BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0011.p (386 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_citA [C] COG0372 Citrate synthase 415 e-116 BH3160 [C] COG0372 Citrate synthase 252 9e-67 CC3642 [C] COG0372 Citrate synthase 248 8e-66 BS_citZ [C] COG0372 Citrate synthase 237 3e-62 mll3842 [C] COG0372 Citrate synthase 234 1e-61 >BS_citA [C] COG0372 Citrate synthase Length = 366 Score = 415 bits (1066), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/358 (55%), Positives = 257/358 (71%), Gaps = 1/358 (0%) Query: 20 GLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQLK 79 GL+G+ ET I +DGE+G L+YRG+ AK++A+ +EE A+L+ G LPS EEL+ K Sbjct: 5 GLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEELQVFK 64 Query: 80 NEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQGEAVRLAAILP 139 +++AA R LPE+I R+I+ LP ++ M V+++ V+ALGE + P EA+RL AI P Sbjct: 65 DKLAAERNLPEHIERLIQSLPNNMDDMSVVRTVVSALGEN-TYTFHPKTEEAIRLIAITP 123 Query: 140 TIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGMNAS 199 +IIAYR R H NY YMLTGE+P+EA KAL Y IL EHGMNAS Sbjct: 124 SIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQPSEAKKKALETYMILATEHGMNAS 183 Query: 200 TFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNALEN 259 TF+ARV LSTESD+ ++V A+G MKGPLHGGAP V MLE IG KE AE +L+ LE Sbjct: 184 TFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEKLEK 243 Query: 260 GNKLMGFGHRIYKTKDPRAEALQIATQEMIGTDPSFDLALHVEAAAIRLLEEYKPGRRLF 319 G +LMGFGHR+YKTKDPRAEAL+ +E+ G D DLALHVEA AIRLLE YKPGR+L+ Sbjct: 244 GERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGRKLY 303 Query: 320 TNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYTGPMP 377 TNVEFYAAA+++A++ ++FTPTF+A R++GW AH++EQA NN IFRP + YTG +P Sbjct: 304 TNVEFYAAAVMRAIDFDDELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAIP 361 >BH3160 [C] COG0372 Citrate synthase Length = 372 Score = 252 bits (643), Expect = 9e-67, Method: Compositional matrix adjust. Identities = 144/378 (38%), Positives = 222/378 (58%), Gaps = 17/378 (4%) Query: 15 MAKVTGLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEE 74 M+ GLEGVVA T + L Y+GY ELA +EEV +LLWNG LP+ E Sbjct: 1 MSVTRGLEGVVA--TTSSVSSIIESVLTYQGYDIDELAEHASFEEVIYLLWNGKLPNKVE 58 Query: 75 LEQLKNEMAASRKLPEYIRRIIELLPAS-VPMMLVLQSTVAALG---EQGNAAWPPA-QG 129 L++L +E+A + ++P+ + ++ P V M L++ +++LG E+ + A + Sbjct: 59 LKELADELANNAEIPQEVIEQMKAYPLDRVHPMGALRTAISSLGLYDEEADLMDEAANKR 118 Query: 130 EAVRLAAILPTIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQI 189 +A++L A + T++ R AAN+LYMLTGE+P E V A + + Sbjct: 119 KALKLQAQVSTVVTAFSRIREGKEPVAPRTDLSFAANFLYMLTGEEPDEIAVDAFNKALV 178 Query: 190 LCMEHGMNASTFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERA 249 L +H +NASTF ARV ++T SD+Y+ + AIGA+KGPLHGGA +V++ML IG E Sbjct: 179 LHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEQVMAMLTEIGDVENV 238 Query: 250 EPWLRNALENGNKLMGFGHRIYKTKDPRAEALQIATQEM---IGTDPSFDLALHVEAAAI 306 EP++R AL+N K+MGFGHR+YK DPRA+ L+ ++++ G +++++ +E Sbjct: 239 EPYIRKALDNKQKIMGFGHRVYKNGDPRAKHLREMSKKLTSITGESKWYEMSVKIE---- 294 Query: 307 RLLEEYKPGRRLFTNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIF 366 E + L NV+FY+A++ +L + D+FTP F R+ GW AHI+EQ +NNR+ Sbjct: 295 ---EIVTNEKGLLPNVDFYSASVYHSLGIKHDLFTPIFAVSRMSGWLAHILEQYSNNRLI 351 Query: 367 RPQSIYTGPMPRNEFYIE 384 RP++ Y GP R +E Sbjct: 352 RPRAEYVGPNKRQYVRLE 369 >CC3642 [C] COG0372 Citrate synthase Length = 361 Score = 248 bits (634), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 149/361 (41%), Positives = 201/361 (55%), Gaps = 14/361 (3%) Query: 20 GLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQLK 79 GLEGV+A +T + VDG G LV RGY ++LA +E+ A LL +G+ A L Sbjct: 4 GLEGVIAAQTVLSDVDGAHGRLVIRGYAVEDLAGRTRFEDAAHLLLDGFFDDAPS--DLS 61 Query: 80 NEMAASR-KLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQGEAVRLAAIL 138 + + +R + + + E L P+ L++ +A + + + A+RL A Sbjct: 62 SALGEARVGVFAEVAALDEGLARRDPVE-ALRALLARIPDGDDLK------TALRLIAAP 114 Query: 139 PTIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGMNA 198 R HAA+ L M+ G T+A AL AY + +HG+NA Sbjct: 115 AVFTPAVLRVANGDAPIAPDPALSHAADILRMIRGVPATDAEAAALDAYLVTVCDHGLNA 174 Query: 199 STFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNALE 258 STFAARVV ST + + ++V + A+KGPLHGGAP VI ML+AIGT E A PWL AL Sbjct: 175 STFAARVVASTRAGLTSAVLAGLSALKGPLHGGAPGPVIDMLDAIGTPENARPWLERALA 234 Query: 259 NGNKLMGFGHRIYKTKDPRAEALQIATQEMI----GTDPSFDLALHVEAAAIRLLEEYKP 314 G++LMGFGHRIY+ +DPRA+AL+ A + + G A VE AA+ +L E+KP Sbjct: 235 RGDRLMGFGHRIYRVRDPRADALKAAVRRLSSASGGLPGRLAFAEAVERAALEILREHKP 294 Query: 315 GRRLFTNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYTG 374 R L TNVEFY A +L+AL L P FT F GR+ GW AH EQ A R+ RPQS+Y G Sbjct: 295 DRPLDTNVEFYTALLLEALGLPPSSFTCVFAMGRVAGWLAHAREQLAGGRLIRPQSVYVG 354 Query: 375 P 375 P Sbjct: 355 P 355 >BS_citZ [C] COG0372 Citrate synthase Length = 372 Score = 237 bits (604), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 138/372 (37%), Positives = 210/372 (56%), Gaps = 23/372 (6%) Query: 15 MAKVTGLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEE 74 M GLEGVVA + + + + L Y GY +L +EE+ +LLW+ LP+ +E Sbjct: 1 MTATRGLEGVVATTSSVSSIIDDT--LTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKE 58 Query: 75 LEQLKNEMAASRKLPEYIRRIIELLPA----SVPMMLVLQSTVAALG---EQGNAAWPPA 127 LE+LK ++A +P+ IIE + +V M L++ ++ LG + + P A Sbjct: 59 LEELKQQLAKEAAVPQ---EIIEHFKSYSLENVHPMAALRTAISLLGLLDSEADTMNPEA 115 Query: 128 Q-GEAVRLAAILPTIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSA 186 +A+RL A +P ++A R A N+LY L GE+P+ V+A + Sbjct: 116 NYRKAIRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTLNGEEPSPIEVEAFNK 175 Query: 187 YQILCMEHGMNASTFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTK 246 IL +H +NASTF ARV ++T SD+Y+ + AIGA+KGPLHGGA V+ ML IG Sbjct: 176 ALILHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEV 235 Query: 247 ERAEPWLRNALENGNKLMGFGHRIYKTKDPRAEALQIATQ---EMIGTDPSFDLALHVEA 303 E AEP++R LE K+MGFGHR+YK DPRA+ L+ ++ + G +++++ +E Sbjct: 236 ENAEPYIRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIED 295 Query: 304 AAIRLLEEYKPGRRLFTNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANN 363 ++L NV+FY+A++ +L + D+FTP F R+ GW AHI+EQ NN Sbjct: 296 IVTS-------EKKLPPNVDFYSASVYHSLGIDHDLFTPIFAVSRMSGWLAHILEQYDNN 348 Query: 364 RIFRPQSIYTGP 375 R+ RP++ YTGP Sbjct: 349 RLIRPRADYTGP 360 >mll3842 [C] COG0372 Citrate synthase Length = 362 Score = 234 bits (598), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 144/364 (39%), Positives = 191/364 (52%), Gaps = 13/364 (3%) Query: 19 TGLEGVVAGETDIGLVDGERGYLVYRGYWAKELAVTRCYEEVAFLLWNGYLPSAEELEQL 78 GL+ VVA ET + VDG G L RG+ ELA + +V LL +G+ E L Sbjct: 3 NGLDDVVAAETVLSDVDGLGGRLTIRGHSLAELAGRWSFPQVVRLLLDGFF------EDL 56 Query: 79 KNEMAASRKLPEYIRRIIELLPASVPMMLVLQSTVAALGEQGNAAWPPAQ--GEAVRLAA 136 + + +L E + E L S+P++ L A G A P + +A+RL A Sbjct: 57 AGDAELAAQLGEARVDVFERLQPSLPLLAPLDIYSAM--RAGMALLPDGETLADALRLIA 114 Query: 137 ILPTIIAYRYRXXXXXXXXXXXXXXXHAANYLYMLTGEKPTEAHVKALSAYQILCMEHGM 196 + R HAA+ L ML G P+ A KAL Y + +HG+ Sbjct: 115 APAVLTPALLRLQRGEQPVAPDGKAAHAADMLRMLRGSVPSPALAKALDTYLVTVCDHGL 174 Query: 197 NASTFAARVVLSTESDMYASVAGAIGAMKGPLHGGAPYEVISMLEAIGTKERAEPWLRNA 256 NASTFA RVV ST + + +SV +GA+KGPLHGGAP VI ML+AI A WLRN Sbjct: 175 NASTFATRVVASTLAGLTSSVLAGLGALKGPLHGGAPGPVIEMLDAIEAHGDAAGWLRNE 234 Query: 257 LENGNKLMGFGHRIYKTKDPRAEALQIATQEMIG---TDPSFDLALHVEAAAIRLLEEYK 313 + G ++MGFGHRIY+ +DPRA+ L+ +++ G T A VE A+ +L K Sbjct: 235 IAQGRRIMGFGHRIYRVRDPRADVLKAVVRQLGGEGETGRRLAFAEEVEKTALEVLRIAK 294 Query: 314 PGRRLFTNVEFYAAAILKALELSPDIFTPTFTAGRIIGWTAHIIEQAANNRIFRPQSIYT 373 P R L TNVEFY A +L+A P F+ F AGR+ GW AH EQ R+ RPQS Y Sbjct: 295 PQRSLQTNVEFYTALVLEAAGFPPQAFSNVFAAGRVAGWIAHAREQQTTGRLIRPQSRYV 354 Query: 374 GPMP 377 GP+P Sbjct: 355 GPVP 358 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,566,559 Number of extensions: 1018028 Number of successful extensions: 9027 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 8881 Number of HSP's successfully gapped: 59 Length of query: 386 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 278 Effective length of database: 25,922,359 Effective search space: 7206415802 Effective search space used: 7206415802 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)