sbjct: 51 IDRIYFVACGSPLNAAQTAKHLADR-FSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY 109 Query: 80 GNTPETVKAAELGKKAGVPVIALTWIEDSPITEYADYVVKYTFGENKDIAGEKTIQALMT 139 G T E +KA ELG+ G A T DSPIT A++ + Y D E + + Sbjct: 110 GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDY----QADCIWEIHLLLCYS 165 Query: 140 AV-EILKQTEGYGNYDKFLDGVSRIDRIVKQACKHVANRALTFARNHKDDSVIYTMASXX 198 V E++ + K + + ++ + + + +IYT+A+ Sbjct: 166 VVLEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP 225 Query: 199 XXXXXXMESICIFMEMQWINSASIHSGEYFHGPFEITDAEIPFVIQVSEGSTRALDERAL 258 E I ME W + I SGE+ HGP EI + +PF+ + +R ERA+ Sbjct: 226 LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTERAI 285 Query: 259 SFLNRYAKRVEVLD 272 +F+ + V V+D Sbjct: 286 NFVKQRTDNVIVID 299 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,191,226 Number of extensions: 927446 Number of successful extensions: 2914 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 2912 Number of HSP's successfully gapped: 16 Length of query: 324 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 217 Effective length of database: 26,037,693 Effective search space: 5650179381 Effective search space used: 5650179381 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)