BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0199.0008.p (324 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0925 [V] COG0842 ABC-type multidrug transport system, permeas... 224 2e-58 L51437 [V] COG0842 ABC-type multidrug transport system, permease... 155 6e-38 Rv1686c [V] COG0842 ABC-type multidrug transport system, permeas... 84 3e-16 MT1726 [V] COG0842 ABC-type multidrug transport system, permease... 84 3e-16 RSc0165 [V] COG0842 ABC-type multidrug transport system, permeas... 79 1e-14 >lin0925 [V] COG0842 ABC-type multidrug transport system, permease component Length = 371 Score = 224 bits (571), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 130/351 (37%), Positives = 195/351 (55%), Gaps = 27/351 (7%) Query: 1 MALMFIAPLLVLSLMSLVFNGDAYEPKIGVSGGAVPFTRALEAQNATVIAYESADEGKAA 60 +ALMF+APLL+++L++ +F GD +P +GV+G + + L+ + T+ Y A Sbjct: 21 LALMFLAPLLLITLLTYLFEGDTVKPVVGVNGLSSSMVKELKENDLTIKTYPDNTNATAK 80 Query: 61 LQESELDALITLNGTTPEVMLEGSNPXXXXXXXXXXXXXXXXXQSAGGEGQ--------- 111 +++++LDA +G +V E S P Q + Q Sbjct: 81 IKDADLDAFFQQDGEKIKVTYENSEPSLSKEIGLKLQKALMAEQQVQVKKQAKTTGEALA 140 Query: 112 ---IQPK---------------ISYLYGAEDMKTIDRFGPIMIGXXXXXXXXLIAGVSFL 153 I P Y+YG +D D PI IG LIAG+SFL Sbjct: 141 KAGIDPNSLPALTNSPEKLTISTDYVYGDKDTSFFDTISPIFIGFFVFFFVFLIAGISFL 200 Query: 154 RERTTGTLERLLSTPLKRWEIVLGYVCGFGIFTVFQALLISWFSIQVLGIMMAGSFGYVX 213 RERTTGTLERL++TP+KR E+ LGY+ GFGIF + Q++L++ FSIQVLG+M GS YV Sbjct: 201 RERTTGTLERLMATPIKRIELELGYLIGFGIFALLQSILVAVFSIQVLGMMQNGSLFYVL 260 Query: 214 XXXXXXXXXXXXXXXXXXXYASNELQMIQFIPLVIVPQIFLSGLFPLDTLPLWLQRVGLA 273 +A+NE Q++QFIP+VIVPQ+ G+FPLD + WL + Sbjct: 261 LITLTLGMVSLALGILLSTFANNEFQIVQFIPIVIVPQVLFCGIFPLDGMADWLVWIAHI 320 Query: 274 TPIYYGSEALMNIMIRGKGWSAIALDVYALIGFSLLFMLLNVLALRKHRKM 324 P+YYG+ AL +IM++G+G+++ A D Y L+GF ++F++LN+ AL+K+RK+ Sbjct: 321 MPLYYGANALTSIMVKGEGFASFANDFYILLGFVIIFVVLNIFALKKYRKV 371 >L51437 [V] COG0842 ABC-type multidrug transport system, permease component Length = 380 Score = 155 bits (393), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 108/361 (29%), Positives = 168/361 (46%), Gaps = 40/361 (11%) Query: 1 MALMFIAPLLVLSLMSLVF---NGDAYEPKIGVSGGAVPFTRALE---AQNATVIAYESA 54 +A+MF+AP+ +L+LM +F + +AY ++G+ TRAL+ A + + Sbjct: 21 LAMMFLAPIFILTLMYFLFQVPSNNAY--RVGLKSDNTELTRALKDMPNDKAHLKVISVS 78 Query: 55 DEGKAALQESELDALITLNGTTPEVMLEG-SNPXXXXXXXXXXXXXXXXXQSAGGEGQIQ 113 + ++ + E +L A+++++G +V SN A E Q Sbjct: 79 ENNRSTMNEKDLSAIVSISGKNIDVSYANQSNGTSSAIKLLLTQVIQESQVKATKEETKQ 138 Query: 114 ---------PKIS--------------------YLYG--AEDMKTIDRFGPIMIGXXXXX 142 P+ S YLYG + T D PI++G Sbjct: 139 ALQNLISKLPQTSGQSFSTNFRVDTEQYKVTSHYLYGDPSSTNATFDDLAPILVGAFAFF 198 Query: 143 XXXLIAGVSFLRERTTGTLERLLSTPLKRWEIVLGYVCGFGIFTVFQALLISWFSIQVLG 202 LI+G+S + ERT+G L R+L TP+KR EIV GY +GI + Q L+ F+ +LG Sbjct: 199 LVLLISGISLVNERTSGMLGRMLVTPVKRTEIVTGYTLSYGILAMIQTALMVVFTYWILG 258 Query: 203 IMMAGSFGYVXXXXXXXXXXXXXXXXXXXXYASNELQMIQFIPLVIVPQIFLSGLFPLDT 262 + G+ G+V A E Q +Q IPL IVPQ SGL P+DT Sbjct: 259 VHHNGNIGWVFVINFFIAIIALLFGLLLSTLAKTEFQFVQMIPLAIVPQFLFSGLIPVDT 318 Query: 263 LPLWLQRVGLATPIYYGSEALMNIMIRGKGWSAIALDVYALIGFSLLFMLLNVLALRKHR 322 +P+ LQ + P+YYG A+ ++ RG G+S I D+ ++ F+ L L NVLAL+ R Sbjct: 319 MPVILQAIAHCIPVYYGISAMQEVVKRGVGFSGIWFDLLIMLLFAALLYLANVLALKNVR 378 Query: 323 K 323 + Sbjct: 379 R 379 >Rv1686c [V] COG0842 ABC-type multidrug transport system, permease component Length = 226 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 57/189 (30%), Positives = 90/189 (47%), Gaps = 1/189 (0%) Query: 134 IMIGXXXXXXXXLIAGVSFLRERTTGTLERLLSTPLKRWEIVLGYVCGFGIFTVFQALLI 193 +++G +I ++ RER +GTLER+L+TPL+R +++ GY F I QA L Sbjct: 36 VLLGLFPLFVMFVITAITMQRERASGTLERILTTPLRRLDLLAGYGTAFSIAAAAQATLA 95 Query: 194 SWFSIQVLGIMMAGSFGYVXXXXXXXXXXXXXXXXXXXXYASNELQMIQFIPLVIVPQIF 253 + LG AGS +V +A E Q +QFIPLV+VPQ+ Sbjct: 96 CIVAFWFLGFDTAGSPVWVFAIAIVNAVLGVGLGLLCSAFARTEFQAVQFIPLVMVPQLL 155 Query: 254 LSGLF-PLDTLPLWLQRVGLATPIYYGSEALMNIMIRGKGWSAIALDVYALIGFSLLFML 312 L+G+ P +P WL+ + P Y EAL + + DV ++ F++ + Sbjct: 156 LAGIIVPRALMPTWLEWISNVMPASYALEALQQVGAHPELTGIAVRDVVVVLSFAVASLC 215 Query: 313 LNVLALRKH 321 L + LR+ Sbjct: 216 LAAVTLRRR 224 >MT1726 [V] COG0842 ABC-type multidrug transport system, permease component Length = 226 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 57/189 (30%), Positives = 90/189 (47%), Gaps = 1/189 (0%) Query: 134 IMIGXXXXXXXXLIAGVSFLRERTTGTLERLLSTPLKRWEIVLGYVCGFGIFTVFQALLI 193 +++G +I ++ RER +GTLER+L+TPL+R +++ GY F I QA L Sbjct: 36 VLLGLFPLFVMFVITAITMQRERASGTLERILTTPLRRLDLLAGYGTAFSIAAAAQATLA 95 Query: 194 SWFSIQVLGIMMAGSFGYVXXXXXXXXXXXXXXXXXXXXYASNELQMIQFIPLVIVPQIF 253 + LG AGS +V +A E Q +QFIPLV+VPQ+ Sbjct: 96 CIVAFWFLGFDTAGSPVWVFAIAIVNAVLGVGLGLLCSAFARTEFQAVQFIPLVMVPQLL 155 Query: 254 LSGLF-PLDTLPLWLQRVGLATPIYYGSEALMNIMIRGKGWSAIALDVYALIGFSLLFML 312 L+G+ P +P WL+ + P Y EAL + + DV ++ F++ + Sbjct: 156 LAGIIVPRALMPTWLEWISNVMPASYALEALQQVGAHPELTGIAVRDVVVVLSFAVASLC 215 Query: 313 LNVLALRKH 321 L + LR+ Sbjct: 216 LAAVTLRRR 224 >RSc0165 [V] COG0842 ABC-type multidrug transport system, permease component Length = 381 Score = 78.6 bits (192), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 81/351 (23%), Positives = 141/351 (40%), Gaps = 33/351 (9%) Query: 2 ALMFIAPLLVLSLMSLVFNGDAYEPKIGV-SGGAVPFTRALEA--QNATVIAYESA--DE 56 A+M P++ L + N D GV + FTR+ A +N + DE Sbjct: 32 AMMIGIPIMQLLMFGFAINTDPKHLPTGVIAADRSEFTRSFLASLRNTDYFRLDETLPDE 91 Query: 57 --GKAALQESELDALITL---------NGTTPEVMLEG--SNPXXXXXXXXXXXXXXXXX 103 G+ AL E L ++++ G P +++E ++P Sbjct: 92 AAGRQALAEGRLQFVVSIPPDFTRRLVRGERPALLVEADATDPSATGLALASVTQLAQGV 151 Query: 104 QS----------AGGEGQIQPKISYLYGAEDMKTIDRFGPIMIGXXXXXXXXLIAGVSFL 153 S AGG+ + ++ LY E + + P ++G ++ G++ Sbjct: 152 VSKDMKGVLAPLAGGQPPFEVRVHRLYNPEGITQYNII-PGLMGVILTMTMVMMTGLAMT 210 Query: 154 RERTTGTLERLLSTPLKRWEIVLGYVCGFGIFTVFQALLISWFSIQVLGIMMAGSFGYVX 213 RER GT+E LL+TP++ E++ G + + + Q +I + + + GS V Sbjct: 211 RERERGTMENLLATPVRPLEVMTGKIVPYIFIGLVQVSIILLAARFIFHVPFVGSAWMVY 270 Query: 214 XXXXXXXXXXXXXXXXXXXYASNELQMIQFIPLVIVPQIFLSG-LFPLDTLPLWLQRVGL 272 A N+LQ Q +P I LSG +FP +P W Q +G Sbjct: 271 LAALLFIVASLTVGITLSSLAQNQLQATQLTFFYFLPSILLSGFMFPFAGMPKWAQVIGD 330 Query: 273 ATPIYYGSEALMNIMIRGKGWSAIALDVYALIGFSLLFMLLNVLALRKHRK 323 P+ Y I+++G GW + ++ L+ F+ + M +ALR +RK Sbjct: 331 VLPMTYFHRLTRGILLKGNGWVELWPSIWPLLVFTAVVM---GIALRFYRK 378 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.141 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,045,904 Number of extensions: 716371 Number of successful extensions: 3131 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 3130 Number of HSP's successfully gapped: 32 Length of query: 324 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 217 Effective length of database: 26,037,693 Effective search space: 5650179381 Effective search space used: 5650179381 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 116 (49.3 bits)