BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0204.0007.p (534 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0567 [E] COG0747 ABC-type dipeptide transport system, periplas... 256 6e-68 FN1504 [E] COG0747 ABC-type dipeptide transport system, periplas... 243 5e-64 ZnikA [E] COG0747 ABC-type dipeptide transport system, periplasm... 243 7e-64 nikA [E] COG0747 ABC-type dipeptide transport system, periplasmi... 243 7e-64 ECs4343 [E] COG0747 ABC-type dipeptide transport system, peripla... 243 8e-64 >BH0567 [E] COG0747 ABC-type dipeptide transport system, periplasmic component Length = 539 Score = 256 bits (654), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 159/520 (30%), Positives = 264/520 (50%), Gaps = 12/520 (2%) Query: 1 MIILAAFIMIAGCXXXXXXXXXXDATGQPPGGQEKAVTVAISADPGIDQLDAGAYK-GAM 59 +I+L +I+ + + EK++T + D G ++ Y + Sbjct: 8 LILLFVISLISSILVGCAESESGTVSNEGEENTEKSITFSWPRDIG--PMNPHVYNPSQL 65 Query: 60 SVHAMIYDGLVEYGEKGSILPALAESWDISEDGKVYTFHLRHGVKFSDGTDFNAAAVKFS 119 +MIY+ LV Y E G + P LA+SW ISEDGK YTF LR GV+FSDGT FNA VK + Sbjct: 66 FAQSMIYEPLVSYTEGGELQPHLADSWTISEDGKEYTFKLREGVQFSDGTPFNAEIVKKN 125 Query: 120 FERWMNDPA--NSLNVAAALQSIDVIDDHTLTMTFNKTYYPFLTELSFARPVRIISPSAV 177 F+ W+ + + L V L+ +V+D+ T M + YYP L +L+ RPVR + + Sbjct: 126 FDTWIEHSSLHSWLGVMNVLEKTEVVDEFTFKMVLKEPYYPALQDLAVVRPVRFLGEAGF 185 Query: 178 EPAGAVTGKFVKAVGTGAWMAESYKTDQEAVLVRNPYYWGEAPKLSKIILKVIPDPQSRV 237 G + + +GTG WM YK D+ AV RNP YWGE+PK+ K+ +K+IPD ++RV Sbjct: 186 PDDGDTSQGIKEPIGTGPWMLSDYKQDEYAVFTRNPNYWGESPKIDKVTVKIIPDAETRV 245 Query: 238 LALQSGAADIAGGQPGKLPVESVPVIESDSSLSLQKAPGTNSHFLIFNYNTPALQDLNVR 297 LA +SG D+ G+ G + +++ ++ + + L+ N + L DL VR Sbjct: 246 LAFESGELDLIFGE-GVISMDAFNQLKESGQYGTDLSEPVGTRSLLLNTSNEKLADLRVR 304 Query: 298 KAMNLAINKKSIIDDLLDGIGSEAKGLFPLTVPYVTDQNNTWYGFDPDEAKQLLAEAGYS 357 A++ NK+++++ + G+ +A + PY TD + +D ++A L EAG+ Sbjct: 305 LALHHGFNKQAMVEGVTLGLEEKADNILSTNFPY-TDIDVEPIEYDVEQANAYLDEAGWE 363 Query: 358 DSNGDGIVEKDGAALEFSFVLQQAEFPEWKSVSELIQSELKAIGIAVNLQILEPNAYYDA 417 G + EK+G LE + + + P K+++E +Q+E AIG+ +++ LE Sbjct: 364 LPAGKTVREKNGEQLELELIYDKTD-PLQKAMAETMQAEWAAIGVKLDITGLELTTQIQR 422 Query: 418 LWTTKAYDLIMYRTYDDAYNPHAFLLSLFHK---TAEAPAVAWSDAELESFIDTAVGSTD 474 +D+ + Y Y+PH+F+ + AEA + EL+ + + STD Sbjct: 423 R-RAGDFDVDFWYNYGAPYDPHSFINVVAEAGWGVAEAHSNLSMKEELDEQVRATLASTD 481 Query: 475 LLVRQKAYDSIFGRLYEEAMFGALYFPDEVMAVNKRVKGF 514 RQ+ Y SI L E+++F + + + + + V F Sbjct: 482 ETERQELYGSILNTLQEQSVFVPISYIKKTVVYQENVNEF 521 >FN1504 [E] COG0747 ABC-type dipeptide transport system, periplasmic component Length = 522 Score = 243 bits (620), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 154/493 (31%), Positives = 269/493 (54%), Gaps = 19/493 (3%) Query: 33 QEKAVTVAISADPGIDQLDAGAY-KGAMSVHAMIYDGLVEYGEKGSILPALAESWDISED 91 Q+K +T++ + D G L+ AY M+Y+GLV YGE G ILP+LAESW+ISED Sbjct: 23 QKKTLTISWNQDVGF--LNPHAYLPDQFITQGMVYEGLVNYGENGEILPSLAESWEISED 80 Query: 92 GKVYTFHLRHGVKFSDGTDFNAAAVKFSFER-WMNDPANS-LNVAAALQSIDVIDDHTLT 149 GK YTFHLR GVKFSDG+DFNA VK +F+ ++N +S + + S V++++T Sbjct: 81 GKTYTFHLRKGVKFSDGSDFNANNVKKNFDSIFLNKERHSWFGLTGHISSYRVVNENTFE 140 Query: 150 MTFNKTYYPFLTELSFARPVRIISPSAVEPAGAVTGKFVKA-VGTGAWMAESYKTDQEAV 208 ++ Y P L +L+ RP+R ++ + P T K +KA +GTG W+ + +K D+ A+ Sbjct: 141 FVLDEAYTPTLYDLAMIRPIRFLADAGF-PDDGDTYKGIKASIGTGPWILKEHKKDEYAI 199 Query: 209 LVRNPYYWGEAPKLSKIILKVIPDPQSRVLALQSGAADIAGGQPGKLPVESVPVIESDSS 268 +NP YWG+ P L ++++K+IPD ++R L ++G D+ G G + ++ + DS Sbjct: 200 FEKNPNYWGKKPILDEVVIKIIPDAETRALQFEAGELDMIYGN-GLISYDTFKSYQGDSK 258 Query: 269 LSLQKAPGTNSHFLIFNYNTPALQDLNVRKAMNLAINKKSIIDDLLDGIGSEAKGLFPLT 328 + ++ L+FN T L D+N+R A+ A +KK+I + +L+GI A +F Sbjct: 259 YKTDISEPMSTRLLMFNTTTGPLGDINLRYALTYATDKKAISEGILNGIEKPADTIFAPN 318 Query: 329 VPYVTDQNNTWYGFDPDEAKQLLAEAGYSDSNGDGIVEKDGAALEFSFVLQQAEFPEWKS 388 +P+ + Q+ + ++ D+AK+ + +AGY G EKDG L F + + K Sbjct: 319 MPH-SKQDLKPFEYNLDKAKEYIEKAGY--KMGKEFYEKDGKVLTLVFPYIATKTLD-KQ 374 Query: 389 VSELIQSELKAIGIAVNLQILEPNAYYDALWTTKAYDLIMYRTYDDAYNPHAFLLSLF-- 446 ++E IQ + K IG+ V ++ LE +++ K Y++++ ++ ++PHA++ ++ Sbjct: 375 IAEYIQGQWKKIGVNVEIKALEEKNFWEETDDLK-YNVMLNYSWGAPWDPHAYINAMATV 433 Query: 447 ----HKTAEAPAVAWSDAELESFIDTAVGSTDLLVRQKAYDSIFGRLYEEAMFGALYFPD 502 + EA EL++ I + + ++ Y I L+E+A++ L + Sbjct: 434 AENGNPDYEAQLGLPMKKELDAKIHQVLLEANPEKVEQLYKEILTTLHEQAVYVPLTYQS 493 Query: 503 EVMAVNKRVKGFK 515 + + G + Sbjct: 494 LIAVYRDNLTGVR 506 >ZnikA [E] COG0747 ABC-type dipeptide transport system, periplasmic component Length = 524 Score = 243 bits (619), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 158/445 (35%), Positives = 238/445 (53%), Gaps = 15/445 (3%) Query: 59 MSVHAMIYDGLVEYGEKGSILPALAESWDISEDGKVYTFHLRHGVKFSDGTDFNAAAVKF 118 M +M+Y+ LV+Y GS++P LA+SW SEDGK +TF LR VKFS+G F+A A Sbjct: 49 MFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFDAEAAAE 108 Query: 119 SFERWMNDPANSLNVAAALQSIDV--IDDHTLTMTFNKTYYPFLTELSFARPVRIISPSA 176 +F +++ + A Q +DV + L +T YYPFL EL+ RP R I+PS Sbjct: 109 NFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRPFRFIAPSQ 168 Query: 177 VEPAGAVTGKFVKA-VGTGAWMAESYKTDQEAVLVRNPYYWGEAPKLSKIILKVIPDPQS 235 + + G +KA +GTG W+ + K +Q V VRN YWGE P + KI VIPDP + Sbjct: 169 FKNHETMNG--IKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITFNVIPDPTT 226 Query: 236 RVLALQSGAADIAGGQPGKLPVESVPVIESDSSLSLQKAPGTNSHFLIFNYNTPALQDLN 295 R +A ++G D+ G G LP+++ + + Q + + L N +L Sbjct: 227 RAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLAXNTAKAPTNELA 286 Query: 296 VRKAMNLAINKKSIIDDLLDGIGSEAKGLFPLTVPYVTDQNNTWYGFDPDEAKQLLAEAG 355 VR+A+N A+NKKS+ID+ L G A LF +VPY + +DP +AK LL +AG Sbjct: 287 VREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYA-NLGLKPRQYDPQKAKALLEKAG 345 Query: 356 YSDSNGDGIVEKDGAAL--EFSFVLQQAEFPEWKSVSELIQSELKAIGIAVNLQILEPNA 413 ++ G I EK+G L E SF+ A KS++E+IQ++++ IG V+L E ++ Sbjct: 346 WTLPAGKDIREKNGQPLHIELSFIGTDALS---KSMAEIIQADMRQIGADVSLIGEEESS 402 Query: 414 YYDALWTTKAYDLIMYRTYDDAYNPHAFLLSLF---HKTAEAPAVAWSDAELESFIDTAV 470 Y A + +I +RT+ Y+PHAFL S+ H +A ++ I + Sbjct: 403 IY-ARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADKPLIDKEIGEVL 461 Query: 471 GSTDLLVRQKAYDSIFGRLYEEAMF 495 + D RQ Y I RL++EA++ Sbjct: 462 ATHDEAQRQALYRDILTRLHDEAVY 486 >nikA [E] COG0747 ABC-type dipeptide transport system, periplasmic component Length = 524 Score = 243 bits (619), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 158/445 (35%), Positives = 238/445 (53%), Gaps = 15/445 (3%) Query: 59 MSVHAMIYDGLVEYGEKGSILPALAESWDISEDGKVYTFHLRHGVKFSDGTDFNAAAVKF 118 M +M+Y+ LV+Y GS++P LA+SW SEDGK +TF LR VKFS+G F+A A Sbjct: 49 MFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFDAEAAAE 108 Query: 119 SFERWMNDPANSLNVAAALQSIDV--IDDHTLTMTFNKTYYPFLTELSFARPVRIISPSA 176 +F +++ + A Q +DV + L +T YYPFL EL+ RP R I+PS Sbjct: 109 NFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRPFRFIAPSQ 168 Query: 177 VEPAGAVTGKFVKA-VGTGAWMAESYKTDQEAVLVRNPYYWGEAPKLSKIILKVIPDPQS 235 + + G +KA +GTG W+ + K +Q V VRN YWGE P + KI VIPDP + Sbjct: 169 FKNHETMNG--IKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITFNVIPDPTT 226 Query: 236 RVLALQSGAADIAGGQPGKLPVESVPVIESDSSLSLQKAPGTNSHFLIFNYNTPALQDLN 295 R +A ++G D+ G G LP+++ + + Q + + L N +L Sbjct: 227 RAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALNTAKAPTNELA 286 Query: 296 VRKAMNLAINKKSIIDDLLDGIGSEAKGLFPLTVPYVTDQNNTWYGFDPDEAKQLLAEAG 355 VR+A+N A+NKKS+ID+ L G A LF +VPY + +DP +AK LL +AG Sbjct: 287 VREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYA-NLGLKPSQYDPQKAKALLEKAG 345 Query: 356 YSDSNGDGIVEKDGAAL--EFSFVLQQAEFPEWKSVSELIQSELKAIGIAVNLQILEPNA 413 ++ G I EK+G L E SF+ A KS++E+IQ++++ IG V+L E ++ Sbjct: 346 WTLPAGKDIREKNGQPLRIELSFIGTDALS---KSMAEIIQADMRQIGADVSLIGEEESS 402 Query: 414 YYDALWTTKAYDLIMYRTYDDAYNPHAFLLSLF---HKTAEAPAVAWSDAELESFIDTAV 470 Y A + +I +RT+ Y+PHAFL S+ H +A ++ I + Sbjct: 403 IY-ARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADKPLIDKEIGEVL 461 Query: 471 GSTDLLVRQKAYDSIFGRLYEEAMF 495 + D RQ Y I RL++EA++ Sbjct: 462 ATHDETQRQALYRDILTRLHDEAVY 486 >ECs4343 [E] COG0747 ABC-type dipeptide transport system, periplasmic component Length = 524 Score = 243 bits (619), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 158/445 (35%), Positives = 238/445 (53%), Gaps = 15/445 (3%) Query: 59 MSVHAMIYDGLVEYGEKGSILPALAESWDISEDGKVYTFHLRHGVKFSDGTDFNAAAVKF 118 M +M+Y+ LV+Y GS++P LA+SW SEDGK +TF LR VKFS+G F+A A Sbjct: 49 MFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFDAEAAAE 108 Query: 119 SFERWMNDPANSLNVAAALQSIDV--IDDHTLTMTFNKTYYPFLTELSFARPVRIISPSA 176 +F +++ + A Q +DV + L +T YYPFL EL+ RP R I+PS Sbjct: 109 NFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRPFRFIAPSQ 168 Query: 177 VEPAGAVTGKFVKA-VGTGAWMAESYKTDQEAVLVRNPYYWGEAPKLSKIILKVIPDPQS 235 + + G +KA +GTG W+ + K +Q V VRN YWGE P + KI VIPDP + Sbjct: 169 FKNHETMNG--IKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITFNVIPDPTT 226 Query: 236 RVLALQSGAADIAGGQPGKLPVESVPVIESDSSLSLQKAPGTNSHFLIFNYNTPALQDLN 295 R +A ++G D+ G G LP+++ + + Q + + L N +L Sbjct: 227 RAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALNTAKAPTNELA 286 Query: 296 VRKAMNLAINKKSIIDDLLDGIGSEAKGLFPLTVPYVTDQNNTWYGFDPDEAKQLLAEAG 355 VR+A+N A+NKKS+ID+ L G A LF +VPY + +DP +AK LL +AG Sbjct: 287 VREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYA-NLGLKPRQYDPQKAKALLEKAG 345 Query: 356 YSDSNGDGIVEKDGAAL--EFSFVLQQAEFPEWKSVSELIQSELKAIGIAVNLQILEPNA 413 ++ G I EK+G L E SF+ A KS++E+IQ++++ IG V+L E ++ Sbjct: 346 WTLPAGKDIREKNGQPLHIELSFIGTDALS---KSMAEIIQADMRQIGADVSLIGEEESS 402 Query: 414 YYDALWTTKAYDLIMYRTYDDAYNPHAFLLSLF---HKTAEAPAVAWSDAELESFIDTAV 470 Y A + +I +RT+ Y+PHAFL S+ H +A ++ I + Sbjct: 403 IY-ARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADKPLIDKEIGEVL 461 Query: 471 GSTDLLVRQKAYDSIFGRLYEEAMF 495 + D RQ Y I RL++EA++ Sbjct: 462 ATHDEAQRQALYRDILTRLHDEAVY 486 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,630,893 Number of extensions: 1699358 Number of successful extensions: 4196 Number of sequences better than 1.0e-05: 198 Number of HSP's gapped: 3659 Number of HSP's successfully gapped: 209 Length of query: 534 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 423 Effective length of database: 25,576,357 Effective search space: 10818799011 Effective search space used: 10818799011 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)