BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0220.0002.p (344 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20117 [M] COG0438 Glycosyltransferase 150 3e-36 mll0693 [M] COG0438 Glycosyltransferase 135 8e-32 CAC1053 [M] COG0438 Glycosyltransferase 62 2e-09 AF0606 [M] COG0438 Glycosyltransferase 60 5e-09 BH1415_2 [M] COG0438 Glycosyltransferase 59 9e-09 >SMb20117 [M] COG0438 Glycosyltransferase Length = 366 Score = 150 bits (379), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 110/332 (33%), Positives = 167/332 (50%), Gaps = 45/332 (13%) Query: 29 PSIKGGTEKIVYELTEELVRRGHKVYLFAALGSRSRAKLITYPK-GLR-----HESIARF 82 P + GGTE++V+ LTEELVR+GH+V LFA+ S + AKLI LR + I Sbjct: 15 PKLYGGTERVVHHLTEELVRQGHEVTLFASGDSMTSAKLIPCSDMALRLNPAVQDPIPYH 74 Query: 83 VL------------KKLPERVDIIHDHTFRSALGVTNPKVPTVCTVHIPVKRRIKYPVY- 129 +L L +D++H RS G TV T+H + P Y Sbjct: 75 MLMLEEVRRQANGFDVLHFHIDLLHFPLVRSLAG------KTVTTLHGRLDLPDLQPFYA 128 Query: 130 ---------VSKRARRLMGN-NKGFCVYNGINTREYEFSTE-KHLYLLYIGRIIRNKGVM 178 +S R+ M N V++G+ F+ + + YL ++GRI K Sbjct: 129 AFPDVPLISISDDQRKPMPPVNWVATVHHGLAPDVLPFTGQPRGDYLAFLGRISPEKRPD 188 Query: 179 QAIQVAEKTGKRLIIAGPVKASR--FFEREVKPRIRNNPNIQYVGPVGGKMKQQLLKHAE 236 +AI++A + G L IA V + ++ E++P +R PN++++G + K + L +A Sbjct: 189 RAIEIAARVGMPLKIAAKVDKADEAYWLNEIEPLVRRYPNVEFIGEIDECQKGEFLGNAR 248 Query: 237 CVLFPTLWEEPFGLVLIESLGCGTPVLALRNGAVPEIL----SGFPNLICNNISEMAVKV 292 +LFP W EPFGLV+IE++ CGTPV+A R GAVPE++ SGF I +++ E V Sbjct: 249 ALLFPIDWPEPFGLVMIEAMACGTPVVAFRCGAVPEVIDHAVSGF---IVDSMEEAVKAV 305 Query: 293 RQKRYPSPHLLRAYVQKHFTNKQMTDSYLRIY 324 H +RA + FT ++M D YL +Y Sbjct: 306 HDLDRLDRHTVRATFDRRFTARRMADDYLDLY 337 >mll0693 [M] COG0438 Glycosyltransferase Length = 301 Score = 135 bits (341), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 0/327 (0%), Positives = 0/327 (0%), Gaps = 32/327 (9%) Query: 29 PSIKGGTEKIVYELTEELVRRGHKVYLFAALGSRSRAKLI--------TYPKGLRHESIA 80 G ++ +L + + + T + + Sbjct: 15 GRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLHMEEKRAI 74 Query: 81 RFVL----KKLPERVDIIHDHT--------FRSA--LGVTNPKVP-TVCTVHIPVKRRIK 125 ++ V+++ V+ + Sbjct: 75 NRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKAD 134 Query: 126 YP-VYVSKRAR-RLMGNNKGFCVYNGINTREYEFSTEKHL---YLLYIGRIIRNKGVMQA 180 ++ + + + I A Sbjct: 135 LLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMA 194 Query: 181 IQVAEKTGKRLIIAGPVKASR--FFEREVKPRIRNNPNIQYVGPVGGKMKQQLLKHAECV 238 ++ + R + A + R + K+ ++ + Sbjct: 195 SEIIREKLMRFLGAELPYSVTVEIERFVSNERG-GYDINGLILVEREGQKKMVIGNKGAK 253 Query: 239 LFPTLWEEPFGLVLIESLGCGTPVLALRNGAVPEILS-GFPNLICNNISEMAVKVRQKRY 297 + E + + + + Sbjct: 254 IKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERA 313 Query: 298 PSPHLLRAYVQKHFTNKQMTDSYLRIY 324 Sbjct: 314 340 >CAC1053 [M] COG0438 Glycosyltransferase Length = 466 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 72/315 (22%), Positives = 120/315 (38%), Gaps = 71/315 (22%) Query: 29 PSIKGGTEKIVYELTEELVRRGHKVYLF------AALGSRSRAKLI--TYPKGLRHESIA 80 P GG VY L+ L GH+VY+ A + + P + E Sbjct: 12 PKNVGGLSNHVYNLSHALASLGHEVYVVTCEEKTAPVEENDDGVYVHRVTPYKIDTEDFT 71 Query: 81 RFV-------------LKKLPERVDIIHDHTFRSA----LGVTNPKVPTVCTVHIPVKRR 123 ++V L K +VD+IH H + + + K+P VCT+H K R Sbjct: 72 KWVMHLNFSMIEECTRLMKKIGKVDMIHVHDWLCVYCGKVLKWSYKIPMVCTIHATEKGR 131 Query: 124 -----IKYPVYVSKRARRLMGNN-----------------------KGFCVYNGINTREY 155 + Y+S L + K + + NGI+ + Sbjct: 132 NNGIRTEMQRYISSAEWLLTYESWKIVACSGYMKAQIVDTFNTPEEKVWIIPNGIDLNSF 191 Query: 156 EF-----------STEKHLYLLYIGRIIRNKGVMQAIQ-----VAEKTGKRLIIAGPVKA 199 +F + + + +IGR + KG+ I V+E + IIAG Sbjct: 192 DFDFDWLKFRRKYACDDEKIVFFIGRHVFEKGIQILIDAAPGIVSEYNKTKFIIAGTGPM 251 Query: 200 SRFFEREVKPRIRNNPNIQYVGPVGGKMKQQLLKHAECVLFPTLWEEPFGLVLIESLGCG 259 + + +VK I + G + K K++ + A +FP+L+E PFG+VL+E++ G Sbjct: 252 TEELKDKVKS-IGLQDKFLFTGYMDNKTKKKFYRVASVAVFPSLYE-PFGIVLLEAMAAG 309 Query: 260 TPVLALRNGAVPEIL 274 P + G EI+ Sbjct: 310 CPAVVSDTGGFGEII 324 >AF0606 [M] COG0438 Glycosyltransferase Length = 363 Score = 60.1 bits (144), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 43/169 (25%), Positives = 87/169 (51%), Gaps = 12/169 (7%) Query: 146 VYNGINTREYEFSTEKHLYLLYIGRIIRNKGVMQAIQVAEKTGKRLIIAGPVKASRFFER 205 +Y I +Y+ + YL ++GR+ K +AI+ K K++++ G S + E+ Sbjct: 176 LYPPIEFDKYKCKNSEDFYL-FVGRLWHEKRPEEAIRGCIKAKKKIVVIG----SGYLEK 230 Query: 206 EVKPRIRNNPNIQYVGPVGGKMKQQLLKHAECVLFPTLWEEPFGLVLIESLGCGTPVLAL 265 ++ + NP ++ G V + K LL + V++P + E FG+V IE+ G PV+A Sbjct: 231 YLRDKYGKNPYVEIKGFVSEEEKVDLLASCKAVIYPCI-AEDFGIVPIEAFASGKPVIAD 289 Query: 266 RNGAVPEILSGFPNLIC-----NNISEMAVKVRQKRYPSPHLLRAYVQK 309 +G P +++ +I +NI++ ++ +K+Y P +R + ++ Sbjct: 290 NSGFPPYVVNEERGIITDCSNPDNIAKAIEELEKKKY-DPDKIREFAKQ 337 >BH1415_2 [M] COG0438 Glycosyltransferase Length = 385 Score = 59.3 bits (142), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 39/150 (26%), Positives = 71/150 (47%), Gaps = 13/150 (8%) Query: 141 NKGFCVYNGINTREYE------FSTEKHLYLLYIGRIIRNKGVMQAIQVAEKTGK----- 189 +K + NG+ + E S E + +GRI++ KG I+ A K + Sbjct: 174 DKVAVIANGVAREQIEAARLQTISPENRFIVFSVGRIVQEKGFSLLIEAAAKCKELGEPI 233 Query: 190 RLIIAGPVKASRFFEREVKPRIRNNPNIQYVGPVGGKMKQQLLKHAECVLFPTLWEEPFG 249 + ++AG ++++VK R I +VG + + + A+ +FP+L+E PFG Sbjct: 234 QFVVAGHGPLLADYQQQVKER-HLEAWISFVGYISDSERNEWYHRADVCIFPSLYE-PFG 291 Query: 250 LVLIESLGCGTPVLALRNGAVPEILSGFPN 279 +V +E++ GTP + G + EI+ N Sbjct: 292 IVALEAMAAGTPTIVSDTGGLAEIVEHGDN 321 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,447,072 Number of extensions: 1085909 Number of successful extensions: 2013 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2039 Number of HSP's successfully gapped: 15 Length of query: 344 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 237 Effective length of database: 26,037,693 Effective search space: 6170933241 Effective search space used: 6170933241 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)