BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0204.0001.p (481 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZaldA [C] COG1012 NAD-dependent aldehyde dehydrogenases 668 0.0 ECs2021 [C] COG1012 NAD-dependent aldehyde dehydrogenases 668 0.0 aldA [C] COG1012 NAD-dependent aldehyde dehydrogenases 666 0.0 ZaldA [C] COG1012 NAD-dependent aldehyde dehydrogenases 666 0.0 NMB1968 [C] COG1012 NAD-dependent aldehyde dehydrogenases 601 e-172 >ZaldA [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 479 Score = 668 bits (1723), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/473 (67%), Positives = 379/473 (80%) Query: 3 KHLMYIDGQFTESEGKEWMDVTNPATDEVISQVPKATRNDVVRAIHAANKAQTAWEETPA 62 +H MYIDGQF G W+DV NPAT+ VIS++P D +AI AA +AQ WE PA Sbjct: 6 QHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPA 65 Query: 63 VERGKYLHAIAGGIRAEADSLARLISEEVGKTLELSTVEVNFTADYMDYMAEWARRYEGE 122 +ER +L I+ GIR A ++ LI EE GK +L+ VEV FTADY+DYMAEWARRYEGE Sbjct: 66 IERASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGE 125 Query: 123 IVQSDRDNENIFVFKRAIGVTTGILPWNFPFFLIARKMAPALITGNTIVVKPSAESPNNA 182 I+QSDR ENI +FKRA+GVTTGILPWNFPFFLIARKMAPAL+TGNTIV+KPS +PNNA Sbjct: 126 IIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNA 185 Query: 183 VAFAQIVAKAGLPQGVFNLVTGKGGEVGNELASNPKVGMVSLTGSVPAGQKVMEAAAENI 242 +AFA+IV + GLP+GVFNLV G+G VG ELA NPKV MVS+TGSV AG+K+M AA+NI Sbjct: 186 IAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNI 245 Query: 243 IKVSLELGGKAPAIVMXXXXXXXXIKAIVDSRVINTGQVCNCAERVYVHEKIKDEFTSRL 302 KV LELGGKAPAIVM +KAIVDSRVIN+GQVCNCAERVYV + I D+F +RL Sbjct: 246 TKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRL 305 Query: 303 VEAMKAVKYGDPLKDTDIQMGPLINKAAQESVQQKVDRAVQEGAKIALGGKKVEGAGSFF 362 EAM+AV++G+P + DI MGPLIN AA E V+QKV RAV+EGA++ALGGK VEG G ++ Sbjct: 306 GEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVALGGKAVEGKGYYY 365 Query: 363 EPTVITGATNEMEIVQEEIFGPVIPVISFSNLDEAIALANDSEFGLTSSLYTRNLNVAMK 422 PT++ EM I+ EE FGPV+PV++F L++AI++ANDS++GLTSS+YT+NLNVAMK Sbjct: 366 PPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMK 425 Query: 423 VIKCLKYGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLNEYLQTHVVYLQ 475 IK LK+GETYINRENFEAMQGFHAGWRKSGIGGADGKHGL+EYLQT VVYLQ Sbjct: 426 AIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQ 478 >ECs2021 [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 479 Score = 668 bits (1723), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/473 (67%), Positives = 379/473 (80%) Query: 3 KHLMYIDGQFTESEGKEWMDVTNPATDEVISQVPKATRNDVVRAIHAANKAQTAWEETPA 62 +H MYIDGQF G W+DV NPAT+ VIS++P D +AI AA +AQ WE PA Sbjct: 6 QHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPA 65 Query: 63 VERGKYLHAIAGGIRAEADSLARLISEEVGKTLELSTVEVNFTADYMDYMAEWARRYEGE 122 +ER +L I+ GIR A ++ LI EE GK +L+ VEV FTADY+DYMAEWARRYEGE Sbjct: 66 IERASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGE 125 Query: 123 IVQSDRDNENIFVFKRAIGVTTGILPWNFPFFLIARKMAPALITGNTIVVKPSAESPNNA 182 I+QSDR ENI +FKRA+GVTTGILPWNFPFFLIARKMAPAL+TGNTIV+KPS +PNNA Sbjct: 126 IIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNA 185 Query: 183 VAFAQIVAKAGLPQGVFNLVTGKGGEVGNELASNPKVGMVSLTGSVPAGQKVMEAAAENI 242 +AFA+IV + GLP+GVFNLV G+G VG ELA NPKV MVS+TGSV AG+K+M AA+NI Sbjct: 186 IAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNI 245 Query: 243 IKVSLELGGKAPAIVMXXXXXXXXIKAIVDSRVINTGQVCNCAERVYVHEKIKDEFTSRL 302 KV LELGGKAPAIVM +KAIVDSRVIN+GQVCNCAERVYV + I D+F +RL Sbjct: 246 TKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRL 305 Query: 303 VEAMKAVKYGDPLKDTDIQMGPLINKAAQESVQQKVDRAVQEGAKIALGGKKVEGAGSFF 362 EAM+AV++G+P + DI MGPLIN AA E V+QKV RAV+EGA++ALGGK VEG G ++ Sbjct: 306 GEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVALGGKAVEGKGYYY 365 Query: 363 EPTVITGATNEMEIVQEEIFGPVIPVISFSNLDEAIALANDSEFGLTSSLYTRNLNVAMK 422 PT++ EM I+ EE FGPV+PV++F L++AI++ANDS++GLTSS+YT+NLNVAMK Sbjct: 366 PPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMK 425 Query: 423 VIKCLKYGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLNEYLQTHVVYLQ 475 IK LK+GETYINRENFEAMQGFHAGWRKSGIGGADGKHGL+EYLQT VVYLQ Sbjct: 426 AIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQ 478 >aldA [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 479 Score = 666 bits (1719), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/473 (66%), Positives = 378/473 (79%) Query: 3 KHLMYIDGQFTESEGKEWMDVTNPATDEVISQVPKATRNDVVRAIHAANKAQTAWEETPA 62 +H MYIDGQF G W+DV NPAT+ VIS++P D +AI AA +AQ WE PA Sbjct: 6 QHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPA 65 Query: 63 VERGKYLHAIAGGIRAEADSLARLISEEVGKTLELSTVEVNFTADYMDYMAEWARRYEGE 122 +ER +L I+ GIR A ++ LI EE GK +L+ VEV FTADY+DYMAEWARRYEGE Sbjct: 66 IERASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGE 125 Query: 123 IVQSDRDNENIFVFKRAIGVTTGILPWNFPFFLIARKMAPALITGNTIVVKPSAESPNNA 182 I+QSDR ENI +FKRA+GVTTGILPWNFPFFLIARKMAPAL+TGNTIV+KPS +PNNA Sbjct: 126 IIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNA 185 Query: 183 VAFAQIVAKAGLPQGVFNLVTGKGGEVGNELASNPKVGMVSLTGSVPAGQKVMEAAAENI 242 +AFA+IV + GLP+GVFNLV G+G VG ELA NPKV MVS+TGSV AG+K+M AA+NI Sbjct: 186 IAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNI 245 Query: 243 IKVSLELGGKAPAIVMXXXXXXXXIKAIVDSRVINTGQVCNCAERVYVHEKIKDEFTSRL 302 KV LELGGKAPAIVM +KAIVDSRVIN+GQVCNCAERVYV + I D+F +RL Sbjct: 246 TKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRL 305 Query: 303 VEAMKAVKYGDPLKDTDIQMGPLINKAAQESVQQKVDRAVQEGAKIALGGKKVEGAGSFF 362 EAM+AV++G+P + DI MGPLIN AA E V+QKV RAV+EGA++A GGK VEG G ++ Sbjct: 306 GEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEGKGYYY 365 Query: 363 EPTVITGATNEMEIVQEEIFGPVIPVISFSNLDEAIALANDSEFGLTSSLYTRNLNVAMK 422 PT++ EM I+ EE FGPV+PV++F L++AI++ANDS++GLTSS+YT+NLNVAMK Sbjct: 366 PPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMK 425 Query: 423 VIKCLKYGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLNEYLQTHVVYLQ 475 IK LK+GETYINRENFEAMQGFHAGWRKSGIGGADGKHGL+EYLQT VVYLQ Sbjct: 426 AIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQ 478 Score = 666 bits (1719), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/473 (67%), Positives = 379/473 (80%) Query: 3 KHLMYIDGQFTESEGKEWMDVTNPATDEVISQVPKATRNDVVRAIHAANKAQTAWEETPA 62 +H MYIDGQF G W+DV NPAT+ VIS++P D +AI AA +AQ WE PA Sbjct: 6 QHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPA 65 Query: 63 VERGKYLHAIAGGIRAEADSLARLISEEVGKTLELSTVEVNFTADYMDYMAEWARRYEGE 122 +ER +L I+ GIR A ++ LI EE GK +L+ VEV FTADY+DYMAEWARRYEGE Sbjct: 66 IERASWLRKISAGIRERASEISALIVEEGGKIQQLAEVEVAFTADYIDYMAEWARRYEGE 125 Query: 123 IVQSDRDNENIFVFKRAIGVTTGILPWNFPFFLIARKMAPALITGNTIVVKPSAESPNNA 182 I+QSDR ENI +FKRA+GVTTGILPWNFPFFLIARKMAPAL+TGNTIV+KPS +PNNA Sbjct: 126 IIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNA 185 Query: 183 VAFAQIVAKAGLPQGVFNLVTGKGGEVGNELASNPKVGMVSLTGSVPAGQKVMEAAAENI 242 +AFA+IV + GLP+GVFNLV G+G VG ELA NPKV MVS+TGSV AG+K+M AA+NI Sbjct: 186 IAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNI 245 Query: 243 IKVSLELGGKAPAIVMXXXXXXXXIKAIVDSRVINTGQVCNCAERVYVHEKIKDEFTSRL 302 KV LELGGKAPAIVM +KAIVDSRVIN+GQVCNCAERVYV + I D+F +RL Sbjct: 246 TKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFVNRL 305 Query: 303 VEAMKAVKYGDPLKDTDIQMGPLINKAAQESVQQKVDRAVQEGAKIALGGKKVEGAGSFF 362 EAM+AV++G+P + DI MGPLIN AA E V+QKV RAV+EGA++ALGGK VEG G ++ Sbjct: 306 GEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVALGGKAVEGKGYYY 365 Query: 363 EPTVITGATNEMEIVQEEIFGPVIPVISFSNLDEAIALANDSEFGLTSSLYTRNLNVAMK 422 PT++ EM I+ EE FGPV+PV++F L++AI++ANDS++GLTSS+YT+NLNVAMK Sbjct: 366 PPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNVAMK 425 Query: 423 VIKCLKYGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLNEYLQTHVVYLQ 475 IK LK+GETYINRENFEAMQGFHAGWRKSGIGGADGKHGL+EYLQT VVYLQ Sbjct: 426 AIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQ 478 >NMB1968 [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 480 Score = 601 bits (1550), Expect = e-172, Method: Compositional matrix adjust. Identities = 298/474 (62%), Positives = 361/474 (76%), Gaps = 2/474 (0%) Query: 6 MYIDGQFTESEGKEWMDVTNPATDEVISQVPKATRNDVVRAIHAANKAQTAWEETPAVER 65 MYI+G+F EW DV NP+T+E I++ PK + DV RA+ AA AQ AWE PAVER Sbjct: 6 MYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGKADVDRAVAAARAAQPAWERLPAVER 65 Query: 66 GKYLHAIAGGIRAEADSLARLISEEVGKTLELSTVEVNFTADYMDYMAEWARRYEGEIVQ 125 G YL IA GIR AD L I E GKT +L+ VEV FTADY+DY AEWARRYEGEI+Q Sbjct: 66 GAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEVMFTADYLDYQAEWARRYEGEIIQ 125 Query: 126 SDRDNENIFVFKRAIGVTTGILPWNFPFFLIARKMAPALITGNTIVVKPSAESPNNAVAF 185 SDR ENI +FKR +GV GILPWNFPFFLIARKM PAL+TGNTIVVKPS+ +P N F Sbjct: 126 SDRPRENILLFKRPLGVIAGILPWNFPFFLIARKMGPALVTGNTIVVKPSSVTPINCHIF 185 Query: 186 AQIVAKAGLPQGVFNLVTGKGGEVGNELASNPKVGMVSLTGSVPAGQKVMEAAAENIIKV 245 A+IV GLP GVFN+V G G E+GN L+++P+V MVSLTGSV AG++VMEAA+ NI KV Sbjct: 186 AEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQVDMVSLTGSVEAGRQVMEAASANITKV 245 Query: 246 SLELGGKAPAIVMXXXXXXXXIKAIVDSRVINTGQVCNCAERVYVHEKIKDEFTSRLVEA 305 SLELGGKAPAIV+ +K+I+ SRV NTGQ+CNCAERVYVH +KD F ++ A Sbjct: 246 SLELGGKAPAIVLKDADLDLAVKSILASRVGNTGQICNCAERVYVHSSLKDAFIEKMTAA 305 Query: 306 MKAVKYGDPLKDT--DIQMGPLINKAAQESVQQKVDRAVQEGAKIALGGKKVEGAGSFFE 363 MK V+YG+P + ++MGPLI + A ++V +KV+RAV++GAK+ GGK+ EG G FFE Sbjct: 306 MKGVRYGNPAEAEAGALEMGPLIEERAVKAVAEKVERAVKQGAKLVCGGKRAEGRGYFFE 365 Query: 364 PTVITGATNEMEIVQEEIFGPVIPVISFSNLDEAIALANDSEFGLTSSLYTRNLNVAMKV 423 PT++T N M+I++EE FGPV+PV +F LD+ IALAND EFGLTSS+YT NLN A V Sbjct: 366 PTLLTDTDNSMDIMKEETFGPVLPVSAFDTLDQVIALANDCEFGLTSSVYTTNLNEAFYV 425 Query: 424 IKCLKYGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLNEYLQTHVVYLQYD 477 + L++GETYINRENFEAMQGFHAGW+KSGIGGADGKHGL EYLQT VVYL+ D Sbjct: 426 TRRLQFGETYINRENFEAMQGFHAGWKKSGIGGADGKHGLEEYLQTQVVYLETD 479 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,083,832 Number of extensions: 1464326 Number of successful extensions: 5248 Number of sequences better than 1.0e-05: 209 Number of HSP's gapped: 4616 Number of HSP's successfully gapped: 210 Length of query: 481 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 371 Effective length of database: 25,691,691 Effective search space: 9531617361 Effective search space used: 9531617361 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)