BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0009.p (258 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMB2082 [L] COG0708 Exonuclease III 177 1e-44 NMA0348 [L] COG0708 Exonuclease III 177 1e-44 RSc0140 [L] COG0708 Exonuclease III 166 3e-41 XF0164 [L] COG0708 Exonuclease III 136 4e-32 SMc00956 [L] COG0708 Exonuclease III 114 1e-25 >NMB2082 [L] COG0708 Exonuclease III Length = 259 Score = 177 bits (450), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 97/256 (37%), Positives = 152/256 (59%), Gaps = 6/256 (2%) Query: 1 MTKLVSWNVNGLRACVSKGFMNYFKEAEADIFCVQETKLQEGQILLDLGEAY--EQYWNY 58 M K++S NVNG+R+ KGF Y + ADI CVQE K QE + D+ + +W+ Sbjct: 1 MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADLSADMKNPHGMHGHWHC 60 Query: 59 AVKKGYSGTAVFTKIKPLSVRYGMEVDS-EPEGRMITLEFNGFYLVNVYTPNAKRDLTRL 117 A K+GYSG AV++K KP +V+ GM ++ + EGR + +F ++++Y P+ R Sbjct: 61 AEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGRLSVISLYLPSGSSAEERQ 120 Query: 118 DYRMEWEDRFRSYLQEL-DKRKPVLVCGDLNVAHQEIDLKNAKANRGNSGFTDEERGKMT 176 + + D F L+ + ++ + ++VCGD N+AHQ IDLKN K N+ NSGF EER + Sbjct: 121 QVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLKNWKGNQKNSGFLPEEREWIG 180 Query: 177 ELLES-GFIDSFRHLHPDQEGAYTWWSYMPKIRERNVGWRIDYFLASSRLAPQLKMAGIE 235 +++ G+ D +R L+PD G YTWWS + ++VGWRIDY + + LA + A + Sbjct: 181 KVIHKLGWTDMWRTLYPDVPG-YTWWSNRGQAYAKDVGWRIDYQMVTPELAAKAVSAHVY 239 Query: 236 CNVMGSDHCPVVLQLE 251 + SDH P+V++ + Sbjct: 240 KDEKFSDHAPLVVEYD 255 >NMA0348 [L] COG0708 Exonuclease III Length = 1845 Score = 177 bits (450), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 11/256 (4%), Positives = 51/256 (19%), Gaps = 6/256 (2%) Query: 1 MTKLVSWNVNGLRACVSKGFMNYFKEAEADIFCVQETKLQEGQILLDLGEAY--EQYWNY 58 M K++ + +E +I + + + + Y Sbjct: 1 MNKILHLLFLSAFLFLVFTPGVLAEEQTTNIAYISYAPNEALEKASETNAHSDLINYTFI 60 Query: 59 AVKKGYSGTAVFTKIKPLSVRYGMEVDS-EPEGRMITLEFNGFYLVNVYTPNAKRDLTRL 117 S ++ + D + T+ + + + + Sbjct: 61 NYYNATSNGISDELLQAAETGFLETQDVIFCDYIGSTILEDPVINETLLNAHNRGTELYT 120 Query: 118 DYRMEWEDRFRSYLQEL-DKRKPVLVCGDLNVAHQEIDLKNAKANRGNSGFTDEERGKMT 176 M + Y+ + ++ + ++ + + Sbjct: 121 VRTMGKPPDYFDYISDGNSNDPICNYLNNMGTEGEGLENAENLLFYLSIKNKITYISYIP 180 Query: 177 ELLES-GFIDSFRHLHPDQEGAYTWWSYMPKIRERNVGWRIDYFLASSRLAPQLKMAGIE 235 + + +++ + + ++ G Sbjct: 181 NEALEKASETNGYRDFIEYTF-INYYNATSNGISDELLQAAETGFLETQDVIFCDYIGST 239 Query: 236 CNVMGSDHCPVVLQLE 251 + ++ Sbjct: 240 ILADPVINETLISAHN 255 >RSc0140 [L] COG0708 Exonuclease III Length = 261 Score = 166 bits (420), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 90/257 (35%), Positives = 144/257 (56%), Gaps = 7/257 (2%) Query: 1 MTKLVSWNVNGLRACVSKGFMNYFKEAEADIFCVQETKLQEGQILLDL--GEAYEQYWNY 58 M +++S N+NG+R+ SKGF + + +AD CVQE K + + + Y + + Sbjct: 1 MLRIISANLNGVRSAASKGFFEWMGKQDADFVCVQELKCAQDDMTPEFLAPHGYHGVFQH 60 Query: 59 AVKKGYSGTAVFTKIKPLSVRYGMEV-DSEPEGRMITLEFNGFYLVNVYTPNAKRDLTRL 117 AVKKGYSG ++ + +P V G + + + EGR + + +++VY P+ R Sbjct: 61 AVKKGYSGAGLYARHQPDDVIVGFDGGEFDAEGRYVEARYGKLSIISVYVPSGSSGEERQ 120 Query: 118 DYRMEWEDRFRSYLQEL--DKRKPVLVCGDLNVAHQEIDLKNAKANRGNSGFTDEERGKM 175 + + D F +L +L +K + V++CGD+N+ H+EID+KN K N+ NSG EER + Sbjct: 121 QAKYRFMDLFMQHLTDLRHEKGREVVLCGDVNIVHKEIDIKNWKGNQKNSGCLPEERAWL 180 Query: 176 TELL-ESGFIDSFRHLHPDQEGAYTWWSYMPKIRERNVGWRIDYFLASSRLAPQLKMAGI 234 T L E G++D FR L P E YTWWS + +NVGWRIDY +A+ +A + I Sbjct: 181 TRLFDEVGYVDVFRTLDPRPE-QYTWWSNRGQAYAKNVGWRIDYQIATPGIAATARSTSI 239 Query: 235 ECNVMGSDHCPVVLQLE 251 ++ SDH P+ + + Sbjct: 240 FRDIKFSDHAPLTVDYD 256 >XF0164 [L] COG0708 Exonuclease III Length = 266 Score = 136 bits (342), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 86/263 (32%), Positives = 133/263 (50%), Gaps = 17/263 (6%) Query: 3 KLVSWNVNGLRACVSKGFMNYFKEAEADIFCVQETKLQEGQIL-LDLGEA--YEQYWNYA 59 ++ S+N NGLR+ +SKGF ++F + D+ CVQETK Q Q++ D A + + + Sbjct: 2 RISSFNANGLRSALSKGFFDWFSTQDIDVLCVQETKAQRQQLVGPDFFPAGYHVCFRDAT 61 Query: 60 VKKGYSGTAVFTKIKPLSVRYGME-VDSEPEGRMITLEFNGFYLVNVYTPNAKRDLTRLD 118 KKGYSG A++++ +P +R + + + EGR + F +V+ Y P+ R Sbjct: 62 TKKGYSGVAIYSRCEPDELRTTLGWAEFDEEGRYVEARFGNLSVVSFYIPSGSSGEQRQH 121 Query: 119 YRMEWEDRFRSYLQE-LDKRKPVLVCGDLNVAHQEIDLKNAKANRGNSGFTDEERGKMTE 177 Y+ E R L E L + ++CGD N+ +D++N K+N+ NSG ER + Sbjct: 122 YKFEVMRWLRPILCEWLLSGRDYVLCGDWNIVRSALDIRNWKSNQKNSGCLPLERDWLNG 181 Query: 178 LLES-----------GFIDSFRHLHPDQEGAYTWWSYMPKIRERNVGWRIDYFLASSRLA 226 L G++D++R L+P E YTWWS R NVGWRIDY S L Sbjct: 182 LCADSLHEVNAAQGRGWVDTYRVLYPQGED-YTWWSNRGAARVNNVGWRIDYQFVSPGLR 240 Query: 227 PQLKMAGIECNVMGSDHCPVVLQ 249 ++ I SDH P ++ Sbjct: 241 ERVSRCAIYREQRFSDHAPYTVE 263 >SMc00956 [L] COG0708 Exonuclease III Length = 264 Score = 114 bits (285), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 79/262 (30%), Positives = 128/262 (48%), Gaps = 14/262 (5%) Query: 3 KLVSWNVNGLRACVSKGFMNYFKEAEADIFCVQETKLQEGQILLDLGEA--YEQYWNYAV 60 K+ ++NVNG+ + + + +EA D+ C+QE K + + + EA Y W+ Sbjct: 2 KIATYNVNGVNGRLGV-LLRWLEEASPDVVCLQELKAPDPKFPVKAIEAAGYGAIWH--G 58 Query: 61 KKGYSGTAVFTKIK-PLSVRYGMEVDSE-PEGRMITLEFNGFYLVNVYTPNAKR-DLTRL 117 +K ++G A+ + + P R G+ D + R I G + +Y PN + Sbjct: 59 QKSWNGVAILARDREPTLTRKGLPGDPDDTHSRYIEAAVEGMVIGCLYLPNGNPYPGPKF 118 Query: 118 DYRMEWEDRFRSYLQEL-DKRKPVLVCGDLNVAHQEIDLKNAKANRGNSGFTDEERGKMT 176 +Y++ W R +Y EL + PV++ GD NV E+D+ + ++ F E R Sbjct: 119 EYKLAWFHRLTAYAAELLELDVPVILAGDYNVMPTELDVYKPERWVNDALFRIEVRDAYH 178 Query: 177 ELLESGFIDSFRHLHPDQEGAYTWWSYMPKIRERNVGWRIDYFLASSRLAPQLKMAGIEC 236 LLE G+ D+ R LHP E YT+W Y R+ G RID+ L S + +L AG++ Sbjct: 179 RLLEQGWTDALRQLHPG-ERVYTFWDYFRNAFARDAGLRIDHLLLSPHVTLRLSAAGVDR 237 Query: 237 NVMG----SDHCPVVLQLEDTP 254 +V G SDH P ++L D P Sbjct: 238 HVRGWEHTSDHAPAWIELSDGP 259 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,560,971 Number of extensions: 826103 Number of successful extensions: 1236 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 1158 Number of HSP's successfully gapped: 32 Length of query: 258 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 154 Effective length of database: 26,383,695 Effective search space: 4063089030 Effective search space used: 4063089030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)