BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0025.p (255 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0711 [K] COG2207 AraC-type DNA-binding domain-containing proteins 195 5e-50 STM0104 [K] COG2207 AraC-type DNA-binding domain-containing prot... 70 3e-12 araC [K] COG2207 AraC-type DNA-binding domain-containing proteins 68 1e-11 ECs0068 [K] COG2207 AraC-type DNA-binding domain-containing prot... 68 1e-11 lin2267 [K] COG2207 AraC-type DNA-binding domain-containing prot... 67 4e-11 >BH0711 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 263 Score = 195 bits (496), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 91/240 (37%), Positives = 147/240 (61%), Gaps = 9/240 (3%) Query: 10 YLLRLQTEGRSRTMVNGVLTTVESGDLMLFAPSDPYYLSIDKESYPVGKPRIESGDYHIF 69 YLLRLQTEG + +N +E GDL+L P D + L ++ SGDYH+ Sbjct: 26 YLLRLQTEGTCKVKLNEREFQLEKGDLLLTNPEDMFELIVEDGD--------NSGDYHLL 77 Query: 70 CGGPWIEEWWTRKQRPLVLPMPLSDYFLGLFRQIVLEQRR-LSNPSPEISACYLQILCLE 128 C G WI+ WW R ++P V + L + + ++R ++LE RR +S + EI +Q LCL Sbjct: 78 CEGDWIDAWWRRSEKPSVSTIDLDENIMAIWRCMILEDRRSVSEKNDEILHYLVQTLCLS 137 Query: 129 IDRLMSEQPAVSPKGYLAYRMKQYVEEHAAYPFRLEDVAGHVDISVSRAVHLFKEAFGTT 188 ++R E ++S + Y RM +Y+EE+A ++EDVA H +SVSR+ HLFK + G T Sbjct: 138 LERAAMETKSMSSRPYTVTRMMRYIEENAITGIKVEDVAKHAGLSVSRSAHLFKSSVGKT 197 Query: 189 IVKYVNDVRLDMARERITFSPMPLEHVSETCGFANYTYFHRLFRSRFGMSPKQYRAYSRQ 248 +++Y ++++L +A + + + LEH++E CGF +Y+YFHR+F+ +G+SP YR+ ++ Sbjct: 198 MIEYAHEIKLAVAIDFLKHTTYTLEHIAEDCGFGSYSYFHRVFKKHYGISPGDYRSSTKH 257 >STM0104 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 281 Score = 70.1 bits (170), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 66/247 (26%), Positives = 105/247 (42%), Gaps = 25/247 (10%) Query: 10 YLLRLQTEGRSRTMVNGVLTTVESGDLMLFAPSDPYYLSIDKESYPVGKPRIESGDYH-- 67 Y+L L G NG GD++LF P + ++ G+ S YH Sbjct: 45 YILNLTIRGEGVINNNGEQFVCRPGDILLFPPGEIHH---------YGRHPDASEWYHQW 95 Query: 68 -IFCGGPWIEEWWTRKQ--------RPLVLPMPLSDYFLGLFRQIVLEQRRLSNPSPEIS 118 F + +EW T RP P +F LF QI+ + S ++ Sbjct: 96 VYFRPRAYWQEWLTWPTIFAQTGFFRPDEARQP---HFSELFGQIISAGQGEGRYSELLA 152 Query: 119 ACYLQILCLEIDRLMSEQPAVSPKGYLAYRMKQYVEEHAAYP-FRLEDVAGHVDISVSRA 177 L+ L L +++E P QY+ +H A F + VA HV +S SR Sbjct: 153 INLLEQLLLRRMAVINES-LHPPMDSRVRDACQYISDHLADSHFDIASVAQHVCLSPSRL 211 Query: 178 VHLFKEAFGTTIVKYVNDVRLDMARERITFSPMPLEHVSETCGFANYTYFHRLFRSRFGM 237 HLF++ G +++ + D R+ A+ ++ + MP+ V GF + YF R+F+ G Sbjct: 212 SHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGA 271 Query: 238 SPKQYRA 244 SP ++RA Sbjct: 272 SPSEFRA 278 >araC [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 292 Score = 68.2 bits (165), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 65/245 (26%), Positives = 111/245 (45%), Gaps = 21/245 (8%) Query: 10 YLLRLQTEGRSRTMVNGVLTTVESGDLMLFAPSDPYYLSIDKES------YPVGKPRIES 63 Y+L L G+ G GD++LF P + ++ E+ + +PR Sbjct: 45 YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPR--- 101 Query: 64 GDYHIFCGGPWIEEWWTRKQRPLVLPMPLSDYFLGLFRQIVLEQRRLSNPSPEISACYLQ 123 +H + P I T RP P +F LF QI+ + S ++ L+ Sbjct: 102 AYWHEWLNWPSIFAN-TGFFRPDEAHQP---HFSDLFGQIINAGQGEGRYSELLAINLLE 157 Query: 124 ILCLEIDRLMSEQPAVSPKGYLAYRMK---QYVEEH-AAYPFRLEDVAGHVDISVSRAVH 179 L L R+ + ++ P + R++ QY+ +H A F + VA HV +S SR H Sbjct: 158 QLLLR--RMEAINESLHPP--MDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSH 213 Query: 180 LFKEAFGTTIVKYVNDVRLDMARERITFSPMPLEHVSETCGFANYTYFHRLFRSRFGMSP 239 LF++ G +++ + D R+ A+ ++ + MP+ V GF + YF R+F+ G SP Sbjct: 214 LFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASP 273 Query: 240 KQYRA 244 ++RA Sbjct: 274 SEFRA 278 >ECs0068 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 292 Score = 68.2 bits (165), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 65/245 (26%), Positives = 111/245 (45%), Gaps = 21/245 (8%) Query: 10 YLLRLQTEGRSRTMVNGVLTTVESGDLMLFAPSDPYYLSIDKES------YPVGKPRIES 63 Y+L L G+ G GD++LF P + ++ E+ + +PR Sbjct: 45 YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPR--- 101 Query: 64 GDYHIFCGGPWIEEWWTRKQRPLVLPMPLSDYFLGLFRQIVLEQRRLSNPSPEISACYLQ 123 +H + P I T RP P +F LF QI+ + S ++ L+ Sbjct: 102 AYWHEWLNWPSIFAN-TGFFRPDEAHQP---HFSDLFGQIINAGQGEGRYSELLAINLLE 157 Query: 124 ILCLEIDRLMSEQPAVSPKGYLAYRMK---QYVEEH-AAYPFRLEDVAGHVDISVSRAVH 179 L L R+ + ++ P + R++ QY+ +H A F + VA HV +S SR H Sbjct: 158 QLLLR--RMEAINESLHPP--MDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSH 213 Query: 180 LFKEAFGTTIVKYVNDVRLDMARERITFSPMPLEHVSETCGFANYTYFHRLFRSRFGMSP 239 LF++ G +++ + D R+ A+ ++ + MP+ V GF + YF R+F+ G SP Sbjct: 214 LFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASP 273 Query: 240 KQYRA 244 ++RA Sbjct: 274 SEFRA 278 >lin2267 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 292 Score = 66.6 bits (161), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 55/222 (24%), Positives = 84/222 (37%), Gaps = 39/222 (17%) Query: 61 IESGDYHIFCGGPWIEEWWTRKQRPLVLPMPLSDYFLGLFRQIVLEQRRLSNPSPEISAC 120 I GD H F P E + L D G R+++ E LS PE + Sbjct: 64 INGGDVHYFLASPNSERIVIQFDLSLFQEEMQMDGQNGTLREMLTEMAHLSREWPEETTA 123 Query: 121 YLQILCLEI-------------------------------------DRLMSEQPAVSPKG 143 +Q L I D ++SE+ + K Sbjct: 124 KVQSLIRNIHLESSNDSPGKHYILKADLLAIIGLIYREIPQKKTQPDSVISEKAVLKSKE 183 Query: 144 YLAY--RMKQYVEEHAAYPFRLEDVAGHVDISVSRAVHLFKEAFGTTIVKYVNDVRLDMA 201 L ++ YVE+H P L++VA + S FK G T V ++ND RL+ A Sbjct: 184 TLQKLDQIFSYVEKHYQEPVSLQEVADYTGFSTYYFTKFFKRNTGMTFVTFLNDYRLNKA 243 Query: 202 RERITFSPMPLEHVSETCGFANYTYFHRLFRSRFGMSPKQYR 243 + + P+ V+E GF++ FH F+ G++P +YR Sbjct: 244 KWMLLNEAFPVTEVAELAGFSSVKTFHHAFKRAMGVAPLKYR 285 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,444,448 Number of extensions: 758255 Number of successful extensions: 2054 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 2060 Number of HSP's successfully gapped: 72 Length of query: 255 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 151 Effective length of database: 26,383,695 Effective search space: 3983937945 Effective search space used: 3983937945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)