BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0002.p (359 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L0241 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 357 2e-98 mll4920 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 107 3e-23 YPO1280 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 107 4e-23 SMc01501 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 104 2e-22 ZyeiQ [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 102 8e-22 >L0241 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 544 Score = 357 bits (916), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 179/363 (49%), Positives = 233/363 (64%), Gaps = 4/363 (1%) Query: 1 MSIVASLAYKRYLKGAFPMTFVSMDNCSHNGDKLKKGVVTIAKEWTARGLVEEGFVAYLE 60 M V L Y R+ G FP+ VS DN S NG KL++ ++TIAK W +VE+ F YL Sbjct: 176 MGAVTYLLYARFKAGKFPIAMVSTDNFSENGLKLQEAILTIAKAWVENEIVEQDFYDYLI 235 Query: 61 NEQLIAFPLSMIDKITPRPSETVQAALLEQGIGGMDVIITSKNTYTAPFVNAEISEYLVI 120 N + ++FP SMID+ITP PSE V L G +++ + K+T APF N E YLVI Sbjct: 236 NPKKVSFPWSMIDRITPNPSEKVAKLLTADGFEDTEILHSQKHTNIAPFGNTEEVHYLVI 295 Query: 121 EDKFTNGRPLLEEAGIIFTDRDTVNNVETMKVTTCLNPLHTALAVSGCLLGYSLIADEMK 180 ED F NGRP LE++G+I TDR+TVN+ + MKVT CLNPLHTALA+ G LL Y I +E+ Sbjct: 296 EDAFPNGRPALEKSGVILTDRETVNDADQMKVTACLNPLHTALAIFGSLLDYHSIWEEVA 355 Query: 181 DDTLRRFVEIIGYKEGLPVVVDPGILSPKQFLDEVLTERFANPFIPDTPQRIATDTSQKV 240 + L ++ +GY E LPVV +P I++PK F+D++LT+R N IPDTPQRIA DTSQK+ Sbjct: 356 NPDLLALIKNLGYGEALPVVKNPKIINPKDFIDQLLTKRLPNKNIPDTPQRIAADTSQKI 415 Query: 241 GIRFGETIKSYIRREELDPAQLTAIPLAIAVWCRYLLGVNDEGEAFTLSPDPLLETLQGH 300 +R+G TI YI +L IPL IA WCRYL+G+NDE E+F+ SPDPLLE LQ Sbjct: 416 PVRYGVTIGHYIANPRFSVKELEFIPLVIAAWCRYLIGINDELESFSPSPDPLLEELQAF 475 Query: 301 LQGVALGDRNASV----RPILEDEQLFGVPLYEIGLGSKIEGMFLEMLAGPGAVRRTLEK 356 + V L + +PIL + Q+F +Y+ GL KIE F +ML G GAV TL+ Sbjct: 476 VADVKLDGSQQEIHEILKPILSNHQIFPNEMYQTGLAQKIENYFEQMLQGKGAVLNTLQN 535 Query: 357 YLK 359 LK Sbjct: 536 VLK 538 >mll4920 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 492 Score = 107 bits (267), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 88/302 (29%), Positives = 145/302 (48%), Gaps = 36/302 (11%) Query: 1 MSIVASLAYKRYLKGAFPMTFVSMDNCSHNGDKLKKGVVTIAKEWTARGLVEEGFVAYLE 60 I+ + +R +G P T +S DN HNG GV+ +A+ L++E ++ Sbjct: 166 FGIILAGLLRRRDEGTVPFTVMSCDNIPHNGHVTSDGVIGLAR------LIDEDLAGWVS 219 Query: 61 NEQLIAFPLSMIDKITPRPSETVQAALLEQGIGGMDVII-TSKNTYTAPFVNAEISEYLV 119 + +AFP M+D+ITP ++ E+GI D + + + PF V Sbjct: 220 SN--VAFPNGMVDRITPATTDR------ERGILAKDFGVEDAWPVFCEPF------RQWV 265 Query: 120 IEDKFTNGRPLLEEAGIIFTDRDTVNNVETMKVTTCLNPLHTALAVSGCLLGYSLIADEM 179 +ED FT+GRP LE+ G+ F RD V E MK+ LN H +A L+ + + M Sbjct: 266 LEDHFTDGRPPLEKVGVQFV-RD-VAPYELMKI-RILNGGHATIAYPAGLMDIHFVHEAM 322 Query: 180 KDDTLRRFVEIIGYKEGLPVVVDPGILSPKQFLD---EVLTERFANPFIPDTPQRIATDT 236 ++ +R F++ + + E +P V P + P L+ +++ +RF+NP I DT +R+ D Sbjct: 323 QEPLVRGFLDKLEHDEIIPTV--PPV--PDTVLEDYYQLIEKRFSNPKIGDTIRRLCLDG 378 Query: 237 SQKVGIRFGETIKSYIRREELDPAQLTAIPLAIAVWCRYLLGVNDEGEAFTLSPDPLLET 296 S + TI ++ + + + L A+WCRY G +D G A DP + Sbjct: 379 SNRQPKFIIPTIADRLKAGK----GVAGLALESALWCRYCFGTSDSG-AVIEPNDPSWDR 433 Query: 297 LQ 298 LQ Sbjct: 434 LQ 435 >YPO1280 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 490 Score = 107 bits (266), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 93/309 (30%), Positives = 146/309 (47%), Gaps = 35/309 (11%) Query: 18 PMTFVSMDNCSHNGDKLKKGVVTIAKEWTARGLVEEGFVAYLENEQLIAFPLSMIDKITP 77 P T +S DN NGD ++ V+ +A T R ++ +Q + FP +M+D+I P Sbjct: 179 PFTVLSCDNIPENGDVVRNAVLGLA---TVRDPALANWI-----QQHVTFPNTMVDRIVP 230 Query: 78 RP-SETVQAALLEQGIGGMDVIITSKNTYTAPFVNAEISEYLVIEDKFTNGRPLLEEAGI 136 +ET+Q G+ S PF+ V+EDKF GRP + AG+ Sbjct: 231 AATAETLQEIADTLGVA------DSCGIACEPFIQ------WVVEDKFVAGRPDWQVAGV 278 Query: 137 IFTDRDTVNNVETMKVTTCLNPLHTALAVSGCLLGYSLIADEMKDDTLRRFVEIIGYKEG 196 D V E MK+ LN H+ L+ G L GY I D M D+ R + E Sbjct: 279 QLV--DDVLPFEEMKLRM-LNGSHSYLSYLGYLAGYQHINDCMADENYRLTARRLMMNEQ 335 Query: 197 LPVVVDPGILSPKQFLDEVLTERFANPFIPDTPQRIATDTSQKVGIRFGETIKSYIRREE 256 P + GI + D+ L ER+ NP + +IA D SQK+ R ++++ ++++ Sbjct: 336 APTLRVTGI-DLNAYADQ-LIERYCNPALKHRTWQIAMDGSQKLPQRMLDSVRWHLKQGN 393 Query: 257 LDPAQLTAIPLAIAVWCRYLLGVNDEGEAFTLSPDPLLETLQGHLQGVALG-DRNASVRP 315 P + L IA W RY+ G++D G+ + DP++++ + Q VA D A V+ Sbjct: 394 AYP----CLALGIAGWMRYVGGIDDNGQVIDIR-DPMVDSFK---QCVAASEDGAARVQS 445 Query: 316 ILEDEQLFG 324 +L + LFG Sbjct: 446 LLTLKALFG 454 >SMc01501 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 494 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 84/282 (29%), Positives = 131/282 (46%), Gaps = 28/282 (9%) Query: 3 IVASLAYKRYLKGAFPMTFVSMDNCSHNGDKLKKGVVTIAKEWTARGLVEEGFVAYLENE 62 I+A LA +R KG P T +S DN NG+ V +A+ L + GF +++ Sbjct: 169 ILAGLAERR-AKGIPPFTIMSCDNIPGNGEVTHAAVSGLAR------LSDPGFADWIDAN 221 Query: 63 QLIAFPLSMIDKITPRPSETVQAALLEQGIGGMDVIITSKNTYTAPFVNAEISEYLVIED 122 +AFP M+D+ITP G + ++ + A V E + V+ED Sbjct: 222 --VAFPNGMVDRITP-----------ATGAREIGIVASQYGIDDAWPVFCEEFKQWVLED 268 Query: 123 KFTNGRPLLEEAGIIFTDRDTVNNVETMKVTTCLNPLHTALAVSGCLLGYSLIADEMKDD 182 +F GRP LEE G+ F V E MK+ LN H A+A LL + + M++ Sbjct: 269 RFPQGRPALEEVGVQFV--PDVAPYEHMKI-RILNGGHAAIAYPAALLDIHFVHEAMEEP 325 Query: 183 TLRRFVEIIGYKEGLPVVVDPGILSPKQFLDEVLTERFANPFIPDTPQRIATDTSQKVGI 242 +R F+ + + E +PV+ K + +++ RF+NP I DT R+A D S + Sbjct: 326 LIRAFLSKLEHDEIIPVIPPVPDTDLKDYY-KLIETRFSNPKIGDTVARLAQDGSNRQPK 384 Query: 243 RFGETIKSYIRREELDPAQLTAIPLAIAVWCRYLLGVNDEGE 284 + +RR E + + L A+WCRY G +D G+ Sbjct: 385 FILPSTADRLRRGE----DVVGLSLVSALWCRYFAGKSDSGK 422 >ZyeiQ [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 488 Score = 102 bits (255), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 95/320 (29%), Positives = 153/320 (47%), Gaps = 42/320 (13%) Query: 10 KRYLKGAFPMTFVSMDNCSHNGDKLKKGVVTIAKEWTARGLVEEGFVAYLENEQLIAFPL 69 +R +G P T +S DN NG +K V+ +A++ R G++ ++ ++FP Sbjct: 171 RRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEK---RSPELAGWI-----KEHVSFPG 222 Query: 70 SMIDKITPRPSETVQAALLE--QGIGGMDVIITSKNTYTAPFVNAEISEYLVIEDKFTNG 127 +M+D+I P ++ +L+E Q +G D S PF+ V+ED F G Sbjct: 223 TMVDRIVPAATDE---SLVEISQHLGVNDPCAIS----CEPFIQ------WVVEDNFVAG 269 Query: 128 RPLLEEAGIIFTDRDTVNNV---ETMKVTTCLNPLHTALAVSGCLLGYSLIADEMKDDTL 184 RP E AG+ VN+V E MK+ LN H+ LA G L G++ I+D M+D Sbjct: 270 RPAWEVAGV-----QMVNDVLPWEEMKLRM-LNGSHSFLAYLGYLSGFAHISDCMQDRAF 323 Query: 185 RRFVEIIGYKEGLPVVVDPGILSPKQFLDEVLTERFANPFIPDTPQRIATDTSQKVGIRF 244 R + E P + + Q+ D+++ RFANP + +IA D SQK+ R Sbjct: 324 RHAARTLMLDEQAPTLQIKDV-DLTQYADKLIA-RFANPALKHKTWQIAMDGSQKLPQRM 381 Query: 245 GETIKSYIRREELDPAQLTAIPLAIAVWCRYLLGVNDEGEAFTLSPDPLLETLQGHLQGV 304 I+ + RE + + L +A W RY+ GV+D G A + DPL + ++ + G Sbjct: 382 LAGIRIHQGRE----TDWSLLALGVAGWMRYVSGVDDAGNAIDVR-DPLSDKIRELVAGS 436 Query: 305 ALGDRNASVRPILEDEQLFG 324 + R V +L ++FG Sbjct: 437 SSEQR---VTALLSLREVFG 453 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,916,396 Number of extensions: 1199623 Number of successful extensions: 2432 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 2315 Number of HSP's successfully gapped: 35 Length of query: 359 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 251 Effective length of database: 25,922,359 Effective search space: 6506512109 Effective search space used: 6506512109 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)