BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0006.p (573 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfa... 444 e-124 lin0980 [GEPR] COG0477 Permeases of the major facilitator superf... 265 1e-70 DR0258 [GEPR] COG0477 Permeases of the major facilitator superfa... 233 7e-61 PA0246 [GEPR] COG0477 Permeases of the major facilitator superfa... 231 3e-60 CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator supe... 189 1e-47 >BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 426 Score = 444 bits (1143), Expect = e-124, Method: Compositional matrix adjust. Identities = 230/407 (56%), Positives = 292/407 (71%), Gaps = 9/407 (2%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 MSAMDNTIVA+AMG IV++LG DK WVT++YMV VMAG PI+GKLSDMYGRKRF Sbjct: 24 MSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFSCLD 83 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 L L GSALCG A ++ QL I+RAIQGIGGGAL+PIAFTI+FD++PPEKRGK++G+FGA Sbjct: 84 LFFSLIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGA 143 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKESVTHSKQRIDWGGA 267 VFG+SSV+GPLLGA ITD + W WVFYIN+PIG ++ + YKES+ H KQ+IDWGGA Sbjct: 144 VFGLSSVLGPLLGAIITDSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGA 203 Query: 268 FTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPE-AVISFSMF 326 TLV +++CLMFALELGG Y W+S QI+GLF FAV I F +E+ A + A S Sbjct: 204 ITLVVSIVCLMFALELGGKTYDWNSIQIIGLFIVFAVFFIAFFIVEKKAEDPARYSARSS 263 Query: 327 RNRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTGG 386 R ILA Y FI+ V+IP +VQ VY SA ++G +L PMMIGSVI S GG Sbjct: 264 RMACLRQRQILAFLYEGIFIILAVFIPTFVQPVYASSAASAGFILTPMMIGSVIRSMIGG 323 Query: 387 LLTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMSS 446 + TK SFRN+ML+S + F G+ LS + PDT+R+ L +FM ++GFGVGF+FS+LS +S Sbjct: 324 IFQTKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTVFMMISGFGVGFNFSLLSAAS 383 Query: 447 IHHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAF 493 ++ + R RG+A ST++F+RS GMTLG + F NG + Sbjct: 384 MNDLEPRFRGTANSTNSFLRSFGMTLGC--------HHFRNGCKQTY 422 >lin0980 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 491 Score = 265 bits (677), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 151/454 (33%), Positives = 255/454 (56%), Gaps = 4/454 (0%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 M+A++ TIV++AM TIVS+L G++ + W+ S Y++ PI+GKLSD+YGRK F+ Sbjct: 19 MAAIEGTIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGRKNIFVIA 78 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 I+F+ GS+LCG A ++ QL I+RAIQGIG G ++P TI+ D+YP EKR K+ G G+ Sbjct: 79 TIIFIIGSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAKVLGFMGS 138 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKESVTHSKQRIDWGGA 267 +GI+ V GPL+G ++ D + W W+F+IN+PIG++ +L+ L +E V K ID+ GA Sbjct: 139 AWGIAGVFGPLVGGFLVDQLSWHWIFFINVPIGLLTILLILLYLREEVERVKLPIDYLGA 198 Query: 268 FTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFSMFR 327 A++ L+FAL+ G W ++ LF +L + F F+E+ A + ++ F +F+ Sbjct: 199 SLFTVALLGLLFALQRAGESLNWTEPLVVILFAVSILLFVAFYFVEKRAKDPIMPFVLFK 258 Query: 328 NRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTGGL 387 N + +++ + I VYIP++ QG+ G AT +G +L P+ + +IGS GG Sbjct: 259 NPVVLIGNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWIIGSFIGGK 318 Query: 388 LTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMSSI 447 L R+ + + + A L+ T+ + + A GFG G F+ +++ Sbjct: 319 LLMTLGNRHTVGIGVLITAASGLILALFPASTNDIFFYLNSAFMGFGFGIIFTTTTVTVQ 378 Query: 448 HHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAFGAGADTSSFGDPRA 507 Q G AT+++T R++G T+G+ +FG I + T A G+ + S+ Sbjct: 379 DAVPRFQTGIATASNTLFRTVGQTIGVAVFGTIFNSVLTTQFRAAAGSEVNRSNLNQ--- 435 Query: 508 ALTPEARAQIPAPVLEKITNALSGSIAHTFLWAL 541 ++P+ + ++E + + L + H W + Sbjct: 436 LISPQTSGNVNPDLVEPLRDILYSGL-HMVFWVM 468 >DR0258 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 544 Score = 233 bits (593), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 163/501 (32%), Positives = 259/501 (51%), Gaps = 22/501 (4%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 +S+++ T+V SAM ++S+LGG W +AY + PI G LSD YGR+ + G Sbjct: 33 LSSLNMTVVGSAMPRVISDLGGFHLYAWAFTAYSLTTTVTIPIVGTLSDRYGRRPLILLG 92 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 ++VF GS G S+ QL RAIQGIGGG LM ++FT + D++ P +RG+ G GA Sbjct: 93 IVVFALGSMGLGFVQSMEQLIALRAIQGIGGGTLMAMSFTAIADLFTPIERGRYQGYTGA 152 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYK---ESVTHSKQRIDW 264 V+GISSVVGPL+G ++TD+ GW+ VF++NLP ++A + ++ + K D Sbjct: 153 VWGISSVVGPLVGGFLTDHFGWRSVFFLNLPFALLALFFIWRFFRLPAPTPGSVKGGFDA 212 Query: 265 GGAFTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFS 324 GA L GAV L A+ GG YAWDS +I+GL G L + A++ Sbjct: 213 LGATLLAGAVTTLTLAVSWGGGTYAWDSPRILGLLGATLALGAWYAAHSARQERAILDLG 272 Query: 325 MFRNRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQT 384 + R+R +S+ + A +Y+P+Y+QGV G SA+ SGL L P+M G ++ S Sbjct: 273 LLRDRGILLASLAGFLVSAGMYAAILYLPLYMQGVRGSSASGSGLALAPLMGGMILTSTL 332 Query: 385 GGLLTTKTS-FRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLS 443 G L ++T ++ +++ A+ + STL+ T + M L G G+G S L+ Sbjct: 333 SGQLVSRTGRYKRLIVGGALVASLALVLSSTLSLSTPLWLAVTMMILLGLGLGPVNSQLT 392 Query: 444 MSSIHHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAFGAG------- 496 ++ + ++ GSAT + F R +G TL +++FG + ++ LS A Sbjct: 393 LAVQNAAPRQKLGSATGGNQFFRQIGGTLAVSLFGALVNAHLSHDLSAKLPAAVRRLPAP 452 Query: 497 -----ADTSSFGDPRA------ALTPEARAQIPAPVLEKITNALSGSIAHTFLWALVPAV 545 A+ + RA AL ++ AP+++ + + G+I FL + Sbjct: 453 MQDAIANPNLLTSERATHLLGWALKKMGHHEMLAPIMQALRGVMVGAIDQVFLVSAGLVG 512 Query: 546 LGFVFVLLMPGDRLTVRSKKQ 566 L FV L +P L R++ + Sbjct: 513 LAFVLTLALPERPLQGRAQSR 533 >PA0246 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 501 Score = 231 bits (589), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 163/493 (33%), Positives = 257/493 (52%), Gaps = 23/493 (4%) Query: 88 MSAMDNTIVASAMGTIVSELGGLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIFG 147 +SA+D TIVA ++ I ++ LD + WV SAYMV + PI+GKL D+YGR+R +F Sbjct: 24 LSALDQTIVAVSLPAISADYADLDLLAWVISAYMVAMTVSMPIYGKLGDLYGRRRLMLFA 83 Query: 148 LIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFGA 207 ++VF A S LCG A S+ QL + R +QGIG G LM ++ I+ DI PP +RG+ G F + Sbjct: 84 IVVFTAASLLCGLAQSMGQLVLARVLQGIGAGGLMAVSQAIIGDIVPPRERGRYQGYFSS 143 Query: 208 VFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKE-SVTHSKQRIDWGG 266 ++ I+SV GP+LG +T+Y+ W WVF+INLPIG++A + + V + ID+ G Sbjct: 144 MYAIASVAGPVLGGLLTEYLSWHWVFWINLPIGLLALAISRRTLVGLPVPRRQPVIDYLG 203 Query: 267 AFTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFSMF 326 +V + L+ A+ G AW ++GLF A+ L++F+ ER E ++ +F Sbjct: 204 IVLMVVGLTALLLAITQIGQGAAWSDPALLGLFALAALALLLFVAQERRFREPLVPMQLF 263 Query: 327 RNRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTGG 386 R S ++A F I TV +P+ Q V G A ++ L L+P+++G +G+ G Sbjct: 264 AVRAATLSWLVAFFASFQAISLTVLVPLRYQSVTGAGADSAALHLLPLVMGLPLGAYCCG 323 Query: 387 LLTTKT-SFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMS 445 LT ++ ++LL AV G+ L+ L P + + M LTG +G F ++ Sbjct: 324 RLTASLGRYKPLILLGAVLLPCGIAGLA-LVPVGDHWLSGLCMLLTGIAIGTQFPTSLVA 382 Query: 446 SIHHFDMRQRGSATSTSTFMRSLGMTLGIT-----IFGIIQRNSFTNGLSDAFGAGADTS 500 + + + R G ATS +T RSLG +G+ + +++ GL F A S Sbjct: 383 AQNAVEQRHIGVATSNATLFRSLGGAVGVALMSAILLALLKHLRIGGGLDQGFSESALLS 442 Query: 501 SFGDPRAALTPEARAQIPAPVLEKITNALSGSIAHTFLWALVPAVLGFVFVLLMPGDRLT 560 S A PA V+ + + L+ + FL A+LG +P +T Sbjct: 443 SLDA----------ASGPARVI--LQHVLADAFKVLFLVNAAVALLGLAVASALP--DMT 488 Query: 561 VRSK-KQQPADGQ 572 +R + QP G+ Sbjct: 489 LRGRGATQPTAGE 501 >CAC0020_1 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 458 Score = 189 bits (480), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 129/439 (29%), Positives = 214/439 (48%), Gaps = 7/439 (1%) Query: 88 MSAMDNTIVASAMGTIVSELG-GLDKIVWVTSAYMVMVMAGTPIFGKLSDMYGRKRFFIF 146 + +D+TIV A+ I L+ W+++ Y + + K +D +GRK+ I Sbjct: 19 LGVIDSTIVNIALPNITDYFKVSLNDTSWISTIYALALAVFMITAAKFADQFGRKKLMII 78 Query: 147 GLIVFLAGSALCGTATSITQLSIYRAIQGIGGGALMPIAFTIMFDIYPPEKRGKLTGLFG 206 G+++F SALCG + S+ L I R +QGIGG + PIA + +I+ E+ + G G Sbjct: 79 GILIFGVSSALCGISNSLLFLIIMRFVQGIGGAIITPIALPMGIEIFGKERMNIVVGASG 138 Query: 207 AVFGISSVVGPLLGAYITDYVGWQWVFYINLPIGVIAFILVTLSYKESVTHS-KQRIDWG 265 AV G+++ GP LG + Y+ WQ VF++N+P + A IL+ + ES ++ + IDW Sbjct: 139 AVVGLAAASGPPLGGVLIKYINWQSVFFVNVPFAIAALILIVICVNESYDNTVSKDIDWF 198 Query: 266 GAFTLVGAVICLMFALELGGNQYAWDSAQIMGLFGGFAVLLIVFLFIERVAPEAVISFSM 325 G TL ++ CL+FAL L G Y W S I+ LF F V LI+F+ IE +I ++ Sbjct: 199 GVMTLTISLFCLIFAL-LKGKDYGWTSTLIISLFLIFVVSLILFIIIELKVSSPMIEINL 257 Query: 326 FRNRLFATSSILALFYGSAFIVATVYIPIYVQGVYGGSATNSGLVLMPMMIGSVIGSQTG 385 F+ F SSI + G + + + Y+Q V + ++M + + + + G Sbjct: 258 FKEPTFTASSICYMITGFGLMCPLLILNYYLQNVLQYDTLEAAFIMMSVSLTGMFSTPIG 317 Query: 386 GLLTTKTSFRNIMLLSAVCFVAGVFSLSTLAPDTSRLMLNMFMALTGFGVGFSFSVLSMS 445 +L K S R I + + G+F LS L+ S+ + + + G G+GFS LS S Sbjct: 318 SILGKKVSPRIINFIGMLSLGIGIFRLSYLSYGMSKGTMIFDLIICGIGLGFSVQALS-S 376 Query: 446 SIHHFDMRQRGSATSTSTFMRSLGMTLGITIFGIIQRNSFTNGLSDAFGAGADTSSFGDP 505 S+ + G + R +G +GI I + NS + D D S+ + Sbjct: 377 SVKFIPKEKSGIGSGIVNAARQIGSCIGIAIL-VSMLNSNVSDAKD--NIKKDVVSYINN 433 Query: 506 RAALTPEARAQIPAPVLEK 524 + + R ++ V +K Sbjct: 434 KKEIIYPVRKELVKIVNDK 452 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,942,801 Number of extensions: 1400219 Number of successful extensions: 7290 Number of sequences better than 1.0e-05: 165 Number of HSP's gapped: 7156 Number of HSP's successfully gapped: 171 Length of query: 573 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 461 Effective length of database: 25,461,023 Effective search space: 11737531603 Effective search space used: 11737531603 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 118 (50.1 bits)