BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0210.0004.p (503 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAP0114 [G] COG3507 Beta-xylosidase 317 2e-86 BH3683 [G] COG3507 Beta-xylosidase 125 1e-28 CC2802 [G] COG3507 Beta-xylosidase 115 1e-25 CC0989 [G] COG3507 Beta-xylosidase 112 2e-24 BS_xynB [G] COG3507 Beta-xylosidase 95 3e-19 >CAP0114 [G] COG3507 Beta-xylosidase Length = 531 Score = 317 bits (813), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 194/522 (37%), Positives = 275/522 (52%), Gaps = 53/522 (10%) Query: 1 LNTAKITNPVIWADVPDVDVIRVGPVFYMVSTSMHSMPGCPIMKSVNLKDWEIVGYVYDT 60 L NPV++AD PD VI+VG +YM +S++ MPG PI+KS +L +WEI Y +D Sbjct: 37 LGNGTYQNPVMYADYPDNSVIKVGSTYYMACSSINYMPGLPILKSQDLVNWEICSYAFDR 96 Query: 61 FEEN---------EAHRLEDGEGIYGSGSWAASLRHKNGTFYVCFSSNDMDQ-FYIYQTR 110 + +A+ L GE +Y G WA SL++ NGTFY F+S D+ + F +T Sbjct: 97 IDTGMSGIDKTHADAYNLVGGENVYSKGCWAPSLKYHNGTFYATFASLDLGKTFVCTKTG 156 Query: 111 DIEHGPWERSVIPGL--YHDPALLLDDDGRNYVIYGNGDIRVQELTADLTAVKPGGTDQL 168 + G W + I G+ HD L D DGR Y+I NG V ELT D +VK GG +Q Sbjct: 157 PMGFGGWTFTAISGIGYAHDADLFFDTDGRVYLI--NGGCNVTELTPDCKSVKYGGINQK 214 Query: 169 LLEGERKDIMLRIEGCHAHKRDGYYFLFFIEWPSAGNQRRRQLCYRSRELLGPYEHRVVL 228 + +G +G K++GYY++ G +R + +RS+ L GPYE ++L Sbjct: 215 IFDGGPSH-----DGNRIFKKNGYYYILSTPIKGNGAYQRIEKAWRSKSLTGPYEQTIIL 269 Query: 229 DDDLGYRNNGVAQGGIVDTPDG-YWYAVLFQDHGAVGRIPCVFPLTWENNWPVIGDGGKA 287 DD + Q +VD DG Y +A+LF+D GAVGRIP + P+TW NNWP+IG G A Sbjct: 270 DDGANH------QVCVVD--DGSYNWAMLFEDKGAVGRIPKLAPVTWVNNWPMIGVNGSA 321 Query: 288 PL-SFETKLPAGEPKPLVISDEFEYTENRLALQWQWNHNPDNGLWSVTERPGYLRLRTGR 346 + + K AG +D+ ++ + QWQWNHNPDN W+ +ERPG++RL+T Sbjct: 322 TVPNIYNKPAAGNGIKSPGTDDDFTSQGYVLSQWQWNHNPDNSKWTTSERPGWMRLKT-S 380 Query: 347 IADDVLLARNTLTQRTEGPASSAETMLHVSGLSAGDRAGMVALQNGYGTVEVAAGEQGQL 406 + D L ARNTLTQR +GPASS L + + G +G+ A + YG + V Sbjct: 381 YSKDFLQARNTLTQRIQGPASSGWVKLDTTNIKPGQISGLSAFHSKYGYIGV-------- 432 Query: 407 DVNMCINGDKGTEVVESVPFTGERIGLRIDFNF-------EDGVDEARFFYSGDGEVWHP 459 D GT+ + TG + + N + A+F+YS +G W Sbjct: 433 ------RNDNGTKKLVQYNVTGSETAINLTNNIVYLKVSTDCNAQTAKFYYSYNGNSWTQ 486 Query: 460 IGQTLHMKYTLDH-FMGYXXXXFNYAT-RQPGGYADFDYFRY 499 +G TL M + F+GY FNY T GYADFDYF++ Sbjct: 487 LGSTLKMDFFYKLWFVGYRFALFNYTTGSDTSGYADFDYFKF 528 >BH3683 [G] COG3507 Beta-xylosidase Length = 528 Score = 125 bits (315), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 140/542 (25%), Positives = 231/542 (42%), Gaps = 69/542 (12%) Query: 5 KITNPVIWADVPDVDVIRVGPVFYMVSTSMHSMPGCPIMKSVNLKDWEIVGYVYDTFEEN 64 +I NP++ PD ++RVG +Y+ +++ PG I S +LK W V + Sbjct: 4 RIQNPILPGFHPDPSIVRVGDDYYIATSTFEWFPGVRIHHSRDLKHWRFV-----SSPLT 58 Query: 65 EAHRLEDGEGIYGSGSWAASLRHKNGTFYVCFSSNDMDQF---------YIYQTRDIEHG 115 +L+ + G WA L + +GTFY+ ++ D+ Q+ Y+ ++IE G Sbjct: 59 RTSQLDMKGNMNSGGIWAPCLSYHDGTFYLIYT--DVKQWHGAFKDAHNYLVTAQNIE-G 115 Query: 116 PWERSV-IPGLYHDPALLLDDDGRNYVIYGNGDIRVQELTADLTAVKPGGTDQLLLEGER 174 PW + + DP+L DDDGR +++ D R ++ + L G Sbjct: 116 PWSDPIYLNSSGFDPSLFHDDDGRKWLVNMIWDYRKGNHPFAGIILQEYSEAEQKLVGPV 175 Query: 175 KDI-----MLRIEGCHAHKRDGYYFLFFIEWPSAGNQRRRQLCYRSRELLGPYE----HR 225 K+I + EG H +K+DGYY+L E RS+ + GPYE + Sbjct: 176 KNIYKGTDIQLTEGPHLYKKDGYYYLLVAE--GGTEYEHAATLARSQSIDGPYETDPSYP 233 Query: 226 VVLDD---DLGYRNNGVAQGGIVDTPDGYWYAV------LFQDHGAVGRIPCVFPLTW-E 275 +V +L + G G +V+T +G WY L + +GR + + W E Sbjct: 234 LVTSTGQPELALQKAG--HGSLVETQNGEWYLAHLCGRPLKGKYCTLGRETAIQKVNWTE 291 Query: 276 NNWPVIGDGGKAPLSFETKLPAGEPKPLVISDEF-EYTENRLALQWQWNHNPDNGLW-SV 333 + W I DGG PL E P P E ++ +L QW P + W S+ Sbjct: 292 DGWLRIEDGGNHPLR-EVTAPDLPEHPFEKEPELDDFDAPQLHHQWNTLRIPADPSWCSL 350 Query: 334 TERPGYLRLRTGRIADDVLLARNTLTQRTEGPASSAETMLHVSGLSAGDRAGMVALQNGY 393 ERPG+LRLR G + + +++ + +R + ET L S AG+V + Sbjct: 351 EERPGHLRLR-GMESLTSVHSQSLVARRQQSFHCEVETKLEYQPESFQHMAGLVIYYDTE 409 Query: 394 GTVEVAAG---EQGQ-LDVNMCINGDKGTEVVESVPFTGER-IGLRIDFNFEDGVDEARF 448 V + E+G+ L + G+ + +P E+ + L+ + E Sbjct: 410 DHVYLHVTWHEEKGKCLQIIQTKGGNYDELLASPIPLAEEKAVYLKGRIHRET----MHL 465 Query: 449 FYSGDGEV-WHPIGQTLHMKYTLDH----------FMGYXXXXFNYATRQPGGYADFDYF 497 ++ +GE W P+G T+ + + D F+G + T++P ADFDYF Sbjct: 466 YFKQEGEAEWQPVGPTIDVTHMSDDSAKQVRFTGTFVGMATQDLS-GTKKP---ADFDYF 521 Query: 498 RY 499 RY Sbjct: 522 RY 523 >CC2802 [G] COG3507 Beta-xylosidase Length = 548 Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 117/420 (27%), Positives = 174/420 (41%), Gaps = 48/420 (11%) Query: 8 NPVIWADVPDVDVIRVGPVFYMVSTSMHSMPGCPIMKSVNLKDWEIVGYVYDTFEENEAH 67 NPV+ PD V R G FY+V+++ PG P+ S +L WE +G D + Sbjct: 37 NPVVQGFYPDPSVTRAGKDFYLVNSTFAWFPGLPVFHSTDLVHWEQIGNAIDRPGMLDFK 96 Query: 68 RLEDGEGIYGSGSWAASLRHKNGTFYVCFSSNDMDQFYIYQTRDIEHGPWERSV-IPGLY 126 RL ++ A +++ G FY+ + D Y+ +D GPW V +P + Sbjct: 97 RLGLSRAVF-----APAIQAHQGMFYILNTCVDCGGNYLITAKD-PKGPWSDPVWLPKVG 150 Query: 127 H-DPALLLDD-DGRNYVIYGNGDIRVQELTA-----------DLTAVKPGGTDQLLLEGE 173 DP++ DD DG V N D D G LLL+ Sbjct: 151 GIDPSIFFDDEDGGGRVWIVNNDGPPGPPEYPGHRALWIHEYDPKTQTTVGPATLLLDKG 210 Query: 174 RKDIMLRI--EGCHAHKRDGYYFLFFIEWPSAGNQRRRQLCYRSRELLGPY----EHRVV 227 I EG H K+DG+Y+L E +A + Q+ RS+ + GPY + ++ Sbjct: 211 VDPATKPIWPEGPHILKKDGWYYLTAAEGGTA--EGHSQVVLRSKSVTGPYVPYTNNPIL 268 Query: 228 LDDDLGY-RNNGVAQGG---IVDTPDGYWYAVLFQ------DHGAVGRIPCVFPLTWENN 277 DL R + G IVDTPDG W+A D GR + P+ W++ Sbjct: 269 TQRDLPRDRPLPITSAGHADIVDTPDGKWWATFLAVRPYGDDLYNTGRETFLLPVEWKDG 328 Query: 278 WPVIGDGGKAP--LSFETKLPAGEPKPLVISDEFEYTEN----RLALQWQWNHNPDNGLW 331 WPVI + GK ++ KLPA + P S F+ E L + W P W Sbjct: 329 WPVILENGKTIPYVADAPKLPALKTAP--TSGPFKAAETFEGPELPMSWMTMRIPSKRWW 386 Query: 332 SVTERPGYLRLRTGRIADDVLLARNTLTQRTEGPASSAETMLHVSGLSAGDRAGMVALQN 391 SV + L R R+ + +R + +S T + + GD+AG+VA+QN Sbjct: 387 SVKDGTLSLEARKDRVGGHE--QPSIWARRQQHMNASVSTTVRFDPKADGDKAGLVAVQN 444 >CC0989 [G] COG3507 Beta-xylosidase Length = 546 Score = 112 bits (279), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 139/554 (25%), Positives = 217/554 (39%), Gaps = 76/554 (13%) Query: 6 ITNPVIWADVPDVDVIRVGPVFYMVSTSMHSMPGCPIMKSVNLKDWEIVGYVYDTFEENE 65 I NPV+ PD ++RVG +Y+ +++ PG I S +L++W ++G ++ Sbjct: 6 IRNPVLRGFNPDPSIVRVGEDYYIATSTFEWFPGVQIHHSRDLRNWRLIGRPLR--RPSQ 63 Query: 66 AHRLEDGEGIYGSGSWAASLRHKNGTFYVCF--------------SSNDMDQFYIYQ-TR 110 + + D +G G WA L H +G F++ F S + F+ Y T Sbjct: 64 LNMMGDPDGC---GVWAPCLTHADGRFWLIFTDVKRYGRTTVGGASGASLRDFHNYLVTC 120 Query: 111 DIEHGPWERSV-IPGLYHDPALLLDDDGRNYVIYGNGDIRVQELTADLTAVKPGGTDQLL 169 + G W + + DP+L DDDGR +++ D R ++ + Sbjct: 121 ETIDGDWSDPIHLNSSGFDPSLFHDDDGRKWLVNQLWDHRPGRNRFAGIVLQAFSVAEQR 180 Query: 170 LEGERKDI-----MLRIEGCHAHKRDGYYFLFFIE----WPSAGNQRRRQLCYRSRELLG 220 L GERK I + E H +KRDG+Y+L E W A RSR + G Sbjct: 181 LVGERKVIFEGTAIGLTEAPHLYKRDGWYYLLTAEGGTGWGHAAT------LARSRAIDG 234 Query: 221 PYE-----HRVVLDDDLGYRNNGVAQGGIVDTPDGYWYAVLFQDHGAVGRIPCVF----- 270 PYE + + D G + +T DG Y GR CV Sbjct: 235 PYELHPDTYLLTARDRPHAPLQRAGHGDLFETADGETYIAYLCGRPLPGRGRCVLGRETA 294 Query: 271 --PLTW-ENNWPVIGDGGKAPLSFETKLP--AGEPKPLV-ISDEFEYTENRLALQWQWNH 324 LTW + WP DG P + ET +P P + ++F+ T + QW Sbjct: 295 IQKLTWGADGWPRTLDGSGDP-TLETPGAGLVDQPWPTTPVREDFDGTTLPIDFQWLRTP 353 Query: 325 NPDNGLWSVTERPGYLRLRTGRIADDVLLARNTLTQRTEGPASSAETMLHVSGLSAGDRA 384 P+ ++S+T RPG+LRL GR + + + + +R + SA T L A Sbjct: 354 YPET-IFSLTARPGFLRL-YGRESLGSVFTQALVARRQQAHCYSAATRLDFEPAHFQQAA 411 Query: 385 GMVALQNG------YGTVEVAAGEQGQLDVNMCI-NGDKGTEVVESVPFTGERIGLRIDF 437 G+V NG + T + AG+ L V C + + +P + LR++ Sbjct: 412 GLVCYYNGSKLHYLHVTHDDEAGK--HLRVMTCTPDSPQADSFTAPIPLGAGPVDLRVEV 469 Query: 438 NFEDGVDEARFFYSGDGEVWHPIGQTLHMKYTLDHFMGYXXXXFN-----YATRQPGGY- 491 +FE RF + G W + + D F A + G Sbjct: 470 DFE----RLRFGFRQAGGDWRWLPEVFDASILSDEATAPGAPNFTGAFVGMACQDMSGMA 525 Query: 492 --ADFDYFRYHREE 503 ADFD+F Y + Sbjct: 526 TAADFDWFDYQERD 539 >BS_xynB [G] COG3507 Beta-xylosidase Length = 533 Score = 94.7 bits (234), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 133/555 (23%), Positives = 214/555 (38%), Gaps = 80/555 (14%) Query: 5 KITNPVIWADVPDVDVIRVGPVFYMVSTSMHSMPGCPIMKSVNLKDWEIVGYVYDTFEEN 64 KITNPV+ PD + R G +Y+ ++ PG I S +L +W +V + Sbjct: 2 KITNPVLKGFNPDPSICRAGEDYYIAVSTFEWFPGVQIHHSKDLVNWHLVAHPL-----Q 56 Query: 65 EAHRLEDGEGIYGSGSWAASLRHKNGTFYVCFSS--------NDMDQFYIYQTRDIEHGP 116 +L+ G WA L + +G F++ ++ D + + T + +G Sbjct: 57 RVSQLDMKGNPNSGGVWAPCLSYSDGKFWLIYTDVKVVDGAWKDCHNYLV--TCETINGD 114 Query: 117 WERSV-IPGLYHDPALLLDDDGRNYVI----------YGNGDIRVQELTADLTAVKPGGT 165 W + + D +L D DG+ Y++ + G I +QE + K G Sbjct: 115 WSEPIKLNSSGFDASLFHDTDGKKYLLNMLWDHRIDRHSFGGIVIQEYSD--KEQKLIGK 172 Query: 166 DQLLLEGERKDIMLRIEGCHAHKRDGYYFLFFIEWPSAGNQRRRQLCYRSRELLGPYE-- 223 +++ EG + + E H + YY+L E RS + GPYE Sbjct: 173 PKVIFEGTDRKLT---EAPHLYHIGNYYYLLTAE--GGTRYEHAATIARSANIEGPYEVH 227 Query: 224 -HRVVLDD--DLGYRNNGVAQGGIVDTPDGYWYAV-------------LFQDHG--AVGR 265 +L D G IV T WY +FQ G +GR Sbjct: 228 PDNPILTSWHDPGNPLQKCGHASIVQTHTDEWYLAHLTGRPIHPDDDSIFQQRGYCPLGR 287 Query: 266 IPCVFPLTWENNWPVIGDGGK------APLSFETKLPAGEPKPLVISDEFEYTENRLALQ 319 + L W++ WP + G + AP ET A P+ DEFE ++ L + Sbjct: 288 ETAIQKLYWKDEWPYVVGGKEGSLEVDAPSIPETIFEATYPE----VDEFE--DSTLNIN 341 Query: 320 WQWNHNP-DNGLWSVTERPGYLRLRTGRIADDVLLARNTLTQRTEGPASSAETMLHVSGL 378 +Q P N L S+T+ P +LRL G + + + +R + AET + Sbjct: 342 FQTLRIPFTNELGSLTQAPNHLRL-FGHESLTSTFTQAFVARRWQSLHFEAETAVEFYPE 400 Query: 379 SAGDRAGMVALQN--GYGTVEVAAGEQ--GQLDVNMCINGDKGTEVVESVPFTGERIGLR 434 + AG+V N + ++V E+ L++ +C N + + E + Sbjct: 401 NFQQAAGLVNYYNTENWTALQVTHDEELGRILELTICDNFSFSQPLNNKIVIPREVKYVY 460 Query: 435 IDFNFEDGVDEARFFYSGDGEVWHPIGQTLHMKYTLDHFM---GYXXXXFNYATRQPGG- 490 + N E D+ +FYS + E WH I L K D ++ G+ F Q G Sbjct: 461 LRVNIEK--DKYYYFYSFNKEDWHKIDIALESKKLSDDYIRGGGFFTGAFVGMQCQDTGG 518 Query: 491 ---YADFDYFRYHRE 502 ADF YFRY + Sbjct: 519 NHIPADFRYFRYKEK 533 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.140 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 43,147,394 Number of extensions: 1993737 Number of successful extensions: 3460 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 3434 Number of HSP's successfully gapped: 9 Length of query: 503 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 392 Effective length of database: 25,576,357 Effective search space: 10025931944 Effective search space used: 10025931944 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)