BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0204.0006.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20059 [QR] COG0500 SAM-dependent methyltransferases 107 2e-23 MA0297 [QR] COG0500 SAM-dependent methyltransferases 105 5e-23 NMA2151 [EH] COG0115 Branched-chain amino acid aminotransferase/... 105 9e-23 MA0769 [QR] COG0500 SAM-dependent methyltransferases 101 1e-21 MA0303 [QR] COG0500 SAM-dependent methyltransferases 96 5e-20 >SMb20059 [QR] COG0500 SAM-dependent methyltransferases Length = 259 Score = 107 bits (266), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 55/149 (36%), Positives = 86/149 (57%), Gaps = 3/149 (2%) Query: 4 QEISKYWTSSSVGYDKVIQTQFRSRKTVRLWKQLLIQGMGLKSRQKVLDVGTGPGFFSIL 63 ++I +YW+ S +D + + W + +G + KVL++ G G + + Sbjct: 10 EDIREYWSKRSQTFDLAFGHRIPPGPELDAWAAAMRDALGARP-LKVLELACGTGEVTNV 68 Query: 64 LSQMGHQATAVDASLGMIERAARNFDRYGCKVQAYVGDAADLTAEPDNSFDAVVCRDMVW 123 L +GH+ TA+D S M+ A R G ++ A+ T EPD ++DAVVCR +VW Sbjct: 69 LLSLGHEVTALDFSEAMLAVARRK--HAGNDRVRFILADAERTMEPDETYDAVVCRHLVW 126 Query: 124 TLPDPQKAYAEWHRLLKPGGTLIVFDGNY 152 TL +P++A+AEW RLLKPGG L+VFDG++ Sbjct: 127 TLTEPEQAFAEWFRLLKPGGRLLVFDGDW 155 >MA0297 [QR] COG0500 SAM-dependent methyltransferases Length = 253 Score = 105 bits (263), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 81/244 (33%), Positives = 115/244 (47%), Gaps = 23/244 (9%) Query: 4 QEISKYWTSSSVGYDKV----IQTQFRSRKTVRLWKQLLIQGMGLKSRQKVLDVGTGPGF 59 Q I++ W SS YD IQ++ R++K LL G R KVLDVG G Sbjct: 10 QNIARIWDVSSETYDSHEGHGIQSEMEREAWKRIFKSLLPPG-----RLKVLDVGCGTCE 64 Query: 60 FSILLSQMGHQATAVDASLGMIERAARNFDRYGCKVQAYVGDAADLTAEPDNSFDAVVCR 119 +L ++MGHQ T +D S M+ +A R G GDA E D +FD VV R Sbjct: 65 IGLLFAEMGHQVTGLDLSEKMLAKAREKASRKGFDSVFEKGDAEAPPFEED-TFDVVVNR 123 Query: 120 DMVWTLPDPQKAYAEWHRLLKPGGTLIVFDGNYMYKENKSLFRRLWYALSWMLILITEKR 179 ++WTLP P A W LK GG LIV DG + ++ RR +S L+ E+R Sbjct: 124 HLLWTLPHPDTAVMNWRNALKKGGVLIVVDGVWNDGSFETKARRF---VSDFCTLLAERR 180 Query: 180 IRQRSSKGKSLLSELPFVNVLRPEADEQALLHAGFR---------IFDTRRNFIPARK-L 229 ++ + + SELP + + PE + AGF+ I + ++ +P RK + Sbjct: 181 NPRKGHYSREIDSELPNRHGVPPEKMLEYFRAAGFKNVKDLDLKDIMELQKKKMPYRKRI 240 Query: 230 PLNY 233 NY Sbjct: 241 AYNY 244 >NMA2151 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 261 Score = 105 bits (261), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 54/150 (36%), Positives = 88/150 (58%), Gaps = 4/150 (2%) Query: 4 QEISKYWTSSSVGYDKVIQTQFRSRKTVRLWKQLLIQGMGLKSRQKVLDVGTGPGFFSIL 63 + + KYW S YD + ++WK+LL +G + +LDVG+G G ++ Sbjct: 16 EGVKKYWDYGSKFYDTA--PGLGGNEESQIWKKLLSSSIG-PDLKNILDVGSGTGIIAMY 72 Query: 64 LSQMGHQATAVDASLGMIERAARNFDRYGCKVQAYVGDAADLTAEPDNSFDAVVCRDMVW 123 L+++G+ TAVD S GM++ A + G K++ GD +L+ E D +FD V R ++W Sbjct: 73 LAELGYGVTAVDFSEGMMDIARKKALEKGAKIRFMEGDIENLSFE-DETFDCVTARYVLW 131 Query: 124 TLPDPQKAYAEWHRLLKPGGTLIVFDGNYM 153 TLP P+KA EW R++KPGG +++ DG ++ Sbjct: 132 TLPHPEKALKEWVRVVKPGGRIVIIDGKWV 161 >MA0769 [QR] COG0500 SAM-dependent methyltransferases Length = 254 Score = 101 bits (251), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 68/218 (31%), Positives = 101/218 (46%), Gaps = 13/218 (5%) Query: 2 MLQEISKYWTSSSVGYDKV----IQTQFRSRKTVRLWKQLLIQGMGLKSRQKVLDVGTGP 57 M +I+ W SS YD IQ++ L++ LL G R +VLD G G Sbjct: 8 MKLDIAHVWDISSATYDNKEGHGIQSEIEKEAWKTLFRSLLPSG-----RLEVLDAGCGT 62 Query: 58 GFFSILLSQMGHQATAVDASLGMIERAARNFDRYGCKVQAYVGDAADLTAEPDNSFDAVV 117 G +L ++MGH T +D S M+ +A R + GDA + E + +FD VV Sbjct: 63 GEIGLLFTEMGHHVTGLDLSEQMLAKAREKTSRKKYDINFRAGDAENPPFEAE-TFDVVV 121 Query: 118 CRDMVWTLPDPQKAYAEWHRLLKPGGTLIVFDGNYMYKENKSLFRRLWYALSWMLILITE 177 R ++WTLP P A W ++L+ GG LIV DG Y S+ R+ +S L + E Sbjct: 122 TRHLLWTLPHPDTAVRNWEKVLRKGGVLIVIDGLY---NGGSIERKTRQFISDFLTFLVE 178 Query: 178 KRIRQRSSKGKSLLSELPFVNVLRPEADEQALLHAGFR 215 +R R + +ELP + P+ + GF+ Sbjct: 179 RRYPGRKQYPDEIKTELPNPYGVPPKKTIEYFRKTGFK 216 >MA0303 [QR] COG0500 SAM-dependent methyltransferases Length = 256 Score = 95.9 bits (237), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 68/223 (30%), Positives = 110/223 (49%), Gaps = 16/223 (7%) Query: 6 ISKYWTSSSVGYDKVIQTQFRSRKTVRLWKQL---LIQGMGLKSRQKVLDVGTGPGFFSI 62 ++ W +S +D +S+K WK + G GL+ +LDVG G G S+ Sbjct: 13 VANRWDLASETFDTHAGHGIQSQKERDEWKHAFRGIFPGKGLR----ILDVGCGTGELSL 68 Query: 63 LLSQMGHQATAVDASLGMIERAARNFDRYGCKVQAYVGDAADLTAEPDNSFDAVVCRDMV 122 L ++MGH+ T +D S M++ A + V GDA + EP +SFD V R ++ Sbjct: 69 LFAEMGHRVTGIDISRKMLKTAKTKAEACRANVTFGEGDAENPPFEP-SSFDIVFSRHLL 127 Query: 123 WTLPDPQKAYAEWHRLLKPGGTLIVFDGNYMYKENKSLFRRLWYALSWMLILITEKRIRQ 182 WTLP+P+KA W R+L+ G +++ DG + + +L R +L LI E++ + Sbjct: 128 WTLPNPKKAVENWGRVLRKNGKVVIVDGVW---DRGTLGDRARKNAGALLTLILERKDPR 184 Query: 183 RSSKGKS--LLSELPFVNVLRPEADEQALLHAGF---RIFDTR 220 + G S L + LP E ++ L+ +GF R FD + Sbjct: 185 KGHAGYSEELKARLPHAGGAPLEKVKEYLVASGFEEVRTFDLK 227 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,544,611 Number of extensions: 725883 Number of successful extensions: 5876 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 5921 Number of HSP's successfully gapped: 29 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 114 (48.5 bits)