BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0001.p (423 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2245 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 441 e-123 lin1823 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 368 e-101 DR2188 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 354 2e-97 DR0236 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) 321 1e-87 AGc4702 [S] COG5476 Uncharacterized conserved protein 310 2e-84 >BH2245 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 410 Score = 441 bits (1133), Expect = e-123, Method: Compositional matrix adjust. Identities = 209/410 (50%), Positives = 269/410 (65%) Query: 14 MLDFDVMLEKYADLVVRVGVNVQPGQVLMIHSPLETAELTRLIVGKAYEAGAKYVLVDWD 73 M F LEKYA+L V+VGVN+QPGQ L+I +P+ EL RL+ KAYEAGAK V V+W Sbjct: 1 MTTFQENLEKYAELAVKVGVNIQPGQTLVIRTPISALELVRLVAKKAYEAGAKNVHVEWS 60 Query: 74 DEKVTRIRYEKAADDSFGYYPQWQADMLEGFAEEGGAILHIKVPDPELFRGIDXXXXXXX 133 DE++TR ++ A D++F YP W A E AE G A L I DP+L + D Sbjct: 61 DEELTRTKFHLAPDEAFHEYPAWIAKGFEELAENGAAFLSITGQDPDLLKDADPERVANL 120 Query: 134 XXXXXXXXXXYQNYTRNNKISWSLIKAPTKAWADKVYAGLPEEERVAAMWEAVFQMNRVG 193 +++Y +K+SWS++ AP++ WA K+ ++ V +WEA+F R Sbjct: 121 NKAGGKALKTFRSYIMADKVSWSIVAAPSEGWAKKISPDESKDRAVEKLWEAIFAATRTD 180 Query: 194 SGDPVAAWREHIADLKKSQDRMNAKRYKSLHYRAPGTDLHVELPEGHLWRGGGGENASGV 253 DPVAAW+ H+ L + + +N +RY +LHY+APGTDL +ELP+ HLW GG N GV Sbjct: 181 QADPVAAWKNHLKTLNEKMNELNKRRYHALHYKAPGTDLTIELPDTHLWVSGGSTNKDGV 240 Query: 254 YFVANMPTEEVYTMPHRKGVNGTVSSTLPLNLNGRLVDGITITFTDGKVTAYDAESGREH 313 FVAN+PTEEV+T + GVNGTV++T PLN G L+D T+TF DGKV + AE G E Sbjct: 241 DFVANIPTEEVFTAAKKTGVNGTVTNTKPLNYGGTLIDQFTLTFKDGKVVDFTAEQGYET 300 Query: 314 LTSLLETDEGASYLGEVALVPHDSPISRLNTVFYNTGIDENASCHFALGSAYPSNIEGGT 373 L LL+TDEGA Y+GEVALVPH SPIS + +FYNT DENAS H A+G+AY NIEGG Sbjct: 301 LKRLLDTDEGARYIGEVALVPHQSPISETDLIFYNTLFDENASNHLAIGTAYAFNIEGGK 360 Query: 374 SLSSEELLERGANVSLTHVDFMVGSAELDIDGELADGTVEPVFRKGNWVL 423 +S EEL + G N S+THVDFM+GS+E+DIDG DG EPVFRKGNW Sbjct: 361 EMSEEELEQAGINTSITHVDFMIGSSEMDIDGITKDGKREPVFRKGNWAF 410 >lin1823 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 410 Score = 368 bits (944), Expect = e-101, Method: Compositional matrix adjust. Identities = 182/410 (44%), Positives = 250/410 (60%) Query: 14 MLDFDVMLEKYADLVVRVGVNVQPGQVLMIHSPLETAELTRLIVGKAYEAGAKYVLVDWD 73 M F LEKYA+L+V+VGVNVQP Q ++I +P++ A L RLI A+E GA+ V++DW Sbjct: 1 MTVFSEKLEKYAELIVKVGVNVQPEQKVVIMAPVDAAPLVRLISKYAFEVGAEDVIMDWR 60 Query: 74 DEKVTRIRYEKAADDSFGYYPQWQADMLEGFAEEGGAILHIKVPDPELFRGIDXXXXXXX 133 DE++ +RY+ A F P + A+EG + I DP+L G+D Sbjct: 61 DEELGALRYKNAPLRVFESAPVHRVAEKTELAKEGACFISITSEDPDLLNGVDSNKIATF 120 Query: 134 XXXXXXXXXXYQNYTRNNKISWSLIKAPTKAWADKVYAGLPEEERVAAMWEAVFQMNRVG 193 ++ + N +SW+++ A ++ WA KV+ L E++ +WEA+F+ R+ Sbjct: 121 QKTMGGAMSEFRELMQANVVSWTVVAAASQGWAAKVFPDLTPAEQMETLWEAIFETTRIN 180 Query: 194 SGDPVAAWREHIADLKKSQDRMNAKRYKSLHYRAPGTDLHVELPEGHLWRGGGGENASGV 253 + +PV W+ H L + +N K++ SLHY APGTDL + LP+ HLW G G +N G Sbjct: 181 TENPVETWKNHDQTLAAKAESLNEKQFTSLHYTAPGTDLTIGLPKNHLWVGAGSKNKKGH 240 Query: 254 YFVANMPTEEVYTMPHRKGVNGTVSSTLPLNLNGRLVDGITITFTDGKVTAYDAESGREH 313 F+ANMPTEEV+ + V G VSST PL+ G ++D ITF G++ +A SG E Sbjct: 241 EFMANMPTEEVFCCADKLKVEGYVSSTKPLSYAGNIIDDFKITFEKGRIVGVEAASGEEI 300 Query: 314 LTSLLETDEGASYLGEVALVPHDSPISRLNTVFYNTGIDENASCHFALGSAYPSNIEGGT 373 L L+ TDEG+ YLGEVALVP SPIS+ +FYNT DENAS H A+GSAY N++GG Sbjct: 301 LKDLIATDEGSHYLGEVALVPDPSPISQSGILFYNTLFDENASNHLAIGSAYAFNVKGGE 360 Query: 374 SLSSEELLERGANVSLTHVDFMVGSAELDIDGELADGTVEPVFRKGNWVL 423 +S EEL G N SLTHVDFM+GS+E+DIDG G V PVFRKG+W Sbjct: 361 EMSREELEAAGVNNSLTHVDFMIGSSEMDIDGVTESGEVVPVFRKGDWAF 410 >DR2188 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 417 Score = 354 bits (908), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 182/409 (44%), Positives = 252/409 (61%) Query: 15 LDFDVMLEKYADLVVRVGVNVQPGQVLMIHSPLETAELTRLIVGKAYEAGAKYVLVDWDD 74 L F+ L+ YA L VRVG+ V+PGQ +++ SP++ A L RLIV +AY AGA++V V WDD Sbjct: 9 LSFEQKLQNYARLAVRVGLGVKPGQRVLVQSPVDAAPLARLIVREAYAAGARFVDVRWDD 68 Query: 75 EKVTRIRYEKAADDSFGYYPQWQADMLEGFAEEGGAILHIKVPDPELFRGIDXXXXXXXX 134 + V R+E A D +F QW+ D A GGA++ I+ DP L +D Sbjct: 69 DDVQLARFELAPDGTFEEISQWRVDAELETANAGGAVIAIRATDPNLLAKVDPERVATWQ 128 Query: 135 XXXXXXXXXYQNYTRNNKISWSLIKAPTKAWADKVYAGLPEEERVAAMWEAVFQMNRVGS 194 Y N+++W+L+ AP WA ++ E+ V W+A+F R Sbjct: 129 RANATYRKPYSLQVMTNRLNWNLVSAPIPGWATLMFPEASSEQAVEQQWDAIFAAVRADQ 188 Query: 195 GDPVAAWREHIADLKKSQDRMNAKRYKSLHYRAPGTDLHVELPEGHLWRGGGGENASGVY 254 DPVA W EH+A+LK+ ++ + K+Y +LH+R TDL V L + H+W GG + SGV Sbjct: 189 PDPVALWEEHLANLKRRREILTGKQYHALHFRGGDTDLTVGLADDHIWGGGAADTPSGVT 248 Query: 255 FVANMPTEEVYTMPHRKGVNGTVSSTLPLNLNGRLVDGITITFTDGKVTAYDAESGREHL 314 F AN+PTEEV+T PHR+ V+G V ST PL+ G L+DGI I F DG++T A+ G L Sbjct: 249 FTANIPTEEVWTAPHRERVDGVVVSTKPLSYAGTLIDGIRIEFKDGRITGASAKQGEAAL 308 Query: 315 TSLLETDEGASYLGEVALVPHDSPISRLNTVFYNTGIDENASCHFALGSAYPSNIEGGTS 374 +++TDEG+ LGEVALVPH SPISR FYNT DENA+ H A+GSAY N++GG Sbjct: 309 LKMIDTDEGSHRLGEVALVPHSSPISRSGLFFYNTLYDENAASHIAIGSAYRFNVKGGID 368 Query: 375 LSSEELLERGANVSLTHVDFMVGSAELDIDGELADGTVEPVFRKGNWVL 423 + +E+ ++G N SLTHVD+MVGS ++D+DG DG EPV R G +V+ Sbjct: 369 MDAEQFAQKGGNDSLTHVDWMVGSDQIDVDGITKDGNREPVMRAGEFVI 417 >DR0236 [E] COG2309 Leucyl aminopeptidase (aminopeptidase T) Length = 411 Score = 321 bits (823), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 158/408 (38%), Positives = 242/408 (59%), Gaps = 3/408 (0%) Query: 17 FDVMLEKYADLVVRVGVNVQPGQVLMIHSPLETAELTRLIVGKAYEAGAKYVLVDWDDEK 76 FD L +YA+L+V+VGVN+ G L++++PL A L RL+ AY AGA V V++ DE Sbjct: 6 FDERLARYAELLVKVGVNLPAGGKLLVNAPLSAAPLVRLVARAAYRAGALDVRVEYHDEH 65 Query: 77 VTRIRYEKAADDSFGYYPQWQADMLEGFAEEGGAILHIKVPDPELFRGIDXXXXXXXXXX 136 + YE +D++ G+ P W + ++ ++G A L I+ DP L + ++ Sbjct: 66 LALALYEDGSDEAMGFLPPWATEQVQHMLDDGYARLSIRGDDPALLKNVNPERIARRSKL 125 Query: 137 XXXXXXXYQNYTRNNKISWSLIKAPTKAWADKVYAGLPEEERVAAMWEAVFQMNRVGSGD 196 ++W++ T AWA VY LPE E VA +W+ +F++ R D Sbjct: 126 MAQASREVSAAVSGFAVNWTVAAMSTPAWAQAVYPELPEAEAVACLWDDIFKVTRADLPD 185 Query: 197 PVAAWREHIADLKKSQDRMNAKRYKSLHYRAP-GTDLHVELPEGHLWRGGGGENASGVYF 255 P+ AW +H+A L + +N K+Y ++H GTDL V L E H+W+GG E +G+ Sbjct: 186 PLGAWEDHVAALARRSAHLNDKQYAAVHLTTELGTDLTVGLAERHIWQGGAEEAKNGIVG 245 Query: 256 VANMPTEEVYTMPHRKGVNGTVSSTLPLNLNGRLVDGITITFTDGKVTAYDAESGREHLT 315 V N+PT+EV+TMPHR VNG ++ PL++ G++V+GI + F +GK AE G + L Sbjct: 246 VPNLPTDEVFTMPHRDRVNGVAVASKPLSVRGQVVEGIRVRFENGKAVEVSAEKGEDTLK 305 Query: 316 SLLETDEGASYLGEVALVPHDSPISRLNTVFYNTGIDENASCHFALGSAYPSNIEGGTSL 375 SL+ TDEGA++LGE+ALV +P+++ T+F+NT DENA+ H ALG YP+N++GG Sbjct: 306 SLIATDEGAAHLGEIALVAASAPVAQTGTLFFNTLFDENAASHIALGRCYPTNVQGGA-- 363 Query: 376 SSEELLERGANVSLTHVDFMVGSAELDIDGELADGTVEPVFRKGNWVL 423 +++ G N SL HVD+M+G+ +DG ADG EP+ R G W++ Sbjct: 364 DAQQRQAAGGNDSLIHVDWMIGTPGASVDGITADGQREPLMRGGEWMI 411 >AGc4702 [S] COG5476 Uncharacterized conserved protein Length = 412 Score = 310 bits (795), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 168/406 (41%), Positives = 227/406 (55%), Gaps = 6/406 (1%) Query: 19 VMLEKYADLVVRVGVNVQPGQVLMIHSPLETAELTRLIVGKAYEAGAKYVLVDWDDEKVT 78 V LEK A++ ++VG+ +Q Q L+I +PL L RL+ AY AG V + DE+ T Sbjct: 9 VKLEKLAEVAIKVGLQLQKDQDLVITAPLAALPLVRLLTKHAYIAGGGLVTTFYSDEETT 68 Query: 79 RIRYEKAADDSFGYYPQWQADMLEGFAE---EGGAILHIKVPDPELFRGIDXXXXXXXXX 135 RY A+D +F W + EG A+ G A L I +P L D Sbjct: 69 LSRYRHASDANFDRASGW---LYEGMAKAYANGAARLAIAGDNPMLLAEEDPAKVARANK 125 Query: 136 XXXXXXXXYQNYTRNNKISWSLIKAPTKAWADKVYAGLPEEERVAAMWEAVFQMNRVGSG 195 N I+W++I P +WA +V+ GL E+E V + +A+F +RV Sbjct: 126 ANSTAYKPALEKISNFDINWNIISYPNPSWAKQVFPGLTEDEAVRKLADAIFAASRVDVA 185 Query: 196 DPVAAWREHIADLKKSQDRMNAKRYKSLHYRAPGTDLHVELPEGHLWRGGGGENASGVYF 255 DPVAAW +H A+L K +N +R+ +LH+ PGTD+ + L +GH W GG +GV Sbjct: 186 DPVAAWAQHNANLAKRSAWLNGERFSALHFTGPGTDVKIGLADGHEWHGGASMAKNGVTC 245 Query: 256 VANMPTEEVYTMPHRKGVNGTVSSTLPLNLNGRLVDGITITFTDGKVTAYDAESGREHLT 315 N+PTEEV+T PH V+G VSST PL+ G L+D I + F G++ A G L Sbjct: 246 NPNIPTEEVFTTPHALRVDGYVSSTKPLSHQGTLIDDIQVKFEAGRIVEAKASKGEAVLN 305 Query: 316 SLLETDEGASYLGEVALVPHDSPISRLNTVFYNTGIDENASCHFALGSAYPSNIEGGTSL 375 +L+TDEGA LGEVALVPH SPIS +FYNT DENASCH ALG Y G SL Sbjct: 306 KVLDTDEGARRLGEVALVPHSSPISASGILFYNTLFDENASCHIALGQCYSKCFLDGASL 365 Query: 376 SSEELLERGANVSLTHVDFMVGSAELDIDGELADGTVEPVFRKGNW 421 S +++ +G N SL H+D+M+GS ++DIDG DG+ PV RKG W Sbjct: 366 SQDQIKAQGGNSSLIHIDWMIGSNKVDIDGVKPDGSTVPVMRKGEW 411 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,405,934 Number of extensions: 1422658 Number of successful extensions: 4327 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 4313 Number of HSP's successfully gapped: 12 Length of query: 423 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 314 Effective length of database: 25,807,025 Effective search space: 8103405850 Effective search space used: 8103405850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)