BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0012.p (260 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll0231 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 99 6e-21 PA1415 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 92 6e-19 mll5024 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 78 2e-14 AF0575 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 70 3e-12 STM0997 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 69 6e-12 >mll0231 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 253 Score = 99.0 bits (245), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 64/184 (34%), Positives = 95/184 (51%), Gaps = 4/184 (2%) Query: 35 GQERAALIDTGLGIGNIRTITDQLTNLPIDVLTTHVHTDHIGSHGEYERIFVHEGDLDWL 94 G++R L DTGLG ++RT +T + + +H H DHIG H E+ VH + L Sbjct: 43 GRDRDLLFDTGLGHFSLRTHVPLVTERKLTCVASHTHFDHIGCHHEFPDRGVHPAEAKIL 102 Query: 95 VHGIKGLSLEQIRRDIGRDISQPVPEGFDPQTYRPFQGEPAGLLRDGDTVELGGRALAVY 154 ++ R D+ +P+G++ YR LL GD V+LG RA V Sbjct: 103 ADPRNEWTVAD--RYATDDMFDGLPQGWESSRYRILPAPADRLLDHGDVVDLGDRAFEVI 160 Query: 155 HTPGHSPGHLVFWDTLNGYLFTGDLLYDGTPVYAFYPSTSPVDLVASLERIAEIDGVQRI 214 HTPGHSPG + ++ G L +GD++YDG + Y S P D +A+L + E+D V + Sbjct: 161 HTPGHSPGGIALYEKKTGILLSGDIIYDGPLIDDVYHSDIP-DYMATLLAMRELD-VSVV 218 Query: 215 YGSH 218 +G H Sbjct: 219 HGGH 222 >PA1415 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 242 Score = 92.4 bits (228), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 63/195 (32%), Positives = 101/195 (51%), Gaps = 9/195 (4%) Query: 27 KVHSFLLLGQERAALIDTGLGIGNIRTITDQLTNLPIDVLTTHVHTDHIGSHGEYERIFV 86 + + + + G+ER L+D+G G+ ++ LT P+ + +H H DHI H E+ Sbjct: 32 RCNMWHVQGRERDVLVDSGSGLVSLCEQLPWLTERPLLAVASHTHFDHIAGHHEFAERLA 91 Query: 87 HEGDLDWLVHGIKGLSLEQIRRDIGRDISQPVPEGFDPQTYRPFQ--GEPAG-LLRDGDT 143 H + + L +L R +G ++ + PE P Y ++ PA L+ +GD Sbjct: 92 HPAEAEILAAPDGDNTL--ARAYVGDEMFEAHPEC--PLCYAEYRVRAAPATRLIDEGDV 147 Query: 144 VELGGRALAVYHTPGHSPGHLVFWDTLNGYLFTGDLLYDGTPVYAFYPSTSPVDLVASLE 203 ++LG R L V HTPGHSPG + W+ LF+GD++YDG V Y S D +SL Sbjct: 148 LDLGDRVLQVLHTPGHSPGGISLWEAATQTLFSGDIVYDGPLVEDAYHSNLD-DYASSLA 206 Query: 204 RIAEIDGVQRIYGSH 218 R+ E+ V+ ++G H Sbjct: 207 RLRELP-VRTVHGGH 220 >mll5024 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 238 Score = 77.8 bits (190), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 66/252 (26%), Positives = 117/252 (46%), Gaps = 33/252 (13%) Query: 6 WFTVQQIDETTFAISE-YGH-WEKVHSFLLLGQERAALIDTGLGIGNIRTITDQLTNLPI 63 WF+ +D+ T ++E + H + + + + L G++ L+DTG+GI + + P+ Sbjct: 3 WFSKSIVDDRTTMLTEPFVHDYVRANIWHLRGRDADLLVDTGMGICPLAPQIETPRGKPL 62 Query: 64 DVLTTHVHTDHIGSHGEYE-----RIFVHE-------GDLDWLVHGIKGLSLEQIRRDIG 111 V+ TH+H DH+GS E+ R+ + W+ H ++G Sbjct: 63 LVVATHIHLDHVGSLHEFSWRAGPRMSAAQFESMDEAATYAWMFHDLEGA---------- 112 Query: 112 RDISQPVPEGFDPQTYRPFQGEPAGLLRDGDTVELGGRALAVYHTPGHSPGHLVFWDTLN 171 +S+ G+ Y+ L +GD ++LG R V H PGHSP + +D + Sbjct: 113 --VSKLPAPGWRSADYKIPSAPLTRTLDEGDVIDLGDRQFRVLHLPGHSPDSIALFDEAD 170 Query: 172 GYLFTGDLLYDGTPVYAFYPSTSPVDLVASLERIAEIDGVQRI-YGSHNTLGLHPQILSE 230 G F+GD +YD T + + S V+++ R+ +D + RI +G H + + E Sbjct: 171 GLFFSGDAIYDDTLIDSLQDSDRAA-YVSTMRRL--LDLLIRIGHGGHGP-SFDGKRMRE 226 Query: 231 VKHAVQLLRRND 242 + A LRR+D Sbjct: 227 I--ATDYLRRHD 236 >AF0575 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 209 Score = 70.5 bits (171), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 61/205 (29%), Positives = 101/205 (49%), Gaps = 39/205 (19%) Query: 28 VHSFLLLGQERAALIDTGLGIGNIRTITDQLTNLPID------VLTTHVHTDHIGSHGEY 81 + +LLL E+ ALID G G+I+ I + L ID ++ TH H DH + G + Sbjct: 13 ANCYLLL-DEKKALIDVG---GDIQFILNALKRY-IDPRELDYIILTHSHYDHAAAAGHF 67 Query: 82 ERIFVHEGDLDWLVHGIKGLSLEQIRRDIGRDISQPVPEGFDP----QTYRPFQGEPAGL 137 KG++ I +D + S +GF +++ F EP + Sbjct: 68 -----------------KGIAKIAIHKD---EYSLLKAQGFTSFMFGVSFKKF--EPDIM 105 Query: 138 LRDGDTVELGGRALAVYHTPGHSPGHLVFWDTLNGYLFTGDLLYD-GTPVYAFYPSTSPV 196 L G+ +ELG +L V HTPGHSPG + ++ +LF+GD ++ G+ P S Sbjct: 106 LEGGEVIELGELSLEVIHTPGHSPGSICLYEPERKWLFSGDTVFAHGSFGRVDLPGGSAR 165 Query: 197 DLVASLERIAEIDGVQRIYGSHNTL 221 +L+ SLE+++++D V+ +Y H + Sbjct: 166 ELINSLEKLSKLD-VENLYPGHEDI 189 >STM0997 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 215 Score = 68.9 bits (167), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 52/181 (28%), Positives = 82/181 (45%), Gaps = 25/181 (13%) Query: 30 SFLLLGQER-AALIDTGLGIGNIRTITDQLTNLPIDVLTTHVHTDHIGSHGEYER----- 83 S + Q R AAL+D G I+ D + +L TH H DH+G+ E + Sbjct: 16 SLIWCEQTRLAALVDPGGDAEKIKQEVDASGVTLMQILLTHGHLDHVGAASELAQHYGVP 75 Query: 84 IFVHEGDLDWLVHGIKGLSLEQIRRDIGRDISQPVPEGFDPQTYRPFQGEPAGLLRDGDT 143 + E + ++ + G+ S R G D QP+ P L DGD Sbjct: 76 VIGPEKEDEFWLQGLPAQS-----RMFGLDECQPL--------------TPDRWLNDGDR 116 Query: 144 VELGGRALAVYHTPGHSPGHLVFWDTLNGYLFTGDLLYDGTPVYAFYPSTSPVDLVASLE 203 V +G L V H PGH+PGH+VF+D + L +GD+++ G + +P L+ +++ Sbjct: 117 VSVGNVTLQVLHCPGHTPGHVVFFDEQSQLLISGDVIFKGGVGRSDFPRGDHTQLIDAIK 176 Query: 204 R 204 R Sbjct: 177 R 177 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.142 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,802,699 Number of extensions: 1020350 Number of successful extensions: 2000 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 2006 Number of HSP's successfully gapped: 46 Length of query: 260 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 156 Effective length of database: 26,383,695 Effective search space: 4115856420 Effective search space used: 4115856420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)