BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0199.0014.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1398 [P] COG0581 ABC-type phosphate transport system, permease... 181 1e-45 VCA0072 [P] COG0581 ABC-type phosphate transport system, permeas... 157 2e-38 MA0889 [P] COG0581 ABC-type phosphate transport system, permease... 130 4e-30 MA3095 [P] COG0581 ABC-type phosphate transport system, permease... 127 2e-29 sll0682 [P] COG0581 ABC-type phosphate transport system, permeas... 115 1e-25 >SP1398 [P] COG0581 ABC-type phosphate transport system, permease component Length = 294 Score = 181 bits (459), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 102/280 (36%), Positives = 152/280 (54%), Gaps = 10/280 (3%) Query: 11 RSQRNNKIANIAFYTLGALVMLLIFWLLFTILSKGLPSLRPDFLIKQPEEIDAGGGIGPV 70 R+++ +K+A YT+ ++++ ++ L+ IL +GLP + FL + AGGGIG Sbjct: 2 RAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSFLTGRSSAFQAGGGIGIQ 61 Query: 71 LXXXXXXXXXXXXXXXXXXXXXXXYMAEYAPDNRFTGALRICVESLSSVPSIVFGLLGLA 130 L Y+AEYA T +R C+E LSS+PS+V GL G Sbjct: 62 LYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRTCIEILSSLPSVVVGLFGYL 121 Query: 131 IFAEYFNVGLTILGGGVSLALLNLPMLARVTEEAVRAVPGEIREAGYALGMTKFHVIRKV 190 IF F G +I+ G ++L + NLP + R E++++ V REAG ALG++++ + V Sbjct: 122 IFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVHHTQREAGLALGISRWETVVHV 181 Query: 191 VLPVAMPAIVTGVCLVAGRAFGESAVIILTAGLSTSGEMWD----------FNLFSPGET 240 V+P A+P IVTGV L +GR FGE+A +I TAG S W ++F ET Sbjct: 182 VIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPALDWSNWNILSVTSPISIFRQAET 241 Query: 241 LAVHLWYVQSEAIVEDARQIADKSAAVLVFVVLLINFMFR 280 LAVH+W V SE + D ++ SAAVL+ +L+ NF R Sbjct: 242 LAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIFNFGAR 281 >VCA0072 [P] COG0581 ABC-type phosphate transport system, permease component Length = 289 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 98/283 (34%), Positives = 146/283 (51%), Gaps = 6/283 (2%) Query: 1 MNFTRTR-HTARSQRNNKIANIAFYTLGALVMLLIFWLLFTILSKGLPSLRPDFLIKQPE 59 M R + AR ++N I + AL + +FW+++ ILS GL + F+ Sbjct: 1 MTMDRVKLKQARVLKDN-ILRTFIWISAALTVGFLFWIIWYILSNGLQHVNWKFITDNYT 59 Query: 60 EIDAGGGIGPVLXXXXXXXXXXXXXXXXXXXXXXXYMAEYAP-DNRFTGALRICVESLSS 118 GI P++ Y+ EYA +R +R C ESL+ Sbjct: 60 HTGDEHGIFPMIVSTVYMVVASIAVAAPIGIMTAIYLTEYAKVGSRLVKIIRFCTESLAG 119 Query: 119 VPSIVFGLLGLAIFAEYFNVGLTILGGGVSLALLNLPMLARVTEEAVRAVPGEIREAGYA 178 +PSI+FGL G+ F +G +IL G ++L++L LP++ R TEEA+ AVP RE Y Sbjct: 120 IPSIIFGLFGMTFFVAILGLGFSILSGALTLSILILPVIIRTTEEALMAVPQTYREGSYG 179 Query: 179 LGMTKFHVIRKVVLPVAMPAIVTGVCLVAGRAFGESAVIILTAGLSTSGEMWDFNLFSPG 238 +G +K + IR+++LP AMP I+T V L GR GESA + LTAG+ + D +L G Sbjct: 180 VGASKIYTIRRLILPSAMPGILTSVILSIGRVIGESAPVFLTAGM--VARIPD-SLLDSG 236 Query: 239 ETLAVHLWYVQSEAIVEDARQIADKSAAVLVFVVLLINFMFRF 281 TL VHL+ + +E + A +A VL+ VVLLIN + + Sbjct: 237 RTLTVHLYKLTTELFTIEEWNQAYGTATVLIVVVLLINMITKL 279 >MA0889 [P] COG0581 ABC-type phosphate transport system, permease component Length = 307 Score = 130 bits (326), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 88/260 (33%), Positives = 137/260 (52%), Gaps = 5/260 (1%) Query: 21 IAFYTLGALVMLL---IFWLLFTILSKGLPSLRPDFLIKQPEEIDAGGGIGPVLXXXXXX 77 IAF LG ML+ + +L I+ G ++ +FL + P GGI P + Sbjct: 33 IAFVLLGLSAMLVAGFVVIILAYIVYNGYSAINVEFLTEIPRMRMTQGGIYPAIVGTVLL 92 Query: 78 XXXXXXXXXXXXXXXXXYMAEYAPDNRFTGALRICVESLSSVPSIVFGLLGLAIFAEYFN 137 Y+ EYA + R T + + + +L+ PS+VFGL GLA+F +YF Sbjct: 93 IIGSMAVALPLGILAAVYLNEYAGETRTTWLIEMAINNLAGTPSVVFGLFGLALFVKYFG 152 Query: 138 VGLTILGGGVSLALLNLPMLARVTEEAVRAVPGEIREAGYALGMTKFHVIRKVVLPVAMP 197 G +IL ++L+ L +P++ R ++EA+ AVP E RE+ ALG++K+ IR VVLP A+P Sbjct: 153 FGSSILSASLTLSFLIIPVIIRSSQEALIAVPKEYRESSLALGISKWQTIRHVVLPAAIP 212 Query: 198 AIVTGVCLVAGRAFGESAVIILTAGLSTSGEMWDFNLFSPGETLAVHLWYVQSEAI-VED 256 IVTG L GR GE+A I+LT + D +++S L HL+ + + + Sbjct: 213 GIVTGSILSIGRVAGETAPILLTGAAYFLPRLPD-SIYSQFMALPYHLFVLATSGTSIVK 271 Query: 257 ARQIADKSAAVLVFVVLLIN 276 R + +A VL+ +VL +N Sbjct: 272 TRPLQYGTALVLLMIVLGMN 291 >MA3095 [P] COG0581 ABC-type phosphate transport system, permease component Length = 300 Score = 127 bits (319), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 99/296 (33%), Positives = 151/296 (51%), Gaps = 21/296 (7%) Query: 11 RSQRNNKIANIAFYTLGALVMLLIFWLLFTILSKGLPSLRPDFLIKQPEEIDA-GGGIGP 69 R + N I++++ +V++L+F + I KG+PSL P+F+ ++ GG IG Sbjct: 7 RILKGNIFMGISYFSAATVVLVLLFIIG-IIAIKGIPSLTPEFVFSSESQVFGFGGAIGN 65 Query: 70 VLXXXXXXXXXXXXXXXXXXXXXXXYMAEYAPDNRFTGALRICVESLSSVPSIVFGLLG- 128 + Y+ YA + FT +L +E LS PSIV GL G Sbjct: 66 AIVGTILLSTLSPLLAIPFAIGTAIYLKRYAKEGFFTSSLSFLLEVLSGTPSIVLGLFGF 125 Query: 129 --LAIFAEYFNVGLTILGGGVSLALLNLPMLARVTEEAVRAVPGEIREAGYALGMTKFHV 186 LAI +YF G +++ G ++LA+L LP++ + TE A+ +VP E+ +A YALG TK+ Sbjct: 126 LFLAIQMKYFTGGFSLISGTIALAILVLPVMEKATENALDSVPLELEDASYALGATKWET 185 Query: 187 IRKVVLPVAMPAIVTGVCLVAGRAFGESAVIILTAGLSTSGEMW-----DFNLFS----P 237 I K+ LP A+ I+TG+ L GRA ESAV++LTAG S W D +F P Sbjct: 186 ISKISLPYAILGIITGILLSIGRAAEESAVVVLTAGYSQFFPEWKVVPNDKFIFGVKIYP 245 Query: 238 GETLAVHLWYVQSEAIVEDARQIADK----SAAVLVFVVLLINFMFRFPLWLGRRR 289 + L L E ++ +A VL+ +V+LINF R ++L RR+ Sbjct: 246 FQDLVAALPITVYHGF-EFPNMVSPSESFGAAFVLILIVMLINFTTR--MFLRRRK 298 >sll0682 [P] COG0581 ABC-type phosphate transport system, permease component Length = 287 Score = 115 bits (287), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 87/282 (30%), Positives = 138/282 (48%), Gaps = 6/282 (2%) Query: 1 MNFTRTRHTARSQRNNKIANIAFYTLGALVMLLIFWLLFTILSKGLPSLRPDFLIKQPEE 60 +N + + R+ + ++ L A V+ L L+F + +G SL + +K P Sbjct: 4 VNLQKKKSDLRAIFGYSMTAVSAACLLATVIPLFAVLIFVAI-QGFRSLNLNLFLKLPPA 62 Query: 61 ID-AGGGIGPVLXXXXXXXXXXXXXXXXXXXXXXXYMAEYAPDNRFTGALRICVESLSSV 119 AGGG+G + Y++E++ DN+ A+R LS + Sbjct: 63 PGLAGGGVGNAIIGTFIVVAIATVIAVPIGVLSAVYLSEFSGDNQVARAVRFATNLLSGI 122 Query: 120 PSIVFGLLGLAIFAEYFNVGLTILGGGVSLALLNLPMLARVTEEAVRAVPGEIREAGYAL 179 PSI+ G+ G + + GGV+LA+L LP + R T+EA++ VP +IR A + Sbjct: 123 PSIIAGVFAYGALVSSGLFGFSAIAGGVALAVLMLPTIIRTTDEALQIVPQDIRWAALGV 182 Query: 180 GMTKFHVIRKVVLPVAMPAIVTGVCLVAGRAFGESAVIILTAGLSTSGEMWDFNLFSPGE 239 G K+ + VVLP A+ +I+TGV L RA GE+A +I TA S W L P Sbjct: 183 GAYKYQTVLFVVLPAALSSIITGVTLAIARAAGETAPLIFTALYS---NFWPRGLKEPIA 239 Query: 240 TLAVHLWYVQSEAIVEDARQIADKSAAVLVFVVLLINFMFRF 281 TLAV L Y + + +++A ++ +LVF+VL+ N RF Sbjct: 240 TLAV-LVYNFASVPYKSQQELAWAASLLLVFLVLITNITARF 280 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.142 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,198,134 Number of extensions: 666663 Number of successful extensions: 2758 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 2749 Number of HSP's successfully gapped: 51 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)