BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0203.0004.p (460 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1697 [T] COG2200 FOG: EAL domain 278 1e-74 STM1080 [R] COG1092 Predicted SAM-dependent methyltransferases 276 7e-74 VC1354 [R] COG1092 Predicted SAM-dependent methyltransferases 270 3e-72 Z1319 [R] COG1092 Predicted SAM-dependent methyltransferases 270 4e-72 ECs1051 [R] COG1092 Predicted SAM-dependent methyltransferases 270 4e-72 >STM1697 [T] COG2200 FOG: EAL domain Length = 198 Score = 278 bits (711), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 0/452 (0%), Positives = 0/452 (0%), Gaps = 75/452 (16%) Query: 7 KRLEQGHPWVYASEVASVDGDPQPGGLVDVLNHQGRYLGTGYYNPASQIRVRILARNPLA 66 + +E V + + + + + I + Sbjct: 11 RAMEGDLLGVEITTHFASSPARPLHPEFVISSWDNSQKRRFLLDLLRTIAAKHGWFLR-H 69 Query: 67 AMDTAFFAGRLASCLRHRQRFLPGADAYRLVYGEADFLPGLIIDRFGEVLVVQLLTLGMD 126 + R + L + + + L D ++ L + Sbjct: 70 GLFCIVNIDRGMAQLVLQDKDIRALLHAMLFVELQVAEHFSCQDNALIDPLIHALHKQPN 129 Query: 127 KCRDQIVQALVEVVAPRGIYERSDVSVRELEGLEQRTGVLYGECPRHITVSENGLKVIVD 186 + AP S V + + Q + + +HI Sbjct: 130 PLWLGDLGVGNATAAPLVCGCFSGVKLDRSFFVSQIEKMTFPLLVKHIRRVIVTRSWWGA 189 Query: 187 IEEGQKTGYFFDQRENRASIAPLIQGWGGRSGITLQEVAAEDGTVQTLPVNKSGKPVTFP 246 Sbjct: 190 R------------------------------------ 213 Query: 247 YWDGATVLECFAHTGSFTLHACKYGAKKVTCLDVSAHAIESAKANVELNGF-SDRVEFVV 305 Sbjct: 214 --- 270 Query: 306 DDAFAFLRNQVKGLEERSERATGKTDAKPGAKPASKTDTAKPMTAGGGRTWDVVILDPPA 365 Sbjct: 271 ---------------------------------- 296 Query: 366 FAKTKSAVAGACRGYKDINLHGMKLVNEGGYLVTASCSYHMQPQLFLDTIAEAAKDAGKV 425 Sbjct: 297 356 Query: 426 LRLIEWRAAGKDHPQILGVDEGHYLKFAIFEV 457 Sbjct: 357 388 >STM1080 [R] COG1092 Predicted SAM-dependent methyltransferases Length = 403 Score = 276 bits (705), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 165/455 (36%), Positives = 237/455 (52%), Gaps = 76/455 (16%) Query: 1 LERSRKKRLEQGHPWVYASEVASVDGDPQPGGLVDVLNHQGRYLGTGYYNPASQIRVRIL 60 L + R+K L + HPWV++ V+ ++G G +D+++HQG++L G ++PASQIR R+ Sbjct: 14 LSKGREKSLLRRHPWVFSGAVSRLEGKANLGETIDIVDHQGKWLARGAWSPASQIRARVW 73 Query: 61 ARNPLAAMDTAFFAGRLASCLRHRQRFLP--GADAYRLVYGEADFLPGLIIDRFGEVLVV 118 + ++D AFF RL + R G D+YRL+ GE+D LPG+ IDRFG LV+ Sbjct: 74 TFDKAESIDIAFFTRRLRQAQQWRDWLAKKDGLDSYRLIAGESDGLPGVTIDRFGHFLVL 133 Query: 119 QLLTLGMDKCRDQIVQALVEVVAPRGIYERSDVSVRELEGLEQRTGVLYGECPRHIT-VS 177 QLL+ G + R ++ AL IY+RSDV+VR+ EG+ G + GE P + + Sbjct: 134 QLLSAGAEYQRAALISALQTCYPDCAIYDRSDVAVRKKEGMALTQGPVTGELPPALLPIE 193 Query: 178 ENGLKVIVDIEEGQKTGYFFDQRENRASIAPLIQGWGGRSGITLQEVAAEDGTVQTLPVN 237 E+G+K++VDI+ G KTGY+ DQR++R + Sbjct: 194 EHGMKLLVDIQGGHKTGYYLDQRDSRLA-------------------------------- 221 Query: 238 KSGKPVTFPYWDGATVLECFAHTGSFTLHACKYGAKKVTCLDVSAHAIESAKANVELNGF 297 T Y + VL CF++TG F + A G ++V +D S A++ A+ NVELN Sbjct: 222 ------TRRYVENQRVLNCFSYTGGFAVSALMGGCRQVVSVDTSQDALDIARQNVELNQL 275 Query: 298 S-DRVEFVVDDAFAFLRNQVKGLEERSERATGKTDAKPGAKPASKTDTAKPMTAGGGRTW 356 + EFV DD F LR E E+ + Sbjct: 276 DLSKAEFVRDDVFKLLR----AYREHGEK------------------------------F 301 Query: 357 DVVILDPPAFAKTKSAVAGACRGYKDINLHGMKLVNEGGYLVTASCSYHMQPQLFLDTIA 416 DV+I+DPP F + KS + GACRGYKDIN+ ++L+N GG L+T SCS M LF IA Sbjct: 302 DVIIMDPPKFVENKSQLMGACRGYKDINMLAIQLLNPGGILLTFSCSGLMTSDLFQKIIA 361 Query: 417 EAAKDAGKVLRLIEWRAAGKDHPQILGVDEGHYLK 451 +AA DAG+ ++ IE DHP I EG YLK Sbjct: 362 DAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK 396 >VC1354 [R] COG1092 Predicted SAM-dependent methyltransferases Length = 418 Score = 270 bits (690), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 168/459 (36%), Positives = 239/459 (52%), Gaps = 83/459 (18%) Query: 1 LERSRKKRLEQGHPWVYASEVASVDGDPQPGGLVDVLNHQGRYLGTGYYNPASQIRVRI- 59 L + R K L + HPWV++ ++ V+G P+ G VDV + +G++L Y+P SQI R+ Sbjct: 28 LVKGRDKSLRRKHPWVFSRGISKVEGTPKLGETVDVYSFEGKWLAKAAYSPHSQITARVW 87 Query: 60 -LARNPLAAMDTAFFAGRL--ASCLRHRQRFLPGADAYRLVYGEADFLPGLIIDRFGEVL 116 + P+ D FF R+ A LR+ G YRL+ E+D LPG+ ID++ + L Sbjct: 88 SFEQEPI---DRDFFIKRIEQAQLLRNDIIERDGLTGYRLIAAESDGLPGITIDKYQDYL 144 Query: 117 VVQLLTLGMDKCRDQIVQALVEVVAPRGIYERSDVSVRELEGLEQRTGVLYGECP-RHIT 175 V QLL+ G + + +V+AL+ IYERSDV+VR+ EGL++R GVL+GE P + + Sbjct: 145 VCQLLSAGAEYQKQTLVEALLHCFPECHIYERSDVAVRKKEGLDERVGVLHGELPPKSVV 204 Query: 176 VSENGLKVIVDIEEGQKTGYFFDQRENRASIAPLIQGWGGRSGITLQEVAAEDGTVQTLP 235 + ENG+K+ VDI G KTG++ DQR++R Sbjct: 205 IEENGVKISVDIVGGHKTGFYLDQRDSRFQ------------------------------ 234 Query: 236 VNKSGKPVTFPYWDGATVLECFAHTGSFTLHACKYGAKKVTCLDVSAHAIESAKANVELN 295 + Y VL CF++TG F L+A K GAK+V DVS A+++AK N ELN Sbjct: 235 --------SMKYVKEKEVLNCFSYTGGFGLYALKGGAKRVINADVSQPALDTAKFNAELN 286 Query: 296 GF---SDRVEFVVDDAFAFLRNQVKGLEERSERATGKTDAKPGAKPASKTDTAKPMTAGG 352 GF R F+ D F LR E R + Sbjct: 287 GFDISKKRAVFLNADVFKLLR------EYRDQ---------------------------- 312 Query: 353 GRTWDVVILDPPAFAKTKSAVAGACRGYKDINLHGMKLVNEGGYLVTASCSYHMQPQLFL 412 G +DVV++DPP FA++K+ + GACRGYKDIN+ M+++N GG L+T SCS M LF Sbjct: 313 GTRFDVVVMDPPKFAESKAQLDGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDQVLFQ 372 Query: 413 DTIAEAAKDAGKVLRLIEWRAAGKDHPQILGVDEGHYLK 451 IA+AA DAG+ ++ +E DHP EG YLK Sbjct: 373 KIIADAALDAGRDVKFVERFEQAADHPTDTAYPEGFYLK 411 >Z1319 [R] COG1092 Predicted SAM-dependent methyltransferases Length = 367 Score = 270 bits (690), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 159/432 (36%), Positives = 228/432 (52%), Gaps = 76/432 (17%) Query: 24 VDGDPQPGGLVDVLNHQGRYLGTGYYNPASQIRVRILARNPLAAMDTAFFAGRLASCLRH 83 ++G G +D+++HQG++L G Y+PASQIR R+ +P ++D AFF+ RL + Sbjct: 1 MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKW 60 Query: 84 RQRFLP--GADAYRLVYGEADFLPGLIIDRFGEVLVVQLLTLGMDKCRDQIVQALVEVVA 141 R G D+YRL+ GE+D LPG+ IDRFG LV+QLL+ G + R ++ AL + Sbjct: 61 RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYP 120 Query: 142 PRGIYERSDVSVRELEGLEQRTGVLYGECPRHIT-VSENGLKVIVDIEEGQKTGYFFDQR 200 IY+RSDV+VR+ EG+E G++ GE P + + E+G+K++VDI+ G KTGY+ DQR Sbjct: 121 ECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR 180 Query: 201 ENRASIAPLIQGWGGRSGITLQEVAAEDGTVQTLPVNKSGKPVTFPYWDGATVLECFAHT 260 ++R + T Y + VL CF++T Sbjct: 181 DSRLA--------------------------------------TRRYVENKRVLNCFSYT 202 Query: 261 GSFTLHACKYGAKKVTCLDVSAHAIESAKANVELNGFS-DRVEFVVDDAFAFLRNQVKGL 319 G F + A G +V +D S A++ A+ NVELN + EFV DD F LR Sbjct: 203 GGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRT----Y 258 Query: 320 EERSERATGKTDAKPGAKPASKTDTAKPMTAGGGRTWDVVILDPPAFAKTKSAVAGACRG 379 +R E+ +DV+++DPP F + KS + GACRG Sbjct: 259 RDRGEK------------------------------FDVIVMDPPKFVENKSQLMGACRG 288 Query: 380 YKDINLHGMKLVNEGGYLVTASCSYHMQPQLFLDTIAEAAKDAGKVLRLIEWRAAGKDHP 439 YKDIN+ ++L+NEGG L+T SCS M LF IA+AA DAG+ ++ IE DHP Sbjct: 289 YKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHP 348 Query: 440 QILGVDEGHYLK 451 I EG YLK Sbjct: 349 VIATYPEGLYLK 360 >ECs1051 [R] COG1092 Predicted SAM-dependent methyltransferases Length = 367 Score = 270 bits (690), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 159/432 (36%), Positives = 228/432 (52%), Gaps = 76/432 (17%) Query: 24 VDGDPQPGGLVDVLNHQGRYLGTGYYNPASQIRVRILARNPLAAMDTAFFAGRLASCLRH 83 ++G G +D+++HQG++L G Y+PASQIR R+ +P ++D AFF+ RL + Sbjct: 1 MEGKASLGETIDIVDHQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKW 60 Query: 84 RQRFLP--GADAYRLVYGEADFLPGLIIDRFGEVLVVQLLTLGMDKCRDQIVQALVEVVA 141 R G D+YRL+ GE+D LPG+ IDRFG LV+QLL+ G + R ++ AL + Sbjct: 61 RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYP 120 Query: 142 PRGIYERSDVSVRELEGLEQRTGVLYGECPRHIT-VSENGLKVIVDIEEGQKTGYFFDQR 200 IY+RSDV+VR+ EG+E G++ GE P + + E+G+K++VDI+ G KTGY+ DQR Sbjct: 121 ECAIYDRSDVAVRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR 180 Query: 201 ENRASIAPLIQGWGGRSGITLQEVAAEDGTVQTLPVNKSGKPVTFPYWDGATVLECFAHT 260 ++R + T Y + VL CF++T Sbjct: 181 DSRLA--------------------------------------TRRYVENKRVLNCFSYT 202 Query: 261 GSFTLHACKYGAKKVTCLDVSAHAIESAKANVELNGFS-DRVEFVVDDAFAFLRNQVKGL 319 G F + A G +V +D S A++ A+ NVELN + EFV DD F LR Sbjct: 203 GGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFVRDDVFKLLRT----Y 258 Query: 320 EERSERATGKTDAKPGAKPASKTDTAKPMTAGGGRTWDVVILDPPAFAKTKSAVAGACRG 379 +R E+ +DV+++DPP F + KS + GACRG Sbjct: 259 RDRGEK------------------------------FDVIVMDPPKFVENKSQLMGACRG 288 Query: 380 YKDINLHGMKLVNEGGYLVTASCSYHMQPQLFLDTIAEAAKDAGKVLRLIEWRAAGKDHP 439 YKDIN+ ++L+NEGG L+T SCS M LF IA+AA DAG+ ++ IE DHP Sbjct: 289 YKDINMLAIQLLNEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHP 348 Query: 440 QILGVDEGHYLK 451 I EG YLK Sbjct: 349 VIATYPEGLYLK 360 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,561,245 Number of extensions: 1522464 Number of successful extensions: 5045 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 5019 Number of HSP's successfully gapped: 37 Length of query: 460 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 350 Effective length of database: 25,691,691 Effective search space: 8992091850 Effective search space used: 8992091850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)