BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0002.p (447 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP2083 [T] COG0642 Signal transduction histidine kinase 84 4e-16 lin2727 [T] COG0642 Signal transduction histidine kinase 82 1e-15 PA0757 [T] COG0642 Signal transduction histidine kinase 82 2e-15 lin1415 [T] COG0642 Signal transduction histidine kinase 81 3e-15 RSc1735 [T] COG0642 Signal transduction histidine kinase 78 3e-14 >SP2083 [T] COG0642 Signal transduction histidine kinase Length = 443 Score = 84.3 bits (207), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 61/224 (27%), Positives = 111/224 (49%), Gaps = 13/224 (5%) Query: 222 IQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVVTQET----GLDVIMNQSLRLID 277 I+ +++ + F+ N SHEL+TP+ SI GF I+ +E LD+I +SLRL Sbjct: 211 IRTYEKLNLAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEH 270 Query: 278 MVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXXXXXXXRGVALKVE-GESLLTVAA 336 +V+ LL LS+ + + W + + L + + LKV+ + + V+ Sbjct: 271 IVEHLLTLSKAQQMPIQW--TTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSD 328 Query: 337 GEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAEREAVFQRFY 396 + A+R+T +++++ G I + D G G+S+ E++ +F+RFY Sbjct: 329 SQLLSQILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFY 388 Query: 397 ------TGPNGVTGLGLAICRQIAANLDGELTYSSSPLGGARFS 434 + G TGLGLAI ++++ L G++T +S G+ F+ Sbjct: 389 RVNKGRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFT 432 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 82.4 bits (202), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 0/290 (0%), Positives = 0/290 (0%), Gaps = 12/290 (4%) Query: 155 LLAIGFSFFMILAIGLLTVS--RVVRPLNRLKEAVSRYEPYRSQDAVFPVDDTTEIGELI 212 Sbjct: 164 - 222 Query: 213 TTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVVT---QETGLDVIM 269 Sbjct: 223 282 Query: 270 NQSLRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXXXXXXXRGVALKVEGE 329 Sbjct: 283 342 Query: 330 SLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAERE 389 Sbjct: 343 402 Query: 390 AVFQRFY------TGPNGVTGLGLAICRQIAANLDGELTYSSSPLGGARF 433 Sbjct: 403 452 >PA0757 [T] COG0642 Signal transduction histidine kinase Length = 460 Score = 81.6 bits (200), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 82/299 (27%), Positives = 137/299 (45%), Gaps = 31/299 (10%) Query: 149 SLMRTTLLAIGFSFFMILAIGLLTVSRVVRPLNRLKEAVSRYEPYRSQDAVFPV---DDT 205 SLM TLL +G L + L VS +RPL+RL+ V +P D + P+ + Sbjct: 166 SLMTDTLLRLGVLGVGALVLVWLAVSAALRPLDRLRREVEERQP----DDLRPLPMLEVQ 221 Query: 206 TEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFV-FAIQDQVV-TQET 263 E+ L+ + ++R++ Q F+ + +HEL+TPL ++ + +++Q + Sbjct: 222 HELRPLVESLNHFTQRLRSLFERQSHFIADAAHELRTPLAALKARLELGLREQEPEAWRS 281 Query: 264 GLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHWPISE-----IDLRTXXXXXXXXXXXXXX 318 L+ + RLI + ++LL L+R+E+ + I+E IDL Sbjct: 282 TLETTAKNTDRLIHLANQLLSLARIESGAQA--IAEGGAERIDLTALARELGLALAPLAY 339 Query: 319 XRGVALKVEGESLLTVAAGEXXXXXXXXXXXX--AVRHTSSQ----IRVQIEAGYPGAGW 372 RG +L +E + + GE A+ HT +RV +E G Sbjct: 340 ARGASLALESAGPVWIQ-GEPTLLNELLSNLLDNALAHTPQGGNVILRV-LEGG------ 391 Query: 373 AIHIDDDGNGLSEAEREAVFQRFY-TGPNGVTGLGLAICRQIAANLDGELTYSSSPLGG 430 + ++DDG G+ A+RE VFQRFY G + +GLGLAI ++ ++ LGG Sbjct: 392 VLEVEDDGPGIPAADRERVFQRFYRRGDSPGSGLGLAIVGEVCRAHRAQIQLDQGELGG 450 >lin1415 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 80.9 bits (198), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 0/304 (0%), Positives = 0/304 (0%), Gaps = 11/304 (3%) Query: 139 PLSSLRALYGSLMRTTLLAIGFSFFMILAIGLLTVSRVVRPLNRLKEAVSRYEPYRSQDA 198 Sbjct: 166 225 Query: 199 V-FPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQ 257 Sbjct: 226 285 Query: 258 VVTQETGLDVIMNQSL----RLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXX 313 Sbjct: 286 345 Query: 314 XXXXXXRGVALKVEGESLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWA 373 Sbjct: 346 405 Query: 374 IHIDDDGNGLSEAEREAVFQRFY------TGPNGVTGLGLAICRQIAANLDGELTYSSSP 427 Sbjct: 406 465 Query: 428 LGGA 431 Sbjct: 466 469 >RSc1735 [T] COG0642 Signal transduction histidine kinase Length = 471 Score = 77.8 bits (190), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 79/285 (27%), Positives = 117/285 (41%), Gaps = 29/285 (10%) Query: 166 LAIGLLTVSRVVRPLNRLKEAVSRYEPYRSQDAVFPVDD---TTEIGELITTFQSMSERI 222 L I L V R +RPL +L +V+ R D + P+ E+ L+ R+ Sbjct: 180 LGIVWLGVRRGLRPLRQLSASVA----ARHADELAPLPQHGLPAEVTPLVAAINQYVARL 235 Query: 223 QQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVVTQETGLDVIMNQSLRLIDMV--- 279 + + F + +H+LKTPL I Q ++ +E + I L D V Sbjct: 236 LRMLDARKRFFADAAHQLKTPLAVIQA-----QSELALREPDGNRIRRHLAPLHDTVRQA 290 Query: 280 ----DKLLQLSRLEAVDEHWP-ISEIDLRTXXXXXXXXXXXXXXXRGVALKVEGESLLTV 334 +LL LSRLE + P + + L T G+ L EGE + Sbjct: 291 AKGVQQLLSLSRLEPDSGYAPSLQPLRLDTLAGEVALELAPVAHRAGIDLGFEGEPVTVA 350 Query: 335 AAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAG---YPG--AGWAIHIDDDGNGLSEAERE 389 A + A+R+ RV + PG G + ++D+G G++EAER Sbjct: 351 AEPQWLQELVGNLLDNAIRYAGPDARVTLRVARQAAPGQDTGALLQVEDNGPGIAEAERA 410 Query: 390 AVFQRFYTGPNGV----TGLGLAICRQIAANLDGELTYSSSPLGG 430 AVF RFY G +GLGLAI R+IA + S + GG Sbjct: 411 AVFGRFYRGSTAQAQEGSGLGLAIVREIARVHGATVALSDTAGGG 455 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,976,022 Number of extensions: 1118041 Number of successful extensions: 3332 Number of sequences better than 1.0e-05: 82 Number of HSP's gapped: 3321 Number of HSP's successfully gapped: 82 Length of query: 447 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 337 Effective length of database: 25,691,691 Effective search space: 8658099867 Effective search space used: 8658099867 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 117 (49.7 bits)