BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0012.p (288 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC3498 [G] COG0524 Sugar kinases, ribokinase family 342 5e-94 STM3600 [G] COG0524 Sugar kinases, ribokinase family 250 2e-66 BS_yurL [G] COG0524 Sugar kinases, ribokinase family 196 3e-50 BS_yhfQ [P] COG4594 ABC-type Fe3+-citrate transport system, peri... 117 2e-26 Z4734m [G] COG0524 Sugar kinases, ribokinase family 115 7e-26 >CAC3498 [G] COG0524 Sugar kinases, ribokinase family Length = 295 Score = 342 bits (876), Expect = 5e-94, Method: Compositional matrix adjust. Identities = 157/289 (54%), Positives = 218/289 (75%), Gaps = 2/289 (0%) Query: 1 MKQYKVIGIGDNVVDKYVHQGIMYPGGNALNFSVYATMCGMESAYLGKFGSDEVAGYIRQ 60 MK YK++ IGDNV DKY+ +G MYPGG +N VY M G E+AY+GK+G+DEVA ++ Sbjct: 1 MKNYKIVAIGDNVCDKYLSRGKMYPGGQCINTCVYGKMNGAETAYIGKYGNDEVAECVQN 60 Query: 61 VIDELNIDRSCCREYEGENGYARVTLEDGDRVFLGSNKGGIAKEHAWQFTKKDLDYIKQF 120 + E+ ID S CR YEGENG+A VTL++ DRVFLGSNKGGIAKEH++ F + D +YIK+F Sbjct: 61 TLKEIGIDDSHCRHYEGENGFALVTLKNADRVFLGSNKGGIAKEHSYNFDENDFEYIKKF 120 Query: 121 AVIHTSLNSYIEQDLTVLRESGVPISYDFSVRWNDGYLEQVCPNVDISFLSCSHLTAEER 180 +I+T+LNSYIE +L L +GVPI+YDFS+RW D YL +VCP +D++ LSC+HLTAEER Sbjct: 121 DLIYTNLNSYIEDNLKELHATGVPIAYDFSMRWTDEYLLKVCPYIDVAILSCAHLTAEER 180 Query: 181 EREMRKAQRFGAKIVVGTVGENGSYALFSDQWIYHPAV-STEAVDTMGAGDSYITAFLVE 239 E+EM+K Q +G KIV+GT+GE+GSY L++ ++Y A+ + +DTMGAGDSY + FL Sbjct: 181 EKEMKKVQNYGVKIVLGTIGEDGSYVLYNGDFLYTSAIMAANIIDTMGAGDSYFSTFLCS 240 Query: 240 LIRNSD-GRIEFGQESEMLGKIKSSMSKGAEFAAEICRVNGAFGFGTPL 287 L++ S+ G++ G + M +++S+M GA FAA +C + GAFG+G P+ Sbjct: 241 LLQTSETGKLVEGTKESMKIRLESAMKSGAIFAANVCGMEGAFGYGVPI 289 >STM3600 [G] COG0524 Sugar kinases, ribokinase family Length = 281 Score = 250 bits (638), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 121/282 (42%), Positives = 182/282 (64%), Gaps = 7/282 (2%) Query: 6 VIGIGDNVVDKYVHQGIMYPGGNALNFSVYATMCGMESAYLGKFGSDEVAGYIRQVIDEL 65 V+GIGDNVVDKY+H GIMYPGGNALNF+VYA + + SA++G FG+D+ A +++ V+ +L Sbjct: 5 VLGIGDNVVDKYLHSGIMYPGGNALNFAVYAKLADIPSAFMGAFGNDDAAQHVQDVLHQL 64 Query: 66 NIDRSCCREYEGENGYARVTLEDGDRVFLGSNKGGIAKEHAWQFTKKDLDYIKQFAVIHT 125 ID S R Y GENGYA + L GDR F+ SNK G+ +EH + + DL YI QF ++H+ Sbjct: 65 QIDISHSRHYTGENGYACIRLSHGDRQFVASNKNGVLREHPFSLSDDDLRYISQFTLVHS 124 Query: 126 SLNSYIEQDLTVLRESGVPISYDFSVRWNDGYLEQVCPNVDISFLSCSHLTAEEREREMR 185 S+N ++E +L +++ V +S+DFS R D Y E+VCP VD F+SCS L+ +E + ++ Sbjct: 125 SINGHLESELEKIKQQTVLLSFDFSGRGTDDYFEKVCPWVDYGFISCSGLSPDEIKVKLN 184 Query: 186 KAQRFGAKIVVGTVGENGSYALFSDQWIYHPAVSTEAVDTMGAGDSYITAFLVELIRNSD 245 K R+G + ++ T G Y ++ E VDT+GAGD+++T FL+ ++++ Sbjct: 185 KLYRYGCRHIIATCGHEKVYYFSGADYLEWQPAYIEPVDTLGAGDAFLTGFLLSILQS-- 242 Query: 246 GRIEFGQESEMLGKIKSSMSKGAEFAAEICRVNGAFGFGTPL 287 G E +ES + +M +G + AA++ GAFGFG P Sbjct: 243 GMAEPDKES-----VLRAMRQGGKSAAQVLSHYGAFGFGKPF 279 >BS_yurL [G] COG0524 Sugar kinases, ribokinase family Length = 284 Score = 196 bits (499), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 115/283 (40%), Positives = 156/283 (55%), Gaps = 16/283 (5%) Query: 5 KVIGIGDNVVDKYVHQGIMYPGGNALNFSVYATMCGMESAYLGKFGSDEVAGYIRQVIDE 64 K+I +GDNVVD Y Q YPGGNALN +V A G ES+Y+G G+DE A ++ V+ Sbjct: 2 KLIAVGDNVVDYYQDQETFYPGGNALNVAVLAKRLGHESSYIGIVGNDEAAAHLLNVLKL 61 Query: 65 LNIDRSCCREYEGENGYARVTL-EDGDRVFLGSNKGGIAKEHAWQFTKKDLDYIKQFAVI 123 ++ R+ GENG A VTL E GDR+F+ SNKGGI F +KD+ +I ++ Sbjct: 62 EQVNADYIRQAHGENGMAIVTLDEQGDRIFVRSNKGGIQSRLRLAFQEKDVSFISGHDLL 121 Query: 124 HTSLNSYIEQDLTVLRESGVPISYDFSVRWNDGYLEQVCPNVDISFLSCSHLTAEERERE 183 HTS+ S +E DL L VP+S+DFS D YL +VCP V +F S S L+ E Sbjct: 122 HTSVYSRLENDLPQLC-GLVPVSFDFSTNREDDYLRRVCPYVTYAFFSGSDLSESECGEL 180 Query: 184 MRKAQRFGAKIVVGTVGENGSYALFSDQWIYHPAVSTEAVDTMGAGDSYITAFLVELIRN 243 + A +GAK+V T G G+ D+ + P V + +DT+GAGDS+I FL Sbjct: 181 AKTAHGYGAKMVCMTRGGQGAILSAGDRVYHQPIVEADIIDTLGAGDSFIAGFLTAFCVK 240 Query: 244 SDGRIEFGQESEMLGKIKSSMSKGAEFAAEICRVNGAFGFGTP 286 D I ++ + AE AA+ C V GAFG+G P Sbjct: 241 QD--------------ITYALRQAAETAAKTCGVYGAFGYGYP 269 >BS_yhfQ [P] COG4594 ABC-type Fe3+-citrate transport system, periplasmic component Length = 261 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 77/273 (28%), Positives = 130/273 (47%), Gaps = 18/273 (6%) Query: 9 IGDNVVDKYVHQGIMYPGGNALNFSVYATMCGMESAYLGKFGSDEVAGYIRQVIDELNID 68 IGDN VD Y + GGNA+N +VY T G++ + G D+ ++Q + + +D Sbjct: 7 IGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCITWVGDDDYGTKLKQDLARMGVD 66 Query: 69 RSCCREYEGENGYARVTLEDGDRVFLGSNKGGIAKEHAWQFTKKDLDYIKQFAVIHTSLN 128 S G +V L D DRVF G G+ + A +++D ++ Q+ ++H ++ Sbjct: 67 ISHVHTKHGVTAQTQVELHDNDRVF-GDYTEGVMADFA--LSEEDYAWLAQYDIVHAAIW 123 Query: 129 SYIEQDLTVLRESGVPISYDFSVRWNDGYLEQVCPNVDISFLSCSHLTAEEREREMRKAQ 188 + E L +G ++DFS +W+ + + P++D +F S E +M+ Sbjct: 124 GHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHLDFAFASAPQ-EDETLRLKMKAIV 182 Query: 189 RFGAKIVVGTVGENGSYALFSDQWIYHPAVSTEAVDTMGAGDSYITAFLVELIRNSDGRI 248 GA V+ T+GENGS A Q+ +DTMGAGDS+I FL Sbjct: 183 ARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVIDTMGAGDSFIAGFLC---------- 232 Query: 249 EFGQESEMLGKIKSSMSKGAEFAAEICRVNGAF 281 G + M + ++++G AA+ + +GA+ Sbjct: 233 --GWSAGM--TLPQAIAQGTACAAKTIQYHGAW 261 >Z4734m [G] COG0524 Sugar kinases, ribokinase family Length = 262 Score = 115 bits (289), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 77/273 (28%), Positives = 129/273 (47%), Gaps = 18/273 (6%) Query: 9 IGDNVVDKYVHQGIMYPGGNALNFSVYATMCGMESAYLGKFGSDEVAGYIRQVIDELNID 68 IGDN VD Y + GGNA+N +VY T G++ + G D+ ++Q + + +D Sbjct: 8 IGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCITWVGDDDYGTKLKQDLASMGVD 67 Query: 69 RSCCREYEGENGYARVTLEDGDRVFLGSNKGGIAKEHAWQFTKKDLDYIKQFAVIHTSLN 128 S G +V L D DRVF G G+ + A +++D + Q+ ++H ++ Sbjct: 68 ISHVHTKHGVTAQTQVELHDNDRVF-GDYTEGVMADFA--LSEEDYAXLAQYDIVHAAIW 124 Query: 129 SYIEQDLTVLRESGVPISYDFSVRWNDGYLEQVCPNVDISFLSCSHLTAEEREREMRKAQ 188 + E L +G ++D S +W+ + + P++D +F S E +M+ Sbjct: 125 GHAEDAFPQLHAAGKLTAFDVSDKWDSPLWQTLVPHLDFAFASAPQ-EDEALRLKMKAIV 183 Query: 189 RFGAKIVVGTVGENGSYALFSDQWIYHPAVSTEAVDTMGAGDSYITAFLVELIRNSDGRI 248 GA +V+ T+GENGS A Q+ +DTMGAGDS+I FL Sbjct: 184 ARGAGVVIVTLGENGSIAWDGVQFWRQAPEQVTVIDTMGAGDSFIAGFLC---------- 233 Query: 249 EFGQESEMLGKIKSSMSKGAEFAAEICRVNGAF 281 G + M + +M++G AA+ + +GA+ Sbjct: 234 --GWSAGM--TLPQAMAQGTACAAQTIQYHGAW 262 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,034,931 Number of extensions: 1021134 Number of successful extensions: 2988 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 2980 Number of HSP's successfully gapped: 22 Length of query: 288 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 183 Effective length of database: 26,268,361 Effective search space: 4807110063 Effective search space used: 4807110063 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)