BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0024.p (255 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2265 [G] COG1082 Sugar phosphate isomerases/epimerases 147 2e-35 BH3346 [G] COG1082 Sugar phosphate isomerases/epimerases 122 4e-28 mll3313 [G] COG1082 Sugar phosphate isomerases/epimerases 99 8e-21 CC1629 [G] COG1082 Sugar phosphate isomerases/epimerases 86 5e-17 AGl2948 [G] COG1082 Sugar phosphate isomerases/epimerases 86 5e-17 >lin2265 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 301 Score = 147 bits (370), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 11/242 (4%), Positives = 33/242 (13%), Gaps = 7/242 (2%) Query: 3 KIGLQLYTLREELEQDFEGTIRKVAELGYAGVEFFHYFGRTAEQVNALLQETGLTALGAH 62 + + T+ + V + Sbjct: 4 DKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDT 63 Query: 63 RPYDAMLNDTEQEISFNLSIGNRNLIVPYLT-EEQRNWEEVAVNLRKIGEQCSARGAVLS 121 + + + + + E W + + A + Sbjct: 64 PGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAV 123 Query: 122 YHNHDFEFKEQFGGRTAFDGIFEEVPADLLQVEMDTCWVYYAGYDPVEYIHKYAGRLPII 181 + + + + V + Sbjct: 124 NKVDNVQE-KADLLPHLQFLASQMNFL--DIVPISAETGLNVDTIAAIVRKHLPEATHHF 180 Query: 182 HLKDMKKREDGSAETVVLGEGEVKLXXXXXXXXXXXXXXXXXXQDFCSRSPLESVADSMK 241 + R S + KL R + + Sbjct: 181 PEDYITDR---SQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILV 237 Query: 242 WI 243 Sbjct: 238 ER 239 >BH3346 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 241 Score = 122 bits (307), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 67/188 (35%), Positives = 110/188 (58%), Gaps = 12/188 (6%) Query: 3 KIGLQLYTLREELEQDFEGTIRKVAELGYAGVEFF-HYFGRTAEQVNALLQETGLTALGA 61 +I LQLYT+R+ LE+DFEGT+RKVA +G+ GVE + G++ E + A+LQ GL + + Sbjct: 4 QIALQLYTVRKPLEEDFEGTLRKVASIGFQGVELADDWGGKSPEALKAILQSVGLVPVSS 63 Query: 62 HRPYDAMLNDTEQEISFNLSIGNRNLIVPYLTEEQRN---WEEVAVNLRKIGEQCSARGA 118 H + + N + I +L +G + PYL EE++ ++E+ +L++I ++ +G Sbjct: 64 HVALERLENQLDDVIDQHLRLGCHTIYCPYLEEERQTATGYKELITSLKEIQKKLEKKGI 123 Query: 119 VLSYHNHDFEFKEQFGGRTAFDGIFEEVPADLLQVEMDTCWVYYAGYDPVEYIHKYAGRL 178 YH+HDFE + I +P+ L E+D W+ AG DPVE++ +Y ++ Sbjct: 124 SFGYHHHDFELY-----SNQLEAIL-SIPS--LLAELDVYWLQKAGQDPVEWVSRYKHKV 175 Query: 179 PIIHLKDM 186 +HLKDM Sbjct: 176 RHVHLKDM 183 >mll3313 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 301 Score = 98.6 bits (244), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 12/196 (6%), Positives = 34/196 (17%), Gaps = 9/196 (4%) Query: 2 LKIGLQLYTLREELEQDFEGTIRKVAELGYAGVEFFHYFGRTAEQVNALLQETGLTALGA 61 + I + G + + + ++ A A Sbjct: 1 MSIDKSYCGFIAI--VGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQA 58 Query: 62 HRPYDAMLNDTEQEISFNLSIGNRNLIVPYLTEEQR-----NWEEVAVNLRKIGEQCSAR 116 L+ E+ L + + + W + + A Sbjct: 59 IYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAP 118 Query: 117 GAVLSYHNHDFEFKEQFGGRTAFDGIFEEVPADLLQVEMDTCWVYYAGYDPVEYIHKYAG 176 + + + K F + + + H Sbjct: 119 VILAVNKVDNVQEKADLLPHLQFLASQMNFLD--IVPISAETGLNVDTIAAIVRKHLPEA 176 Query: 177 RLPIIHLKDMKKREDG 192 + + Sbjct: 177 THHFPEDYITDRSQRF 192 >CC1629 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 328 Score = 86.3 bits (212), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 62/210 (29%), Positives = 97/210 (46%), Gaps = 29/210 (13%) Query: 4 IGLQLYTLREELEQDFEGTIRKVAELGYAGVEFFHYFGRTAEQVNALLQETGLTALGAH- 62 +G+QLY+L +L ++ + + VA++G+ VE Y GRT ++ AL GL AH Sbjct: 56 VGIQLYSLGPDLAKELDAQLATVAKIGFRTVELAGYLGRTPAELRALFDRHGLVCPSAHI 115 Query: 63 --RPYDAMLNDTEQEISFNLSIGNRNLIVPYLTEEQRNWEEVAVNLRKIGEQCSA----- 115 + + D + IG ++ I+P +R A +LR+ G Q +A Sbjct: 116 SPKGPNGFSGDLVKLADELHVIGAKSAIMPIFYIPERMG---AADLRQAGVQMTADEWKW 172 Query: 116 -------RGAVLS-------YHNHDFEFK--EQFGGR--TAFDGIFEEVPADLLQVEMDT 157 + A+L YHNH+FEF + GR T D + + L+ E+D Sbjct: 173 NADFLNEKAAILKKAGILAGYHNHNFEFAPLKDAKGRETTGMDILLKGTDPSLVTFELDA 232 Query: 158 CWVYYAGYDPVEYIHKYAGRLPIIHLKDMK 187 WV AG DP + Y GR +H+KD+K Sbjct: 233 GWVTAAGQDPFALLKAYPGRFTQMHVKDVK 262 >AGl2948 [G] COG1082 Sugar phosphate isomerases/epimerases Length = 353 Score = 85.9 bits (211), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 12/219 (5%), Positives = 27/219 (12%), Gaps = 22/219 (10%) Query: 1 MLKIGLQLYTLREELEQDFEGTIRKVAELGYAGVEFFHYFGRTAEQ-----VNALLQETG 55 L L + + V + Sbjct: 5 TLGNTGLLVSQLCL--GTMTFSDGTGIYEHIGDVGQTGADELVKASIDAGINFFDTADVY 62 Query: 56 LTALGAHRPYDAMLNDTEQEISFNLSIGNRNLIVPYLTEEQR-----NWEEVAVNLRKIG 110 + N L+ + + + A R Sbjct: 63 SAGASERTLGQSFKNLGIARNEIVLATKVYSRMGSGRNDVGASRGHIMDAVEASLKRLQT 122 Query: 111 EQCSARGAVLSYHNHDFEFKEQFGGRTAFDGIFEEVPADLLQVEMDTCWVYYAGYDPVEY 170 + + E + G V + + Sbjct: 123 DHIDLYQIHATDTLTPVEETLRALDDLVRQGKVRYVGV--SNWQAWRIATALGVSARLGL 180 Query: 171 IHKYAGRLPIIHLKDMKKR-----EDGSAETVVLGEGEV 204 + +R D Sbjct: 181 ERFNTLQAYYSIAGRDLERELKPLLDAEKM---GLLVWS 216 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,129,957 Number of extensions: 721819 Number of successful extensions: 1230 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1222 Number of HSP's successfully gapped: 6 Length of query: 255 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 151 Effective length of database: 26,383,695 Effective search space: 3983937945 Effective search space used: 3983937945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)