BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0001.p (241 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG0470G [V] COG1131 ABC-type multidrug transport system, ATPase... 120 2e-27 Ta0233 [V] COG1131 ABC-type multidrug transport system, ATPase c... 118 9e-27 MA0853 [V] COG1131 ABC-type multidrug transport system, ATPase c... 115 5e-26 TVN0149 [V] COG1131 ABC-type multidrug transport system, ATPase ... 114 9e-26 Ta0066 [V] COG1131 ABC-type multidrug transport system, ATPase c... 114 1e-25 >VNG0470G [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 252 Score = 120 bits (301), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 59/157 (37%), Positives = 95/157 (60%), Gaps = 2/157 (1%) Query: 3 ALKLEQVMKQYGDKTAVNGISLKVEPGEIYGLLGANGAGKTTTMRMVLGLIYPDGGKILY 62 A++ + + K+YGD+ AV+ + L V G +YG LG NGAGKTTTMR++ L P G Sbjct: 5 AIRADGLTKRYGDEAAVDDLDLAVPAGSVYGFLGPNGAGKTTTMRLLTSLTDPTAGTAEV 64 Query: 63 NGKPFSDE--LQHMMGYLPEERGLYPKVKVSDQIIYLARLRGMSAGDAEKSLRFWLERFD 120 G P +D L +GYLP + ++ ++ +Q+ ++ARL G+ G+A+ + LERFD Sbjct: 65 AGVPVTDRAALTPRIGYLPADPPVFDELTGWEQLRHVARLHGLDDGEADTRIESLLERFD 124 Query: 121 VPEYYNKKIEELSKGNQQKMGFIAAVVHKPQILILQH 157 + +++IE S G +K+G +AA++H P +L L Sbjct: 125 LLADADRRIESYSTGMTKKVGVVAAMLHDPDVLFLDE 161 >Ta0233 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 239 Score = 118 bits (295), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 59/161 (36%), Positives = 95/161 (59%), Gaps = 3/161 (1%) Query: 1 MEALKLEQVMKQYGDKTAVNGISLKVEPGEIYGLLGANGAGKTTTMRMVLGLIYPDGGKI 60 M ++ V K +G K A++G+S VE E L+G NGAGK+TT++++ GLI PD G + Sbjct: 4 MNCIEFNDVTKSFGTKKALDGLSFDVECQESVALIGPNGAGKSTTLKILAGLIKPDSGSV 63 Query: 61 LYNG-KPFSDELQHMMGYLPEERGLYPKVKVSDQIIYLARLRGMSAGDAEKSLRFWLERF 119 G P S E + +MGYLPE+ Y + V + + Y+ LR D E+ + F L Sbjct: 64 KVGGFDPDSIEAKRLMGYLPEDASPYLTLSVKENLEYIGALR--KTEDLEQKVEFLLNLM 121 Query: 120 DVPEYYNKKIEELSKGNQQKMGFIAAVVHKPQILILQHAVD 160 ++ EY N ++ LS+GN+QK+ A++H P+I++L ++ Sbjct: 122 ELNEYRNSRVSTLSRGNRQKLSIALAIIHDPKIMLLDEPLN 162 >MA0853 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 314 Score = 115 bits (289), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 69/172 (40%), Positives = 102/172 (59%), Gaps = 10/172 (5%) Query: 2 EALKLEQVMKQYGDKTAVNGISLKVEPGEIYGLLGANGAGKTTTMRMVLGLIYPDGGKIL 61 E +K +++ K +GD AVN ISL V GEIYG LG +GAGKTTT+RM+LG+I P G Sbjct: 10 EVIKTDRLSKDFGDLHAVNSISLCVGKGEIYGFLGLSGAGKTTTIRMLLGMIKPTSGAAY 69 Query: 62 YNGKPFS---DELQHMMGYLPEERGLYPKVKVSDQIIYLARLRGMSAGDAEKSLRFWLER 118 NGK + EL +GYL E YP + V +++ + RLRG+ D ++ + + Sbjct: 70 LNGKLVNAGETELWKNVGYLVELPYSYPDLTVKEKLEIIRRLRGI---DDTNAISAVMGK 126 Query: 119 FDVPEYYNKKIEELSKGNQQKMGFIAAVVHKPQILILQHAVDRPVAADDDGG 170 + +Y ++K +LS+GN+Q++G A++H P+ILIL D P D G Sbjct: 127 LQLNKYADRKAGKLSQGNRQRLGIAKALIHSPEILIL----DEPSNGLDPAG 174 >TVN0149 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 239 Score = 114 bits (286), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 54/161 (33%), Positives = 99/161 (61%), Gaps = 3/161 (1%) Query: 1 MEALKLEQVMKQYGDKTAVNGISLKVEPGEIYGLLGANGAGKTTTMRMVLGLIYPDGGKI 60 ME + EQV K YG K A++G++ +++ GE L+G NGAGK+TT++++ G++ PD G + Sbjct: 1 MECIHFEQVSKYYGKKRAIDGLTFRLDCGESMALIGPNGAGKSTTLKILAGILKPDSGSV 60 Query: 61 LYNG-KPFSDELQHMMGYLPEERGLYPKVKVSDQIIYLARLRGMSAGDAEKSLRFWLERF 119 +G P +++ + ++GYLPE+ Y + V + + Y+ LRG+ + + F +E Sbjct: 61 SISGYDPSTNDARKIVGYLPEDAAPYFTLSVRENLEYIGALRGVQ--NLNSRMDFLMELL 118 Query: 120 DVPEYYNKKIEELSKGNQQKMGFIAAVVHKPQILILQHAVD 160 + Y N K+ LS+GN+QK+ ++H P++L+L ++ Sbjct: 119 GLGNYRNVKVSALSRGNRQKLAIALVLIHDPKVLLLDEPLN 159 >Ta0066 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 260 Score = 114 bits (286), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 56/161 (34%), Positives = 99/161 (61%), Gaps = 3/161 (1%) Query: 3 ALKLEQVMKQYGDKTAVNGISLKVEPGEIYGLLGANGAGKTTTMRMVLGLIYPDGGKIL- 61 ++K++ V +YG+K A++G++L + G IYG+LG NG+GKTT MR++LGL+ G + Sbjct: 5 SIKIDNVSMRYGEKLALDGVTLDIPEGTIYGILGPNGSGKTTLMRIILGLLKQTAGSAIV 64 Query: 62 --YNGKPFSDELQHMMGYLPEERGLYPKVKVSDQIIYLARLRGMSAGDAEKSLRFWLERF 119 Y+ +++ + GY+PE LY + S+ ++A +R M G + + ++ F Sbjct: 65 CGYDSTRDQMKIKEISGYVPETPILYESMTPSELFSFIASIRRMDEGTFRRRVDALIDAF 124 Query: 120 DVPEYYNKKIEELSKGNQQKMGFIAAVVHKPQILILQHAVD 160 + EY N+ I LS GN+QK+ I+A++H P+ILI+ ++ Sbjct: 125 GIAEYINEFIGSLSFGNRQKVAIISAILHDPKILIVDEGIN 165 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,403,335 Number of extensions: 808881 Number of successful extensions: 5410 Number of sequences better than 1.0e-05: 1568 Number of HSP's gapped: 4641 Number of HSP's successfully gapped: 1634 Length of query: 241 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 138 Effective length of database: 26,499,029 Effective search space: 3656866002 Effective search space used: 3656866002 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)