BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0011.p (450 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM3599 [R] COG2704 Anaerobic C4-dicarboxylate transporter 358 1e-98 PM1434 [R] COG2704 Anaerobic C4-dicarboxylate transporter 253 5e-67 HI0746 [R] COG2704 Anaerobic C4-dicarboxylate transporter 250 4e-66 PM1918 [R] COG2704 Anaerobic C4-dicarboxylate transporter 241 2e-63 VCA0205 [R] COG2704 Anaerobic C4-dicarboxylate transporter 236 6e-62 >STM3599 [R] COG2704 Anaerobic C4-dicarboxylate transporter Length = 289 Score = 358 bits (919), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 0/400 (0%), Positives = 0/400 (0%), Gaps = 1/400 (0%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 M G + + + + + + I Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 ++ +A + S + T L + Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 + + + S + + + +L Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVESAPEEQPEKKATPKGAKXXXXXXXXXXXXX 240 + + K E + + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEY-VMFTYEGLGTGVQEF 239 Query: 241 XXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRAGA 300 Sbjct: 240 299 Query: 301 LAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTLIM 360 Sbjct: 300 359 Query: 361 IPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD 400 Sbjct: 360 399 >PM1434 [R] COG2704 Anaerobic C4-dicarboxylate transporter Length = 289 Score = 253 bits (646), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 0/403 (0%), Positives = 0/403 (0%), Gaps = 6/403 (1%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 M G + + + + + + I Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 ++ +A + S + T L + Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 + + + S + + + +L Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 181 IVSALLTLRKGKELAEDPEYLRRLA--EGLVESAPEEQPEKKATPKGAKXXXXXXXXXXX 238 + + K E +G V + + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFI 240 Query: 239 XXXXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRA 298 P L T Sbjct: 241 LTVYGTCMPMLNL--TRRK-- 296 Query: 299 GALAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTL 358 Sbjct: 297 356 Query: 359 IMIPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFDT 401 Sbjct: 357 399 >HI0746 [R] COG2704 Anaerobic C4-dicarboxylate transporter Length = 289 Score = 250 bits (638), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 0/403 (0%), Positives = 0/403 (0%), Gaps = 6/403 (1%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 + + + G +F + Sbjct: 4 AGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSA 63 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 + ++ S P+ Sbjct: 64 VALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEF 123 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLGVGLGTILSIAIPATLLGV 180 + +CS + LG +I L Sbjct: 124 TMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNE 183 Query: 181 IVSALLTLRKGKELAEDPEYLRRLAEGLVES--APEEQPEKKATPKGAKXXXXXXXXXXX 238 + + + + ++ + E Sbjct: 184 TRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTV 243 Query: 239 XXXXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNGTIFRA 298 + R Sbjct: 244 YGTCMPMLNLTRRKG--QD-- 299 Query: 299 GALAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQGATTL 358 Sbjct: 300 359 Query: 359 IMIPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFDT 401 Sbjct: 360 402 >PM1918 [R] COG2704 Anaerobic C4-dicarboxylate transporter Length = 289 Score = 241 bits (614), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 0/422 (0%), Positives = 0/422 (0%), Gaps = 13/422 (3%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 M G + + + + + + I Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 ++ +A + S + T L + Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPL----GVGLGTILSIAIPAT 176 + + + S + + Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 177 LLGVIVSALLTLRKGKELAEDPEYLRRLAEGLVESA---PEEQPEKKATPKGAKXXXXXX 233 L S + + L + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFI 240 Query: 234 XXXXXXXXXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNG 293 + Sbjct: 241 LTVYGTCMPMLNLTRRK--- 297 Query: 294 TIFRAGALAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQ 353 Sbjct: 298 357 Query: 354 GATTLIMIPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFD---TXXXGGNHQN 410 Sbjct: 358 417 Query: 411 RQ 412 Sbjct: 418 419 >VCA0205 [R] COG2704 Anaerobic C4-dicarboxylate transporter Length = 289 Score = 236 bits (602), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 0/408 (0%), Positives = 0/408 (0%), Gaps = 12/408 (2%) Query: 1 MFWLEXXXXXXXXXXGARVGDVFMGLLGGVGVAIFVFIFHVQPASPPVDVMLIIXXXXXX 60 M G + + + + + + I Sbjct: 1 MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS 60 Query: 61 XXXXXXXXXXNFLVHVAEKLLHKSPKNITIVAPVICWLFTFLSGTGHVVYSLLPIINKLA 120 ++ +A + S + T L + Sbjct: 61 KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRL 120 Query: 121 IDSGIRPERPXXXXXXXXXXXXTCSPVSAATAAMLGLMAPLG--VGLGTILSIAIPATLL 178 + + + S + P Sbjct: 121 GEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYG 180 Query: 179 GVIVSALLTLRKGKELAEDPEYLRRLA-----EGLVESAPEEQPEKKATPKGAKXXXXXX 233 +E+ + A G + Sbjct: 181 LNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYV--MFTYEGLGTGVQE 238 Query: 234 XXXXXXXXXXGLFPDLRPSWTVDGVTTVLTMAHTIEIIMLVCASLIIIICRPAIETILNG 293 + R Sbjct: 239 FILTVYGTCMPMLNLTR--- 295 Query: 294 TIFRAGALAMVCAFGLAWMSNTFIGGQTEFIQEHVAAVVNSQPWMLAVIMFIVAALTTSQ 353 Sbjct: 296 355 Query: 354 GATTLIMIPIAIGLDLPAYIIVGAWMAVNANFFLPVSAQCLAAISFDT 401 Sbjct: 356 403 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,107,801 Number of extensions: 1000567 Number of successful extensions: 3216 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 3162 Number of HSP's successfully gapped: 22 Length of query: 450 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 340 Effective length of database: 25,691,691 Effective search space: 8735174940 Effective search space used: 8735174940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)